{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:51:00Z","timestamp":1786992660825,"version":"build-2736575974"},"reference-count":46,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["F31 AR075436-01"],"award-info":[{"award-number":["F31 AR075436-01"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["P30-AR069589"],"award-info":[{"award-number":["P30-AR069589"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01 EY024704"],"award-info":[{"award-number":["R01 EY024704"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI125743"],"award-info":[{"award-number":["R01AI125743"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI163146"],"award-info":[{"award-number":["R01AI163146"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS083702"],"award-info":[{"award-number":["R01 NS083702"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS094224"],"award-info":[{"award-number":["R01NS094224"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jidonline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Dermatology"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.jid.2021.08.433","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T01:36:19Z","timestamp":1632706579000},"page":"1136-1144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["TRPC3 Antagonizes Pruritus in a Mouse Contact Dermatitis Model"],"prefix":"10.1016","volume":"142","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1055-4547","authenticated-orcid":false,"given":"Katherine","family":"Beattie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2477-5970","authenticated-orcid":false,"given":"Haowu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7257-5837","authenticated-orcid":false,"given":"Mayank","family":"Gautam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4794-643X","authenticated-orcid":false,"given":"Mary K.","family":"MacVittie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9313-4686","authenticated-orcid":false,"given":"Barbara","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5027-9623","authenticated-orcid":false,"given":"Minghong","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4333-4951","authenticated-orcid":false,"given":"Qin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2486-807X","authenticated-orcid":false,"given":"Wenqin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jid.2021.08.433_bib1","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1016\/j.celrep.2019.07.017","article-title":"Development of a mouse pain scale using sub-second behavioral mapping and statistical modeling","volume":"28","author":"Abdus-Saboor","year":"2019","journal-title":"Cell Rep"},{"key":"10.1016\/j.jid.2021.08.433_bib2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1515\/reveh-2014-0060","article-title":"Irritant contact dermatitis","volume":"29","author":"Ale","year":"2014","journal-title":"Rev Environ Health"},{"key":"10.1016\/j.jid.2021.08.433_bib3","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/1744-8069-10-43","article-title":"Contribution of TRPC3 to store-operated calcium entry and inflammatory transductions in primary nociceptors","volume":"10","author":"Alkhani","year":"2014","journal-title":"Mol Pain"},{"key":"10.1016\/j.jid.2021.08.433_bib4","doi-asserted-by":"crossref","first-page":"6706","DOI":"10.1073\/pnas.0810599106","article-title":"A\u00a0point mutation in TRPC3 causes abnormal Purkinje cell development and cerebellar ataxia in moonwalker mice","volume":"106","author":"Becker","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jid.2021.08.433_bib5","doi-asserted-by":"crossref","first-page":"5067","DOI":"10.1523\/JNEUROSCI.6451-10.2011","article-title":"Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells","volume":"31","author":"Cavanaugh","year":"2011","journal-title":"J\u00a0Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib6","doi-asserted-by":"crossref","first-page":"9075","DOI":"10.1073\/pnas.0901507106","article-title":"Distinct subsets of unmyelinated primary sensory fibers mediate behavioral responses to noxious thermal and mechanical stimuli [published correction appears in Proc Natl Acad Sci USA 2009;106:11424]","volume":"106","author":"Cavanaugh","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jid.2021.08.433_bib7","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.3390\/cells9091983","article-title":"Transient receptor potential canonical (TRPC) channels: then and now","volume":"9","author":"Chen","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.jid.2021.08.433_bib8","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/nn.4549","article-title":"Identification of spinal circuits involved in touch-evoked dynamic mechanical pain","volume":"20","author":"Cheng","year":"2017","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/bs.ctdb.2020.10.005","article-title":"The development of somatosensory neurons: insights into pain and itch","volume":"142","author":"Cranfill","year":"2021","journal-title":"Curr Top Dev Biol"},{"key":"10.1016\/j.jid.2021.08.433_bib10","doi-asserted-by":"crossref","first-page":"13869","DOI":"10.1038\/s41598-017-12770-0","article-title":"TRPC3 is dispensable for \u03b2-alanine triggered acute itch","volume":"7","author":"Dong","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.jid.2021.08.433_bib11","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/s41467-017-01056-8","article-title":"Sensory TRP channels contribute differentially to skin inflammation and persistent itch","volume":"8","author":"Feng","year":"2017","journal-title":"Nat Commun"},{"key":"10.1016\/j.jid.2021.08.433_bib12","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/nn.3289","article-title":"A\u00a0subpopulation of nociceptors specifically linked to itch","volume":"16","author":"Han","year":"2013","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib13","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.neuron.2008.06.009","article-title":"TRPC3 channels are required for synaptic transmission and motor coordination","volume":"59","author":"Hartmann","year":"2008","journal-title":"Neuron"},{"key":"10.1016\/j.jid.2021.08.433_bib14","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.exphem.2011.09.006","article-title":"Trpc2 depletion protects red blood cells from oxidative stress-induced hemolysis","volume":"40","author":"Hirschler-Laszkiewicz","year":"2012","journal-title":"Exp Hematol"},{"key":"10.1016\/j.jid.2021.08.433_bib15","doi-asserted-by":"crossref","first-page":"618663","DOI":"10.3389\/fcell.2020.618663","article-title":"Contribution of TRPC channels in neuronal excitotoxicity associated with neurodegenerative disease and ischemic stroke","volume":"8","author":"Jeon","year":"2020","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.jid.2021.08.433_bib16","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.stem.2020.01.012","article-title":"The molecular anatomy of mouse skin during hair growth and rest","volume":"26","author":"Joost","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.jid.2021.08.433_bib17","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev-cellbio-101011-155833","article-title":"TRP channels and pain","volume":"29","author":"Julius","year":"2013","journal-title":"Annu Rev Cell Dev Biol"},{"key":"10.1016\/j.jid.2021.08.433_bib18","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.iac.2016.08.014","article-title":"Allergic contact dermatitis","volume":"37","author":"Kostner","year":"2017","journal-title":"Immunol Allergy Clin North Am"},{"key":"10.1016\/j.jid.2021.08.433_bib19","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.neuron.2010.09.016","article-title":"VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch","volume":"68","author":"Lagerstr\u00f6m","year":"2010","journal-title":"Neuron"},{"key":"10.1016\/j.jid.2021.08.433_bib20","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/72101","article-title":"Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1","volume":"3","author":"Lin","year":"2000","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib21","doi-asserted-by":"crossref","first-page":"14532","DOI":"10.1523\/JNEUROSCI.3509-12.2012","article-title":"Mechanisms of itch evoked by \u03b2-alanine","volume":"32","author":"Liu","year":"2012","journal-title":"J\u00a0Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib22","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1016\/j.cell.2009.11.034","article-title":"Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus","volume":"139","author":"Liu","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.jid.2021.08.433_bib23","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.neuron.2010.09.008","article-title":"VGLUT2-dependent glutamate release from nociceptors is required to sense pain and suppress itch","volume":"68","author":"Liu","year":"2010","journal-title":"Neuron"},{"key":"10.1016\/j.jid.2021.08.433_bib24","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.neuron.2009.11.003","article-title":"Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling","volume":"64","author":"Luo","year":"2009","journal-title":"Neuron"},{"key":"10.1016\/j.jid.2021.08.433_bib25","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1126\/science.1233765","article-title":"The cells and circuitry for itch responses in mice","volume":"340","author":"Mishra","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.jid.2021.08.433_bib26","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s00424-010-0920-3","article-title":"The history of TRP channels, a commentary and reflection","volume":"461","author":"Montell","year":"2011","journal-title":"Pflugers Arch"},{"key":"10.1016\/j.jid.2021.08.433_bib27","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1684\/ejd.2009.0686","article-title":"Allergic and irritant contact dermatitis","volume":"19","author":"Nosbaum","year":"2009","journal-title":"Eur J Dermatol"},{"key":"10.1016\/j.jid.2021.08.433_bib28","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.29507","article-title":"Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors","volume":"6","author":"Olson","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.jid.2021.08.433_bib29","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1093\/brain\/awu007","article-title":"Enhanced excitability of MRGPRA3- and MRGPRD-positive nociceptors in a model of inflammatory itch and pain","volume":"137","author":"Qu","year":"2014","journal-title":"Brain"},{"key":"10.1016\/j.jid.2021.08.433_bib30","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1097\/j.pain.0000000000000208","article-title":"CXCR3 chemokine receptor signaling mediates itch in experimental allergic contact dermatitis","volume":"156","author":"Qu","year":"2015","journal-title":"Pain"},{"key":"10.1016\/j.jid.2021.08.433_bib31","doi-asserted-by":"crossref","first-page":"9554","DOI":"10.1523\/JNEUROSCI.6355-11.2012","article-title":"Transient receptor potential canonical 3 (TRPC3) is required for IgG immune complex-induced excitation of the rat dorsal root ganglion neurons","volume":"32","author":"Qu","year":"2012","journal-title":"J\u00a0Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib32","doi-asserted-by":"crossref","first-page":"120068","DOI":"10.1098\/rsob.120068","article-title":"TRPC3 and TRPC6 are essential for normal mechanotransduction in subsets of sensory neurons and cochlear hair cells","volume":"2","author":"Quick","year":"2012","journal-title":"Open Biol"},{"key":"10.1016\/j.jid.2021.08.433_bib33","series-title":"Pharmacology of itch","first-page":"177","article-title":"Interactions between keratinocytes and somatosensory neurons in itch","author":"Schwendinger-Schreck","year":"2015"},{"key":"10.1016\/j.jid.2021.08.433_bib34","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1006\/taap.2002.9469","article-title":"Insights into the quantitative relationship between sensitization and challenge for allergic contact dermatitis reactions","volume":"183","author":"Scott","year":"2002","journal-title":"Toxicol Appl Pharmacol"},{"key":"10.1016\/j.jid.2021.08.433_bib35","doi-asserted-by":"crossref","first-page":"23559","DOI":"10.1074\/jbc.M314240200","article-title":"Identification of a G protein-coupled receptor specifically responsive to \u03b2-alanine","volume":"279","author":"Shinohara","year":"2004","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.jid.2021.08.433_bib36","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1016\/j.celrep.2019.02.089","article-title":"Nppb neurons are sensors of mast cell-induced itch","volume":"26","author":"Solinski","year":"2019","journal-title":"Cell Rep"},{"key":"10.1016\/j.jid.2021.08.433_bib37","series-title":"Pharmacology of itch","first-page":"163","article-title":"Role of cytokines and chemokines in itch","author":"Storan","year":"2015"},{"key":"10.1016\/j.jid.2021.08.433_bib38","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s00281-015-0530-4","article-title":"Trp channels and itch","volume":"38","author":"Sun","year":"2016","journal-title":"Semin Immunopathol"},{"key":"10.1016\/j.jid.2021.08.433_bib39","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/nn.3881","article-title":"Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing","volume":"18","author":"Usoskin","year":"2015","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jid.2021.08.433_bib40","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.cell.2020.12.033","article-title":"A\u00a0basophil-neuronal axis promotes itch","volume":"184","author":"Wang","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.jid.2021.08.433_bib41","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.bbamcr.2010.12.022","article-title":"TRP expression pattern and the functional importance of TRPC3 in primary human T-cells","volume":"1813","author":"Wenning","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.jid.2021.08.433_bib42","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jim.2008.06.001","article-title":"Transgenic tools for analysis of the haematopoietic system: knock-in CD45 reporter and deletor mice","volume":"337","author":"Yang","year":"2008","journal-title":"J\u00a0Immunol Methods"},{"key":"10.1016\/j.jid.2021.08.433_bib43","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cell.2018.06.021","article-title":"Molecular architecture of the mouse nervous system","volume":"174","author":"Zeisel","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.jid.2021.08.433_bib44","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1016\/j.cell.2021.03.002","article-title":"Nonpeptidergic neurons suppress mast cells via glutamate to maintain skin homeostasis","volume":"184","author":"Zhang","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.jid.2021.08.433_bib45","first-page":"e9","article-title":"LMrgprs activation is required for chronic itch conditions in mice","volume":"2","author":"Zhu","year":"2017","journal-title":"Itch (Phila)"},{"key":"10.1016\/j.jid.2021.08.433_bib46","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.neuron.2004.12.015","article-title":"Topographically distinct epidermal nociceptive circuits revealed by axonal tracers targeted to Mrgprd","volume":"45","author":"Zylka","year":"2005","journal-title":"Neuron"}],"container-title":["Journal of Investigative Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022202X21022223?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022202X21022223?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T17:26:58Z","timestamp":1758302818000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022202X21022223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["S0022202X21022223"],"URL":"https:\/\/doi.org\/10.1016\/j.jid.2021.08.433","relation":{},"ISSN":["0022-202X"],"issn-type":[{"value":"0022-202X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"TRPC3 Antagonizes Pruritus in a Mouse Contact Dermatitis Model","name":"articletitle","label":"Article Title"},{"value":"Journal of Investigative Dermatology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jid.2021.08.433","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology.","name":"copyright","label":"Copyright"}]}}