{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:09:39Z","timestamp":1787836179555,"version":"build-2784847793"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HHSN272201300018I"],"award-info":[{"award-number":["HHSN272201300018I"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001203","name":"GlaxoSmithKline Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001203","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007623","name":"School of Medicine, Emory University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007623","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004628","name":"MedImmune","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008065","name":"Georgia Research Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012423","name":"Children's Healthcare of Atlanta","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012423","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014101","name":"Novavax","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014101","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014588","name":"Sanofi Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014588","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006939","name":"Emory University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006939","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005565","name":"Janssen Biotech","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005565","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.jim.2020.112900","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T04:02:01Z","timestamp":1602820921000},"page":"112900","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay"],"prefix":"10.1016","volume":"488","author":[{"given":"Xuemin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry J.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Rostad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingmei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilin","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mulligan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Rouphael","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muktha S.","family":"Natrajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"McCracken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan J.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2020.112900_bb0005","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/s41579-018-0039-7","article-title":"Zika virus vaccines","volume":"16","author":"Abbink","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jim.2020.112900_bb0010","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.biopha.2017.05.045","article-title":"Recent trends in ZikV research: a step away from cure","volume":"91","author":"Alam","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.jim.2020.112900_bb0015","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1002\/eji.201344179","article-title":"Integrin receptors on tumor cells facilitate NK cell-mediated antibody-dependent cytotoxicity","volume":"44","author":"Anikeeva","year":"2014","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0020","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00405-19","article-title":"Human monoclonal antibodies potently neutralize Zika virus and select for escape mutations on the lateral ridge of the envelope protein","volume":"93","author":"Bailey","year":"2019","journal-title":"J. Virol."},{"key":"10.1016\/j.jim.2020.112900_bb0025","doi-asserted-by":"crossref","first-page":"6392","DOI":"10.4049\/jimmunol.180.9.6392","article-title":"Blocking NK cell inhibitory self-recognition promotes antibody-dependent cellular cytotoxicity in a model of anti-lymphoma therapy","volume":"180","author":"Binyamin","year":"2008","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0030","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.3389\/fimmu.2018.02551","article-title":"Mechanisms of NK cell activation and clinical activity of the therapeutic SLAMF7 antibody, Elotuzumab in multiple myeloma","volume":"9","author":"Campbell","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0035","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.jviromet.2014.04.019","article-title":"An optimized expression vector for improving the yield of dengue virus-like particles from transfected insect cells","volume":"205","author":"Charoensri","year":"2014","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jim.2020.112900_bb0040","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.3389\/fimmu.2018.02322","article-title":"The early antibody-dependent cell-mediated cytotoxicity response is associated with lower viral set point in individuals with primary HIV infection","volume":"9","author":"Chen","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0045","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.chom.2016.04.013","article-title":"Structures of the Zika virus envelope protein and its complex with a Flavivirus broadly protective antibody","volume":"19","author":"Dai","year":"2016","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.jim.2020.112900_bb0050","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1056\/NEJMc1608612","article-title":"Zika virus infection and associated neurologic disorders in Brazil","volume":"376","author":"de Oliveira","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2020.112900_bb0055","doi-asserted-by":"crossref","DOI":"10.3390\/v10090480","article-title":"Improved immune responses against Zika virus after sequential dengue and Zika virus infection in humans","volume":"10","author":"Delgado","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.jim.2020.112900_bb0060","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1097\/QAD.0000000000002011","article-title":"Antibody-dependent cellular cytotoxicity in HIV infection","volume":"32","author":"Forthal","year":"2018","journal-title":"AIDS"},{"key":"10.1016\/j.jim.2020.112900_bb0065","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00430-013-0321-2","article-title":"Combination of immunoglobulins and natural killer cells in the context of CMV and EBV infection","volume":"203","author":"Frenzel","year":"2014","journal-title":"Med. Microbiol. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0070","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1128\/JCM.02469-15","article-title":"Establishment of an algorithm using prM\/E- and NS1-specific IgM antibody-capture enzyme-linked Immunosorbent assays in diagnosis of Japanese encephalitis virus and West Nile virus infections in humans","volume":"54","author":"Galula","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jim.2020.112900_bb0075","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00834-17","article-title":"Development of virus-like-particle vaccine and reporter assay for Zika virus","volume":"91","author":"Garg","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.jim.2020.112900_bb0080","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/S0140-6736(17)33105-7","article-title":"Safety, tolerability, and immunogenicity of two Zika virus DNA vaccine candidates in healthy adults: randomised, open-label, phase 1 clinical trials","volume":"391","author":"Gaudinski","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.jim.2020.112900_bb0085","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1172\/JCI64837","article-title":"Human immunodeficiency-causing mutation defines CD16 in spontaneous NK cell cytotoxicity","volume":"122","author":"Grier","year":"2012","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jim.2020.112900_bb0090","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1056\/NEJMoa1709481","article-title":"Pregnancy outcomes after ZIKV infection in French territories in the Americas","volume":"378","author":"Hoen","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2020.112900_bb0095","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.3389\/fimmu.2018.02494","article-title":"Natural killer cells adapt to Cytomegalovirus along a functionally static phenotypic Spectrum in human immunodeficiency virus infection","volume":"9","author":"Holder","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0100","doi-asserted-by":"crossref","first-page":"e147","DOI":"10.1016\/S1473-3099(17)30422-X","article-title":"Protection of the house against Chagas disease, dengue, leishmaniasis, and lymphatic filariasis: a systematic review","volume":"18","author":"Horstick","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jim.2020.112900_bb0105","first-page":"818","article-title":"Induction of H7N9-cross-reactive antibody-dependent cellular cytotoxicity antibodies by human seasonal influenza a viruses that are directed toward the nucleoprotein","volume":"215","author":"Jegaskanda","year":"2017","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jim.2020.112900_bb0110","doi-asserted-by":"crossref","first-page":"86359","DOI":"10.18632\/oncotarget.13411","article-title":"An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele","volume":"7","author":"Jochems","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.jim.2020.112900_bb0115","series-title":"Zika: The Origin and Spread of a Mosquito-Borne Virus","author":"Kindhauser","year":"2016"},{"key":"10.1016\/j.jim.2020.112900_bb0125","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/cid\/cix732","article-title":"Innate, T-, and B-cell responses in acute human Zika patients","volume":"66","author":"Lai","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jim.2020.112900_bb0130","doi-asserted-by":"crossref","first-page":"933","DOI":"10.3201\/eid2205.160065","article-title":"Phylogeny of Zika virus in Western hemisphere, 2015","volume":"22","author":"Lanciotti","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jim.2020.112900_bb0135","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1038\/s41467-018-05519-4","article-title":"Critical role of CD4(+) T cells and IFNgamma signaling in antibody-mediated resistance to Zika virus infection","volume":"9","author":"Lucas","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jim.2020.112900_bb0140","doi-asserted-by":"crossref","first-page":"481","DOI":"10.3389\/fimmu.2013.00481","article-title":"Combination immune therapies to enhance anti-tumor responses by NK cells","volume":"4","author":"Mentlik James","year":"2013","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0145","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1056\/NEJMra1808246","article-title":"Zika virus infection - after the pandemic","volume":"381","author":"Musso","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2020.112900_bb0150","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.3389\/fmicb.2018.01797","article-title":"Role of Zika virus prM protein in viral pathogenicity and use in vaccine development","volume":"9","author":"Nambala","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jim.2020.112900_bb0155","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES2014.19.9.20720","article-title":"Zika virus infection complicated by Guillain-Barre syndrome--case report, French Polynesia, December 2013","volume":"19","author":"Oehler","year":"2014","journal-title":"Euro Surveill"},{"key":"10.1016\/j.jim.2020.112900_bb0160","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1016\/j.jhep.2017.01.032","article-title":"Hepatitis C virus-induced NK cell activation causes metzincin-mediated CD16 cleavage and impaired antibody-dependent cytotoxicity","volume":"66","author":"Oliviero","year":"2017","journal-title":"J. Hepatol."},{"key":"10.1016\/j.jim.2020.112900_bb0165","series-title":"Guidelines for surveillance of Zika virus disease and its complications","author":"Pan American Health Organization","year":"2016"},{"key":"10.1016\/j.jim.2020.112900_bb0170","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1056\/NEJMoa1605564","article-title":"Guillain\u2013Barr\u00e9 syndrome associated with Zika virus infection in Colombia","volume":"375","author":"Parra","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jim.2020.112900_bb0175","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1590\/0037-8682-0220-2017","article-title":"Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation","volume":"51","author":"Queiroz","year":"2018","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.jim.2020.112900_bb0180","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.coi.2018.04.024","article-title":"Zika virus vaccines: immune response, current status, and future challenges","volume":"53","author":"Richner","year":"2018","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0185","article-title":"Zika virus activates de novo and cross-reactive memory B cell responses in dengue-experienced donors","volume":"2","author":"Rogers","year":"2017","journal-title":"Scie. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0190","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftz026","article-title":"Pichia pastoris-expressed Zika virus envelope domain III on a virus-like particle platform: design, production and immunological evaluation","volume":"77","author":"Shanmugam","year":"2019","journal-title":"Pathog. Dis."},{"key":"10.1016\/j.jim.2020.112900_bb0195","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1080\/10543406.2014.979196","article-title":"Statistical evaluation of several methods for cut-point determination of immunogenicity screening assay","volume":"25","author":"Shen","year":"2015","journal-title":"J. Biopharm. Stat."},{"key":"10.1016\/j.jim.2020.112900_bb0200","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.38970","article-title":"Epitope resurfacing on dengue virus-like particle vaccine preparation to induce broad neutralizing antibody","volume":"7","author":"Shen","year":"2018","journal-title":"Elife"},{"key":"10.1016\/j.jim.2020.112900_bb0205","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1080\/14760584.2018.1516553","article-title":"Effector mechanisms of influenza-specific antibodies: neutralization and beyond","volume":"17","author":"Sicca","year":"2018","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.jim.2020.112900_bb0210","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jim.2018.06.002","article-title":"A novel Ebola virus antibody-dependent cell-mediated cytotoxicity (Ebola ADCC) assay","volume":"460","author":"Singh","year":"2018","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2020.112900_bb0215","doi-asserted-by":"crossref","first-page":"11833","DOI":"10.1074\/jbc.M114.614719","article-title":"Integrins influence the size and dynamics of Signaling microclusters in a Pyk2-dependent manner","volume":"290","author":"Steblyanko","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jim.2020.112900_bb0220","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.aaf8505","article-title":"Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection","volume":"353","author":"Stettler","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.jim.2020.112900_bb0225","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.3389\/fimmu.2018.02552","article-title":"In vitro evidence that combination therapy with CD16-bearing NK-92 cells and FDA-approved Alefacept can selectively target the latent HIV reservoir in CD4+ CD2hi memory T cells","volume":"9","author":"Tomalka","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0230","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.coviro.2016.12.002","article-title":"Antibody-dependent cellular cytotoxicity and influenza virus","volume":"22","author":"Vanderven","year":"2017","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.jim.2020.112900_bb0235","series-title":"July Zika Epidemiology Update","author":"World Health Organization","year":"2019"},{"key":"10.1016\/j.jim.2020.112900_bb0240","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3389\/fimmu.2017.00317","article-title":"Antibody-dependent cell-mediated cytotoxicity epitopes on the Hemagglutinin head region of pandemic H1N1 influenza virus play detrimental roles in H1N1-infected mice","volume":"8","author":"Ye","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2020.112900_bb0245","doi-asserted-by":"crossref","first-page":"2","DOI":"10.20411\/pai.v3i1.228","article-title":"ADCC-mediated CD56(DIM) NK cell responses are associated with early HBsAg clearance in acute HBV infection","volume":"3","author":"Yu","year":"2018","journal-title":"Pathog. Immun."},{"key":"10.1016\/j.jim.2020.112900_bb0250","doi-asserted-by":"crossref","first-page":"2604","DOI":"10.1093\/emboj\/cdg270","article-title":"Structures of immature flavivirus particles","volume":"22","author":"Zhang","year":"2003","journal-title":"EMBO J."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175920301940?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175920301940?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T21:26:54Z","timestamp":1762205214000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175920301940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":49,"alternative-id":["S0022175920301940"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2020.112900","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2020.112900","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112900"}}