{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T23:27:02Z","timestamp":1772926022568,"version":"3.50.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100007903","name":"College of Public Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008529","name":"Chiba University","doi-asserted-by":"publisher","award":["17-29"],"award-info":[{"award-number":["17-29"]}],"id":[{"id":"10.13039\/501100008529","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010793","name":"Pfizer Japan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008529","name":"Chiba University","doi-asserted-by":"publisher","award":["2017\u201312"],"award-info":[{"award-number":["2017\u201312"]}],"id":[{"id":"10.13039\/501100008529","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jiac-j.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection and Chemotherapy"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.jiac.2019.02.007","type":"journal-article","created":{"date-parts":[[2019,6,22]],"date-time":"2019-06-22T02:02:47Z","timestamp":1561168967000},"page":"750-757","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular typing, antibiotic susceptibility, and biofilm production in nonencapsulated Streptococcus pneumoniae isolated from children in Japan"],"prefix":"10.1016","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3659-865X","authenticated-orcid":false,"given":"Noriko","family":"Takeuchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misako","family":"Ohkusu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Wada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Kurosawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Miyabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moon H.","family":"Nahm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naruhiko","family":"Ishiwada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jiac.2019.02.007_bib1","doi-asserted-by":"crossref","first-page":"6054","DOI":"10.1016\/j.vaccine.2015.07.069","article-title":"Nationwide population-based surveillance of invasive pneumococcal disease in Japanese children: effects of the seven-valent pneumococcal conjugate vaccine","volume":"33","author":"Suga","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.jiac.2019.02.007_bib2","doi-asserted-by":"crossref","first-page":"4813","DOI":"10.1016\/j.vaccine.2015.07.080","article-title":"Impact of the 13-valent pneumococcal conjugate vaccine (pcv13) on invasive pneumococcal disease and carriage in Alaska","volume":"33","author":"Bruce","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.jiac.2019.02.007_bib3","doi-asserted-by":"crossref","first-page":"e197","DOI":"10.1016\/j.ijid.2009.05.010","article-title":"Burden of invasive pneumococcal disease and serotype distribution among Streptococcus pneumoniae isolates in young children in Europe: impact of the 7-valentpneumococcalconjugatevaccine and considerations for future conjugate vaccines","volume":"14","author":"Isaacman","year":"2010","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.jiac.2019.02.007_bib4","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1016\/j.vaccine.2016.02.045","article-title":"The impact of private use of PCV7 in 2009 and 2010 on serotypes and antimicrobial resistance of Streptococcus pneumoniae carried by young children in Portugal: comparison with data obtained since 1996 generating a 15-year study prior to PCV13 introduction","volume":"34","author":"Nunes","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.jiac.2019.02.007_bib5","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1099\/mic.0.054270-0","article-title":"Point mutations in wchA are responsible for the non-typability of two invasive Streptococcus pneumoniae isolates","volume":"158","author":"Melchiorre","year":"2012","journal-title":"Microbiology"},{"key":"10.1016\/j.jiac.2019.02.007_bib6","article-title":"Population-based analysis of invasive nontypeable pneumococci reveals that most have defective capsule synthesis genes","volume":"9","author":"Park","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jiac.2019.02.007_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00035-12","article-title":"Nontypeable pneumococci can be divided into multiple cps types, including one type expressing the novel gene pspK","volume":"3","author":"Park","year":"2012","journal-title":"mBio"},{"key":"10.1016\/j.jiac.2019.02.007_bib8","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1099\/mic.0.056580-0","article-title":"Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates","volume":"158","author":"Salter","year":"2012","journal-title":"Microbiology"},{"key":"10.1016\/j.jiac.2019.02.007_bib9","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1099\/mic.0.000073","article-title":"PspK gene prevalence and characterization of non-typable Streptococcus pneumoniae isolates from Asian countries","volume":"161","author":"Na","year":"2015","journal-title":"Microbiology"},{"key":"10.1016\/j.jiac.2019.02.007_bib10","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofu037","article-title":"Nonencapsulated Streptococcus pneumoniae cause acute otitis media in the chinchilla that is enhanced by pneumococcal surface protein K","author":"Keller","year":"2014","journal-title":"Open Forum Infect Dis"},{"key":"10.1016\/j.jiac.2019.02.007_bib11","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jiac.2015.10.006","article-title":"Molecular epidemiology of nonencapsulated Streptococcus pneumoniae among Japanese children with acute otitis media","volume":"22","author":"Hotomi","year":"2016","journal-title":"J\u00a0Infect Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib12","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1128\/JCM.43.4.1694-1698.2005","article-title":"Analysis of the genetic structure of nontypeable pneumococcal strains isolated from conjunctiva","volume":"43","author":"Berr\u00f3n","year":"2005","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1128\/jcm.16.1.8-14.1982","article-title":"Characterization of nontypable Streptococcus pneumoniae-like organisms isolated from outbreaks of conjunctivitis","volume":"16","author":"Shayegani","year":"1982","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib14","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1136\/jcp.39.8.871","article-title":"Identifying non-capsulate strains of Streptococcus pneumoniae isolated from eyes","volume":"39","author":"Pease","year":"1986","journal-title":"J\u00a0Clin Pathol"},{"key":"10.1016\/j.jiac.2019.02.007_bib15","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1128\/JCM.02243-09","article-title":"Revisiting pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes","volume":"48","author":"da Gloria Carvalho","year":"2010","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib16","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1099\/jmm.0.47793-0","article-title":"A\u00a0novel method for rapid detection of Streptococcus pneumoniae antigen in sputum and its application in adult respiratory tract infections","volume":"57","author":"Ehara","year":"2008","journal-title":"J\u00a0Med Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib17","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jiac.2015.12.004","article-title":"A\u00a0novel method for rapid detection of Streptococcus pneumoniae antigens in blood","volume":"22","author":"Fukushima","year":"2016","journal-title":"J\u00a0Infect Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib18","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1099\/00221287-144-11-3049","article-title":"A\u00a0multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease","volume":"144","author":"Enright","year":"1998","journal-title":"Microbiology"},{"key":"10.1016\/j.jiac.2019.02.007_bib19","first-page":"343","article-title":"Japanese Society of Chemotherapy","volume":"57","year":"2009","journal-title":"Jpn J Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib20","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.jiac.2016.12.012","article-title":"Emergence of quinolone-resistant strains in Streptococcus pneumoniae isolated from paediatric patients since the approval of oral fluoroquinolones in Japan","volume":"23","author":"Takeuchi","year":"2017","journal-title":"J\u00a0Infect Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib21","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1128\/CVI.00456-15","article-title":"Immunization with pneumococcal surface protein K of nonencapsulated Streptococcus pneumoniae provides protection in a mouse model of colonization","volume":"22","author":"Keller","year":"2015","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.jiac.2019.02.007_bib22","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/jmicro\/dfq082","article-title":"Scanning and negative-staining electron microscopy of protoplast regeneration of a wild-type and two chitin synthase mutants in the pathogenic yeast Candida glabrata","volume":"60","author":"Namiki","year":"2011","journal-title":"J\u00a0Electron Microsc"},{"key":"10.1016\/j.jiac.2019.02.007_bib23","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1128\/JCM.01763-10","article-title":"Analysis of invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines reveals heterogeneous behavior of clones expressing the same serotype","volume":"49","author":"S\u00e1-Le\u00e3o","year":"2011","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib24","first-page":"56","article-title":"Nonencapsulated Streptococcus pneumoniae as a cause of chronic adenoiditis","volume":"4","author":"Dixit","year":"2016"},{"key":"10.1016\/j.jiac.2019.02.007_bib25","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1093\/infdis\/jis307","article-title":"Nontypeable pneumococcal isolates among Navajo and white mountain Apache communities: are these really a cause of invasive disease?","volume":"206","author":"Scott","year":"2012","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.jiac.2019.02.007_bib26","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0092-8674(00)00071-4","article-title":"The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells","volume":"102","author":"Zhang","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.jiac.2019.02.007_bib27","series-title":"Manual of clinical microbiology","first-page":"1314","article-title":"Susceptibility test methods: fastidious bacteria","author":"Humphries","year":"2015"},{"key":"10.1016\/j.jiac.2019.02.007_bib28","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1128\/AAC.40.10.2252","article-title":"ParC subunit of DNA topoisomerase IV of Streptococcus pneumoniae is a primary target of fluoroquinolones and cooperates with DNA gyrase A subunit in forming resistance phenotype","volume":"40","author":"Mu\u00f1oz","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib29","doi-asserted-by":"crossref","first-page":"4060","DOI":"10.1128\/jb.178.14.4060-4069.1996","article-title":"Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in fluoroquinolone resistance","volume":"178","author":"Pan","year":"1996","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.jiac.2019.02.007_bib30","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1093\/jac\/dkg309","article-title":"Cross-resistance, relatedness and allele analysis of fluoroquinolone-resistant US clinical isolates of Streptococcus pneumoniae (1998-2000)","volume":"52","author":"Davies","year":"2003","journal-title":"J\u00a0Antimicrob Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1089\/107662904323047781","article-title":"Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study","volume":"10","author":"Oh","year":"2004","journal-title":"Microb Drug Resist"},{"key":"10.1016\/j.jiac.2019.02.007_bib32","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.3201\/eid2111.142029","article-title":"Invasive Pneumococcal Diseases Surveillance Study Group. Serotype changes and drug resistance in invasive pneumococcal diseases in adults after vaccinations in children, Japan, 2010-2013","volume":"21","author":"Ubukata","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jiac.2019.02.007_bib33","doi-asserted-by":"crossref","first-page":"6178","DOI":"10.1016\/j.vaccine.2015.10.015","article-title":"Invasive disease potential of pneumococci before and after the 13-valent pneumococcal conjugate vaccine implementation in children","volume":"33","author":"Varon","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.jiac.2019.02.007_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-015-0978-9","article-title":"Trends in antibiotic resistance of Streptococcus pneumoniae and Haemophilus influenzae isolated from nasopharyngeal flora in children with acute otitis media in France before and after 13 valent pneumococcal conjugate vaccine introduction","author":"Angoulvant","year":"2015","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.jiac.2019.02.007_bib35","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/mmi.13721","article-title":"A\u00a0novel streptococcal cell-cell communication peptide promotes pneumococcal virulence and biofilm formation","volume":"105","author":"Cuevas","year":"2017","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.jiac.2019.02.007_bib36","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1128\/JB.01369-06","article-title":"Characterization of colony morphology variants isolated from Streptococcus pneumoniae biofilms","volume":"189","author":"Allegrucci","year":"2007","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.jiac.2019.02.007_bib37","doi-asserted-by":"crossref","first-page":"7785","DOI":"10.1128\/JB.00673-06","article-title":"Biofilm formation by Streptococcus pneumoniae: role of choline, extracellular DNA, and capsular polysaccharide in microbial accretion","volume":"188","author":"Moscoso","year":"2006","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.jiac.2019.02.007_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001108","article-title":"Generation of genetic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection","volume":"6","author":"Hiller","year":"2010","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.jiac.2019.02.007_bib39","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jiac.2013.06.001","article-title":"The relationship between biofilm formations and capsule in Haemophilus influenzae","volume":"20","author":"Qin","year":"2014","journal-title":"J\u00a0Infect Chemother"},{"key":"10.1016\/j.jiac.2019.02.007_bib40","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftu011","article-title":"Nonencapsulated Streptococcus pneumoniae causes otitis media during single-species infection and during polymicrobial infection with nontypeable Haemophilus influenzae","author":"Murrah","year":"2015","journal-title":"Pathog Dis"}],"container-title":["Journal of Infection and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1341321X19300388?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1341321X19300388?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:58:45Z","timestamp":1761159525000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1341321X19300388"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":40,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["S1341321X19300388"],"URL":"https:\/\/doi.org\/10.1016\/j.jiac.2019.02.007","relation":{},"ISSN":["1341-321X"],"issn-type":[{"value":"1341-321X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular typing, antibiotic susceptibility, and biofilm production in nonencapsulated Streptococcus pneumoniae isolated from children in Japan","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection and Chemotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jiac.2019.02.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}