{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:06:22Z","timestamp":1769904382377,"version":"3.49.0"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.jim.2019.06.010","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T11:04:09Z","timestamp":1560164649000},"page":"16-24","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Comparative analysis of the germinal center response by flow cytometry and immunohistology"],"prefix":"10.1016","volume":"472","author":[{"given":"Gustaf","family":"Lindgren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Ols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Lor\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2019.06.010_bb0005","doi-asserted-by":"crossref","first-page":"538","DOI":"10.4049\/jimmunol.1701312","article-title":"Quantitative multiplexed imaging analysis reveals a strong association between immunogen-specific B cell responses and tonsillar germinal center immune dynamics in children after influenza vaccination","volume":"200","author":"Amodio","year":"2018","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0010","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.immuni.2011.03.023","article-title":"ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6","volume":"34","author":"Choi","year":"2011","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0015","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.immuni.2011.03.023","article-title":"Bcl6 dependent T follicular helper cell differentiation diverges from effector cell differentiation during priming and depends on the gene Icos","volume":"34","author":"Choi","year":"2011","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0020","series-title":"Tertiary Lymphoid Structures: Methods and Protocols","first-page":"205","article-title":"Identification of tertiary lymphoid structure-associated follicular helper T cells in human tumors and tissues","author":"Couillault","year":"2018"},{"key":"10.1016\/j.jim.2019.06.010_bb0025","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.immuni.2016.12.014","article-title":"Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses","volume":"46","author":"Das","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0030","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1080\/08916934.2017.1280671","article-title":"Spontaneous germinal centers and autoimmunity","volume":"50","author":"Domeier","year":"2017","journal-title":"Autoimmunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0035","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.coviro.2015.04.002","article-title":"Strategies to guide the antibody affinity maturation process","volume":"11","author":"Doria-Rose","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.jim.2019.06.010_bb0040","article-title":"Vaccine priming is restricted to draining lymph nodes and controlled by adjuvant-mediated antigen uptake","volume":"9","author":"Frank Liang","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.jim.2019.06.010_bb0045","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.immuni.2012.07.011","article-title":"Histo-cytometry: a method for highly multiplex quantitative tissue imaging analysis applied to dendritic cell subset microanatomy in lymph nodes","volume":"37","author":"Gerner","year":"2012","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0050","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1016\/j.ajpath.2012.01.012","article-title":"Promotion of a functional B cell germinal center response after Leishmania species co-infection is associated with lesion resolution","volume":"180","author":"Gibson-Corley","year":"2012","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jim.2019.06.010_bb0055","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1073\/pnas.1520112113","article-title":"CXCL13 is a plasma biomarker of germinal center activity","volume":"113","author":"Havenar-Daughton","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jim.2019.06.010_bb0060","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1016\/j.celrep.2016.10.085","article-title":"Direct probing of germinal center responses reveals immunological features and bottlenecks for neutralizing antibody responses to HIV Env trimer","volume":"17","author":"Havenar-Daughton","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.jim.2019.06.010_bb0065","series-title":"Germinal Centers: Methods and Protocols","first-page":"105","article-title":"Follicular dendritic cell isolation and loading of immune complexes","author":"Heesters","year":"2017"},{"key":"10.1016\/j.jim.2019.06.010_bb0070","doi-asserted-by":"crossref","first-page":"797","DOI":"10.4049\/jimmunol.1400749","article-title":"Early lymphoid responses and germinal center formation correlate with lower viral load set points and better prognosis of simian immunodeficiency virus infection","volume":"193","author":"Hong","year":"2014","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0075","series-title":"Germinal Centers: Methods and Protocols","first-page":"51","article-title":"Microanatomical labeling of germinal center structures for flow cytometry using Photoactivation","author":"Jacobsen","year":"2017"},{"key":"10.1016\/j.jim.2019.06.010_bb0080","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/ajt.12526","article-title":"Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection","volume":"14","author":"Kim","year":"2014","journal-title":"Am. J. Transplant. Off. J. Am. Soc. Transplant. Am. Soc. Transplant Surg."},{"key":"10.1016\/j.jim.2019.06.010_bb0085","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrc1589","article-title":"Mechanisms of B-cell lymphoma pathogenesis","volume":"5","author":"Kuppers","year":"2005","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.jim.2019.06.010_bb0090","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aal2094","article-title":"Vaccine priming is restricted to draining lymph nodes and controlled by adjuvant-mediated antigen uptake","volume":"9","author":"Liang","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.jim.2019.06.010_bb0095","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.3389\/fimmu.2017.01539","article-title":"Induction of robust B cell responses after influenza mRNA vaccination is accompanied by circulating hemagglutinin-specific ICOS+ PD-1+ CXCR3+ T follicular helper cells","volume":"8","author":"Lindgren","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0100","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.4049\/jimmunol.1402604","article-title":"Oil-in-water emulsion MF59 increases germinal center B cell differentiation and persistence in response to vaccination","volume":"195","author":"Lofano","year":"2015","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0105","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1128\/CVI.00282-09","article-title":"Toll-like receptor 3 ligand and retinoic acid enhance germinal center formation and increase the tetanus toxoid vaccine response","volume":"16","author":"Ma","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"issue":"804\u2013817","key":"10.1016\/j.jim.2019.06.010_bb0110","article-title":"Particulate Array of well-ordered HIV clade C Env trimers elicits neutralizing antibodies that display a unique V2 cap approach","volume":"46","author":"Martinez-Murillo","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0115","doi-asserted-by":"crossref","first-page":"4836","DOI":"10.4049\/jimmunol.1402071","article-title":"MF59 mediates its B cell Adjuvanticity by promoting T follicular helper cells and thus germinal center responses in adult and early life","volume":"194","author":"Mastelic Gavillet","year":"2015","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0120","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.immuni.2016.09.001","article-title":"Germinal center B cell dynamics","volume":"45","author":"Mesin","year":"2016","journal-title":"Immunity"},{"key":"10.1016\/j.jim.2019.06.010_bb0125","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1172\/JCI63039","article-title":"CD4 T follicular helper cell dynamics during SIV infection","volume":"122","author":"Petrovas","year":"2012","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jim.2019.06.010_bb0130","doi-asserted-by":"crossref","unstructured":"Patrick Schaerli, K. W., Alois B. Lang,\u2021 Martin Lipp,\u00a7 Pius Loetscher, and Bernhard Moser. 2000. CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function. J. Exp. Med. 192: 1553\u20131562.","DOI":"10.1084\/jem.192.11.1553"},{"key":"10.1016\/j.jim.2019.06.010_bb0135","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1084\/jem.20102477","article-title":"A dynamic T cell\u2013limited checkpoint regulates affinity-dependent B cell entry into the germinal center","volume":"208","author":"Schwickert","year":"2011","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jim.2019.06.010_bb0140","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.immuni.2018.06.012","article-title":"PD-1 controls follicular T helper cell positioning and function","volume":"49","author":"Shi","year":"2018","journal-title":"Immunity."},{"key":"10.1016\/j.jim.2019.06.010_bb0145","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.13722","article-title":"The cell proliferation antigen Ki-67 organises heterochromatin","volume":"5","author":"Sobecki","year":"2016","journal-title":"Elife"},{"key":"10.1016\/j.jim.2019.06.010_bb0150","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.4049\/jimmunol.1502137","article-title":"Vaccine induction of lymph node-resident simian immunodeficiency virus Env-specific T follicular helper cells in rhesus macaques","volume":"196","author":"Vargas-Inchaustegui","year":"2016","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0155","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1146\/annurev-immunol-020711-075032","article-title":"Germinal centers","volume":"30","author":"Victora","year":"2012","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.jim.2019.06.010_bb0160","doi-asserted-by":"crossref","first-page":"5475","DOI":"10.1016\/j.vaccine.2014.07.081","article-title":"Lymphatic-targeted cationic liposomes: a robust vaccine adjuvant for promoting long-term immunological memory","volume":"32","author":"Wang","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2019.06.010_bb0165","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1080\/01926230600867727","article-title":"Normal structure, function, and histology of lymph nodes","volume":"34","author":"Willard-Mack","year":"2006","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.jim.2019.06.010_bb0170","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1084\/jem.20180230","article-title":"Activated CARD11 accelerates germinal center kinetics, promoting mTORC1 and terminal differentiation","volume":"215","author":"Wray-Dutra","year":"2018","journal-title":"J. Exp. Med."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919300821?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919300821?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:32:06Z","timestamp":1758299526000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175919300821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":34,"alternative-id":["S0022175919300821"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2019.06.010","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative analysis of the germinal center response by flow cytometry and immunohistology","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2019.06.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}