{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:15:30Z","timestamp":1783584930518,"version":"3.55.0"},"reference-count":25,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["823 _00"],"award-info":[{"award-number":["823 _00"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TTU 05"],"award-info":[{"award-number":["TTU 05"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["BR 7111 \/1-1"],"award-info":[{"award-number":["BR 7111 \/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["STE 1954 \/16-1"],"award-info":[{"award-number":["STE 1954 \/16-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005629","name":"Stiftung Tier\u00e4rztliche Hochschule Hannover","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005629","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024886","name":"Friedrich-Loeffler-Institut","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024886","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006254","name":"Ruhr-Universit\u00e4t Bochum","doi-asserted-by":"publisher","award":["F1030-2021"],"award-info":[{"award-number":["F1030-2021"]}],"id":[{"id":"10.13039\/501100006254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jhep-reports.eu","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["JHEP Reports"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.jhepr.2026.101885","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:29:25Z","timestamp":1777876165000},"page":"101885","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a CRISPR-Cas13-based antiviral strategy against hepatitis E virus"],"prefix":"10.1016","volume":"8","author":[{"given":"Emely","family":"Richter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Kl\u00f6hn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian K.","family":"Nocke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3001-4748","authenticated-orcid":false,"given":"Marcel Edgar","family":"Friedrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Todt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eike","family":"Steinmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8790-0022","authenticated-orcid":false,"given":"Yannick","family":"Br\u00fcggemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jhepr.2026.101885_bib1","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1002\/hep.25505","article-title":"The global burden of hepatitis E virus genotypes 1 and 2 in 2005","volume":"55","author":"Rein","year":"2012","journal-title":"Hepatol (Baltimore, Md.)"},{"key":"10.1016\/j.jhepr.2026.101885_bib2","series-title":"Hepatitis E","year":"2025"},{"key":"10.1016\/j.jhepr.2026.101885_bib3","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2002324118","article-title":"Ranking the risk of animal-to-human spillover for newly discovered viruses","volume":"118","author":"Grange","year":"2021","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jhepr.2026.101885_bib4","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1053\/j.gastro.2012.02.014","article-title":"Pathogenesis and treatment of hepatitis e virus infection","volume":"142","author":"Wedemeyer","year":"2012","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jhepr.2026.101885_bib5","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.jhep.2010.08.037","article-title":"High viral load and deregulation of the progesterone receptor signaling pathway: association with hepatitis E-related poor pregnancy outcome","volume":"54","author":"Bose","year":"2011","journal-title":"J Hepatol"},{"key":"10.1016\/j.jhepr.2026.101885_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2024.106062","article-title":"Host-targeting antivirals for chronic viral infections of the liver","volume":"234","author":"Frericks","year":"2025","journal-title":"Antivir Res"},{"key":"10.1016\/j.jhepr.2026.101885_bib7","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.jhep.2018.03.005","article-title":"EASL clinical practice guidelines on hepatitis E virus infection","volume":"68","year":"2018","journal-title":"J Hepatol"},{"key":"10.1016\/j.jhepr.2026.101885_bib8","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1056\/NEJMoa1215246","article-title":"Ribavirin for chronic hepatitis E virus infection in transplant recipients","volume":"370","author":"Kamar","year":"2014","journal-title":"The New Engl J Med"},{"key":"10.1016\/j.jhepr.2026.101885_bib9","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1053\/j.gastro.2014.08.040","article-title":"A mutation in the hepatitis E virus RNA polymerase promotes its replication and associates with ribavirin treatment failure in organ transplant recipients","volume":"147","author":"Debing","year":"2014","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jhepr.2026.101885_bib10","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jhep.2016.02.045","article-title":"Update on hepatitis E virology: implications for clinical practice","volume":"65","author":"Debing","year":"2016","journal-title":"J Hepatol"},{"key":"10.1016\/j.jhepr.2026.101885_bib11","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1097\/HEP.0000000000000514","article-title":"Emergence of resistance-associated variants during sofosbuvir treatment in chronically infected hepatitis E patients","volume":"78","author":"G\u00f6mer","year":"2023","journal-title":"Hepatology"},{"key":"10.1016\/j.jhepr.2026.101885_bib12","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1136\/gutjnl-2015-311000","article-title":"In vivo evidence for ribavirin-induced mutagenesis of the hepatitis E virus genome","volume":"65","author":"Todt","year":"2016","journal-title":"Gut"},{"key":"10.1016\/j.jhepr.2026.101885_bib13","article-title":"RNA-targeting CRISPR-Cas systems","author":"van Beljouw","year":"2022","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jhepr.2026.101885_bib14","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.cell.2018.02.033","article-title":"Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors","volume":"173","author":"Konermann","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.jhepr.2026.101885_bib15","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/s41587-021-00822-w","article-title":"Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents","volume":"39","author":"Blanchard","year":"2021","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jhepr.2026.101885_bib16","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.cell.2020.04.020","article-title":"Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza","volume":"181","author":"Abbott","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.jhepr.2026.101885_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2023.104682","article-title":"AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected mice","volume":"93","author":"Keng","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.jhepr.2026.101885_bib18","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/s41587-020-0456-9","article-title":"Massively parallel Cas13 screens reveal principles for guide RNA design","volume":"38","author":"Wessels","year":"2020","journal-title":"Nat Biotechnol"},{"issue":"42","key":"10.1016\/j.jhepr.2026.101885_bib19","article-title":"Engineered, nucleocytoplasmic shuttling Cas13d enables highly efficient cytosolic RNA targeting","volume":"10","author":"Gruber","year":"2024","journal-title":"Cell Discov"},{"key":"10.1016\/j.jhepr.2026.101885_bib20","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/s41589-024-01593-6","article-title":"Structures, mechanisms and applications of RNA-centric CRISPR-Cas13","volume":"20","author":"Yang","year":"2024","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.jhepr.2026.101885_bib21","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1038\/s41467-022-30546-7","article-title":"Broad-spectrum CRISPR-mediated inhibition of SARS-CoV-2 variants and endemic coronaviruses in vitro","volume":"13","author":"Zeng","year":"2022","journal-title":"Nat Commun"},{"key":"10.1016\/j.jhepr.2026.101885_bib22","article-title":"A comprehensive analysis and resource to use CRISPR-Cas13 for broad-spectrum targeting of RNA viruses","volume":"2","author":"Lin","year":"2021","journal-title":"Cell Rep Med"},{"key":"10.1016\/j.jhepr.2026.101885_bib23","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202217146","article-title":"Optimizing the Cas13 antiviral train: cargo and delivery","volume":"15","author":"Sharma","year":"2023","journal-title":"EMBO Mol Med"},{"key":"10.1016\/j.jhepr.2026.101885_bib24","article-title":"Precise RNA targeting with CRISPR-Cas13d","author":"Hart","year":"2025","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jhepr.2026.101885_bib25","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/s41587-022-01649-9","article-title":"Precise transcript targeting by CRISPR-Csm complexes","volume":"41","author":"Colognori","year":"2023","journal-title":"Nat Biotechnol"}],"container-title":["JHEP Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555926001564?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589555926001564?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T07:30:14Z","timestamp":1783582214000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589555926001564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["S2589555926001564"],"URL":"https:\/\/doi.org\/10.1016\/j.jhepr.2026.101885","relation":{},"ISSN":["2589-5559"],"issn-type":[{"value":"2589-5559","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a CRISPR-Cas13-based antiviral strategy against hepatitis E virus","name":"articletitle","label":"Article Title"},{"value":"JHEP Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jhepr.2026.101885","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V. on behalf of European Association for the Study of the Liver (EASL).","name":"copyright","label":"Copyright"}],"article-number":"101885"}}