{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:49:34Z","timestamp":1772862574640,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004784","name":"Novartis Stiftung\u00a0f\u00fcr\u00a0Medizinisch-Biologische Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["23C204"],"award-info":[{"award-number":["23C204"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["310030_207477"],"award-info":[{"award-number":["310030_207477"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jhep-reports.eu","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["JHEP Reports"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.jhepr.2024.101293","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T22:23:54Z","timestamp":1733437434000},"page":"101293","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein"],"prefix":"10.1016","volume":"7","author":[{"given":"Maliki","family":"Ankavay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Poll\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"No\u00e9mie","family":"Oechslin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0858-6124","authenticated-orcid":false,"given":"Katja","family":"Dinkelborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Behrendt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1977-6792","authenticated-orcid":false,"given":"Darius","family":"Moradpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-1494","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Gouttenoire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jhepr.2024.101293_bib1","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2017.86","article-title":"Hepatitis E virus infection","volume":"3","author":"Kamar","year":"2017","journal-title":"Nat Rev Dis Primers"},{"issue":"6","key":"10.1016\/j.jhepr.2024.101293_bib2","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.jhep.2018.03.005","article-title":"EASL Clinical Practice Guidelines on hepatitis E virus infection","volume":"68","year":"2018","journal-title":"J\u00a0Hepatol"},{"issue":"4","key":"10.1016\/j.jhepr.2024.101293_bib3","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1002\/hep.25505","article-title":"The global burden of hepatitis E virus genotypes 1 and 2 in 2005","volume":"55","author":"Rein","year":"2012","journal-title":"Hepatology"},{"issue":"4","key":"10.1016\/j.jhepr.2024.101293_bib4","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/hep.28436","article-title":"A\u00a0nationwide survey of hepatitis E viral infection in French blood donors","volume":"63","author":"Mansuy","year":"2016","journal-title":"Hepatology"},{"issue":"5","key":"10.1016\/j.jhepr.2024.101293_bib5","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1093\/cid\/ciz953","article-title":"Ribavirin for hepatitis E virus infection after organ transplantation: a large european retrospective multicenter study","volume":"71","author":"Kamar","year":"2020","journal-title":"Clin Infect Dis"},{"issue":"9","key":"10.1016\/j.jhepr.2024.101293_bib6","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.001778","article-title":"ICTV Virus Taxonomy Profile: Hepeviridae 2022","volume":"103","author":"Purdy","year":"2022","journal-title":"J\u00a0Gen Virol"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib7","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jhep.2016.02.045","article-title":"Update on hepatitis E virology: implications for clinical practice","volume":"65","author":"Debing","year":"2016","journal-title":"J\u00a0Hepatol"},{"issue":"2","key":"10.1016\/j.jhepr.2024.101293_bib8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nrgastro.2017.150","article-title":"Hepatitis E virus: advances and challenges","volume":"15","author":"Nimgaonkar","year":"2018","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib9","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1053\/j.gastro.2017.09.020","article-title":"Hepatitis E virus lifecycle and identification of 3 forms of the ORF2 capsid protein","volume":"154","author":"Montpellier","year":"2018","journal-title":"Gastroenterology"},{"issue":"18","key":"10.1016\/j.jhepr.2024.101293_bib10","doi-asserted-by":"crossref","first-page":"4773","DOI":"10.1073\/pnas.1721345115","article-title":"Origin, antigenicity, and function of a secreted form of ORF2 in hepatitis E virus infection","volume":"115","author":"Yin","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib11","doi-asserted-by":"crossref","first-page":"6243","DOI":"10.1038\/s41598-019-42737-2","article-title":"New insights into the ORF2 capsid protein, a key player of the hepatitis E virus lifecycle","volume":"9","author":"Ankavay","year":"2019","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib12","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev-virology-031413-085359","article-title":"Naked viruses that aren't always naked: quasi-enveloped agents of acute hepatitis","volume":"1","author":"Feng","year":"2014","journal-title":"Annu Rev Virol"},{"issue":"5","key":"10.1016\/j.jhepr.2024.101293_bib13","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.3390\/cells9051294","article-title":"On the host side of the hepatitis E virus life cycle","volume":"9","author":"Oechslin","year":"2020","journal-title":"Cells"},{"issue":"4","key":"10.1016\/j.jhepr.2024.101293_bib14","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.tim.2020.07.002","article-title":"Virus-host cell interplay\u00a0during hepatitis E virus infection","volume":"29","author":"Wissing","year":"2021","journal-title":"Trends Microbiol"},{"issue":"4","key":"10.1016\/j.jhepr.2024.101293_bib15","doi-asserted-by":"crossref","first-page":"869","DOI":"10.3390\/v15040869","article-title":"Expanding the hepatitis E virus toolbox: selectable replicons and recombinant reporter genomes","volume":"15","author":"Oechslin","year":"2023","journal-title":"Viruses"},{"issue":"6","key":"10.1016\/j.jhepr.2024.101293_bib16","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01906-21","article-title":"Development of recombinant infectious hepatitis E virus harboring the nanoKAZ gene and its application in drug screening","volume":"96","author":"Primadharsini","year":"2022","journal-title":"J\u00a0Virol"},{"issue":"19","key":"10.1016\/j.jhepr.2024.101293_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00459-19","article-title":"Recombinant hepatitis E viruses harboring tags in the ORF1 protein","volume":"93","author":"Szkolnicka","year":"2019","journal-title":"J\u00a0Virol"},{"key":"10.1016\/j.jhepr.2024.101293_bib18","author":"Kelly","year":"1994"},{"issue":"21","key":"10.1016\/j.jhepr.2024.101293_bib19","doi-asserted-by":"crossref","first-page":"10457","DOI":"10.1128\/JVI.00892-06","article-title":"ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in\u00a0vitro","volume":"80","author":"Emerson","year":"2006","journal-title":"J\u00a0Virol"},{"issue":"24","key":"10.1016\/j.jhepr.2024.101293_bib20","doi-asserted-by":"crossref","first-page":"13001","DOI":"10.1128\/JVI.76.24.13001-13014.2002","article-title":"Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication","volume":"76","author":"Blight","year":"2002","journal-title":"J\u00a0Virol"},{"issue":"12","key":"10.1016\/j.jhepr.2024.101293_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007471","article-title":"Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion","volume":"14","author":"Gouttenoire","year":"2018","journal-title":"PLoS Pathog"},{"issue":"10","key":"10.1016\/j.jhepr.2024.101293_bib22","doi-asserted-by":"crossref","first-page":"6031","DOI":"10.1128\/JVI.00444-13","article-title":"The hepatitis E virus capsid C-terminal\u00a0region is essential for the viral life cycle: implication for viral genome encapsidation and particle stabilization","volume":"87","author":"Shiota","year":"2013","journal-title":"J\u00a0Virol"},{"issue":"10","key":"10.1016\/j.jhepr.2024.101293_bib23","doi-asserted-by":"crossref","first-page":"5697","DOI":"10.1128\/JVI.00146-12","article-title":"Adaptation of a genotype 3 hepatitis E\u00a0virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination","volume":"86","author":"Shukla","year":"2012","journal-title":"J\u00a0Virol"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib24","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1002\/hep.32100","article-title":"Hepatitis E virus RNA-dependent RNA polymerase is involved in RNA replication and infectious particle production","volume":"75","author":"Oechslin","year":"2022","journal-title":"Hepatology"},{"issue":"3","key":"10.1016\/j.jhepr.2024.101293_bib25","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.jhep.2017.04.002","article-title":"Visualization of hepatitis E virus RNA and proteins in the human liver","volume":"67","author":"Lenggenhager","year":"2017","journal-title":"J\u00a0Hepatol"},{"issue":"8","key":"10.1016\/j.jhepr.2024.101293_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010798","article-title":"An arginine-rich motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell","volume":"18","author":"Hervouet","year":"2022","journal-title":"PLoS Pathog"},{"issue":"2","key":"10.1016\/j.jhepr.2024.101293_bib27","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1021\/acschembio.7b00549","article-title":"CRISPR-mediated tagging of endogenous proteins with a luminescent peptide","volume":"13","author":"Schwinn","year":"2018","journal-title":"ACS Chem Biol"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib28","doi-asserted-by":"crossref","first-page":"6895","DOI":"10.1038\/s41598-019-43319-y","article-title":"HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions","volume":"9","author":"Ranawakage","year":"2019","journal-title":"Sci Rep"},{"issue":"4","key":"10.1016\/j.jhepr.2024.101293_bib29","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.1128\/AAC.02427-15","article-title":"Antiviral activities of different Interferon types and subtypes against hepatitis E virus replication","volume":"60","author":"Todt","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib30","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1053\/j.gastro.2015.09.011","article-title":"Sofosbuvir inhibits hepatitis E virus replication in\u00a0vitro and results in an additive effect when combined with ribavirin","volume":"150","author":"Dao","year":"2016","journal-title":"Gastroenterology"},{"issue":"5","key":"10.1016\/j.jhepr.2024.101293_bib31","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1053\/j.gastro.2010.08.002","article-title":"Ribavirin therapy inhibits viral replication on patients with chronic hepatitis E virus infection","volume":"139","author":"Kamar","year":"2010","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jhepr.2024.101293_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms11046","article-title":"Versatile protein tagging in cells with split fluorescent protein","volume":"7","author":"Kamiyama","year":"2016","journal-title":"Nat Commun"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.virusres.2011.03.015","article-title":"Structure of hepatitis E viral particle","volume":"161","author":"Mori","year":"2011","journal-title":"Virus Res"},{"issue":"1","key":"10.1016\/j.jhepr.2024.101293_bib34","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens11010024","article-title":"The capsid (ORF2) protein\u00a0of\u00a0hepatitis E virus in feces is C-terminally truncated","volume":"11","author":"Nishiyama","year":"2021","journal-title":"Pathogens"},{"issue":"12","key":"10.1016\/j.jhepr.2024.101293_bib35","first-page":"987","article-title":"Rapid quantitative screening assay for SARS-CoV-2 neutralizing antibodies using HiBiT-tagged virus-like particles","volume":"12","author":"Miyakawa","year":"2020","journal-title":"J\u00a0Mol Cell Biol"},{"issue":"9","key":"10.1016\/j.jhepr.2024.101293_bib36","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1002\/hep4.2018","article-title":"A single hepatitis B virus genome with a reporter allows the entire viral life cycle to be monitored in primary human hepatocytes","volume":"6","author":"Sumiyadorj","year":"2022","journal-title":"Hepatol Commun"},{"issue":"9","key":"10.1016\/j.jhepr.2024.101293_bib37","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00508-23","article-title":"Development of a HiBiT-tagged reporter hepatitis E virus and its utility as an antiviral drug screening platform","volume":"97","author":"Nagashima","year":"2023","journal-title":"J\u00a0Virol"}],"container-title":["JHEP Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555924002970?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555924002970?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:28:56Z","timestamp":1741652936000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589555924002970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["S2589555924002970"],"URL":"https:\/\/doi.org\/10.1016\/j.jhepr.2024.101293","relation":{},"ISSN":["2589-5559"],"issn-type":[{"value":"2589-5559","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein","name":"articletitle","label":"Article Title"},{"value":"JHEP Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jhepr.2024.101293","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V. on behalf of European Association for the Study of the Liver (EASL).","name":"copyright","label":"Copyright"}],"article-number":"101293"}}