{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:13:21Z","timestamp":1784718801369,"version":"3.55.0"},"reference-count":67,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2601200"],"award-info":[{"award-number":["2022YFC2601200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0707900"],"award-info":[{"award-number":["2020YFA0707900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2604904"],"award-info":[{"award-number":["2023YFC2604904"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M743477"],"award-info":[{"award-number":["2023M743477"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32225030"],"award-info":[{"award-number":["32225030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82488301"],"award-info":[{"award-number":["82488301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000148","name":"Canadian Anesthesiologists' Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000148","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Genetics and Genomics"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.jgg.2025.10.007","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T01:51:28Z","timestamp":1762307488000},"page":"732-743","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dual-target CRISPR-Cas12 diagnostics based on asymmetrically chemical-modified DNA probe"],"prefix":"10.1016","volume":"53","author":[{"given":"Xinge","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangcan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqiu","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bangwei","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changxian","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongjian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiye","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyou","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgg.2025.10.007_bib1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2308224120","article-title":"Diversity, evolution, and classification of the RNA-guided nucleases TnpB and Cas12","volume":"120","author":"Altae-Tran","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jgg.2025.10.007_bib2","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1016\/S1473-3099(11)70241-9","article-title":"Prevalence and implications of multiple-strain infections","volume":"11","author":"Balmer","year":"2011","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jgg.2025.10.007_bib3","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41587-024-02255-7","article-title":"Trans-nuclease activity of Cas9 activated by DNA or RNA target binding","volume":"43","author":"Chen","year":"2024","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jgg.2025.10.007_bib4","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib5","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/s11427-022-2237-1","article-title":"Computer-aided engineering of CRISPR-Cas proteins for enhanced human genome editing","volume":"66","author":"Chen","year":"2022","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib6","article-title":"Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing","volume":"3","author":"Chen","year":"2022","journal-title":"Innovation"},{"key":"10.1016\/j.jgg.2025.10.007_bib7","first-page":"3","article-title":"Respiratory syncytial virus: virology, reverse genetics, and pathogenesis of disease","volume":"372","author":"Collins","year":"2013","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.jgg.2025.10.007_bib8","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib9","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1038\/s41564-019-0513-7","article-title":"The structure of the influenza A virus genome","volume":"4","author":"Dadonaite","year":"2019","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.jgg.2025.10.007_bib10","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nature19802","article-title":"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection","volume":"538","author":"East-Seletsky","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.jgg.2025.10.007_bib11","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.molcel.2019.09.013","article-title":"Programmable inhibition and detection of RNA viruses using Cas13","volume":"76","author":"Freije","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib12","doi-asserted-by":"crossref","first-page":"5512","DOI":"10.1038\/s41467-020-19344-1","article-title":"A catalogue of biochemically diverse CRISPR-Cas9 orthologs","volume":"11","author":"Gasiunas","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jgg.2025.10.007_bib13","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib14","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib15","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41421-020-0174-y","article-title":"SARS-CoV-2 detection with CRISPR diagnostics","volume":"6","author":"Guo","year":"2020","journal-title":"Cell Discov."},{"key":"10.1016\/j.jgg.2025.10.007_bib16","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.aav4294","article-title":"Programmed DNA destruction by miniature CRISPR-Cas14 enzymes","volume":"362","author":"Harrington","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib17","doi-asserted-by":"crossref","first-page":"29738","DOI":"10.1073\/pnas.2010783117","article-title":"Seasonal and diel patterns of abundance and activity of viruses in the red sea","volume":"117","author":"Hevroni","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jgg.2025.10.007_bib18","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.cell.2009.01.019","article-title":"The many pathways of RNA degradation","volume":"136","author":"Houseley","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib19","article-title":"Expanding Cas12a activity control with an RNA G-quadruplex at the 5\u2032 end of CRISPR RNA","volume":"12","author":"Huang","year":"2024","journal-title":"Adv. Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib20","doi-asserted-by":"crossref","first-page":"5241","DOI":"10.1038\/s41467-020-19072-6","article-title":"Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2","volume":"11","author":"Huang","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jgg.2025.10.007_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-10211-x","article-title":"Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR","volume":"15","author":"Huber","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jgg.2025.10.007_bib22","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib23","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1056\/NEJMc2026172","article-title":"Detection of SARS-CoV-2 with SHERLOCK one-pot testing","volume":"383","author":"Joung","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jgg.2025.10.007_bib24","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.jgg.2025.10.007_bib25","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1136\/jclinpath-2013-201661","article-title":"Internal quality control: best practice","volume":"66","author":"Kinns","year":"2013","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.jgg.2025.10.007_bib26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-018-0011-0","article-title":"Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing","volume":"37","author":"Kleinstiver","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jgg.2025.10.007_bib27","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.1021\/acs.biochem.3c00159","article-title":"Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox","volume":"62","author":"Koonin","year":"2023","journal-title":"Biochemistry"},{"key":"10.1016\/j.jgg.2025.10.007_bib28","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1021\/acsabm.1c01320","article-title":"Microfluidics-based point-of-care testing (POCT) devices in dealing with waves of COVID-19 pandemic: the emerging solution","volume":"5","author":"Kumar","year":"2022","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.jgg.2025.10.007_bib29","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1007\/s11427-021-2057-0","article-title":"Gene editing and its applications in biomedicine","volume":"65","author":"Li","year":"2022","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib30","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1007\/s11427-021-2028-x","article-title":"A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13","volume":"65","author":"Li","year":"2021","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib31","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.molcel.2016.11.040","article-title":"C2c1-sgRNA complex structure reveals RNA-guided DNA cleavage mechanism","volume":"65","author":"Liu","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib32","article-title":"Quality assessment of RNA in long-term storage: the all our families biorepository","volume":"15","author":"Luo","year":"2020","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jgg.2025.10.007_bib33","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1038\/s41467-022-30465-7","article-title":"Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editing","volume":"13","author":"McGaw","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jgg.2025.10.007_bib34","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.cccn.2005.05.050","article-title":"Nucleic acid-based methods for the detection of bacterial pathogens: present and future considerations for the clinical laboratory","volume":"363","author":"Mothershed","year":"2006","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.jgg.2025.10.007_bib35","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable viral diagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib36","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol. Cell Probes"},{"key":"10.1016\/j.jgg.2025.10.007_bib37","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jgg.2025.10.007_bib38","first-page":"1281","article-title":"Current trends in the management and prevention of human papillomavirus (HPV) infection","volume":"25","author":"Ntanasis-Stathopoulos","year":"2020","journal-title":"J. BUON."},{"key":"10.1016\/j.jgg.2025.10.007_bib39","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/pro.3943","article-title":"UCSF ChimeraX: structure visualization for researchers, educators, and developers","volume":"30","author":"Pettersen","year":"2020","journal-title":"Protein Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"key":"10.1016\/j.jgg.2025.10.007_bib41","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s41664-022-00212-4","article-title":"Improved strategies for CRISPR-Cas12-based nucleic acids detection","volume":"6","author":"Qiu","year":"2022","journal-title":"J. Anal. Test."},{"key":"10.1016\/j.jgg.2025.10.007_bib42","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s11427-023-2407-0","article-title":"Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12i(Max)","volume":"67","author":"Ren","year":"2024","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib43","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2022.866121","article-title":"New hope for genome editing in cultivated grasses: CRISPR variants and application","volume":"13","author":"Riaz","year":"2022","journal-title":"Front. Genet."},{"key":"10.1016\/j.jgg.2025.10.007_bib44","doi-asserted-by":"crossref","first-page":"9275","DOI":"10.1093\/nar\/gkr606","article-title":"The Streptococcus thermophilus CRISPR\/Cas system provides immunity in Escherichia coli","volume":"39","author":"Sapranauskas","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jgg.2025.10.007_bib45","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1007\/s11033-023-08239-1","article-title":"An update on CRISPR-Cas12 as a versatile tool in genome editing","volume":"50","author":"Senthilnathan","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.jgg.2025.10.007_bib46","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1093\/nar\/21.11.2605","article-title":"Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages","volume":"21","author":"Shimayama","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jgg.2025.10.007_bib47","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.molcel.2015.10.008","article-title":"Discovery and functional characterization of diverse class 2 CRISPR-Cas systems","volume":"60","author":"Shmakov","year":"2015","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib48","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1080\/1040841X.2021.2025041","article-title":"The era of Cas12 and Cas13 CRISPR-based disease diagnosis","volume":"48","author":"Singh","year":"2022","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.jgg.2025.10.007_bib49","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1752-8062.2010.00257.x","article-title":"Impact of pollution, climate, and sociodemographic factors on spatiotemporal dynamics of seasonal respiratory viruses","volume":"4","author":"Sloan","year":"2011","journal-title":"Clin. Transl. Sci."},{"key":"10.1016\/j.jgg.2025.10.007_bib50","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1089\/hum.2023.201","article-title":"Knockout and replacement gene surgery to treat rhodopsin-mediated autosomal dominant retinitis pigmentosa","volume":"35","author":"Sun","year":"2024","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.jgg.2025.10.007_bib51","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.molcel.2018.11.021","article-title":"Mechanistic insights into the cis- and trans-acting DNase activities of Cas12a","volume":"73","author":"Swarts","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib52","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1021\/acschembio.4c00247","article-title":"Miniature CRISPR-Cas12 systems: mechanisms, engineering, and genome editing applications","volume":"19","author":"Tang","year":"2024","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.jgg.2025.10.007_bib53","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/s41421-018-0069-3","article-title":"Repurposing CRISPR-Cas12b for mammalian genome engineering","volume":"4","author":"Teng","year":"2018","journal-title":"Cell Discov."},{"key":"10.1016\/j.jgg.2025.10.007_bib54","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1186\/s13059-019-1742-z","article-title":"CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity","volume":"20","author":"Teng","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.jgg.2025.10.007_bib55","article-title":"The versatile type V CRISPR effectors and their application prospects","volume":"8","author":"Tong","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.jgg.2025.10.007_bib56","doi-asserted-by":"crossref","first-page":"6392","DOI":"10.1038\/s41467-025-61748-4","article-title":"Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b","volume":"16","author":"Wang","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jgg.2025.10.007_bib57","article-title":"Enhancement of CRISPR-Cas12a system through universal circular RNA design","volume":"5","author":"Wang","year":"2025","journal-title":"Cell Rep. Methods"},{"key":"10.1016\/j.jgg.2025.10.007_bib58","article-title":"CDetection.v2: one-pot assay for the detection of SARS-CoV-2","volume":"14","author":"Wang","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jgg.2025.10.007_bib59","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202214987","article-title":"CRISPR-Cas biochemistry and CRISPR-based molecular diagnostics","volume":"62","author":"Weng","year":"2023","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.jgg.2025.10.007_bib60","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.2537","article-title":"Human papillomavirus infection: epidemiology, biology, host interactions, cancer development, prevention, and therapeutics","volume":"34","author":"Wolf","year":"2024","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.jgg.2025.10.007_bib61","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.molcel.2017.06.035","article-title":"Structural basis for the canonical and non-canonical PAM recognition by CRISPR-Cpf1","volume":"67","author":"Yamano","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib62","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1126\/science.aav7271","article-title":"Functionally diverse type V CRISPR-Cas systems","volume":"363","author":"Yan","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.jgg.2025.10.007_bib63","article-title":"Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview","volume":"15","author":"Yang","year":"2024","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jgg.2025.10.007_bib64","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.1007\/s11033-023-08240-8","article-title":"Research progress on nucleic acid detection and genome editing of CRISPR\/Cas12 system","volume":"50","author":"Yang","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.jgg.2025.10.007_bib65","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.cell.2015.09.038","article-title":"Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system","volume":"163","author":"Zetsche","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib66","first-page":"538","article-title":"An engineered xCas12i with high activity, high specificity and broad PAM range","volume":"14","author":"Zhang","year":"2022","journal-title":"Protein Cell"},{"key":"10.1016\/j.jgg.2025.10.007_bib67","article-title":"Intrinsic RNA targeting triggers indiscriminate DNase activity of CRISPR-Cas12a","volume":"63","author":"Zhang","year":"2024","journal-title":"Angew. Chem. Int. Ed."}],"container-title":["Journal of Genetics and Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1673852725002863?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1673852725002863?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:09:36Z","timestamp":1777043376000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1673852725002863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":67,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S1673852725002863"],"URL":"https:\/\/doi.org\/10.1016\/j.jgg.2025.10.007","relation":{},"ISSN":["1673-8527"],"issn-type":[{"value":"1673-8527","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual-target CRISPR-Cas12 diagnostics based on asymmetrically chemical-modified DNA probe","name":"articletitle","label":"Article Title"},{"value":"Journal of Genetics and Genomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgg.2025.10.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}