{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:16:27Z","timestamp":1778915787655,"version":"3.51.4"},"reference-count":19,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2004,6]]},"DOI":"10.1016\/j.jim.2004.04.015","type":"journal-article","created":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T20:57:06Z","timestamp":1086209826000},"page":"179-190","source":"Crossref","is-referenced-by-count":21,"title":["Human tonsillar tissue block cultures differ from autologous tonsillar cell suspension cultures in lymphocyte subset activation and cytokine gene expression"],"prefix":"10.1016","volume":"289","author":[{"given":"Bettina","family":"Giger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athos","family":"Bonanomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Odermatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin","family":"Ladell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto F","family":"Speck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dejan","family":"Kojic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Berger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix K","family":"Niggli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Nadal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2004.04.015_BIB1","series-title":"Cellular and Molecular Immunology","author":"Abbas","year":"2003"},{"key":"10.1016\/j.jim.2004.04.015_BIB2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0168-3659(01)00488-6","article-title":"Clinical aspects of drug delivery to tumors","volume":"78","author":"Au","year":"2002","journal-title":"J. Control. Release"},{"key":"10.1016\/j.jim.2004.04.015_BIB3","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1097\/00002030-200004140-00003","article-title":"HIV-infected human Langerhans cells transmit infection to human lymphoid tissue ex vivo","volume":"14","author":"Blauvelt","year":"2000","journal-title":"AIDS"},{"key":"10.1016\/j.jim.2004.04.015_BIB4","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jim.2003.08.002","article-title":"Quantitative cytokine gene expression in human tonsils at excision and during histoculture assessed by standardized and calibrated real-time PCR and novel data processing","volume":"283","author":"Bonanomi","year":"2003","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2004.04.015_BIB5","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1128\/JVI.76.3.1004-1014.2002","article-title":"Presence of host ICAM-1 in laboratory and clinical strains of human immunodeficiency virus type 1 increases virus infectivity and CD4(+)-T-cell depletion in human lymphoid tissue, a major site of replication in vivo","volume":"76","author":"Bounou","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.jim.2004.04.015_BIB6","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/74743","article-title":"Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract","volume":"6","author":"Collins","year":"2000","journal-title":"Nat. Med."},{"key":"10.1016\/j.jim.2004.04.015_BIB7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0171-2985(11)80486-9","article-title":"An organ fragment culture model to study lymphocyte activation in human lymphoid tissue","volume":"188","author":"Ferro","year":"1993","journal-title":"Immunobiology"},{"key":"10.1016\/j.jim.2004.04.015_BIB8","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1038\/nm1295-1320","article-title":"Infection of human tonsil histocultures: a model for HIV pathogenesis","volume":"1","author":"Glushakova","year":"1995","journal-title":"Nat. Med."},{"key":"10.1016\/j.jim.2004.04.015_BIB9","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1038\/nm1101-1232","article-title":"Suppression of CCR5\u2014but not CXCR4\u2014tropic HIV-1 in lymphoid tissue by human herpesvirus 6","volume":"7","author":"Grivel","year":"2001","journal-title":"Nat. Med."},{"key":"10.1016\/j.jim.2004.04.015_BIB10","doi-asserted-by":"crossref","first-page":"8280","DOI":"10.1128\/JVI.77.15.8280-8289.2003","article-title":"Pathogenic effects of human herpesvirus 6 in human lymphoid tissue ex vivo","volume":"77","author":"Grivel","year":"2003","journal-title":"J. Virol."},{"key":"10.1016\/j.jim.2004.04.015_BIB11","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0022-1759(94)00268-2","article-title":"Organ culture of human lymphoid tissue: I. Characteristics of the system","volume":"179","author":"Hoffmann","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2004.04.015_BIB12","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1002\/eji.1830120710","article-title":"Effect of deoxyguanosine on lymphopoiesis in the developing thymus rudiment in vitro: application in the production of chimeric thymus rudiments","volume":"12","author":"Jenkinson","year":"1982","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jim.2004.04.015_BIB13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0167-5699(92)90199-H","article-title":"Germinal centres in T-cell-dependent antibody responses","volume":"13","author":"Liu","year":"1992","journal-title":"Immunol. Today"},{"key":"10.1016\/j.jim.2004.04.015_BIB14","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0162-3109(00)00192-2","article-title":"Mechanisms of action of cyclosporine","volume":"47","author":"Matsuda","year":"2000","journal-title":"Immunopharmacology"},{"key":"10.1016\/j.jim.2004.04.015_BIB15","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1089\/08892220151126580","article-title":"Primary and recombinant HIV type 1 strains resistant to protease inhibitors are pathogenic in mature human lymphoid tissues","volume":"17","author":"Penn","year":"2001","journal-title":"AIDS Res. Hum. Retrovir."},{"key":"10.1016\/j.jim.2004.04.015_BIB16","doi-asserted-by":"crossref","first-page":"11205","DOI":"10.1073\/pnas.92.24.11205","article-title":"Immunosuppressive drugs prevent a rapid dephosphorylation of transcription factor NFAT1 in stimulated immune cells","volume":"92","author":"Shaw","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jim.2004.04.015_BIB18","first-page":"411","article-title":"The use of tissue slices in immunological investigations","volume":"45","author":"Skibinski","year":"1997","journal-title":"Arch. Immunol. Ther. Exp. (Warsz)"},{"key":"10.1016\/j.jim.2004.04.015_BIB17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0022-1759(97)00058-6","article-title":"Organ culture of human lymphoid tissue: II. Marked differences in cytokine production and proliferation between slice and suspension cultures of human spleen","volume":"205","author":"Skibinski","year":"1997","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2004.04.015_BIB19","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1046\/j.1365-2567.2003.01585.x","article-title":"Immunomodulatory effects of cyclosporin A on human peripheral blood dendritic cell subsets","volume":"108","author":"Tajima","year":"2003","journal-title":"Immunology"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175904001528?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175904001528?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T13:27:54Z","timestamp":1549546074000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175904001528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":19,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["S0022175904001528"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2004.04.015","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}