{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:54:38Z","timestamp":1788836078062,"version":"build-2803163510"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002842","name":"Chiang Mai University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002842","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022893","name":"Chiang Mai University Faculty of Associated Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100022893","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Global Antimicrobial Resistance"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.jgar.2025.07.024","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:17:45Z","timestamp":1753888665000},"page":"371-378","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Genotypic and phenotypic characterization of vancomycin-resistant enterococcus faecium from clinical and surveillance samples in northern Thailand"],"prefix":"10.1016","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4301-4683","authenticated-orcid":false,"given":"Natthida","family":"Nokkhiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3251-8020","authenticated-orcid":false,"given":"Papatsara","family":"Jitta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasarat","family":"Lonok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phadungkiat","family":"Khamnoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usanee","family":"Wattananandkul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina","family":"Guernier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukanya","family":"Saikaew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathupakorn","family":"Dechsupa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1329-7264","authenticated-orcid":false,"given":"Maytawan","family":"Thanunchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgar.2025.07.024_bib0001","unstructured":"T.E. Said MS, Lesho E. Enterococcus Infections. 2025 [cited 2025 March 5]; Available from: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK567759\/."},{"key":"10.1016\/j.jgar.2025.07.024_bib0002","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.2147\/IDR.S206084","article-title":"Global prevalence of antibiotic resistance in blood-isolated Enterococcus faecalis and Enterococcus faecium: a systematic review and meta-analysis Infect","volume":"12","author":"Jabbari Shiadeh","year":"2019","journal-title":"Drug Resist"},{"issue":"5","key":"10.1016\/j.jgar.2025.07.024_bib0003","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1089\/mdr.2017.0147","article-title":"Vancomycin-resistant enterococci: a review of antimicrobial resistance mechanisms and perspectives of Human and animal health","volume":"24","author":"Ahmed","year":"2018","journal-title":"Microb Drug Resist"},{"issue":"10","key":"10.1016\/j.jgar.2025.07.024_bib0004","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1586\/14787210.2014.956092","article-title":"Mechanisms of antibiotic resistance in enterococci","volume":"12","author":"Miller","year":"2014","journal-title":"Expert Rev Anti Infect Ther"},{"key":"10.1016\/j.jgar.2025.07.024_bib0005","unstructured":"World Health Organization. WHO bacterial priority pathogens list, 2024. [cited 2025 March 5]; Available from: https:\/\/www.who.int\/publications\/i\/item\/9789240093461."},{"key":"10.1016\/j.jgar.2025.07.024_bib0006","unstructured":"National Antimicrobial Resistant Surveillance Center. Thailand. Antimicrobial Resistance. [cited 2025 March 5]; Available from: http:\/\/narst.dmsc.moph.go.th\/."},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0007","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ijantimicag.2007.08.026","article-title":"Vancomycin-resistant enterococci (VRE): transmission and control","volume":"31","author":"Tacconelli","year":"2008","journal-title":"Int J Antimicrob Agents"},{"key":"10.1016\/j.jgar.2025.07.024_bib0008","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jgar.2020.02.012","article-title":"Prospective infection surveillance and systematic screening for vancomycin-resistant enterococci in hematologic and oncologic patients - findings of a German tertiary care center","volume":"22","author":"Chhatwal","year":"2020","journal-title":"J Glob Antimicrob Resist"},{"issue":"7","key":"10.1016\/j.jgar.2025.07.024_bib0009","article-title":"Enterococcus virulence and resistant traits associated with its permanence in the hospital environment","volume":"11","author":"Geraldes","year":"2022","journal-title":"Antibiotics (Basel)"},{"issue":"22","key":"10.1016\/j.jgar.2025.07.024_bib0010","doi-asserted-by":"crossref","first-page":"8233","DOI":"10.1128\/JB.01205-07","article-title":"Enterococcal surface protein esp is important for biofilm formation of Enterococcus faecium E1162","volume":"189","author":"Heikens","year":"2007","journal-title":"J Bacteriol"},{"issue":"9","key":"10.1016\/j.jgar.2025.07.024_bib0011","doi-asserted-by":"crossref","first-page":"4110","DOI":"10.1128\/IAI.00375-08","article-title":"A functional collagen adhesin gene, acm, in clinical isolates of Enterococcus faecium correlates with the recent success of this emerging nosocomial pathogen","volume":"76","author":"Nallapareddy","year":"2008","journal-title":"Infect Immun"},{"issue":"9","key":"10.1016\/j.jgar.2025.07.024_bib0012","doi-asserted-by":"crossref","first-page":"5033","DOI":"10.1128\/IAI.71.9.5033-5041.2003","article-title":"An Enterococcus faecium secreted antigen, SagA, exhibits broad-spectrum binding to extracellular matrix proteins and appears essential for E. faecium growth","volume":"71","author":"Teng","year":"2003","journal-title":"Infect Immun"},{"issue":"3","key":"10.1016\/j.jgar.2025.07.024_bib0013","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1086\/367711","article-title":"A potential virulence gene, hylEfm, predominates in Enterococcus faecium of clinical origin","volume":"187","author":"Rice","year":"2003","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jgar.2025.07.024_bib0014","unstructured":"Clinical Lab Standards Institute (CLSI), M100 performance standards for antimicrobial susceptibility testing. 2023: Clinical and Laboratory Standards Institute, USA."},{"issue":"8","key":"10.1016\/j.jgar.2025.07.024_bib0015","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens10080996","article-title":"Direct detection of Streptococcus suis from cerebrospinal fluid, positive hemoculture, and simultaneous differentiation of serotypes 1, 1\/2, 2, and 14 within single reaction","volume":"10","author":"Thu","year":"2021","journal-title":"Pathogens"},{"issue":"8","key":"10.1016\/j.jgar.2025.07.024_bib0016","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","article-title":"Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci","volume":"115","author":"Stepanovic","year":"2007","journal-title":"APMIS"},{"key":"10.1016\/j.jgar.2025.07.024_bib0017","author":"Andrews","year":"2010","journal-title":"FastQC: a quality control tool for high throughput sequence data"},{"issue":"6","key":"10.1016\/j.jgar.2025.07.024_bib0018","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1005595","article-title":"Unicycler: resolving bacterial genome assemblies from short and long sequencing reads","volume":"13","author":"Wick","year":"2017","journal-title":"PLoS Comput Biol"},{"key":"10.1016\/j.jgar.2025.07.024_bib0019","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/978-1-4939-9877-7_20","article-title":"PlasmidFinder and In Silico pMLST: identification and typing of plasmid replicons in whole-genome sequencing (WGS)","volume":"2075","author":"Carattoli","year":"2020","journal-title":"Methods Mol Biol"},{"issue":"11","key":"10.1016\/j.jgar.2025.07.024_bib0020","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J Antimicrob Chemother"},{"issue":"D1","key":"10.1016\/j.jgar.2025.07.024_bib0021","doi-asserted-by":"crossref","first-page":"D566","DOI":"10.1093\/nar\/gkw1004","article-title":"CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database","volume":"45","author":"Jia","year":"2017","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"10.1016\/j.jgar.2025.07.024_bib0022","doi-asserted-by":"crossref","first-page":"D687","DOI":"10.1093\/nar\/gky1080","article-title":"VFDB 2019: A comparative pathogenomic platform with an interactive web interface","volume":"47","author":"Liu","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"10.1016\/j.jgar.2025.07.024_bib0023","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J Clin Microbiol"},{"issue":"6","key":"10.1016\/j.jgar.2025.07.024_bib0024","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1128\/JCM.40.6.1963-1971.2002","article-title":"Multilocus sequence typing scheme for Enterococcus faecium","volume":"40","author":"Homan","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"02","key":"10.1016\/j.jgar.2025.07.024_bib0025","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3855\/jidc.4126","article-title":"Emergence of vanA vancomycin-resistant Enterococcus faecium in a hospital in Porto Alegre, South Brazil","volume":"8","author":"Resende","year":"2014","journal-title":"J Infect Develop Countries"},{"key":"10.1016\/j.jgar.2025.07.024_bib0026","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.11478","article-title":"Molecular epidemiology of vancomycin-resistant Enterococcus faecium clinical isolates in a tertiary care hospital in southern Thailand: a retrospective study","volume":"9","author":"Saengsuwan","year":"2021","journal-title":"PeerJ"},{"issue":"4","key":"10.1016\/j.jgar.2025.07.024_bib0027","article-title":"Enterococcal urinary tract infections: a review of the pathogenicity, epidemiology, and treatment","volume":"12","author":"Codelia-Anjum","year":"2023","journal-title":"Antibiotics (Basel)"},{"issue":"1","key":"10.1016\/j.jgar.2025.07.024_bib0028","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1186\/s12877-019-1398-2","article-title":"Risk factors for vancomycin-resistant enterococcus acquisition during a large outbreak in patients aged 65 years and older","volume":"19","author":"Mathis","year":"2019","journal-title":"BMC Geriatr"},{"key":"10.1016\/j.jgar.2025.07.024_bib0029","doi-asserted-by":"crossref","DOI":"10.3389\/fitd.2024.1307379","article-title":"Antimicrobial resistance profile of Enterococcus species and molecular characterization of Vancomycin resistant Enterococcus faecium from the fecal samples of newly diagnosed adult HIV patients in Dar es Salaam, Tanzania","volume":"5","author":"Kibwana","year":"2024","journal-title":"Frontiers in Tropical Diseases"},{"issue":"3","key":"10.1016\/j.jgar.2025.07.024_bib0030","first-page":"207","article-title":"Virulence factors in high-level gentamicin resistant Enterococcus faecalis isolates from hospitalized patients Srinagarind","volume":"26","author":"Seubwai","year":"2013","journal-title":"Med J"},{"key":"10.1016\/j.jgar.2025.07.024_bib0031","first-page":"14","article-title":"et al.,Genomic characterization of vancomycin-resistant Enterococcus faecium clonal complex 17 isolated from urine in tertiary hospitals in Northeastern Thailand","author":"Chopjitt","year":"2024","journal-title":"Front Microbiol"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0032","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1093\/jac\/dkx387","article-title":"Distribution of putative virulence markers in Enterococcus faecium: towards a safety profile review","volume":"73","author":"Freitas","year":"2018","journal-title":"J Antimicrob Chemother"},{"issue":"6","key":"10.1016\/j.jgar.2025.07.024_bib0033","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1089\/mdr.2018.0320","article-title":"Incidence of virulence determinants among enterococcal clinical isolates in Egypt and its association with biofilm formation","volume":"25","author":"Aladarose","year":"2019","journal-title":"Microbial Drug Resistance"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0034","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00154-13","article-title":"Enterococcus faecium biofilm formation: identification of major autolysin AtlAEfm, associated Acm surface localization, and AtlAEfm-independent extracellular DNA Release","volume":"4","author":"Paganelli","year":"2013","journal-title":"mBio"},{"issue":"19","key":"10.1016\/j.jgar.2025.07.024_bib0035","doi-asserted-by":"crossref","first-page":"6873","DOI":"10.1128\/AEM.01716-15","article-title":"Distinct SagA from hospital-associated clade A1 Enterococcus faecium strains contributes to biofilm formation","volume":"81","author":"Paganelli","year":"2015","journal-title":"Appl Environ Microbiol"},{"issue":"10","key":"10.1016\/j.jgar.2025.07.024_bib0036","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0185920","article-title":"Genetic characteristics and molecular epidemiology of vancomycin-resistant enterococci isolates from Caribbean countries","volume":"12","author":"Akpaka","year":"2017","journal-title":"PLoS ONE"},{"issue":"6","key":"10.1016\/j.jgar.2025.07.024_bib0037","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0235160","article-title":"Comprehensive integrated NGS-based surveillance and contact-network modeling unravels transmission dynamics of vancomycin-resistant enterococci in a high-risk population within a tertiary care hospital","volume":"15","author":"Neumann","year":"2020","journal-title":"PLoS ONE"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0228781","article-title":"A three-year whole genome sequencing perspective of Enterococcus faecium sepsis in Australia","volume":"15","author":"Lee","year":"2020","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jgar.2025.07.024_bib0039","doi-asserted-by":"crossref","first-page":"1278835","DOI":"10.3389\/fmicb.2023.1278835","article-title":"Genomic characterization of vancomycin-resistant Enterococcus faecium clonal complex 17 isolated from urine in tertiary hospitals in Northeastern Thailand","volume":"14","author":"Chopjitt","year":"2023","journal-title":"Front Microbiol"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0040","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ijantimicag.2009.10.006","article-title":"Spread of vancomycin-resistant Enterococcus faecium in two haematological centres in Russia","volume":"35","author":"Brilliantova","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"issue":"3","key":"10.1016\/j.jgar.2025.07.024_bib0041","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1007\/s10123-023-00441-2","article-title":"Molecular characterization and biofilm formation ability of Enterococcus faecium and Enterococcus faecalis bloodstream isolates from a Chinese tertiary hospital in Beijing","volume":"27","author":"Yang","year":"2024","journal-title":"Int Microbiol"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0042","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1086\/321815","article-title":"Vancomycin-resistant enterococci: mechanisms and clinical observations","volume":"33","author":"Gold","year":"2001","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jgar.2025.07.024_bib0043","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jgar.2017.09.009","article-title":"Resistance mechanisms and clinical characteristics of linezolid-resistant Enterococcus faecium isolates: a single-centre study in South Korea","volume":"12","author":"Cho","year":"2018","journal-title":"J Glob Antimicrob Resist"},{"issue":"4","key":"10.1016\/j.jgar.2025.07.024_bib0044","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.idc.2020.08.004","article-title":"Resistance in vancomycin-resistant enterococci","volume":"34","author":"Miller","year":"2020","journal-title":"Infect. Dis. Clin. North Am."},{"key":"10.1016\/j.jgar.2025.07.024_bib0045","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1471-2334-11-80","article-title":"Witte,IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium","volume":"11","author":"Werner","year":"2011","journal-title":"BMC Infect Dis"},{"issue":"2","key":"10.1016\/j.jgar.2025.07.024_bib0046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0030319","article-title":"Hospital and community ampicillin-resistant Enterococcus faecium are evolutionarily closely linked but have diversified through niche adaptation","volume":"7","author":"de Regt","year":"2012","journal-title":"PLoS ONE"},{"issue":"3","key":"10.1016\/j.jgar.2025.07.024_bib0047","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1089\/mdr.2014.0077","article-title":"Different VanA elements in E. faecalis and in E. faecium suggest at least two origins of Tn1546 among VRE in a Brazilian hospital","volume":"21","author":"Merlo","year":"2015","journal-title":"Microb Drug Resist"},{"key":"10.1016\/j.jgar.2025.07.024_bib0048","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.micpath.2017.02.041","article-title":"Molecular assessment of virulence determinants, hospital associated marker (IS16gene) and prevalence of antibiotic resistance in soil borne Enterococcus species","volume":"105","author":"Ali","year":"2017","journal-title":"Microb Pathog"},{"key":"10.1016\/j.jgar.2025.07.024_bib0049","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2024.1466990","article-title":"Genomic analysis of Enterococcus faecium from non-clinical settings: antimicrobial resistance, virulence, and clonal population in livestock and the urban environment","volume":"15","author":"Lopes","year":"2024","journal-title":"Front Microbiol"}],"container-title":["Journal of Global Antimicrobial Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S221371652500181X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S221371652500181X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:15:22Z","timestamp":1777101322000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S221371652500181X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":49,"alternative-id":["S221371652500181X"],"URL":"https:\/\/doi.org\/10.1016\/j.jgar.2025.07.024","relation":{},"ISSN":["2213-7165"],"issn-type":[{"value":"2213-7165","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genotypic and phenotypic characterization of vancomycin-resistant enterococcus faecium from clinical and surveillance samples in northern Thailand","name":"articletitle","label":"Article Title"},{"value":"Journal of Global Antimicrobial Resistance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgar.2025.07.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.","name":"copyright","label":"Copyright"}]}}