{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:34:25Z","timestamp":1777476865674,"version":"3.51.4"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.jim.2019.112634","type":"journal-article","created":{"date-parts":[[2019,7,27]],"date-time":"2019-07-27T10:46:39Z","timestamp":1564224399000},"page":"112634","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Inhibition ELISA as a putative tool for the identification and quantification of meningococcal A and X polysaccharides at various stages of vaccine development"],"prefix":"10.1016","volume":"473","author":[{"given":"Nitya","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarmad","family":"Hanif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dilip","family":"Upadhyay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj Kumar","family":"Chhikara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2019.112634_bb0005","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1128\/JCM.37.8.2564-2567.1999","article-title":"Inhibition enzyme-linked immunosorbent assay for serotyping of group B streptococcal isolates","volume":"37","author":"Arakere","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jim.2019.112634_bb0010","first-page":"49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"10.1016\/j.jim.2019.112634_bb0015","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1084\/jem.50.4.533","article-title":"Chemo-immunological studies on conjugated carbohydrate-proteins: ii. Immunological specificity of synthetic sugar-protein antigens","volume":"50","author":"Avery","year":"1929","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jim.2019.112634_bb0020","first-page":"190","article-title":"Quantification of polysaccharide Vi in the typhoid vaccine VaxTyViTM by sandwich ELISA using a monoclonal antibody","volume":"1","author":"Aznar","year":"2016","journal-title":"Bionatura"},{"key":"10.1016\/j.jim.2019.112634_bb0025","doi-asserted-by":"crossref","first-page":"6409","DOI":"10.1016\/j.vaccine.2012.08.021","article-title":"Relative stability of meningococcal serogroup A and X polysaccharides","volume":"30","author":"Berti","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2019.112634_bb0030","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1517\/17460441.2011.609554","article-title":"The design of semi-synthetic and synthetic glycoconjugate vaccines","volume":"6","author":"Costantino","year":"2011","journal-title":"Expert Opin. Drug Discovery"},{"key":"10.1016\/j.jim.2019.112634_bb0035","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1056\/NEJM198709173171201","article-title":"Efficacy of Haemophilus influenzae type b polysaccharide-diphtheria toxoid conjugate vaccine in infancy","volume":"317","author":"Eskola","year":"1987","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2019.112634_bb0040","doi-asserted-by":"crossref","first-page":"6468","DOI":"10.1016\/j.vaccine.2009.06.013","article-title":"Preparation of bacterial polysaccharide-protein conjugates: analytical and manufacturing challenges","volume":"27","author":"Frasch","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2019.112634_bb0045","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/S1474-4422(18)30387-9","article-title":"A systematic analysis for the Global Burden of Disease Study 2016","volume":"17","year":"2018","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.jim.2019.112634_bb0050","series-title":"Validation of Analytical Procedures: Text and Methodology Q2(R1)","article-title":"International conference on harmonisation of technical requirements for registration of pharmaceuticals for human use. ICH harmonised tripartite guideline","author":"ICH","year":"2005"},{"key":"10.1016\/j.jim.2019.112634_bb0055","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1128\/CVI.00302-06","article-title":"Use of an inhibition enzyme-linked immunosorbent assay for quantification of capsular polysaccharide or proteins in vaccines","volume":"14","author":"Inzana","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.jim.2019.112634_bb0060","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2012.0147","article-title":"The impact of protein-conjugate polysaccharide vaccines: an endgame for meningitis?","volume":"368","author":"Maiden","year":"2013","journal-title":"Philos. Trans. R. Soc. B"},{"key":"10.1016\/j.jim.2019.112634_bb0065","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.biologicals.2015.06.008","article-title":"Patterns of binding of aluminum-containing adjuvants to Haemophilus influenzae type b and meningococcal group C conjugate vaccines and components","volume":"43","author":"Otto","year":"2015","journal-title":"Biologicals"},{"key":"10.1016\/j.jim.2019.112634_bb0070","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/0140-6736(92)92117-X","article-title":"Rapid disappearance of Haemophilus influenzae type b meningitis after routine childhood immunisation with conjugate vaccines","volume":"340","author":"Peltola","year":"1992","journal-title":"Lancet"},{"key":"10.1016\/j.jim.2019.112634_bb0075","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/nri2494","article-title":"Maintaining protection against invasive bacteria with protein\u2013polysaccharide conjugate vaccines","volume":"9","author":"Pollard","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jim.2019.112634_bb0080","series-title":"Method of Producing Meningococcal Meningitis Vaccine for Neisseria meningitidis Serotypes A C, Y, and W-135","author":"Reddy","year":"2009"},{"key":"10.1016\/j.jim.2019.112634_bb0085","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jim.2014.03.020","article-title":"Development of four sandwich ELISAs for quantitation of capsular polysaccharides from Neisseria meningitidis serogroups A, C, W and Y in multivalent vaccines","volume":"407","author":"Reyes","year":"2014","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2019.112634_bb0090","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.biologicals.2015.06.003","article-title":"Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C","volume":"43","author":"Sharma","year":"2015","journal-title":"Biologicals"},{"key":"10.1016\/j.jim.2019.112634_bb0095","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.vaccine.2018.12.051","article-title":"Evaluation of impact of temperature and pH alterations on the size and antigenicity of meningococcal serogroup A and X polysaccharides and conjugates","volume":"37","author":"Sharma","year":"2019","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2019.112634_bb0100","series-title":"Recommendations for the Production and Control of Meningococcal Group C Conjugate Vaccines. Annex 2","volume":"vol. 2004","author":"WHO","year":"2004"},{"key":"10.1016\/j.jim.2019.112634_bb0105","article-title":"Recommendations to assure the quality, safety and efficacy of group A meningococcal conjugate vaccines","volume":"2","author":"WHO","year":"2011","journal-title":"Annexure"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919300754?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919300754?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:33:54Z","timestamp":1758299634000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175919300754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":21,"alternative-id":["S0022175919300754"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2019.112634","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Inhibition ELISA as a putative tool for the identification and quantification of meningococcal A and X polysaccharides at various stages of vaccine development","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2019.112634","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"112634"}}