{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:33:09Z","timestamp":1789389189520,"version":"build-2803163510"},"reference-count":22,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","award":["APQ-01877-12"],"award-info":[{"award-number":["APQ-01877-12"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","award":["APQ-01004-13"],"award-info":[{"award-number":["APQ-01004-13"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","award":["INV-00106-13"],"award-info":[{"award-number":["INV-00106-13"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006182","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Esp\u00edrito Santo","doi-asserted-by":"publisher","award":["50820249\/2010"],"award-info":[{"award-number":["50820249\/2010"]}],"id":[{"id":"10.13039\/501100006182","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.jim.2017.08.005","type":"journal-article","created":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T20:19:14Z","timestamp":1503087554000},"page":"37-47","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening"],"prefix":"10.1016","volume":"451","author":[{"given":"Aline","family":"de Castro Zacche-Tonini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana Schmidt Fran\u00e7a","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura N\u00e9spoli Nassar Pansini","family":"de Jesus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geisa Baptista","family":"Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana Grazziela Alves","family":"Coelho-dos-Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha Ribeiro","family":"B\u00e9la","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson Silva","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Aguiar Vasconcelos","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gl\u00e1ucia Manzan Queiroz","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Vitor","family":"Vasconcelos-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0912-3782","authenticated-orcid":false,"given":"Jos\u00e9 N\u00e9lio","family":"Janu\u00e1rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a","family":"Teixeira-Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Wagner Almeida","family":"Vitor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elo\u00edsa Am\u00e1lia Vieira","family":"Ferro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9010-7228","authenticated-orcid":false,"given":"Jos\u00e9 Roberto","family":"Mineo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olindo Assis","family":"Martins-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elenice Moreira","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2017.08.005_bb0005","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1007\/s13149-013-0287-8","article-title":"D\u00e9pistage de la toxoplasmose materno-foetale: \u00e9tude des cas suivis \u00e0 l'Institut Pasteur de Tunis (2007\u20132010)","volume":"106","author":"Ben Abdallah","year":"2013","journal-title":"Bull. Soc. Pathol. Exot."},{"issue":"2","key":"10.1016\/j.jim.2017.08.005_bb0010","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1590\/S0074-02762009000200038","article-title":"Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis","volume":"104","author":"Bessieres","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"2","key":"10.1016\/j.jim.2017.08.005_bb0015","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1590\/SO100-720320150005115","article-title":"Preval\u00eancia de toxoplasmose em gestantes atendidas em dois centros de refer\u00eancia em uma cidade do Nordeste, Brasil","volume":"37","author":"C\u00e2mara","year":"2015","journal-title":"Rev. Bras. Ginecol. Obstet."},{"issue":"2","key":"10.1016\/j.jim.2017.08.005_bb0020","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1097\/INF.0b013e3181586052","article-title":"Early and accurate diagnosis of congenital toxoplasmosis","volume":"27","author":"Ciardelli","year":"2008","journal-title":"Pediatr. Infect. Dis. J."},{"issue":"1\u20132","key":"10.1016\/j.jim.2017.08.005_bb0025","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jviromet.2009.05.007","article-title":"Performance of IgG and IgG1 anti-HTLV-1 reactivity by an indirect immunofluorescence flow cytometric assay for the identification of persons infected with HTLV-1, asymptomatic carriers and patients with myelopathy","volume":"160","author":"Coelho-dos-Reis","year":"2009","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jim.2017.08.005_bb0030","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jim.2017.02.006","article-title":"Flow cytometric-based protocols for assessing anti-MT-2 IgG1 reactivity: high-dimensional data handling to define predictors for clinical follow-up of Human T-cell Leukemia virus type-1 infection","volume":"444","author":"Coelho-Dos-Reis","year":"2017","journal-title":"J. Immunol. Methods"},{"issue":"4","key":"10.1016\/j.jim.2017.08.005_bb0035","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1086\/590149","article-title":"Management of Toxoplasma gondii infection during pregnancy","volume":"47","author":"Goldstein","year":"2008","journal-title":"Clin. Infect. Dis."},{"issue":"11","key":"10.1016\/j.jim.2017.08.005_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0141700","article-title":"Prevalence of toxoplasmosis in pregnant women and vertical transmission of Toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil, from 2012 to 2014","volume":"10","author":"Gontijo da Silva","year":"2015","journal-title":"PLoS One"},{"issue":"11","key":"10.1016\/j.jim.2017.08.005_bb0045","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1056\/NEJM199409153311102","article-title":"Prenatal diagnosis of congenital toxoplasmosis with a polymerase-chain-reaction test on amniotic fluid","volume":"331","author":"Hohlfeld","year":"1994","journal-title":"N. Engl. J. Med."},{"issue":"4","key":"10.1016\/j.jim.2017.08.005_bb0050","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3347\/kjp.2013.51.4.485","article-title":"IgG western blot for confirmatory diagnosis of equivocal cases of toxoplasmosis by EIA-IgG and fluorescent antibody test","volume":"51","author":"Khammari","year":"2013","journal-title":"Korean J. Parasitol."},{"issue":"3","key":"10.1016\/j.jim.2017.08.005_bb0055","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.amjmed.2004.08.023","article-title":"Toxoplasmosis in pregnancy","volume":"118","author":"Kravetz","year":"2005","journal-title":"Am. J. Med."},{"issue":"6","key":"10.1016\/j.jim.2017.08.005_bb0060","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1128\/JCM.34.3.579-583.1996","volume":"34","author":"Pinon","year":"1996","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.jim.2017.08.005_bb0065","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.1128\/JCM.39.6.2267-2271.2001","article-title":"Strategy for diagnosis of congenital toxoplasmosis: evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and A antibodies","volume":"39","author":"Pinon","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jim.2017.08.005_bb0070","series-title":"Infectious Diseases of the Fetus and Newborn Infant","first-page":"89","article-title":"Toxoplasmosis","author":"Remington","year":"1990"},{"issue":"3","key":"10.1016\/j.jim.2017.08.005_bb0075","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1007\/s10096-003-0906-6","article-title":"Evaluation of a commercial IgG\/IgM Western blot assay for early postnatal diagnosis of congenital toxoplasmosis","volume":"22","author":"Rilling","year":"2003","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"9","key":"10.1016\/j.jim.2017.08.005_bb0080","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1128\/JCM.37.9.2893-2898.1999","article-title":"Value of prenatal diagnosis and early postnatal diagnosis of congenital toxoplasmosis: retrospective study of 110 cases","volume":"37","author":"Robert-Gangneux","year":"1999","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.jim.2017.08.005_bb0085","first-page":"296","article-title":"Prenatal diagnosis using polymerase chain reaction on amniotic fluid for congenital toxoplasmosis","volume":"97","author":"Romand","year":"2001","journal-title":"Obstet. Gynecol."},{"issue":"6","key":"10.1016\/j.jim.2017.08.005_bb0090","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/j.1469-0691.2006.01444.x","article-title":"Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis","volume":"12","author":"Sensini","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"issue":"3","key":"10.1016\/j.jim.2017.08.005_bb0095","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1590\/S0074-02762012000300008","article-title":"Early diagnosis of congenital toxoplasmosis in newborn infants using IgG subclasses against two Toxoplasma gondii recombinant proteins","volume":"107","author":"Silva","year":"2012","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"1\u20132","key":"10.1016\/j.jim.2017.08.005_bb0100","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jim.2012.02.001","article-title":"Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis","volume":"378","author":"Silva-dos-Santos","year":"2012","journal-title":"J. Immunol. Methods"},{"issue":"4857","key":"10.1016\/j.jim.2017.08.005_bb0105","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.3287615","article-title":"Measuring the accuracy of diagnostic systems","volume":"240","author":"Swets","year":"1988","journal-title":"Science"},{"issue":"11","key":"10.1016\/j.jim.2017.08.005_bb0110","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1016\/j.ophtha.2009.04.042","article-title":"Congenital toxoplasmosis in southeastern Brazil: results of early ophthalmologic examination of a large cohort of neonates","volume":"116","author":"Vasconcelos-Santos","year":"2009","journal-title":"Ophthalmology"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217591730282X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217591730282X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T13:02:03Z","timestamp":1659358923000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002217591730282X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":22,"alternative-id":["S002217591730282X"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2017.08.005","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2017.08.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}