{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:27:57Z","timestamp":1773808077818,"version":"3.50.1"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004695","name":"Inyuvesi Yakwazulu-Natali","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004695","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001322","name":"South African Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1016\/j.jim.2019.112724","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T08:37:34Z","timestamp":1576485454000},"page":"112724","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Isolation of Nile crocodile (Crocodylus niloticus) serum immunoglobulin M and Y (IgM and IgY)"],"prefix":"10.1016","volume":"478","author":[{"given":"Faiaz","family":"Shaik Abdool","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa H.T.","family":"Coetzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.P. Dean","family":"Goldring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2019.112724_bb0005","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/pmic.200300465","article-title":"An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum","volume":"3","author":"Ahmed","year":"2003","journal-title":"Proteomics"},{"key":"10.1016\/j.jim.2019.112724_bb0010","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1042\/bj1670301","article-title":"The effect of matrix on the binding of albumin to immobilized Cibacron blue","volume":"167","author":"Angal","year":"1977","journal-title":"Biochem. J."},{"issue":"Suppl. 7","key":"10.1016\/j.jim.2019.112724_bb0015","first-page":"S131","article-title":"Crocodylus siamensis serum and macrophage phagocytic activity","volume":"94","author":"Aree","year":"2011","journal-title":"J. Med. Assoc. Thailand = Chotmaihet thangphaet"},{"key":"10.1016\/j.jim.2019.112724_bb0020","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1002\/pmic.200401065","article-title":"Effective depletion of albumin using a new peptide-based affinity medium","volume":"5","author":"Baussant","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.jim.2019.112724_bb0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0117394","article-title":"Bioprospecting the American alligator (Alligator mississippiensis) host defense peptidome","volume":"10","author":"Bishop","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.jim.2019.112724_bb0030","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0165-2427(83)90056-9","article-title":"Bovine immunoglobulins: an augmented review","volume":"4","author":"Butler","year":"1983","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.jim.2019.112724_bb0035","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1590\/S0100-736X2015001300009","article-title":"Biochemical serum concentrations of adult male caimans (Melanosuchus niger) in free life","volume":"35","author":"Caixeta","year":"2015","journal-title":"Pesqui. Vet. Bras."},{"key":"10.1016\/j.jim.2019.112724_bb0040","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1042\/BCJ20170334","article-title":"As-CATH1-6, novel cathelicidins with potent antimicrobial and immunomodulatory properties from Alligator sinensis, play pivotal roles in host antimicrobial immune responses","volume":"474","author":"Chen","year":"2017","journal-title":"Biochem. J."},{"key":"10.1016\/j.jim.2019.112724_bb0045","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms2317","article-title":"Extensive diversification of IgH subclass-encoding genes and IgM subclass switching in crocodilians","volume":"4","author":"Cheng","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jim.2019.112724_bb0050","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1186\/1475-2875-11-397","article-title":"A Plasmodium falciparum copper-binding membrane protein with copper transport motifs","volume":"11","author":"Choveaux","year":"2012","journal-title":"Malar. J."},{"key":"10.1016\/j.jim.2019.112724_bb0055","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.exppara.2014.11.001","article-title":"Identification and initial characterisation of a Plasmodium falciparum Cox17 copper metallochaperone","volume":"148","author":"Choveaux","year":"2015","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.jim.2019.112724_bb0060","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0022-1759(84)90249-7","article-title":"Improved method for IgG purification from various animal species by ion exchange chromatography","volume":"66","author":"Corthier","year":"1984","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2019.112724_bb0065","doi-asserted-by":"crossref","first-page":"3638","DOI":"10.1002\/elps.201100307","article-title":"Plasma proteomics for biomarker discovery: a study in blue","volume":"32","author":"Di Girolamo","year":"2011","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jim.2019.112724_bb0070","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1042\/bj1150371","article-title":"Goat immunolobulin G. peptide chains and terminal residues","volume":"115","author":"Givol","year":"1969","journal-title":"Biochem. J."},{"key":"10.1016\/j.jim.2019.112724_bb0075","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/bmb.2003.494031030213","article-title":"Isolation of chicken immunoglobulins (IgY) from egg yolk","volume":"31","author":"Goldring","year":"2003","journal-title":"Biochem. Mol. Biol. Educ."},{"key":"10.1016\/j.jim.2019.112724_bb0080","doi-asserted-by":"crossref","first-page":"1254449","DOI":"10.1126\/science.1254449","article-title":"Three crocodilian genomes reveal ancestral patterns of evolution among archosaurs","volume":"346","author":"Green","year":"2014","journal-title":"Science (New York, N.Y.)"},{"key":"10.1016\/j.jim.2019.112724_bb0085","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jchromb.2006.08.017","article-title":"Thiophilic adsorption revisited","volume":"845","author":"Hardouin","year":"2007","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jim.2019.112724_bb0090","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.toxicon.2005.08.004","article-title":"Factors associated with adverse reactions induced by caprylic acid-fractionated whole IgG preparations: comparison between horse, sheep and camel IgGs","volume":"46","author":"Herrera","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.jim.2019.112724_bb0095","doi-asserted-by":"crossref","first-page":"723","DOI":"10.3390\/ph7060723","article-title":"Antimicrobial peptides in reptiles","volume":"7","author":"van Hoek","year":"2014","journal-title":"Pharmaceuticals (Basel)"},{"key":"10.1016\/j.jim.2019.112724_bb0100","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.peptides.2010.01.002","article-title":"Anti-peptide antibodies differentiate between plasmodial lactate dehydrogenases","volume":"31","author":"Hurdayal","year":"2010","journal-title":"Peptides"},{"key":"10.1016\/j.jim.2019.112724_bb0105","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/0022-4731(77)90196-0","article-title":"Study of steroid-protein binding by a novel \"two-tier\" column employing Cibacron Blue F3G-A-Sepharose 4B. I-Sex hormone binding globulin","volume":"8","author":"Iqbal","year":"1977","journal-title":"J. Steroid Biochem."},{"key":"10.1016\/j.jim.2019.112724_bb0110","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s10930-014-9569-7","article-title":"Purification, characterization, and crystallization of Crocodylus siamensis hemoglobin","volume":"33","author":"Jandaruang","year":"2014","journal-title":"Protein J."},{"key":"10.1016\/j.jim.2019.112724_bb0115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0087534","article-title":"Selection and trans-species polymorphism of major histocompatibility complex class II genes in the order Crocodylia","volume":"9","author":"Jaratlerdsiri","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jim.2019.112724_bb0120","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0022-1759(81)90333-1","article-title":"Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG","volume":"46","author":"Jensenius","year":"1981","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2019.112724_bb0125","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1097\/QAD.0000000000001159","article-title":"Extracellular histones identified in crocodile blood inhibit in-vitro HIV-1 infection","volume":"30","author":"Kozlowski","year":"2016","journal-title":"AIDS"},{"key":"10.1016\/j.jim.2019.112724_bb0130","doi-asserted-by":"crossref","first-page":"627","DOI":"10.3109\/08820139.2015.1070268","article-title":"Comparing antibody responses in chickens against Plasmodium falciparum lactate dehydrogenase and Glyceraldehyde-3-phosphate dehydrogenase with Freund\u2019s and Pheroid(R) adjuvants","volume":"44","author":"Krause","year":"2015","journal-title":"Immunol. Investig."},{"key":"10.1016\/j.jim.2019.112724_bb0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0193833","article-title":"Phosphoethanolamine-N-methyltransferase is a potential biomarker for the diagnosis of P. knowlesi and P. falciparum malaria","volume":"13","author":"Krause","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.jim.2019.112724_bb0140","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.exppara.2017.05.007","article-title":"Plasmodium glyceraldehyde-3-phosphate dehydrogenase: A potential malaria diagnostic target","volume":"179","author":"Krause","year":"2017","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.jim.2019.112724_bb0145","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.2174\/0929866521666140819122624","article-title":"Roles of secreted phospholipases A(2) in the mammalian immune system","volume":"21","author":"Krizaj","year":"2014","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.jim.2019.112724_bb0150","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.4102\/jsava.v85i1.1085","article-title":"Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus) experimentally infected with Trichinella zimbabwensis","volume":"85","author":"La Grange","year":"2014","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"10.1016\/j.jim.2019.112724_bb0155","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.jim.2019.112724_bb0160","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1042\/bj1890027","article-title":"Studies on the mechanism of binding of serum albumins to immobilized cibacron blue F3G A","volume":"189","author":"Leatherbarrow","year":"1980","journal-title":"Biochem. J."},{"key":"10.1016\/j.jim.2019.112724_bb0165","doi-asserted-by":"crossref","first-page":"448","DOI":"10.3181\/00379727-124-31761","article-title":"Comparative immunology. Active immunization of young alligators with hemocyanin","volume":"124","author":"Lerch","year":"1967","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"10.1016\/j.jim.2019.112724_bb0170","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.1093\/nar\/10.11.3371","article-title":"Multiple components in restriction enzyme digests of mammalian (insectivore), avian and reptilian genomic DNA hybridize with murine immunoglobulin VH probes","volume":"10","author":"Litman","year":"1982","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jim.2019.112724_bb0175","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/303349a0","article-title":"Complete nucleotide sequence of an immunoglobulin VH gene homologue from Caiman, a phylogenetically ancient reptile","volume":"303","author":"Litman","year":"1983","journal-title":"Nature"},{"key":"10.1016\/j.jim.2019.112724_bb0180","doi-asserted-by":"crossref","first-page":"137","DOI":"10.4102\/jsava.v78i3.305","article-title":"Normal haematology and blood biochemistry of wild Nile crocodiles (Crocodylus niloticus) in the Okavango Delta, Botswana","volume":"78","author":"Lovely","year":"2007","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"10.1016\/j.jim.2019.112724_bb0185","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00251-013-0692-y","article-title":"IgH loci of American alligator and saltwater crocodile shed light on IgA evolution","volume":"65","author":"Magadan-Mompo","year":"2013","journal-title":"Immunogenetics"},{"key":"10.1016\/j.jim.2019.112724_bb0190","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1093\/clinchem\/30.3.475","article-title":"Sodium dodecyl sulfate polyacrylamide gel electrophoresis of serum after protein denaturation in the presence or absence of 2-mercaptoethanol","volume":"30","author":"Marshall","year":"1984","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jim.2019.112724_bb0195","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.cbpa.2006.01.009","article-title":"Characterization of serum complement activity of saltwater (Crocodylus porosus) and freshwater (Crocodylus johnstoni) crocodiles","volume":"143","author":"Merchant","year":"2006","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"10.1016\/j.jim.2019.112724_bb0200","doi-asserted-by":"crossref","first-page":"925012","DOI":"10.1155\/2011\/925012","article-title":"Characterization of serum phospholipase a(2) activity in three diverse species of west african crocodiles","volume":"2011","author":"Merchant","year":"2011","journal-title":"Biochem. Res. Int."},{"key":"10.1016\/j.jim.2019.112724_bb0205","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.cbpc.2005.03.009","article-title":"Identification of alternative pathway serum complement activity in the blood of the American alligator (Alligator mississippiensis)","volume":"141","author":"Merchant","year":"2005","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.jim.2019.112724_bb0210","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.dci.2010.12.011","article-title":"Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts","volume":"35","author":"Pata","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.jim.2019.112724_bb0215","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0022-1759(90)90458-8","article-title":"Purification of human immunoglobulins by sequential precipitation with caprylic acid and ammonium sulphate","volume":"128","author":"Perosa","year":"1990","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2019.112724_bb0220","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3109\/08820138509022667","article-title":"Improvements in the isolation of IgY from the yolks of eggs laid by immunized hens","volume":"14","author":"Polson","year":"1985","journal-title":"Immunol. Investig."},{"key":"10.1016\/j.jim.2019.112724_bb0225","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/0014-5793(85)80928-5","article-title":"Thiophilic adsorption--a new method for protein fractionation","volume":"185","author":"Porath","year":"1985","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jim.2019.112724_bb0230","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1016\/j.jprot.2012.01.004","article-title":"Identification of five reptile egg whites protein using MALDI-TOF mass spectrometry and LC\/MS-MS analysis","volume":"75","author":"Prajanban","year":"2012","journal-title":"J. Proteome"},{"key":"10.1016\/j.jim.2019.112724_bb0235","first-page":"393","article-title":"Isolation and characterisation of crocosin, an antibacterial compound from crocodile (Crocodylus siamensis) plasma","volume":"81","author":"Preecharram","year":"2010","journal-title":"Animal science journal = Nihon chikusan Gakkaiho"},{"key":"10.1016\/j.jim.2019.112724_bb0240","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.chroma.2006.04.012","article-title":"Novel hydrophobic interaction chromatography matrix for specific isolation and simple elution of immunoglobulins (a, G, and M) from porcine serum","volume":"1122","author":"Ramos-Clamont","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jim.2019.112724_bb0245","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1939-165X.2011.00344.x","article-title":"Hematologic and serum biochemical reference values for the wild spectacled Caiman, Caiman crocodilus crocodilus, from the Venezuelan plains","volume":"40","author":"Rossini","year":"2011","journal-title":"Vet. Clin. Pathol."},{"key":"10.1016\/j.jim.2019.112724_bb0250","doi-asserted-by":"crossref","first-page":"959","DOI":"10.7589\/2015-03-064","article-title":"Hematology and serum biochemistry for free-ranging freshwater crocodiles (Crocodylus johnstoni) in Western Australia","volume":"52","author":"Scheelings","year":"2016","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.jim.2019.112724_bb0255","series-title":"Protein Purification\u202f: Principles and Practice","author":"Scopes","year":"1994"},{"key":"10.1016\/j.jim.2019.112724_bb0260","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jim.2019.112724_bb0265","article-title":"A comprehensive analysis of the phylogeny, genomic organization and expression of immunoglobulin light chain genes in Alligator sinensis, an endangered reptile species","volume":"11","author":"Wang","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.jim.2019.112724_bb0270","doi-asserted-by":"crossref","first-page":"e305","DOI":"10.1038\/emm.2017.23","article-title":"From rabbit antibody repertoires to rabbit monoclonal antibodies","volume":"49","author":"Weber","year":"2017","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.jim.2019.112724_bb0275","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1242\/jeb.038315","article-title":"Understanding the vertebrate immune system: insights from the reptilian perspective","volume":"213","author":"Zimmerman","year":"2010","journal-title":"J. Exp. Biol."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919304594?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175919304594?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:40:46Z","timestamp":1758300046000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175919304594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":55,"alternative-id":["S0022175919304594"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2019.112724","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation of Nile crocodile (Crocodylus niloticus) serum immunoglobulin M and Y (IgM and IgY)","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2019.112724","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112724"}}