{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:22:12Z","timestamp":1782271332926,"version":"3.54.5"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["jhep-reports.eu","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["JHEP Reports"],"published-print":{"date-parts":[[2025,2]]},"DOI":"10.1016\/j.jhepr.2024.101252","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T17:24:34Z","timestamp":1730136274000},"page":"101252","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response"],"prefix":"10.1016","volume":"7","author":[{"given":"Emilie","family":"Crouchet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Durand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Parnot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine A.","family":"Oudot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Giannone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clo\u00e9","family":"Gadenne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natascha","family":"Roehlen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Saviano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Felli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pessaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Tuan","family":"Duong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Ohdan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Aikata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuaki","family":"Chayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Baumert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Schuster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jhepr.2024.101252_bib1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/S0140-6736(22)01200-4","article-title":"Hepatocellular carcinoma","volume":"400","author":"Vogel","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.jhepr.2024.101252_bib2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/s41575-020-00381-6","article-title":"Global epidemiology of NAFLD-related HCC: trends, predictions, risk factors and prevention","volume":"18","author":"Huang","year":"2021","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"10.1016\/j.jhepr.2024.101252_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.esmoop.2023.101567","article-title":"The management of hepatocellular carcinoma. Current expert opinion and recommendations derived from the 24th ESMO\/World Congress on Gastrointestinal Cancer, Barcelona, 2022","volume":"8","author":"Ducreux","year":"2023","journal-title":"ESMO Open"},{"key":"10.1016\/j.jhepr.2024.101252_bib4","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41575-022-00704-9","article-title":"Evolving therapeutic landscape of advanced hepatocellular carcinoma","volume":"20","author":"Yang","year":"2023","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"10.1016\/j.jhepr.2024.101252_bib5","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.3390\/cancers13112621","article-title":"The endless sources of hepatocellular carcinoma heterogeneity","volume":"13","author":"Barcena-Varela","year":"2021","journal-title":"Cancers"},{"key":"10.1016\/j.jhepr.2024.101252_bib6","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.2147\/JHC.S381764","article-title":"Tumor microenvironment in hepatocellular carcinoma: key players for immunotherapy","volume":"9","author":"Feng","year":"2022","journal-title":"J\u00a0Hepatocell Carcinoma"},{"key":"10.1016\/j.jhepr.2024.101252_bib7","doi-asserted-by":"crossref","DOI":"10.1063\/5.0057773","article-title":"In\u00a0vitro 3D liver tumor microenvironment models for immune cell therapy optimization","volume":"5","author":"Lam","year":"2021","journal-title":"APL Bioeng"},{"key":"10.1016\/j.jhepr.2024.101252_bib8","article-title":"Organoids to model liver disease","volume":"3","author":"Nuciforo","year":"2021","journal-title":"JHEP Rep"},{"key":"10.1016\/j.jhepr.2024.101252_bib9","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.3389\/fonc.2019.01376","article-title":"The extracellular, cellular, and nuclear stiffness, a trinity in the cancer resistome-a review","volume":"9","author":"Deville","year":"2019","journal-title":"Front Oncol"},{"key":"10.1016\/j.jhepr.2024.101252_bib10","doi-asserted-by":"crossref","first-page":"874","DOI":"10.3390\/cancers13040874","article-title":"Organoid and spheroid tumor models: techniques and applications","volume":"13","author":"Gunti","year":"2021","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.jhepr.2024.101252_bib11","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41586-019-1373-2","article-title":"A human liver cell atlas reveals heterogeneity and epithelial progenitors","volume":"572","author":"Aizarani","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.jhepr.2024.101252_bib12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s13045-019-0782-x","article-title":"Heterogeneity of cancer-associated fibroblasts and roles in the progression, prognosis, and therapy of hepatocellular carcinoma","volume":"12","author":"Yin","year":"2019","journal-title":"J Hematol Oncol"},{"key":"10.1016\/j.jhepr.2024.101252_bib13","doi-asserted-by":"crossref","first-page":"7385","DOI":"10.1158\/0008-5472.CAN-09-1089","article-title":"Integrative transcriptome analysis reveals common molecular subclasses of human hepatocellular carcinoma","volume":"69","author":"Hoshida","year":"2009","journal-title":"Cancer Res"},{"key":"10.1016\/j.jhepr.2024.101252_bib14","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s12935-022-02599-7","article-title":"Cancer-associated fibroblasts and resistance to anticancer therapies: status, mechanisms, and countermeasures","volume":"22","author":"Feng","year":"2022","journal-title":"Cancer Cell Int"},{"key":"10.1016\/j.jhepr.2024.101252_bib15","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0022-1759(00)00327-6","article-title":"Specific affinity depletion of cell adhesion molecules and growth factors from serum","volume":"247","author":"Underwood","year":"2001","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.jhepr.2024.101252_bib16","article-title":"The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma","volume":"8","author":"Kong","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jhepr.2024.101252_bib17","doi-asserted-by":"crossref","first-page":"226","DOI":"10.3390\/cancers14010226","article-title":"Tumor-associated macrophages in hepatocellular carcinoma pathogenesis, prognosis and therapy","volume":"14","author":"Arvanitakis","year":"2022","journal-title":"Cancers"},{"key":"10.1016\/j.jhepr.2024.101252_bib18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0531-5565(98)00058-8","article-title":"CD69, CD25, and HLA-DR activation antigen expression on CD3+ lymphocytes and relationship to serum TNF-\u03b1, IFN-\u03b3, and sIL-2R levels in aging","volume":"34","author":"Rea","year":"1999","journal-title":"Exp Gerontol"},{"key":"10.1016\/j.jhepr.2024.101252_bib19","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1158\/2326-6066.CIR-14-0040","article-title":"In vitro characterization of the Anti-PD-1 antibody nivolumab, BMS-936558, and in vivo toxicology in non-human primates","volume":"2","author":"Wang","year":"2014","journal-title":"Cancer Immunol Res"},{"key":"10.1016\/j.jhepr.2024.101252_bib20","doi-asserted-by":"crossref","first-page":"8370","DOI":"10.1038\/s41598-022-12136-1","article-title":"In vitro preliminary study on different anti-PD-1 antibody concentrations on T cells activation","volume":"12","author":"Wieleba","year":"2022","journal-title":"Sci Rep"},{"key":"10.1016\/j.jhepr.2024.101252_bib21","doi-asserted-by":"crossref","first-page":"5525","DOI":"10.3390\/cancers14225525","article-title":"Lenvatinib as first-line treatment for unresectable hepatocellular carcinoma: a systematic review and meta-analysis","volume":"14","author":"Wang","year":"2022","journal-title":"Cancers"},{"key":"10.1016\/j.jhepr.2024.101252_bib22","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2147\/JHC.S268305","article-title":"Comparison of efficacy of systemic therapies in advanced hepatocellular carcinoma: updated systematic review and frequentist network meta-analysis of randomized controlled trials","volume":"8","author":"Park","year":"2021","journal-title":"J Hepatocell Carcinoma"},{"key":"10.1016\/j.jhepr.2024.101252_bib23","doi-asserted-by":"crossref","first-page":"4878","DOI":"10.1038\/s41467-022-32473-z","article-title":"Single-cell analysis of hepatoblastoma identifies tumor signatures that predict chemotherapy susceptibility using patient-specific tumor spheroids","volume":"13","author":"Song","year":"2022","journal-title":"Nat Commun"},{"key":"10.1016\/j.jhepr.2024.101252_bib24","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s13046-018-0752-0","article-title":"Patient-derived multicellular tumor spheroids towards optimized treatment for patients with hepatocellular carcinoma","volume":"37","author":"Song","year":"2018","journal-title":"J Exp Clin Cancer Res"},{"key":"10.1016\/j.jhepr.2024.101252_bib25","doi-asserted-by":"crossref","first-page":"1087656","DOI":"10.3389\/fbioe.2022.1087656","article-title":"Generation of multicellular tumor spheroids with micro-well array for anticancer drug combination screening based on a valuable biomarker of hepatocellular carcinoma","volume":"10","author":"Wang","year":"2022","journal-title":"Front Bioeng Biotechnol"},{"key":"10.1016\/j.jhepr.2024.101252_bib26","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.3390\/nano11123233","article-title":"Complex tumor spheroid formation and one-step cancer-associated fibroblasts purification from hepatocellular carcinoma tissue promoted by inorganic surface topography","volume":"11","author":"Dituri","year":"2021","journal-title":"Nanomaterials"},{"key":"10.1016\/j.jhepr.2024.101252_bib27","article-title":"Three-dimensional spheroid model to investigate the tumor-stromal interaction in hepatocellular carcinoma","volume":"175","author":"Zaki","year":"2021","journal-title":"J Vis Exp"},{"key":"10.1016\/j.jhepr.2024.101252_bib28","doi-asserted-by":"crossref","first-page":"20160877","DOI":"10.1098\/rsif.2016.0877","article-title":"Advances in multicellular spheroids formation","volume":"14","author":"Cui","year":"2017","journal-title":"J R Soc Interface"},{"key":"10.1016\/j.jhepr.2024.101252_bib29","doi-asserted-by":"crossref","first-page":"104826","DOI":"10.1016\/j.ebiom.2023.104826","article-title":"Patient-derived precision cut tissue slices from primary liver cancer as a potential platform for preclinical drug testing","volume":"97","author":"Jagatia","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.jhepr.2024.101252_bib30","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.medj.2021.03.017","article-title":"A blood-based prognostic liver secretome signature and long-term hepatocellular carcinoma risk in advanced liver fibrosis","volume":"2","author":"Fujiwara","year":"2021","journal-title":"Med"},{"key":"10.1016\/j.jhepr.2024.101252_bib31","doi-asserted-by":"crossref","first-page":"eaaz0274","DOI":"10.1126\/scisignal.aaz0274","article-title":"The human secretome","volume":"12","author":"Uhl\u00e9n","year":"2019","journal-title":"Sci Signal"}],"container-title":["JHEP Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555924002568?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555924002568?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T00:58:25Z","timestamp":1740877105000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589555924002568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["S2589555924002568"],"URL":"https:\/\/doi.org\/10.1016\/j.jhepr.2024.101252","relation":{},"ISSN":["2589-5559"],"issn-type":[{"value":"2589-5559","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response","name":"articletitle","label":"Article Title"},{"value":"JHEP Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jhepr.2024.101252","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier B.V. on behalf of European Association for the Study of the Liver (EASL).","name":"copyright","label":"Copyright"}],"article-number":"101252"}}