{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:30:49Z","timestamp":1774974649136,"version":"3.50.1"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T00:00:00Z","timestamp":1571356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014452","name":"College of Medicine, University of Cincinnati","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014452","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["U01DA039550"],"award-info":[{"award-number":["U01DA039550"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009712","name":"Dravet Syndrome Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009712","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.1016\/j.jim.2019.112676","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T19:18:22Z","timestamp":1571426302000},"page":"112676","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics"],"prefix":"10.1016","volume":"476","author":[{"given":"Terence L.","family":"Kirley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew B.","family":"Norman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna N.","family":"Wetzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2019.112676_bb0005","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1038\/s41598-018-37072-x","article-title":"Isothermal analysis of ThermoFluor data can readily provide quantitative binding affinities","volume":"9","author":"Bai","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jim.2019.112676_bb0010","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/bs.mie.2018.09.015","article-title":"A guide to run affinity screens using differential scanning Fluorimetry and surface Plasmon resonance assays","volume":"610","author":"Bergsdorf","year":"2018","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jim.2019.112676_bb0015","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1007\/s11095-007-9516-9","article-title":"Extrinsic fluorescent dyes as tools for protein characterization","volume":"25","author":"Hawe","year":"2008","journal-title":"Pharm. Res."},{"key":"10.1016\/j.jim.2019.112676_bb0020","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.abb.2012.09.008","article-title":"Differential scanning calorimetry as a tool for protein folding and stability","volume":"531","author":"Johnson","year":"2013","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.jim.2019.112676_bb0025","doi-asserted-by":"crossref","first-page":"458","DOI":"10.4161\/21645515.2014.990856","article-title":"Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its fab fragment","volume":"11","author":"Kirley","year":"2015","journal-title":"Human Vacc. Immunotherapeut."},{"key":"10.1016\/j.jim.2019.112676_bb0030","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.bbrc.2016.10.128","article-title":"Selective disulfide reduction for labeling and enhancement of fab antibody fragments","volume":"480","author":"Kirley","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jim.2019.112676_bb0035","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.bbrc.2016.06.099","article-title":"Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis","volume":"477","author":"Kirley","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jim.2019.112676_bb0040","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.drugalcdep.2014.04.003","article-title":"Vaccine for cocaine dependence: a randomized double-blind placebo-controlled efficacy trial","volume":"140","author":"Kosten","year":"2014","journal-title":"Drug Alcohol Depend."},{"key":"10.1016\/j.jim.2019.112676_bb0045","doi-asserted-by":"crossref","first-page":"422","DOI":"10.4161\/mabs.3.5.16983","article-title":"Neonatal fc receptor and IgG-based therapeutics","volume":"3","author":"Kuo","year":"2011","journal-title":"mAbs"},{"key":"10.1016\/j.jim.2019.112676_bb0050","doi-asserted-by":"crossref","first-page":"10831","DOI":"10.1021\/bi101414z","article-title":"Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes","volume":"49","author":"Layton","year":"2010","journal-title":"Biochemistry"},{"key":"10.1016\/j.jim.2019.112676_bb0055","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1002\/pro.674","article-title":"Quantitation of protein-protein interactions by thermal stability shift analysis","volume":"20","author":"Layton","year":"2011","journal-title":"Protein Sci."},{"key":"10.1016\/j.jim.2019.112676_bb0060","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1124\/dmd.118.083857","article-title":"A recombinant humanized Anticocaine monoclonal antibody alters the urinary clearance of cocaine and its metabolites in rats","volume":"47","author":"Marckel","year":"2019","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.jim.2019.112676_bb0065","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1001\/archgenpsychiatry.2009.128","article-title":"Cocaine vaccine for the treatment of cocaine dependence in methadone-maintained patients: a randomized, double-blind, placebo-controlled efficacy trial","volume":"66","author":"Martell","year":"2009","journal-title":"Arch. Gen. Psychiatry"},{"key":"10.1016\/j.jim.2019.112676_bb0070","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11095-018-2361-1","article-title":"High throughput differential scanning Fluorimetry (DSF) formulation screening with complementary dyes to assess protein unfolding and aggregation in presence of surfactants","volume":"35","author":"McClure","year":"2018","journal-title":"Pharm. Res."},{"key":"10.1016\/j.jim.2019.112676_bb0075","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1002\/jps.23405","article-title":"High-throughput melting-temperature analysis of a monoclonal antibody by differential scanning fluorimetry in the presence of surfactants","volume":"102","author":"Menzen","year":"2013","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.jim.2019.112676_bb0080","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1177\/1087057114557781","article-title":"High-throughput screening of formulations to optimize the thermal stability of a therapeutic monoclonal antibody","volume":"20","author":"Niedziela-Majka","year":"2015","journal-title":"J. Biomol. Screen."},{"key":"10.1016\/j.jim.2019.112676_bb0085","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1124\/dmd.114.057034","article-title":"A recombinant humanized anti-cocaine monoclonal antibody inhibits the distribution of cocaine to the brain in rats","volume":"42","author":"Norman","year":"2014","journal-title":"Drug Metabolism and Disposition: The Biological Fate of Chemicals"},{"key":"10.1016\/j.jim.2019.112676_bb0090","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0022-2836(02)01235-4","article-title":"Three-dimensional structure-activity relationship modeling of cocaine binding to two monoclonal antibodies by comparative molecular field analysis","volume":"325","author":"Paula","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jim.2019.112676_bb0095","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1021\/jm030351z","article-title":"Three-dimensional quantitative structure-activity relationship modeling of cocaine binding by a novel human monoclonal antibody","volume":"47","author":"Paula","year":"2004","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.jim.2019.112676_bb0100","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0040518","article-title":"Probing cocaine-antibody interactions in buffer and human serum","volume":"7","author":"Ramakrishnan","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jim.2019.112676_bb0105","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.intimp.2014.10.025","article-title":"The effects of a humanized recombinant anti-cocaine monoclonal antibody on the disposition of cocaethylene in mice","volume":"23","author":"Wetzel","year":"2014","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.jim.2019.112676_bb0110","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.drugalcdep.2016.09.024","article-title":"The effects of a repeated dose of a recombinant humanized anti-cocaine monoclonal antibody on cocaine self-administration in rats","volume":"168","author":"Wetzel","year":"2016","journal-title":"Drug Alcohol Depend."},{"key":"10.1016\/j.jim.2019.112676_bb0115","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jim.2017.05.012","article-title":"A novel Python program for implementation of quality control in the ELISA","volume":"448","author":"Wetzel","year":"2017","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2019.112676_bb0120","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.bbrc.2017.04.117","article-title":"Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate","volume":"487","author":"Wetzel","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jim.2019.112676_bb0125","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.lfs.2017.07.006","article-title":"A mathematical model of a recombinant humanized anti-cocaine monoclonal antibody\u2019s effects on cocaine pharmacokinetics in mice","volume":"184","author":"Wetzel","year":"2017","journal-title":"Life Sci."},{"key":"10.1016\/j.jim.2019.112676_bb0130","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1177\/1087057116646553","article-title":"Simultaneous high-throughput conformational and colloidal stability screening using a fluorescent molecular rotor dye, 4-(4-(Dimethylamino)styryl)-N-Methylpyridinium iodide (DASPMI)","volume":"21","author":"Wong","year":"2016","journal-title":"J. Biomol. Screen."},{"key":"10.1016\/j.jim.2019.112676_bb0135","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1021\/acs.jpclett.6b02894","article-title":"Extraction of thermodynamic parameters of protein unfolding using parallelized differential scanning fluorimetry","volume":"8","author":"Wright","year":"2017","journal-title":"J. Phys. Chem. Letters"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919303035?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919303035?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T21:26:44Z","timestamp":1762205204000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175919303035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":27,"alternative-id":["S0022175919303035"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2019.112676","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2019.112676","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"112676"}}