{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T22:17:23Z","timestamp":1774909043107,"version":"3.50.1"},"reference-count":28,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100007048","name":"Mayo Foundation for Medical Education and Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007048","id-type":"DOI","asserted-by":"crossref"}]},{"name":"United States National Institutes of Health","award":["HL058723"],"award-info":[{"award-number":["HL058723"]}]},{"name":"United States National Institutes of Health","award":["HL065228"],"award-info":[{"award-number":["HL065228"]}]},{"name":"United States National Institutes of Health","award":["RR0109709"],"award-info":[{"award-number":["RR0109709"]}]},{"name":"United States National Institutes of Health","award":["HL068546"],"award-info":[{"award-number":["HL068546"]}]},{"name":"United States National Institutes of Health","award":["HL078860"],"award-info":[{"award-number":["HL078860"]}]},{"name":"United States National Institutes of Health","award":["HL088594"],"award-info":[{"award-number":["HL088594"]}]},{"name":"United States National Institutes of Health","award":["RR025011"],"award-info":[{"award-number":["RR025011"]}]},{"name":"United States National Institutes of Health","award":["HL56396"],"award-info":[{"award-number":["HL56396"]}]},{"name":"United States National Institutes of Health","award":["RR03186"],"award-info":[{"award-number":["RR03186"]}]},{"name":"United States National Institutes of Health","award":["RR025780"],"award-info":[{"award-number":["RR025780"]}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["05556392"],"award-info":[{"award-number":["05556392"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["0855703"],"award-info":[{"award-number":["0855703"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Bazley Trust"},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["MOP89748"],"award-info":[{"award-number":["MOP89748"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Canada Research Chair in Airway Inflammometry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2012,10]]},"DOI":"10.1016\/j.jim.2012.06.011","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:17:09Z","timestamp":1340842629000},"page":"10-20","source":"Crossref","is-referenced-by-count":40,"title":["A sensitive high throughput ELISA for human eosinophil peroxidase: A specific assay to quantify eosinophil degranulation from patient-derived sources"],"prefix":"10.1016","volume":"384","author":[{"given":"Sergei I.","family":"Ochkur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Dongil","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl A.","family":"Protheroe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Colbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel M.","family":"Condjella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Bersoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Helmers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Redwan","family":"Moqbel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige","family":"Lacy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nizar N.","family":"Jarjour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Kern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anju","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert P.","family":"Schleimer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn T.","family":"Furuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parameswaran","family":"Nair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy A.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2012.06.011_bb0005","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1067\/mai.2000.109622","article-title":"A comparison of exhaled nitric oxide and induced sputum as markers of airway inflammation","volume":"106","author":"Berlyne","year":"2000","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0010","first-page":"1","article-title":"Summary health statistics for U.S. children: National Health Interview Survey, 2006","volume":"10","author":"Bloom","year":"2007","journal-title":"Vital Health Stat."},{"key":"10.1016\/j.jim.2012.06.011_bb0015","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/0091-6749(75)90107-4","article-title":"Standardization of bronchial inhalation challenge procedures","volume":"56","author":"Chai","year":"1975","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0020","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.4049\/jimmunol.167.3.1672","article-title":"Extensive eosinophil degranulation and peroxidase-mediated oxidation of airway proteins do not occur in a mouse ovalbumin-challenge model of pulmonary inflammation","volume":"167","author":"Denzler","year":"2001","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0025","doi-asserted-by":"crossref","first-page":"6327","DOI":"10.4049\/jimmunol.0904043","article-title":"Mouse and human eosinophils degranulate in response to platelet-activating factor (PAF) and lysoPAF via a PAF-receptor-independent mechanism: evidence for a novel receptor","volume":"184","author":"Dyer","year":"2010","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0030","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1053\/j.gastro.2007.08.017","article-title":"Eosinophilic esophagitis in children and adults: a systematic review and consensus recommendations for diagnosis and treatment","volume":"133","author":"Furuta","year":"2007","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jim.2012.06.011_bb0035","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1378\/chest.11-0618","article-title":"Point: is measuring sputum eosinophils useful in the management of severe asthma? Yes","volume":"139","author":"Hargreave","year":"2011","journal-title":"Chest"},{"key":"10.1016\/j.jim.2012.06.011_bb0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2008.03.002","article-title":"Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps","volume":"121","author":"Kato","year":"2008","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0045","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1309\/F3FW-J8EB-X913-G1RJ","article-title":"Diagnostic features and differential diagnosis of Churg\u2013Strauss syndrome in the lung. A review","volume":"114","author":"Katzenstein","year":"2000","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.jim.2012.06.011_bb0050","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1189\/jlb.1204697","article-title":"Myeloperoxidase: friend and foe","volume":"77","author":"Klebanoff","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.jim.2012.06.011_bb0055","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.rmed.2006.08.012","article-title":"Eosinophil cationic protein: is it useful in asthma? A systematic review","volume":"101","author":"Koh","year":"2007","journal-title":"Respir. Med."},{"key":"10.1016\/j.jim.2012.06.011_bb0060","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1126\/science.1099472","article-title":"Defining a link with asthma in mice congenitally deficient in eosinophils","volume":"305","author":"Lee","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.jim.2012.06.011_bb0065","doi-asserted-by":"crossref","first-page":"6452","DOI":"10.4049\/jimmunol.169.11.6452","article-title":"Decreased expression of membrane IL-5 receptor alpha on human eosinophils: i. loss of membrane IL-5 receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge","volume":"169","author":"Liu","year":"2002","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0070","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1136\/thorax.57.5.449","article-title":"Cell specific markers for eosinophils and neutrophils in sputum and bronchoalveolar lavage fluid of patients with respiratory conditions and healthy subjects","volume":"57","author":"Metso","year":"2002","journal-title":"Thorax"},{"key":"10.1016\/j.jim.2012.06.011_bb0075","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1159\/000024358","article-title":"Development of a novel enzyme-linked immunosorbent assay for blood and urinary eosinophil-derived neurotoxin: a preliminary study in patients with bronchial asthma","volume":"122","author":"Morioka","year":"2000","journal-title":"Int. Arch. Allergy Immunol."},{"key":"10.1016\/j.jim.2012.06.011_bb0085","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jim.2011.10.002","article-title":"The development of a sensitive and specific ELISA for mouse eosinophil peroxidase: assessment of eosinophil degranulation ex vivo and in models of human disease","volume":"375","author":"Ochkur","year":"2012","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2012.06.011_bb0090","first-page":"656","article-title":"Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy","volume":"54","author":"Peters","year":"1986","journal-title":"Lab. Investig."},{"key":"10.1016\/j.jim.2012.06.011_bb0095","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1183\/09031936.96.09061174","article-title":"Measurement of inflammatory indices in induced sputum: effects of selection of sputum to minimize salivary contamination","volume":"9","author":"Pizzichini","year":"1996","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.jim.2012.06.011_bb0100","first-page":"1","article-title":"Summary health statistics for U.S. adults: National Health Interview Survey, 2006","volume":"10","author":"Pleis","year":"2007","journal-title":"Vital Health Stat."},{"key":"10.1016\/j.jim.2012.06.011_bb0105","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.cgh.2009.03.022","article-title":"A novel histological scoring system to evaluate mucosal biopsies from patients with eosinophilic esophagitis","volume":"7","author":"Protheroe","year":"2009","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.jim.2012.06.011_bb0110","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0022-1759(91)90177-H","article-title":"Detection of eosinophil cationic protein (ECP) by an enzyme-linked immunosorbent assay","volume":"138","author":"Reimert","year":"1991","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2012.06.011_bb0115","unstructured":"Schleimer, R.P., Kato, A. and Kern, R. In press Eosinophils and Chronic Rhinosinusitis. In: J.J. Lee and H. Rosenberg (Eds.) Eosinophils in Health and Disease. Elsevier, Waltham, MA. ISBN-13: 978-0123943859."},{"key":"10.1016\/j.jim.2012.06.011_bb0120","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1056\/NEJMoa043596","article-title":"Use of exhaled nitric oxide measurements to guide treatment in chronic asthma","volume":"352","author":"Smith","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2012.06.011_bb0125","doi-asserted-by":"crossref","first-page":"14859","DOI":"10.1016\/S0021-9258(18)42119-9","article-title":"Eosinophil-derived neurotoxin and human liver ribonuclease. Identity of structure and linkage of neurotoxicity to nuclease activity","volume":"267","author":"Sorrentino","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jim.2012.06.011_bb0130","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1002\/jlb.63.6.715","article-title":"Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes","volume":"63","author":"Sur","year":"1998","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.jim.2012.06.011_bb0135","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/1099-0496(200102)31:2<121::AID-PPUL1019>3.0.CO;2-T","article-title":"Serum levels of eosinophil cationic protein and eosinophils in asthmatic children during a course of prednisolone therapy","volume":"31","author":"Tang","year":"2001","journal-title":"Pediatr. Pulmonol."},{"key":"10.1016\/j.jim.2012.06.011_bb0140","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/1465-9921-12-116","article-title":"Immunodettection of occult eosinophils in lung tissue biopsies may help predict survival of acute lung injury","volume":"12","author":"Willetts","year":"2011","journal-title":"Respir. Res."},{"key":"10.1016\/j.jim.2012.06.011_bb0145","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1097\/00062752-200001000-00010","article-title":"Myeloperoxidase","volume":"7","author":"Winterbourn","year":"2000","journal-title":"Curr. Opin. Hematol."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217591200172X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217591200172X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T19:20:26Z","timestamp":1642620026000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002217591200172X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":28,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["S002217591200172X"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2012.06.011","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}