{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T20:23:39Z","timestamp":1774383819994,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"China and Nantong Social Programs& Technology Innovation","award":["HS2011011"],"award-info":[{"award-number":["HS2011011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2013,1]]},"DOI":"10.1016\/j.jim.2012.11.001","type":"journal-article","created":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T07:31:02Z","timestamp":1352446262000},"page":"228-236","source":"Crossref","is-referenced-by-count":19,"title":["Preparation and characterization of monoclonal antibody specific for copper\u2013chelate complex"],"prefix":"10.1016","volume":"387","author":[{"given":"Fengquan","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Lou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuying","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2012.11.001_bb0005","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0022-1759(87)90187-6","article-title":"Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay","volume":"100","author":"Beatty","year":"1987","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2012.11.001_bb0010","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S1383-5718(00)00049-8","article-title":"Immunoperoxidase detection of 4-aminobiphenyl- and polycyclic aromatic hydrocarbons-DNA adducts in induced sputum of smokers and non-smokers","volume":"468","author":"Besarati Nia","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.jim.2012.11.001_bb0015","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S0956-5663(01)00223-8","article-title":"Antibody-based sensors for heavy metal ions","volume":"16","author":"Blake","year":"2001","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.jim.2012.11.001_bb0020","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/j.envpol.2010.12.023","article-title":"Avoidance tests with Folsomia candida for the assessment of copper contamination in agricultural soils","volume":"159","author":"Boiteau","year":"2011","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.jim.2012.11.001_bb0025","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0022-1759(00)00190-3","article-title":"A quantitative enzyme-linked immunoassay for the detection of 2,6-dichlorobenzamide (BAM), a degradation product of the herbicide dichlobenil","volume":"240","author":"Bruun","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2012.11.001_bb0030","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.chemosphere.2010.12.054","article-title":"Spatial and seasonal variations in atrazine and metolachlor surface water concentrations in Ontario (Canada) using ELISA","volume":"82","author":"Byer","year":"2011","journal-title":"Chemosphere"},{"key":"10.1016\/j.jim.2012.11.001_bb0035","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1006\/abio.1997.2161","article-title":"The quantification of protein amino group by the trinitrobenzenesulfonic acid method: a reexamination","volume":"249","author":"Cayot","year":"1977","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jim.2012.11.001_bb0040","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.snb.2009.02.010","article-title":"New fluorescent and colorimetric chemosensors bearing rhodamine and binaphthyl groups for the detection of Cu2+","volume":"137","author":"Chen","year":"2009","journal-title":"Sensors Actuators B Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0045","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1021\/ac0012905","article-title":"One-step competitive immunoassay for cadmium ions: development and validation for environmental water samples","volume":"73","author":"Darwish","year":"2001","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0050","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/j.ecoenv.2009.04.006","article-title":"Toxicity and bioaccumulation of copper and lead in five marine microalgae","volume":"72","author":"Debelius","year":"2009","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.jim.2012.11.001_bb0055","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1016\/j.foodchem.2010.07.112","article-title":"Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay","volume":"124","author":"Esteve-Turrillas","year":"2011","journal-title":"Food Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0060","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1089\/hyb.1998.17.125","article-title":"New anti-Cu-TETA and anti-Y\u2013DOTA monoclonal antibodies for potential use in the pre-targeted delivery of radiopharmaceuticals to tumor","volume":"17","author":"Feng","year":"1998","journal-title":"Hybridoma"},{"key":"10.1016\/j.jim.2012.11.001_bb0065","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0039-9140(02)00524-6","article-title":"Classification of commercial wines from the Canary Islands (Spain) by chemometric techniques using metallic contents","volume":"59","author":"Frias","year":"2003","journal-title":"Talanta"},{"key":"10.1016\/j.jim.2012.11.001_bb0070","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1016\/j.bios.2011.03.033","article-title":"Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles","volume":"26","author":"Guo","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.jim.2012.11.001_bb0075","series-title":"Using Antibodies: A Laboratory Manual","first-page":"636","author":"Harlow","year":"1988"},{"key":"10.1016\/j.jim.2012.11.001_bb0085","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1021\/es9703943","article-title":"Detection of heavy metals by immunoassay: optimization and validation of a rapid, portable assay for ionic cadmium","volume":"32","author":"Khosraviani","year":"1998","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.jim.2012.11.001_bb0090","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1021\/ac980765d","article-title":"Monoclonal antibody-based ELISAs for part-per-billion determination of polycyclic aromatic hydrocarbons: effects of haptens and formats on sensitivity and specificity","volume":"71","author":"Li","year":"1999","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0095","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0041-008X(03)00247-3","article-title":"Copper, but not cadmium, is acutely toxic for trout hepatocytes: short-term effects on energetics and ion homeostasis","volume":"191","author":"Manzl","year":"2003","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.jim.2012.11.001_bb0100","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1080\/00032719808005248","article-title":"Characterization of a new monoclonal antibody against mercury (II)","volume":"31","author":"Marx","year":"1999","journal-title":"Anal. Lett."},{"key":"10.1016\/j.jim.2012.11.001_bb0105","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1006\/abio.2001.5032","article-title":"Selective and sensitive detection of pectin lyase activity using a colorimetric test: application to the screening of microorganisms possessing pectin lyase activity","volume":"291","author":"Nedjma","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jim.2012.11.001_bb0110","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.talanta.2011.06.052","article-title":"Highly selectively monitoring heavy and transition metal ions by a fluorescent sensor based on dipeptide","volume":"85","author":"Neupane","year":"2011","journal-title":"Talanta"},{"key":"10.1016\/j.jim.2012.11.001_bb0115","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/316265a0","article-title":"Antibodies against metal chelators","volume":"316","author":"Reardan","year":"1985","journal-title":"Nature"},{"key":"10.1016\/j.jim.2012.11.001_bb0120","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jtemb.2010.11.004","article-title":"Risks and benefits of copper in light of new insights of copper homeostasis","volume":"25","author":"Roma\u02dcna","year":"2011","journal-title":"J. Trace Elem. Med. Biol."},{"key":"10.1016\/j.jim.2012.11.001_bb0125","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0022-1759(94)90081-7","article-title":"Quantitation of epsilon-amino group using amino-acids as reference-standards by trinitrobenzene sulfonic-acid\u2014a simple spectrophotometric method for the estimation of hapten to carrier protein ratio","volume":"167","author":"Sashidhar","year":"1994","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2012.11.001_bb0130","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.chemosphere.2008.10.036","article-title":"Copper accumulation and toxicity in fluvial periphyton: the influence of exposure history","volume":"74","author":"Serra","year":"2009","journal-title":"Chemosphere"},{"key":"10.1016\/j.jim.2012.11.001_bb0135","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1021\/bc034158v","article-title":"Synthesis and characterization of hapten\u2013protein conjugates for antibody production against small molecules","volume":"15","author":"Singh","year":"2004","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0140","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.foodchem.2003.11.006","article-title":"Characterization and safety evaluation of the traditional Greek fruit distillate \u201cMouro\u201d by flavour compounds and mineral analysis","volume":"86","author":"Soufleros","year":"2004","journal-title":"Food Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0145","author":"United State Environmental Protection Agency"},{"key":"10.1016\/j.jim.2012.11.001_bb0170","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1007\/s00217-004-0875-8","article-title":"Study of the mineral profile of Catalonian \u201cbrut\u201d cava using atomic spectrometric methods","volume":"218","author":"Jos","year":"2004","journal-title":"Eur. Food Res. Technol."},{"key":"10.1016\/j.jim.2012.11.001_bb0150","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1021\/ac00192a014","article-title":"Determination of iron, cobalt, copper, rubidium, molybdenum, and cesium in human serum by inductively coupled mass spectrometry","volume":"61","author":"Vanhoe","year":"1989","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0155","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S1872-2040(10)60417-3","article-title":"An indirect enzyme-linked immunosorbent assay for determination of new herbicide H-9201 in water","volume":"39","author":"Zhang","year":"2011","journal-title":"Chin. J. Anal. Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0160","doi-asserted-by":"crossref","first-page":"4993","DOI":"10.1021\/jf070787d","article-title":"Characterization of monoclonal antibodies for lead\u2013chelate complexes: applications in antibody-based assays","volume":"55","author":"Zhu","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jim.2012.11.001_bb0165","doi-asserted-by":"crossref","first-page":"7648","DOI":"10.1021\/jf071025l","article-title":"Preparation of specific monoclonal antibodies against heavy metals: Mabs that recognize chelated cadmium ions","volume":"55","author":"Zhu","year":"2007","journal-title":"J. Agric. Food Chem."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175912003328?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175912003328?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,21]],"date-time":"2018-10-21T15:20:40Z","timestamp":1540135240000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175912003328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":33,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["S0022175912003328"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2012.11.001","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2013,1]]}}}