{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:36:37Z","timestamp":1788348997383,"version":"build-2803163510"},"reference-count":44,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["jidonline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Dermatology"],"published-print":{"date-parts":[[2018,12]]},"DOI":"10.1016\/j.jid.2018.06.169","type":"journal-article","created":{"date-parts":[[2018,6,30]],"date-time":"2018-06-30T03:33:16Z","timestamp":1530329596000},"page":"2558-2567","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Detection of HPV E7 Transcription at\u00a0Single-Cell Resolution in Epidermis"],"prefix":"10.1016","volume":"138","author":[{"given":"Samuel W.","family":"Lukowski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zewen K.","family":"Tuong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Noske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Senabouth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan H.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey B.","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Peter","family":"Soyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian H.","family":"Frazer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph E.","family":"Powell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jid.2018.06.169_bib1","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1038\/jid.2011.91","article-title":"Transcriptome sequencing demonstrates that human papillomavirus is not active in cutaneous squamous cell carcinoma","volume":"131","author":"Arron","year":"2011","journal-title":"J\u00a0Invest Dermatol"},{"key":"10.1016\/j.jid.2018.06.169_bib2","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1002\/cncr.29976","article-title":"Detection of human papillomavirus type 16 in oropharyngeal squamous cell carcinoma using droplet digital polymerase chain reaction","volume":"122","author":"Biron","year":"2016","journal-title":"Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib3","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1093\/carcin\/bgi123","article-title":"Non-melanoma skin cancer: what drives tumor development and progression?","volume":"26","author":"Boukamp","year":"2005","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.jid.2018.06.169_bib4","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/JCM.37.7.2270-2273.1999","article-title":"Detection of human papillomavirus types 6 and 11 in pubic and perianal hair from patients with genital warts","volume":"37","author":"Boxman","year":"1999","journal-title":"J\u00a0Clin Microbiol"},{"issue":"2","key":"10.1016\/j.jid.2018.06.169_bib5","doi-asserted-by":"crossref","first-page":"e57798","DOI":"10.1371\/journal.pone.0057798","article-title":"Expression of a single, viral oncoprotein in skin epithelium is sufficient to recruit lymphocytes","volume":"8","author":"Choyce","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jid.2018.06.169_bib6","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jid.2018.06.169_bib7","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1002\/ijc.28176","article-title":"Cutaneous alpha, beta and gamma human papillomaviruses in relation to squamous cell carcinoma of the skin: a population-based study","volume":"133","author":"Farzan","year":"2013","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib8","first-page":"201","article-title":"Progression of actinic keratosis to squamous cell carcinoma revisited: clinical and treatment implications","volume":"87","author":"Feldman","year":"2011","journal-title":"Cutis"},{"key":"10.1016\/j.jid.2018.06.169_bib9","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/(SICI)1097-0215(19960703)67:1<24::AID-IJC6>3.0.CO;2-1","article-title":"Site distribution of different types of skin cancer: new aetiological clues","volume":"67","author":"Franceschi","year":"1996","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib10","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1038\/jid.2014.456","article-title":"The presence of betapapillomavirus antibodies around transplantation predicts the development of keratinocyte carcinoma in organ transplant recipients: a cohort study","volume":"135","author":"Genders","year":"2015","journal-title":"J\u00a0Invest Dermatol"},{"key":"10.1016\/j.jid.2018.06.169_bib11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1097\/QCO.0b013e3282f486c9","article-title":"Epidermodysplasia verruciformis and human papilloma virus","volume":"21","author":"Gewirtzman","year":"2008","journal-title":"Curr Opin Infect Dis"},{"key":"10.1016\/j.jid.2018.06.169_bib12","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/1753-6405.12738","article-title":"Multiplicity of skin cancers in Queensland and their cost burden to government and patients","volume":"42","author":"Gordon","year":"2017","journal-title":"Aust NZ J Public Health"},{"key":"10.1016\/j.jid.2018.06.169_bib13","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1128\/JVI.70.3.1873-1881.1996","article-title":"Squamous epithelial hyperplasia and carcinoma in mice transgenic for the human papillomavirus type 16 E7 oncogene","volume":"70","author":"Herber","year":"1996","journal-title":"J\u00a0Virol"},{"key":"10.1016\/j.jid.2018.06.169_bib14","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1097\/01.MP.0000032536.48264.D1","article-title":"Immunohistochemical comparison of p16 expression in actinic keratoses and squamous cell carcinomas of the skin","volume":"15","author":"Hodges","year":"2002","journal-title":"Mod Pathol"},{"key":"10.1016\/j.jid.2018.06.169_bib15","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1002\/ijc.28552","article-title":"Case-control study of genus-beta human papillomaviruses in plucked eyebrow hairs and cutaneous squamous cell carcinoma","volume":"134","author":"Iannacone","year":"2014","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib16","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.cels.2016.08.010","article-title":"Single-cell transcriptomics reveals that differentiation and spatial signatures shape epidermal and hair follicle heterogeneity","volume":"3","author":"Joost","year":"2016","journal-title":"Cell Syst"},{"issue":"6","key":"10.1016\/j.jid.2018.06.169_bib17","doi-asserted-by":"crossref","first-page":"e1003432","DOI":"10.1371\/journal.ppat.1003432","article-title":"Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule","volume":"9","author":"Josefsson","year":"2013","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.jid.2018.06.169_bib18","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1101\/gr.192237.115","article-title":"Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells","volume":"25","author":"Kowalczyk","year":"2015","journal-title":"Genome Res"},{"key":"10.1016\/j.jid.2018.06.169_bib19","doi-asserted-by":"crossref","first-page":"5583","DOI":"10.1073\/pnas.90.12.5583","article-title":"Epidermal cancer associated with expression of human papillomavirus type 16 E6 and E7 oncogenes in the skin of transgenic mice","volume":"90","author":"Lambert","year":"1993","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jid.2018.06.169_bib20","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1111\/bjd.15931","article-title":"Epidemiology of keratinocyte carcinomas after organ transplantation","volume":"177","author":"Madeleine","year":"2017","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.jid.2018.06.169_bib21","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.4049\/jimmunol.0902191","article-title":"Invariant NKT cells in hyperplastic skin induce a local immune suppressive environment by IFN-gamma production","volume":"184","author":"Mattarollo","year":"2010","journal-title":"J\u00a0Immunol"},{"issue":"4","key":"10.1016\/j.jid.2018.06.169_bib22","doi-asserted-by":"crossref","first-page":"e1006211","DOI":"10.1371\/journal.ppat.1006211","article-title":"The role of integration in oncogenic progression of HPV-associated cancers","volume":"13","author":"McBride","year":"2017","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.jid.2018.06.169_bib23","doi-asserted-by":"crossref","first-page":"12648","DOI":"10.1128\/JVI.01602-13","article-title":"Determining the cellular diversity of hepatitis C virus quasispecies by single-cell viral sequencing","volume":"87","author":"McWilliam Leitch","year":"2013","journal-title":"J\u00a0Virol"},{"key":"10.1016\/j.jid.2018.06.169_bib24","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s00418-008-0435-6","article-title":"The human keratins: biology and pathology","volume":"129","author":"Moll","year":"2008","journal-title":"Histochem Cell Biol"},{"key":"10.1016\/j.jid.2018.06.169_bib25","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1158\/1055-9965.EPI-12-0917-T","article-title":"Human papillomavirus load in eyebrow hair follicles and risk of cutaneous squamous cell carcinoma","volume":"22","author":"Neale","year":"2013","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"10.1016\/j.jid.2018.06.169_bib26","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1476-4598-5-30","article-title":"Identification of differentially expressed genes in cutaneous squamous cell carcinoma by microarray expression profiling","volume":"5","author":"Nindl","year":"2006","journal-title":"Mol Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat Methods"},{"issue":"31","key":"10.1016\/j.jid.2018.06.169_bib28","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/oxfordjournals.jncimonographs.a003483","article-title":"Chapter 8: human papillomavirus and skin cancer","volume":"2003","author":"Pfister","year":"2003","journal-title":"J\u00a0Natl Cancer Inst Monogr"},{"key":"10.1016\/j.jid.2018.06.169_bib29","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1086\/597619","article-title":"Anogenital hairs are an important reservoir of alpha-papillomaviruses in patients with genital warts","volume":"199","author":"Poljak","year":"2009","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.jid.2018.06.169_bib30","first-page":"4862","article-title":"Dissection of human papillomavirus E6 and E7 function in transgenic mouse models of cervical carcinogenesis","volume":"63","author":"Riley","year":"2003","journal-title":"Cancer Res"},{"key":"10.1016\/j.jid.2018.06.169_bib31","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jid.2018.06.169_bib32","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jid.2018.06.169_bib33","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13000-016-0505-3","article-title":"Human papillomavirus infection and p16 expression in the immunocompetent patients with extragenital\/extraungual Bowen\u2019s disease","volume":"11","author":"Svajdler","year":"2016","journal-title":"Diagn Pathol"},{"key":"10.1016\/j.jid.2018.06.169_bib34","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3322\/caac.21262","article-title":"Global cancer statistics, 2012","volume":"65","author":"Torre","year":"2015","journal-title":"CA Cancer J Clin"},{"issue":"6","key":"10.1016\/j.jid.2018.06.169_bib35","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.pvr.2017.10.001","article-title":"Murine HPV16 E7-expressing transgenic skin effectively emulates the cellular and molecular features of human high-grade squamous intraepithelial lesions","volume":"5","author":"Tuong","year":"2018","journal-title":"Papillomavirus Res"},{"issue":"D1","key":"10.1016\/j.jid.2018.06.169_bib36","doi-asserted-by":"crossref","first-page":"D499","DOI":"10.1093\/nar\/gkw879","article-title":"The Papillomavirus Episteme: a major update to the papillomavirus sequence database","volume":"45","author":"Van Doorslaer","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jid.2018.06.169_bib37","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1140\/epjb\/e2013-40829-0","article-title":"A\u00a0smart local moving algorithm for large-scale modularity-based community detection","volume":"86","author":"Waltman","year":"2013","journal-title":"Eur Phys J B"},{"key":"10.1016\/j.jid.2018.06.169_bib38","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1002\/cncr.10439","article-title":"Quantitation of human papillomavirus 16 E6 and E7 DNA and RNA in residual material from ThinPrep Papanicolaou tests using real-time polymerase chain reaction analysis","volume":"94","author":"Wang-Johanning","year":"2002","journal-title":"Cancer"},{"key":"10.1016\/j.jid.2018.06.169_bib39","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1159\/000366536","article-title":"Clinical features of actinic keratoses and early squamous cell carcinoma","volume":"46","author":"Wheller","year":"2015","journal-title":"Curr Probl Dermatol"},{"issue":"18","key":"10.1016\/j.jid.2018.06.169_bib40","doi-asserted-by":"crossref","first-page":"E3659","DOI":"10.1073\/pnas.1617961114","article-title":"Single-cell analysis of HIV-1 transcriptional activity reveals expression of proviruses in expanded clones during ART","volume":"114","author":"Wiegand","year":"2017","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jid.2018.06.169_bib41","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s13742-015-0091-4","article-title":"Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells","volume":"4","author":"Wu","year":"2015","journal-title":"GigaScience"},{"key":"10.1016\/j.jid.2018.06.169_bib42","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.jaad.2011.02.011","article-title":"Dermatoscopy of facial actinic keratosis, intraepidermal carcinoma, and invasive squamous cell carcinoma: a progression model","volume":"66","author":"Zalaudek","year":"2012","journal-title":"J\u00a0Am Acad Dermatol"},{"key":"10.1016\/j.jid.2018.06.169_bib43","doi-asserted-by":"crossref","first-page":"14049","DOI":"10.1038\/ncomms14049","article-title":"Massively parallel digital transcriptional profiling of single cells","volume":"8","author":"Zheng","year":"2017","journal-title":"Nat Commun"},{"key":"10.1016\/j.jid.2018.06.169_bib44","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.ebiom.2016.06.011","article-title":"A\u00a0mouse model of hyperproliferative human epithelium validated by keratin profiling shows an aberrant cytoskeletal response to injury","volume":"9","author":"Zhussupbekova","year":"2016","journal-title":"EBioMedicine"}],"container-title":["Journal of Investigative Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022202X18322292?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022202X18322292?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T15:28:35Z","timestamp":1604503715000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022202X18322292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":44,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["S0022202X18322292"],"URL":"https:\/\/doi.org\/10.1016\/j.jid.2018.06.169","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/252858","asserted-by":"object"}]},"ISSN":["0022-202X"],"issn-type":[{"value":"0022-202X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of HPV E7 Transcription at\u00a0Single-Cell Resolution in Epidermis","name":"articletitle","label":"Article Title"},{"value":"Journal of Investigative Dermatology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jid.2018.06.169","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology.","name":"copyright","label":"Copyright"}]}}