{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:10:05Z","timestamp":1789560605277,"version":"build-2803163510"},"reference-count":46,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Genetic Engineering and Biotechnology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jgeb.2026.100780","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:06:28Z","timestamp":1785294388000},"page":"100780","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering"],"prefix":"10.1016","volume":"24","author":[{"given":"Xiangxiang","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongdao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgeb.2026.100780_bb0005","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2180-5-59","article-title":"Polyamine stress at high pH in Escherichia coli K-12","author":"Yohannes","year":"2005","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0010","doi-asserted-by":"crossref","DOI":"10.1134\/S0026261709010044","article-title":"Adaptive functions of Escherichia coli polyamines in response to sublethal concentrations of antibiotics","author":"Tkachenko","year":"2009","journal-title":"Microbiology"},{"issue":"4","key":"10.1016\/j.jgeb.2026.100780_bb0015","first-page":"487","article-title":"Putrescine as a oxidative stress protecting factor in Escherichia coli","volume":"70","author":"Tkachenko","year":"2001","journal-title":"Mikrobiologiia"},{"key":"10.1016\/j.jgeb.2026.100780_bb0020","article-title":"Putrescine regulating by stress-responsive MAPK cascade contributes to bacterial pathogen defense in Arabidopsis","author":"Kim","year":"2013","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jgeb.2026.100780_bb0025","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2627990100","article-title":"Polyamines protect Escherichia coli cells from the toxic effect of oxygen","author":"Chattopadhyay","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jgeb.2026.100780_bb0030","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-007-0932-x","article-title":"Biomass in the manufacture of industrial products--the use of proteins and amino acids","author":"Scott","year":"2007","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0035","doi-asserted-by":"crossref","DOI":"10.1002\/mabi.200600223","article-title":"Bio-refinery as the bio-inspired process to bulk chemicals","author":"Sanders","year":"2007","journal-title":"Macromol Biosci"},{"key":"10.1016\/j.jgeb.2026.100780_bb0040","doi-asserted-by":"crossref","DOI":"10.1021\/acssuschemeng.3c05247","article-title":"Genetically encoded multienzyme particles for the biosynthesis of putrescine from L-arginine","author":"Xiong","year":"2024","journal-title":"ACS Sustain Chem Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0045","article-title":"Highly efficient bio-production of putrescine from L-arginine with arginase and L-ornithine decarboxylase in engineered Escherichia coli","author":"Wang","year":"2024","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0050","doi-asserted-by":"crossref","DOI":"10.1021\/acssuschemeng.2c02602","article-title":"Production of putrescine in metabolic engineering Corynebacterium crenatum by mixed sugar fermentation","author":"Yang","year":"2022","journal-title":"ACS Sustain Chem Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0055","article-title":"Rationally engineering pH adaptation of acid-induced arginine decarboxylase from Escherichia coli to alkaline environments to efficiently biosynthesize putrescine","author":"Wang","year":"2024","journal-title":"Advanced science (Weinheim, Baden-Wurttemberg, Germany)"},{"key":"10.1016\/j.jgeb.2026.100780_bb0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.synbio.2026.02.008","article-title":"De novo biosynthesis of putrescine from glucose using engineered Escherichia coli in a two-step process","author":"Yin","year":"2026","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymben.2026.05.005","article-title":"Metabolic rewiring and biosensor-assisted screening for high-level putrescine production from xylose in Escherichia coli","author":"Han","year":"2026","journal-title":"Metab Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0070","article-title":"Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater","author":"Nor","year":"2022","journal-title":"Bioprocess Biosyst Eng"},{"issue":"13","key":"10.1016\/j.jgeb.2026.100780_bb0075","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1016\/S0021-9258(18)96507-5","article-title":"Multiple pathways of putrescine biosynthesis in Escherichia coli","volume":"241","author":"Morris","year":"1966","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jgeb.2026.100780_bb0080","doi-asserted-by":"crossref","DOI":"10.1016\/0006-291X(65)90072-0","article-title":"A biosynthetic ornithine decarboxylase in Escherichia coli","author":"Morris","year":"1965","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jgeb.2026.100780_bb0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.nbt.2025.07.001","article-title":"Model-based engineering of bacillus methanolicus towards de novo polyamine bioproduction from methanol","author":"Brito","year":"2025","journal-title":"N Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2013.02.053","article-title":"Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum","author":"Meiswinkel","year":"2013","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0095","doi-asserted-by":"crossref","DOI":"10.1002\/bit.22502","article-title":"Metabolic engineering of Escherichia coli for the production of putrescine: a four carbon diamine","author":"Qian","year":"2009","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/metabo5020211","article-title":"Fermentative production of the diamine putrescine: system metabolic engineering of Corynebacterium glutamicum","author":"Nguyen","year":"2015","journal-title":"Metabolites"},{"key":"10.1016\/j.jgeb.2026.100780_bb0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.cels.2017.08.016","article-title":"Gene expression knockdown by modulating synthetic small RNA expression in Escherichia coli","author":"Noh","year":"2017","journal-title":"Cell Syst"},{"key":"10.1016\/j.jgeb.2026.100780_bb0110","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.635509","article-title":"Production of biopolyamide precursors 5-amino Valeric acid and putrescine from Rice straw hydrolysate by engineered Corynebacterium glutamicum","author":"Sasikumar","year":"2021","journal-title":"Front Bioeng Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0115","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-012-3956-9","article-title":"Improving putrescine production by Corynebacterium glutamicum by fine-tuning ornithine transcarbamoylase activity using a plasmid addiction system","author":"Schneider","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymben.2026.01.006","article-title":"Metabolic engineering of Escherichia coli for the high-level production of putrescine","author":"Ko","year":"2026","journal-title":"Metab Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0125","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1006138107","article-title":"Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control","author":"Leonard","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jgeb.2026.100780_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2013.02.001","article-title":"Microbial engineering strategies to improve cell viability for biochemical production","author":"Lo","year":"2013","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.jgeb.2026.100780_bb0135","doi-asserted-by":"crossref","DOI":"10.1038\/nbt833","article-title":"Engineering a mevalonate pathway in Escherichia coli for production of terpenoids","author":"Martin","year":"2003","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0140","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201100086","article-title":"The zero-sum game of pathway optimization: emerging paradigms for tuning gene expression","author":"Solomon","year":"2011","journal-title":"Biotechnol J"},{"key":"10.1016\/j.jgeb.2026.100780_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymben.2012.02.001","article-title":"The future of metabolic engineering and synthetic biology: towards a systematic practice","author":"Yadav","year":"2012","journal-title":"Metab Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0150","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-chembioeng-061312-103312","article-title":"Metabolic engineering: past and future","author":"Woolston","year":"2013","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.copbio.2014.05.005","article-title":"Multivariate modular metabolic engineering for pathway and strain optimization","author":"Biggs","year":"2014","journal-title":"Curr Opin Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbiotec.2013.07.030","article-title":"Multivariate modular metabolic engineering of Escherichia coli to produce resveratrol from L-tyrosine","author":"Wu","year":"2013","journal-title":"J Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0165","doi-asserted-by":"crossref","DOI":"10.1186\/s13068-016-0675-y","article-title":"Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production","author":"Tan","year":"2016","journal-title":"Biotechnol Biofuels"},{"key":"10.1016\/j.jgeb.2026.100780_bb0170","doi-asserted-by":"crossref","DOI":"10.1021\/sb300016b","article-title":"ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. Coli","author":"Xu","year":"2012","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0175","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-006-0792-9","article-title":"L-tyrosine production by deregulated strains of Escherichia coli","author":"L\u00fctke-Eversloh","year":"2007","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0180","doi-asserted-by":"crossref","DOI":"10.1038\/srep28629","article-title":"Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production","author":"Man","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.jgeb.2026.100780_bb0185","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-014-0166-4","article-title":"Metabolic engineering of microorganisms for the production of L-arginine and its derivatives","author":"Shin","year":"2014","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.jgeb.2026.100780_bb0190","article-title":"Metabolic engineering of Escherichia coli for agmatine production","author":"Xu","year":"2018","journal-title":"Eng Life Sci"},{"key":"10.1016\/j.jgeb.2026.100780_bb0195","doi-asserted-by":"crossref","DOI":"10.1186\/s13568-021-01330-5","article-title":"Improvement of putrescine production through the arginine decarboxylase pathway in Escherichia coli K-12","author":"Thongbhubate","year":"2021","journal-title":"AMB Express"},{"key":"10.1016\/j.jgeb.2026.100780_bb0200","doi-asserted-by":"crossref","DOI":"10.1002\/bit.27456","article-title":"Pathway engineering of Escherichia coli for \u03b1-ketoglutaric acid production","author":"Chen","year":"2020","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.jgeb.2026.100780_bb0205","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00104-08","article-title":"Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12","author":"Kim","year":"2008","journal-title":"J Bacteriol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0210","doi-asserted-by":"crossref","DOI":"10.3390\/metabo10040166","article-title":"Metabolic engineering design strategies for increasing acetyl-CoA flux","author":"Ku","year":"2020","journal-title":"Metabolites"},{"key":"10.1016\/j.jgeb.2026.100780_bb0215","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-010-2778-x","article-title":"Putrescine production by engineered Corynebacterium glutamicum","author":"Schneider","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0220","doi-asserted-by":"crossref","DOI":"10.1128\/JB.01295-10","article-title":"Corynebacterium glutamicum as a host for synthesis and export of D-amino acids","author":"St\u00e4bler","year":"2011","journal-title":"J Bacteriol"},{"key":"10.1016\/j.jgeb.2026.100780_bb0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.jiac.2022.07.022","article-title":"First description of a clinical glutamine-dependent Escherichia coli with a missense mutation in the glnA","author":"Matsumoto","year":"2022","journal-title":"J Infect Chemother"},{"key":"10.1016\/j.jgeb.2026.100780_bb0230","article-title":"Improvement of L-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114","author":"Zhang","year":"2018","journal-title":"AMB Express"}],"container-title":["Journal of Genetic Engineering and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1687157X26001241?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1687157X26001241?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T11:48:55Z","timestamp":1789559335000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1687157X26001241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S1687157X26001241"],"URL":"https:\/\/doi.org\/10.1016\/j.jgeb.2026.100780","relation":{},"ISSN":["1687-157X"],"issn-type":[{"value":"1687-157X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering","name":"articletitle","label":"Article Title"},{"value":"Journal of Genetic Engineering and Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgeb.2026.100780","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Academy of Scientific Research and Technology.","name":"copyright","label":"Copyright"}],"article-number":"100780"}}