{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:10:43Z","timestamp":1785417043764,"version":"3.56.0"},"reference-count":72,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007787","name":"National Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003009","name":"STDF","doi-asserted-by":"publisher","award":["43285"],"award-info":[{"award-number":["43285"]}],"id":[{"id":"10.13039\/501100003009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Genetic Engineering and Biotechnology"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jgeb.2025.100583","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T07:51:17Z","timestamp":1759996277000},"page":"100583","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessment of peripheral gene expression signatures as predictive biomarkers for hepatocellular carcinoma following DAA treatment"],"prefix":"10.1016","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8277-8304","authenticated-orcid":false,"given":"Rehab I.","family":"Moustafa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3537-2408","authenticated-orcid":false,"given":"Sally","family":"Farouk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha G.","family":"Bader El Din","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0928-4738","authenticated-orcid":false,"given":"Hend I.","family":"Shousha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Khairy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9863-5551","authenticated-orcid":false,"given":"Yasser K.","family":"Elesnawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba","family":"Shawky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Gabr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf O.","family":"Abdelaziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr","family":"Abdelaal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8842-6975","authenticated-orcid":false,"given":"Hassan","family":"Elsayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgeb.2025.100583_b0005","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"10.1016\/j.jgeb.2025.100583_b0010","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.jhep.2023.03.017","article-title":"Global burden of liver disease: 2023 update","volume":"79","author":"Devarbhavi","year":"2023","journal-title":"J Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0015","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/s41575-019-0186-y","article-title":"A global view of hepatocellular carcinoma: trends, risk, prevention and management","volume":"16","author":"Yang","year":"2019","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0020","unstructured":"WHO, 2024. Global hepatitis report 2024: action for access in low- and middle-income countries [WWW Document]. URL https:\/\/www.who.int\/publications\/i\/item\/9789240091672 (accessed 11.1.24)."},{"key":"10.1016\/j.jgeb.2025.100583_b0025","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1053\/j.gastro.2018.04.008","article-title":"Incidence of hepatocellular carcinoma in patients with HCV-associated cirrhosis treated with direct-acting antiviral agents","volume":"155","author":"Calvaruso","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jgeb.2025.100583_b0030","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/jvh.13295","article-title":"The impact of SVR from direct-acting antiviral- and interferon-based treatments for HCV on hepatocellular carcinoma risk","volume":"27","author":"Janjua","year":"2020","journal-title":"J Viral Hepat"},{"key":"10.1016\/j.jgeb.2025.100583_b0035","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1053\/j.gastro.2019.04.033","article-title":"Indelibly stamped by hepatitis C virus infection: persistent epigenetic signatures increasing liver cancer risk","volume":"156","author":"Lohmann","year":"2019","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jgeb.2025.100583_b0040","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3390\/livers1040024","article-title":"Predictive factors for hepatocellular carcinoma development after direct-acting antiviral treatment of HCV","volume":"1","author":"Macek Jilkova","year":"2021","journal-title":"Livers"},{"key":"10.1016\/j.jgeb.2025.100583_b0045","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1053\/j.gastro.2018.07.015","article-title":"Incidence of hepatocellular carcinoma after direct antiviral therapy for HCV in patients with cirrhosis included in surveillance programs","volume":"155","author":"Nahon","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jgeb.2025.100583_b0050","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.jhep.2018.03.009","article-title":"Newly diagnosed hepatocellular carcinoma in patients with advanced hepatitis C treated with DAAs: a prospective population study","volume":"69","author":"Romano","year":"2018","journal-title":"J Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0055","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1111\/jvh.13276","article-title":"Incidence of HCC in chronic hepatitis C patients with advanced hepatic fibrosis who achieved SVR following DAAs: a prospective study","volume":"27","author":"Shiha","year":"2020","journal-title":"J Viral Hepat"},{"key":"10.1016\/j.jgeb.2025.100583_b0060","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/j.jhep.2017.07.025","article-title":"Hepatocellular carcinoma risk following direct-acting antiviral HCV therapy: a systematic review, meta-analyses, and meta-regression","volume":"67","author":"Waziry","year":"2017","journal-title":"J Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0065","unstructured":"El-Zanaty F, Way A. Egypt Demographic and Health Survey 2008, 2009."},{"key":"10.1016\/j.jgeb.2025.100583_b0070","unstructured":"Ministry of Health and Population, E., El-Zanaty and Associates, E., ICF International, 2015. Egypt Health Issues Survey 2015."},{"key":"10.1016\/j.jgeb.2025.100583_b0075","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1056\/NEJMsr1912628","article-title":"Screening and treatment program to eliminate hepatitis C in Egypt","volume":"382","author":"Waked","year":"2020","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jgeb.2025.100583_b0080","unstructured":"WHO, 2021. Accelerating access to hepatitis C diagnostics and treatment [WWW Document]. URL https:\/\/www.who.int\/publications\/i\/item\/9789240019003 (accessed 11.1.24)."},{"key":"10.1016\/j.jgeb.2025.100583_b0085","doi-asserted-by":"crossref","first-page":"183","DOI":"10.2147\/JHC.S206668","article-title":"Hepatitis C antiviral therapy in patients with successfully treated hepatocellular carcinoma: dancing with wolves","volume":"6","author":"El Kassas","year":"2019","journal-title":"Journal of Hepatocellular Carcinoma"},{"key":"10.1016\/j.jgeb.2025.100583_b0090","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.gene.2019.02.080","article-title":"Detection of DNA methylated microRNAs in hepatocellular carcinoma","volume":"702","author":"Ezzat","year":"2019","journal-title":"Gene"},{"key":"10.1016\/j.jgeb.2025.100583_b0095","unstructured":"Globocan, 2024. Cancer Today [WWW Document]. Cancer TODAY | IARC - https:\/\/gco.iarc.who.int. URL https:\/\/gco.iarc.who.int\/today\/ (accessed 11.1.24)."},{"key":"10.1016\/j.jgeb.2025.100583_b0100","doi-asserted-by":"crossref","first-page":"19698","DOI":"10.3390\/ijms160819698","article-title":"Hepatocellular carcinoma in patients with a sustained response to anti-hepatitis C therapy","volume":"16","author":"D\u2019Ambrosio","year":"2015","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jgeb.2025.100583_b0105","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1515\/hsz-2017-0118","article-title":"Chronic viral hepatitis and its association with liver cancer","volume":"398","author":"Tu","year":"2017","journal-title":"Biol Chem"},{"key":"10.1016\/j.jgeb.2025.100583_b0110","doi-asserted-by":"crossref","first-page":"551","DOI":"10.3390\/jcm10030551","article-title":"Tracking down the epigenetic footprint of HCV-induced hepatocarcinogenesis","volume":"10","author":"Domovitz","year":"2021","journal-title":"J Clin Med"},{"key":"10.1016\/j.jgeb.2025.100583_b0115","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1053\/j.gastro.2019.02.038","article-title":"HCV-induced epigenetic changes associated with liver cancer risk persist after sustained virologic response","volume":"156","author":"Hamdane","year":"2019","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jgeb.2025.100583_b0120","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1008181","article-title":"Hepatitis C virus leaves an epigenetic signature post cure of infection by direct-acting antivirals","volume":"15","author":"Perez","year":"2019","journal-title":"PLoS Genet"},{"key":"10.1016\/j.jgeb.2025.100583_b0125","doi-asserted-by":"crossref","first-page":"12","DOI":"10.2119\/molmed.2010.00103","article-title":"High expression of H3K27me3 in human hepatocellular carcinomas correlates closely with vascular invasion and predicts worse prognosis in patients","volume":"17","author":"Cai","year":"2011","journal-title":"Mol Med"},{"key":"10.1016\/j.jgeb.2025.100583_b0130","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1002\/hep.29203","article-title":"Persisting risk of hepatocellular carcinoma after hepatitis C virus cure monitored by a liver transcriptome signature","volume":"66","author":"Ono","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.jgeb.2025.100583_b0135","doi-asserted-by":"crossref","first-page":"e416","DOI":"10.1038\/emm.2017.165","article-title":"The significance of intertumor and intratumor heterogeneity in liver cancer","volume":"50","author":"Liu","year":"2018","journal-title":"Exp Mol Med"},{"key":"10.1016\/j.jgeb.2025.100583_b0140","article-title":"The effect of lung cancer on cytokine expression in peripheral blood mononuclear cells","volume":"8","author":"Chang","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jgeb.2025.100583_b0145","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0056153","article-title":"Molecular signatures associated with HCV-induced hepatocellular carcinoma and liver metastasis","volume":"8","author":"De Giorgi","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jgeb.2025.100583_b0150","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/9541600","article-title":"Identifying potential new gene expression-based biomarkers in the peripheral blood mononuclear cells of hepatitis B-related hepatocellular carcinoma","volume":"2022","author":"Poortahmasebi","year":"2022","journal-title":"Can J Gastroenterol Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0155","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/s41586-020-2056-8","article-title":"Peripheral T cell expansion predicts tumour infiltration and clinical response","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jgeb.2025.100583_b0160","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/s12985-024-02551-3","article-title":"Peripheral immune signatures associated with the risk of hepatocarcinogenesis in cirrhotic Egyptian HCV patients before and after treatment with direct-acting antivirals","volume":"21","author":"El-Shenawy","year":"2024","journal-title":"Virol J"},{"key":"10.1016\/j.jgeb.2025.100583_b0165","doi-asserted-by":"crossref","first-page":"17797","DOI":"10.1038\/s41598-021-96952-x","article-title":"RNA-seq profiling reveals PBMC RNA as a potential biomarker for hepatocellular carcinoma","volume":"11","author":"Han","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.jgeb.2025.100583_b0170","doi-asserted-by":"crossref","first-page":"15735","DOI":"10.1038\/s41598-023-42560-w","article-title":"FAT4 expression in peripheral blood mononuclear cells is associated with prognosis and immune cell infiltration in hepatocellular carcinoma","volume":"13","author":"Li","year":"2023","journal-title":"Sci Rep"},{"key":"10.1016\/j.jgeb.2025.100583_b0175","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jgeb.2025.100583_b0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0294236","article-title":"SRplot: a free online platform for data visualization and graphing","volume":"18","author":"Tang","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.jgeb.2025.100583_b0185","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.jhep.2016.06.015","article-title":"Early occurrence and recurrence of hepatocellular carcinoma in HCV-related cirrhosis treated with direct-acting antivirals","volume":"65","author":"Conti","year":"2016","journal-title":"J Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0190","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.jhep.2016.04.008","article-title":"Unexpected high rate of early tumor recurrence in patients with HCV-related HCC undergoing interferon-free therapy","volume":"65","author":"Reig","year":"2016","journal-title":"J Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0195","first-page":"315","article-title":"Unraveling the role of the Wnt pathway in hepatocellular carcinoma: from molecular mechanisms to therapeutic implications","volume":"13","author":"Liang","year":"2025","journal-title":"J Clin Transl Hepatol"},{"key":"10.1016\/j.jgeb.2025.100583_b0200","article-title":"Wnt\/\u03b2-Catenin signaling pathway in hepatocellular carcinoma: pathogenic role and therapeutic target","volume":"14","author":"Zhao","year":"2024","journal-title":"Front Oncol"},{"key":"10.1016\/j.jgeb.2025.100583_b0205","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12985-014-0236-6","article-title":"Differential regulation of cytotoxicity pathway discriminating between HIV, HCV mono- and co-infection identified by transcriptome profiling of PBMCs","volume":"12","author":"Wu","year":"2015","journal-title":"Virol J"},{"key":"10.1016\/j.jgeb.2025.100583_b0210","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2023.1222265","article-title":"JunB: a paradigm for Jun family in immune response and cancer","volume":"13","author":"Ren","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.jgeb.2025.100583_b0215","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1038\/nrc1209","article-title":"AP-1: a double-edged sword in tumorigenesis","volume":"3","author":"Eferl","year":"2003","journal-title":"Nat Rev Cancer"},{"key":"10.1016\/j.jgeb.2025.100583_b0220","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1055\/s-0043-1768957","article-title":"Shared genes and molecular mechanisms between nonalcoholic fatty liver disease and hepatocellular carcinoma established by WGCNA analysis","volume":"10","author":"He","year":"2023","journal-title":"Glob Med Genet"},{"key":"10.1016\/j.jgeb.2025.100583_b0225","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s10585-011-9449-x","article-title":"Regulation of HGF-mediated cell proliferation and invasion through NF-\u03baB, JunB, and MMP-9 cascades in stomach cancer cells","volume":"29","author":"Lee","year":"2012","journal-title":"Clin Exp Metastasis"},{"key":"10.1016\/j.jgeb.2025.100583_b0230","first-page":"1478","article-title":"Tracking the important role of JUNB in hepatocellular carcinoma by single-cell sequencing analysis","volume":"19","author":"Yan","year":"2020","journal-title":"Oncol Lett"},{"key":"10.1016\/j.jgeb.2025.100583_b0235","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2021.107624","article-title":"Biodegradable Hypocrellin B nanoparticles coated with neutrophil membranes for hepatocellular carcinoma photodynamics therapy effectively via JUNB\/ROS signaling","volume":"99","author":"Zhang","year":"2021","journal-title":"Int Immunopharmacol"},{"key":"10.1016\/j.jgeb.2025.100583_b0240","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.cmet.2021.04.001","article-title":"Tumors exploit FTO-mediated regulation of glycolytic metabolism to evade immune surveillance","volume":"33","author":"Liu","year":"2021","journal-title":"Cell Metab"},{"key":"10.1016\/j.jgeb.2025.100583_b0245","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.3390\/ijms24021407","article-title":"Hepatitis C virus infection upregulates plasma phosphosphingolipids and endocannabinoids and downregulates lysophosphoinositols","volume":"24","author":"Beyo\u011flu","year":"2023","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jgeb.2025.100583_b0250","doi-asserted-by":"crossref","first-page":"922","DOI":"10.3390\/ijms17060922","article-title":"Persistence of HCV in acutely-infected patients depletes C24-ceramide and upregulates sphingosine and sphinganine serum levels","volume":"17","author":"Grammatikos","year":"2016","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jgeb.2025.100583_b0255","doi-asserted-by":"crossref","first-page":"63324","DOI":"10.18632\/oncotarget.11450","article-title":"Sphingosine-1-phosphate induced epithelial-mesenchymal transition of hepatocellular carcinoma via an MMP-7\/syndecan-1\/TGF-\u03b2 autocrine loop","volume":"7","author":"Zeng","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.jgeb.2025.100583_b0260","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.3390\/biomedicines9101446","article-title":"The persistence of hepatitis C virus infection in hepatocytes promotes hepatocellular carcinoma progression by pro-inflammatory interluekin-8 expression","volume":"9","author":"Shabangu","year":"2021","journal-title":"Biomedicines"},{"key":"10.1016\/j.jgeb.2025.100583_b0265","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1582-4934.2006.tb00292.x","article-title":"Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-beta as major players and therapeutic targets","volume":"10","author":"Gressner","year":"2006","journal-title":"J Cell Mol Med"},{"key":"10.1016\/j.jgeb.2025.100583_b0270","article-title":"Overexpression of endothelin 1 triggers hepatocarcinogenesis in zebrafish and promotes cell proliferation and migration through the AKT pathway","volume":"9","author":"Lu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jgeb.2025.100583_b0275","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1161\/01.HYP.32.1.89","article-title":"Stimulatory interaction between vascular endothelial growth factor and endothelin-1 on each gene expression","volume":"32","author":"Matsuura","year":"1998","journal-title":"Hypertension"},{"key":"10.1016\/j.jgeb.2025.100583_b0280","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1158\/1078-0432.CCR-15-0528","article-title":"Kr\u00fcppel-like factor 4 blocks hepatocellular carcinoma dedifferentiation and progression through activation of hepatocyte nuclear factor-6","volume":"22","author":"Sun","year":"2016","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.jgeb.2025.100583_b0285","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.1158\/0008-5472.CAN-04-3619","article-title":"Drastic down-regulation of kr\u00fcppel-like factor 4 expression is critical in human gastric cancer development and progression","volume":"65","author":"Wei","year":"2005","journal-title":"Cancer Res"},{"key":"10.1016\/j.jgeb.2025.100583_b0290","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S0022-2836(02)01449-3","article-title":"Transcriptional profiling of Kr\u00fcppel-like factor 4 reveals a function in cell cycle regulation and epithelial differentiation","volume":"326","author":"Chen","year":"2003","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jgeb.2025.100583_b0295","doi-asserted-by":"crossref","first-page":"16854","DOI":"10.1074\/jbc.M110.114546","article-title":"Kr\u00fcppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression *","volume":"285","author":"Yori","year":"2010","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jgeb.2025.100583_b0300","doi-asserted-by":"crossref","first-page":"953","DOI":"10.7150\/ijbs.30857","article-title":"KLF4-mediated CDH3 upregulation suppresses human hepatoma cell growth and migration via GSK-3\u03b2 signaling","volume":"15","author":"Li","year":"2019","journal-title":"Int J Biol Sci"},{"key":"10.1016\/j.jgeb.2025.100583_b0305","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.1111\/liv.15412","article-title":"ABI2-mediated MEOX2\/KLF4-NANOG axis promotes liver cancer stem cell and drives tumour recurrence","volume":"42","author":"Chen","year":"2022","journal-title":"Liver Int"},{"key":"10.1016\/j.jgeb.2025.100583_b0310","doi-asserted-by":"crossref","first-page":"3972","DOI":"10.3892\/mmr.2015.3848","article-title":"Effects of small interfering RNA-mediated downregulation of the Kr\u00fcppel-like factor 4 gene on collagen metabolism in human hepatic stellate cells","volume":"12","author":"Li","year":"2015","journal-title":"Mol Med Rep"},{"key":"10.1016\/j.jgeb.2025.100583_b0315","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1038\/onc.2016.447","article-title":"Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-\u03b2 signaling in and promote progression of hepatocellular carcinoma","volume":"36","author":"Sun","year":"2017","journal-title":"Oncogene"},{"key":"10.1016\/j.jgeb.2025.100583_b0320","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.gde.2009.12.002","article-title":"Circulating tumor cells: a window into cancer biology and metastasis","volume":"20","author":"Maheswaran","year":"2010","journal-title":"Curr Opin Genet Dev"},{"key":"10.1016\/j.jgeb.2025.100583_b0325","doi-asserted-by":"crossref","first-page":"14838","DOI":"10.1038\/s41598-021-94330-1","article-title":"The expression of FLNA and CLU in PBMCs as a novel screening marker for hepatocellular carcinoma","volume":"11","author":"Patarat","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.jgeb.2025.100583_b0330","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/S0140-6736(18)30010-2","article-title":"Hepatocellular carcinoma","volume":"391","author":"Forner","year":"2018","journal-title":"Lancet"},{"issue":"Suppl 1","key":"10.1016\/j.jgeb.2025.100583_b0335","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/hep.31175","article-title":"Biomarkers for hepatobiliary cancers","volume":"73","author":"Nault","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.jgeb.2025.100583_b0340","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s43066-024-00317-8","article-title":"Impact of treating chronic hepatitis C with direct acting antivirals on health-related quality of life: a real-life Egyptian experience","volume":"14","author":"Elbadry","year":"2024","journal-title":"Egyptian Liver Journal"},{"key":"10.1016\/j.jgeb.2025.100583_b0345","first-page":"483","article-title":"High-throughput Sequencing for differentially expressed microRNAs in Hepatocellular Carcinoma","volume":"68","author":"Eltaweel","year":"2025","journal-title":"Egypt J Chem"},{"key":"10.1016\/j.jgeb.2025.100583_b0350","doi-asserted-by":"crossref","first-page":"216","DOI":"10.3748\/wjg.v28.i2.216","article-title":"Update on the applications and limitations of alpha-fetoprotein for hepatocellular carcinoma","volume":"28","author":"Hanif","year":"2022","journal-title":"World J Gastroenterol"},{"key":"10.1016\/j.jgeb.2025.100583_b0355","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12876-023-03099-2","article-title":"Evaluation of patients treated with direct-acting anti-viral therapy for chronic hepatitis C and their risk of hepatocellular carcinoma in Hong Kong","volume":"24","author":"Chow","year":"2024","journal-title":"BMC Gastroenterol"},{"key":"10.1016\/j.jgeb.2025.100583_b0360","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.3390\/v16121899","article-title":"Chronic hepatitis C infection treated with direct-acting antiviral agents and occurrence\/recurrence of hepatocellular carcinoma: does it still matter?","volume":"16","author":"Smirne","year":"2024","journal-title":"Viruses"}],"container-title":["Journal of Genetic Engineering and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1687157X25001271?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1687157X25001271?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:49:17Z","timestamp":1778824157000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1687157X25001271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":72,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S1687157X25001271"],"URL":"https:\/\/doi.org\/10.1016\/j.jgeb.2025.100583","relation":{},"ISSN":["1687-157X"],"issn-type":[{"value":"1687-157X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessment of peripheral gene expression signatures as predictive biomarkers for hepatocellular carcinoma following DAA treatment","name":"articletitle","label":"Article Title"},{"value":"Journal of Genetic Engineering and Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgeb.2025.100583","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc. on behalf of Academy of Scientific Research and Technology.","name":"copyright","label":"Copyright"}],"article-number":"100583"}}