{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:07:07Z","timestamp":1790842027077,"version":"4.1.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["9184910039"],"award-info":[{"award-number":["9184910039"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31272711"],"award-info":[{"award-number":["31272711"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31330034"],"award-info":[{"award-number":["31330034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["3207090129"],"award-info":[{"award-number":["3207090129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Genetics and Genomics"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.jgg.2021.04.014","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T13:08:57Z","timestamp":1623244137000},"page":"452-462","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["LIMK2 is required for membrane cytoskeleton reorganization of contracting airway smooth muscle"],"prefix":"10.1016","volume":"48","author":[{"given":"Yeqiong","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisha","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Na","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3256-6861","authenticated-orcid":false,"given":"Min-Sheng","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgg.2021.04.014_bib1","doi-asserted-by":"crossref","first-page":"563a","DOI":"10.1016\/j.bpj.2019.11.3074","article-title":"Ano1, cav1.2 and ip3r form a functional unit of excitation-contraction coupling during agonist-mediated contraction of mouse pulmonary arterial smooth muscle","volume":"118","author":"Aoun","year":"2020","journal-title":"Biophys. J."},{"key":"10.1016\/j.jgg.2021.04.014_bib2","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1113\/jphysiol.2014.283853","article-title":"In vivo roles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction","volume":"593","author":"Chen","year":"2015","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib3","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1083\/jcb.111.6.2463","article-title":"The cytoskeletal and contractile apparatus of smooth muscle: contraction bands and segmentation of the contractile elements","volume":"111","author":"Draeger","year":"1990","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.jgg.2021.04.014_bib4","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1152\/physrev.1984.64.2.455","article-title":"Structural apparatus for force transmission in smooth muscles","volume":"64","author":"Gabella","year":"1984","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.jgg.2021.04.014_bib5","doi-asserted-by":"crossref","first-page":"C1267","DOI":"10.1152\/ajpcell.1995.268.5.C1267","article-title":"Mechanisms for the mechanical plasticity of tracheal smooth muscle","volume":"268","author":"Gunst","year":"1995","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib6","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.1152\/jappl.2001.91.6.2511","article-title":"Bronchoprotective and bronchodilatory effects of deep inspiration in rabbits subjected to bronchial challenge","volume":"91","author":"Gunst","year":"2001","journal-title":"J.\u00a0Appl. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib7","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1152\/jappl.1990.68.5.2223","article-title":"Mechanical modulation of pressure-volume characteristics of contracted canine airways in\u00a0vitro","volume":"68","author":"Gunst","year":"1990","journal-title":"J.\u00a0Appl. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib8","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1034\/j.1399-3003.2000.15.29.x","article-title":"The contractile apparatus and mechanical properties of airway smooth muscle","volume":"15","author":"Gunst","year":"2000","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.jgg.2021.04.014_bib9","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S1569-9048(03)00144-7","article-title":"Cytoskeletal remodeling of the airway smooth muscle cell: a mechanism for adaptation to mechanical forces in the lung","volume":"137","author":"Gunst","year":"2003","journal-title":"Respir. Physiol. Neurobiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib10","doi-asserted-by":"crossref","first-page":"C576","DOI":"10.1152\/ajpcell.00253.2008","article-title":"Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction","volume":"295","author":"Gunst","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib11","doi-asserted-by":"crossref","first-page":"H584","DOI":"10.1152\/ajpheart.01212.2010","article-title":"Role of myosin light chain kinase in regulation of basal blood pressure and maintenance of salt-induced hypertension","volume":"301","author":"He","year":"2011","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib12","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1053\/j.gastro.2013.02.045","article-title":"Altered contractile phenotypes of intestinal smooth muscle in mice deficient in myosin phosphatase target subunit 1","volume":"144","author":"He","year":"2013","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jgg.2021.04.014_bib13","doi-asserted-by":"crossref","first-page":"L911","DOI":"10.1152\/ajplung.1998.275.5.L911","article-title":"G\u03b1i-2 is required for carbachol-induced stress fiber formation in human airway smooth muscle cells","volume":"275","author":"Hirshman","year":"1998","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0042601","article-title":"Inhibition of p21 activated kinase (pak) reduces airway responsiveness in vivo and in vitro in murine and human airways","volume":"7","author":"Hoover","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jgg.2021.04.014_bib15","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1152\/physrev.1996.76.4.967","article-title":"Mechanisms of smooth muscle contraction","volume":"76","author":"Horowitz","year":"1996","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.jgg.2021.04.014_bib16","doi-asserted-by":"crossref","first-page":"3630","DOI":"10.1074\/jbc.M110.139923","article-title":"Activation of vinculin induced by cholinergic stimulation regulates contraction of tracheal smooth muscle tissue","volume":"286","author":"Huang","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jgg.2021.04.014_bib17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1113\/jphysiol.2014.275107","article-title":"Activation of Ca2+ -activated Cl- channel ano1 by localized Ca2+ signals","volume":"594","author":"Jin","year":"2016","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib18","doi-asserted-by":"crossref","first-page":"ra73","DOI":"10.1126\/scisignal.2004184","article-title":"Activation of the Cl- channel ANO1 by localized calcium signals in nociceptive sensory neurons requires coupling with the IP3 receptor","volume":"6","author":"Jin","year":"2013","journal-title":"Sci. Signal."},{"key":"10.1016\/j.jgg.2021.04.014_bib19","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.pa.25.040185.003113","article-title":"The function of myosin and myosin light chain kinase phosphorylation in smooth muscle","volume":"25","author":"Kamm","year":"1985","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.jgg.2021.04.014_bib20","first-page":"681.611","article-title":"Role of voltage-dependent calcium channels in stretch-induced lymphatic vessel contractions","volume":"27","author":"Lee","year":"2013","journal-title":"Faseb. J."},{"key":"10.1016\/j.jgg.2021.04.014_bib21","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1002\/med.20230","article-title":"Lim kinases are attractive targets with many macromolecular partners and only a few small molecule regulators","volume":"32","author":"Manetti","year":"2012","journal-title":"Med. Res. Rev."},{"issue":"Pt 3","key":"10.1016\/j.jgg.2021.04.014_bib22","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1111\/j.1469-7793.1999.0829n.x","article-title":"Actin polymerization stimulated by contractile activation regulates force development in canine tracheal smooth muscle","volume":"519","author":"Mehta","year":"1999","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib23","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.cellsig.2012.11.001","article-title":"Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation","volume":"25","author":"Mizuno","year":"2013","journal-title":"Cell Signal."},{"key":"10.1016\/j.jgg.2021.04.014_bib24","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1152\/jappl.1961.16.4.717","article-title":"Effect of a previous deep inspiration on airway resistance in man","volume":"16","author":"Nadel","year":"1961","journal-title":"J.\u00a0Appl. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib25","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0014-2999(94)90092-2","article-title":"Effect of cytochalasin b on intestinal smooth muscle cells","volume":"255","author":"Obara","year":"1994","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.jgg.2021.04.014_bib26","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/dgd.12213","article-title":"Roles of cofilin in development and its mechanisms of regulation","volume":"57","author":"Ohashi","year":"2015","journal-title":"Dev. Growth Differ."},{"key":"10.1016\/j.jgg.2021.04.014_bib27","doi-asserted-by":"crossref","first-page":"S187","DOI":"10.1164\/ajrccm.158.supplement_2.13tac170","article-title":"Characteristics of airway hyperresponsiveness in asthma and chronic obstructive pulmonary disease","volume":"158","author":"Postma","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.jgg.2021.04.014_bib28","doi-asserted-by":"crossref","first-page":"L1113","DOI":"10.1152\/ajplung.00370.2016","article-title":"Emerging concepts in smooth muscle contributions to airway structure and function: implications for health and disease","volume":"311","author":"Prakash","year":"2016","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib29","doi-asserted-by":"crossref","first-page":"41749","DOI":"10.18632\/oncotarget.16978","article-title":"Lim kinases: cofilin and beyond","volume":"8","author":"Prunier","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.jgg.2021.04.014_bib30","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1016\/j.cell.2013.08.021","article-title":"Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity","volume":"154","author":"Ran","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.jgg.2021.04.014_bib31","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nmeth.1220","article-title":"Lifeact: a versatile marker to visualize f-actin","volume":"5","author":"Riedl","year":"2008","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jgg.2021.04.014_bib32","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1540\/jsmr.32.51","article-title":"Cytochalasin D inhibits smooth muscle contraction by directly inhibiting contractile apparatus","volume":"32","author":"Saito","year":"1996","journal-title":"J.\u00a0Smooth Muscle Res."},{"key":"10.1016\/j.jgg.2021.04.014_bib33","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1242\/jcs.045112","article-title":"Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions","volume":"123","author":"Schaller","year":"2010","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.jgg.2021.04.014_bib34","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00109-007-0165-6","article-title":"LIM kinases: function, regulation and association with human disease","volume":"85","author":"Scott","year":"2007","journal-title":"J.\u00a0Mol. Med."},{"key":"10.1016\/j.jgg.2021.04.014_bib35","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1152\/jappl.1997.83.4.1202","article-title":"Effect of tidal volume and frequency on airway responsiveness in mechanically ventilated rabbits","volume":"83","author":"Shen","year":"1997","journal-title":"J.\u00a0Appl. Physiol. (1985)"},{"key":"10.1016\/j.jgg.2021.04.014_bib36","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1152\/ajplegacy.1976.231.5.1501","article-title":"Calcium-dependent resistance to stretch and stress relaxation in resting smooth muscles","volume":"231","author":"Siegman","year":"1976","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib37","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1385\/CRIAI:24:1:55","article-title":"Bronchodilation and bronchoprotection by deep inspiration and their relationship to bronchial hyperresponsiveness","volume":"24","author":"Skloot","year":"2003","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"10.1016\/j.jgg.2021.04.014_bib38","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1002\/bies.950170908","article-title":"Structure-function relationships in smooth muscle: the missing links","volume":"17","author":"Small","year":"1995","journal-title":"Bioessays"},{"key":"10.1016\/j.jgg.2021.04.014_bib39","doi-asserted-by":"crossref","first-page":"C250","DOI":"10.1152\/ajpcell.1999.276.1.C250","article-title":"Mechanosensitive tyrosine phosphorylation of paxillin and focal adhesion kinase in tracheal smooth muscle","volume":"276","author":"Tang","year":"1999","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib40","doi-asserted-by":"crossref","first-page":"C874","DOI":"10.1152\/ajpcell.2001.280.4.C874","article-title":"Depletion of focal adhesion kinase by antisense depresses contractile activation of smooth muscle","volume":"280","author":"Tang","year":"2001","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.jgg.2021.04.014_bib41","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1053\/rmed.1999.0737","article-title":"Control of airway smooth muscle tone. I - electrophysiology and contractile mediators","volume":"94","author":"Thirstrup","year":"2000","journal-title":"Respir. Med."},{"key":"10.1016\/j.jgg.2021.04.014_bib42","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1016\/j.jaci.2017.05.053","article-title":"Inflammatory mediators mediate airway smooth muscle contraction through a G protein-coupled receptor-transmembrane protein 16A-voltage-dependent Ca2+ channel axis and contribute to bronchial hyperresponsiveness in asthma","volume":"141","author":"Wang","year":"2018","journal-title":"J.\u00a0Allergy Clin. Immunol."},{"key":"10.1016\/j.jgg.2021.04.014_bib43","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1183\/09031936.00204311","article-title":"The mechanism of deep inspiration-induced bronchoprotection: evidence from a mouse model","volume":"40","author":"Wong","year":"2012","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.jgg.2021.04.014_bib44","first-page":"213","article-title":"Establishment and pathological study of models of chronic obstructive pulmonary disease by SO2 inhalation method","volume":"113","author":"Xu","year":"2000","journal-title":"Chin. Med. J. (Engl)"},{"key":"10.1016\/j.jgg.2021.04.014_bib45","doi-asserted-by":"crossref","first-page":"5522","DOI":"10.1074\/jbc.M109.062836","article-title":"Myosin light chain kinase is necessary for tonic airway smooth muscle contraction","volume":"285","author":"Zhang","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jgg.2021.04.014_bib46","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1513\/pats.200704-048VS","article-title":"Interactions of airway smooth muscle cells with their tissue matrix: implications for contraction","volume":"5","author":"Zhang","year":"2008","journal-title":"Proc. Am. Thorac. Soc."},{"key":"10.1016\/j.jgg.2021.04.014_bib47","first-page":"0108051","article-title":"Molecular mechanisms for the mechanical modulation of airway responsiveness","volume":"2","author":"Zhang","year":"2019","journal-title":"J.\u00a0Eng. Sci. Med. Diagnost. Ther."},{"key":"10.1016\/j.jgg.2021.04.014_bib48","doi-asserted-by":"crossref","first-page":"36522","DOI":"10.1074\/jbc.M805294200","article-title":"Actin depolymerization factor\/cofilin activation regulates actin polymerization and tension development in canine tracheal smooth muscle","volume":"283","author":"Zhao","year":"2008","journal-title":"J.\u00a0Biol. Chem."}],"container-title":["Journal of Genetics and Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1673852721001533?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1673852721001533?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T21:40:59Z","timestamp":1778622059000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1673852721001533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["S1673852721001533"],"URL":"https:\/\/doi.org\/10.1016\/j.jgg.2021.04.014","relation":{},"ISSN":["1673-8527"],"issn-type":[{"value":"1673-8527","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"LIMK2 is required for membrane cytoskeleton reorganization of contracting airway smooth muscle","name":"articletitle","label":"Article Title"},{"value":"Journal of Genetics and Genomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgg.2021.04.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.","name":"copyright","label":"Copyright"}]}}