{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:35:56Z","timestamp":1781642156995,"version":"3.54.5"},"reference-count":36,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["SAF2019"],"award-info":[{"award-number":["SAF2019"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PID2019-105148RB-I00"],"award-info":[{"award-number":["PID2019-105148RB-I00"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AEI_FPI20"],"award-info":[{"award-number":["AEI_FPI20"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["FIS_FI21"],"award-info":[{"award-number":["FIS_FI21"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["FI21\/00064"],"award-info":[{"award-number":["FI21\/00064"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Carlos III Health Institute","doi-asserted-by":"publisher","award":["CM23\/00068"],"award-info":[{"award-number":["CM23\/00068"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Carlos III Health Institute","doi-asserted-by":"publisher","award":["CPII22\/00006"],"award-info":[{"award-number":["CPII22\/00006"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jhep-reports.eu","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["JHEP Reports"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.jhepr.2025.101543","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T17:06:36Z","timestamp":1754413596000},"page":"101543","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and immortalization of human portal vein endothelial cells: A novel research tool for studying splanchnic vasculature"],"prefix":"10.1016","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5228-8304","authenticated-orcid":false,"given":"Aina","family":"Anton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Shalaby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5423-8785","authenticated-orcid":false,"given":"J\u00falia","family":"Campalans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4946-1495","authenticated-orcid":false,"given":"Annabel","family":"Blasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4950-6988","authenticated-orcid":false,"given":"Yiliam","family":"Fundora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-0869","authenticated-orcid":false,"given":"Rosa","family":"Monta\u00f1\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor","family":"Garc\u00eda-Calder\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1768-9460","authenticated-orcid":false,"given":"Joana","family":"Codina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1696-7753","authenticated-orcid":false,"given":"Josepmaria","family":"Argem\u00ed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Tura-Ceide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9032-4954","authenticated-orcid":false,"given":"Joan Carles","family":"Garc\u00eda-Pag\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3155-1468","authenticated-orcid":false,"given":"Gen\u00eds","family":"Campreci\u00f3s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7937-984X","authenticated-orcid":false,"given":"Virginia","family":"Hern\u00e1ndez-Gea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jhepr.2025.101543_bib1","doi-asserted-by":"crossref","first-page":"S121","DOI":"10.1002\/hep.20993","article-title":"The hyperdynamic circulation of chronic liver diseases: from the patient to the molecule","volume":"43","author":"Iwakiri","year":"2006","journal-title":"Hepatology"},{"key":"10.1016\/j.jhepr.2025.101543_bib2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s13244-019-0716-8","article-title":"All about portal vein: a pictorial display to anatomy, variants and physiopathology","volume":"10","author":"Carneiro","year":"2019","journal-title":"Insights Imaging"},{"key":"10.1016\/j.jhepr.2025.101543_bib3","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.jaci.2023.10.029","article-title":"High metabolite concentrations in portal venous blood as a possible mechanism for microbiota effects on the immune system and Western diseases","volume":"153","author":"Wang","year":"2024","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jhepr.2025.101543_bib4","doi-asserted-by":"crossref","first-page":"E90","DOI":"10.1161\/ATVBAHA.118.310367","article-title":"Thrombotic regulation from the endothelial cell perspectives","volume":"38","author":"Wang","year":"2018","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1016\/j.jhepr.2025.101543_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12872-015-0124-z","article-title":"Endothelial cell control of thrombosis","volume":"15","author":"Yau","year":"2015","journal-title":"BMC Cardiovasc Disord"},{"key":"10.1016\/j.jhepr.2025.101543_bib6","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1055\/s-0040-1713169","article-title":"Endothelial damage of the portal vein is associated with Heparin-like effect in advanced stages of cirrhosis","volume":"120","author":"Shalaby","year":"2020","journal-title":"Thromb Haemost"},{"key":"10.1016\/j.jhepr.2025.101543_bib7","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/j.jhep.2017.07.002","article-title":"Gut-derived endotoxin stimulates factor VIII secretion from endothelial cells. Implications for hypercoagulability in cirrhosis","volume":"67","author":"Carnevale","year":"2017","journal-title":"J Hepatol"},{"key":"10.1016\/j.jhepr.2025.101543_bib8","doi-asserted-by":"crossref","first-page":"800","DOI":"10.3390\/jcm11030800","article-title":"The pathophysiology of portal vein thrombosis in cirrhosis: getting deeper into Virchow\u2019s triad","volume":"11","author":"Anton","year":"2022","journal-title":"J Clin Med"},{"key":"10.1016\/j.jhepr.2025.101543_bib9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3109\/00313028909059547","article-title":"A comparison of structural features of the walls of coronary arteries from 10 different species","volume":"21","author":"Sims","year":"1989","journal-title":"Pathology"},{"key":"10.1016\/j.jhepr.2025.101543_bib10","article-title":"Experimental rat and mouse carotid artery surgery: injury & remodeling studies","volume":"2013","author":"Holt","year":"2013","journal-title":"ISRN Minim Invasive Surg"},{"key":"10.1016\/j.jhepr.2025.101543_bib11","article-title":"A preclinical porcine model of portal vein thrombosis in liver cirrhosis","volume":"2020","author":"Zhang","year":"2020","journal-title":"Biomed Res Int"},{"key":"10.1016\/j.jhepr.2025.101543_bib12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/abbs\/gmaa165","article-title":"A new modified canine model of portal vein thrombosis","volume":"53","author":"Deng","year":"2021","journal-title":"Acta Biochim Biophys Sin (Shanghai)"},{"key":"10.1016\/j.jhepr.2025.101543_bib13","doi-asserted-by":"crossref","first-page":"H958","DOI":"10.1152\/ajpheart.00220.2022","article-title":"Loss of lung microvascular endothelial Piezo2 expression impairs NO synthesis, induces EndMT, and is associated with pulmonary hypertension","volume":"323","author":"Tian","year":"2022","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"10.1016\/j.jhepr.2025.101543_bib14","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1182\/blood-2009-10-250910","article-title":"Endothelial-derived tissue factor pathway inhibitor regulates arterial thrombosis but is not required for development or hemostasis","volume":"116","author":"White","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.jhepr.2025.101543_bib15","doi-asserted-by":"crossref","first-page":"6600","DOI":"10.1074\/jbc.M116.742627","article-title":"Nitric-oxide synthase trafficking inducer is a pleiotropic regulator of endothelial cell function and signaling","volume":"292","author":"Chakraborty","year":"2017","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jhepr.2025.101543_bib16","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.3892\/mmr.2017.6240","article-title":"Slit2 suppresses endothelial cell proliferation and migration by inhibiting the VEGF-Notch signaling pathway","volume":"15","author":"Li","year":"2017","journal-title":"Mol Med Rep"},{"key":"10.1016\/j.jhepr.2025.101543_bib17","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1186\/s12887-024-04766-3","article-title":"Semaphorin 7A promotes endothelial permeability and inflammation via plexin C1 and integrin \u03b21 in Kawasaki disease","volume":"24","author":"Huang","year":"2024","journal-title":"BMC Pediatr"},{"key":"10.1016\/j.jhepr.2025.101543_bib18","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0165-2478(03)00018-X","article-title":"IL-7 receptor is present on human microvascular endothelial cells","volume":"86","author":"Du\u015b","year":"2003","journal-title":"Immunol Lett"},{"key":"10.1016\/j.jhepr.2025.101543_bib19","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.14954","article-title":"m6A reader IGF2BP1 accelerates apoptosis of high glucose-induced vascular endothelial cells in a m6A\u2013HMGB1 dependent manner","volume":"11","author":"Liang","year":"2023","journal-title":"PeerJ"},{"key":"10.1016\/j.jhepr.2025.101543_bib20","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1161\/CIRCULATIONAHA.120.049497","article-title":"Cyclin D2 overexpression enhances the efficacy of human induced pluripotent stem cell-derived cardiomyocytes for myocardial repair in a swine model of myocardial infarction","volume":"144","author":"Zhao","year":"2021","journal-title":"Circulation"},{"key":"10.1016\/j.jhepr.2025.101543_bib21","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1161\/CIRCRESAHA.116.308870","article-title":"TWIST1 integrates endothelial responses to flow in vascular dysfunction and atherosclerosis","volume":"119","author":"Mahmoud","year":"2016","journal-title":"Circ Res"},{"key":"10.1016\/j.jhepr.2025.101543_bib22","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.3390\/ijms23073769","article-title":"Context dependent Sulf1\/Sulf2 functional divergence in endothelial cell activity","volume":"23","author":"Justo","year":"2022","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jhepr.2025.101543_bib23","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1083\/jcb.99.6.2034","article-title":"Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein","volume":"99","author":"Voyta","year":"1984","journal-title":"J Cell Biol"},{"key":"10.1016\/j.jhepr.2025.101543_bib24","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0021-9150(85)90086-3","article-title":"High-affinity uptake and degradation of acetylated low density lipoprotein by confluent human vascular endothelial cells","volume":"56","author":"Havekes","year":"1985","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.jhepr.2025.101543_bib25","doi-asserted-by":"crossref","first-page":"208","DOI":"10.4254\/wjh.v2.i6.208","article-title":"Physiopathology of splanchnic vasodilation in portal hypertension","volume":"2","author":"Martell","year":"2010","journal-title":"World J Hepatol"},{"key":"10.1016\/j.jhepr.2025.101543_bib26","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1478-3231.2011.02579.x","article-title":"Endothelial dysfunction in the regulation of portal hypertension","volume":"32","author":"Iwakiri","year":"2012","journal-title":"Liver Int"},{"key":"10.1016\/j.jhepr.2025.101543_bib27","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jhep.2012.03.007","article-title":"Functional aspects on the pathophysiology of portal hypertension in cirrhosis","volume":"57","author":"Garc\u00eda-Pag\u00e1n","year":"2012","journal-title":"J Hepatol"},{"issue":"Suppl 1","key":"10.1016\/j.jhepr.2025.101543_bib28","doi-asserted-by":"crossref","first-page":"S68","DOI":"10.1016\/j.jhep.2008.01.021","article-title":"The management of portal hypertension: rational basis, available treatments and future options","volume":"48","author":"Bosch","year":"2008","journal-title":"J Hepatol"},{"key":"10.1016\/j.jhepr.2025.101543_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0218716","article-title":"Functionalized cerium oxide nanoparticles mitigate the oxidative stress and pro-inflammatory activity associated to the portal vein endothelium of cirrhotic rats","volume":"14","author":"Ribera","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.jhepr.2025.101543_bib30","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/S0955-2863(99)00032-7","article-title":"The influence of zinc status on the kinetics of zinc uptake into cultured endothelial cells","volume":"10","author":"McClung","year":"1999","journal-title":"J Nutr Biochem"},{"key":"10.1016\/j.jhepr.2025.101543_bib31","doi-asserted-by":"crossref","first-page":"E773","DOI":"10.1152\/ajpendo.00507.2003","article-title":"Glucose-dependent insulinotropic peptide: differential effects on hepatic artery vs. portal vein endothelial cells","volume":"286","author":"Ding","year":"2004","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"10.1016\/j.jhepr.2025.101543_bib32","article-title":"Portal vein variations, clinical correlation, and embryological explanation: a review article","volume":"15","author":"Tyagi","year":"2023","journal-title":"Cureus"},{"key":"10.1016\/j.jhepr.2025.101543_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0047-6374(97)00067-5","article-title":"Cell aging in vivo and in vitro","volume":"98","author":"Rubin","year":"1997","journal-title":"Mech Ageing Dev"},{"key":"10.1016\/j.jhepr.2025.101543_bib34","doi-asserted-by":"crossref","first-page":"5658","DOI":"10.1016\/j.biomaterials.2005.02.024","article-title":"Effect of passage number and matrix characteristics on differentiation of endothelial cells cultured for tissue engineering","volume":"26","author":"Chennazhy","year":"2005","journal-title":"Biomaterials"},{"key":"10.1016\/j.jhepr.2025.101543_bib35","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1038\/s41598-020-59213-x","article-title":"Gene expression in immortalized versus primary isolated cardiac endothelial cells","volume":"10","author":"Deng","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.jhepr.2025.101543_bib36","first-page":"1545","article-title":"Gene expression analysis in SV40-immortalized human breast luminal epithelial cells with stem cell characteristics using a cDNA microarray","volume":"24","author":"Park","year":"2004","journal-title":"Int J Oncol"}],"container-title":["JHEP Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555925002228?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555925002228?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T20:23:05Z","timestamp":1772914985000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589555925002228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["S2589555925002228"],"URL":"https:\/\/doi.org\/10.1016\/j.jhepr.2025.101543","relation":{},"ISSN":["2589-5559"],"issn-type":[{"value":"2589-5559","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation and immortalization of human portal vein endothelial cells: A novel research tool for studying splanchnic vasculature","name":"articletitle","label":"Article Title"},{"value":"JHEP Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jhepr.2025.101543","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V. on behalf of European Association for the Study of the Liver (EASL).","name":"copyright","label":"Copyright"}],"article-number":"101543"}}