{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:02:12Z","timestamp":1779328932259,"version":"3.51.4"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.jim.2020.112910","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T22:47:15Z","timestamp":1604702835000},"page":"112910","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Positive tissue transglutaminase antibodies with negative endomysial antibodies: Unresolved issues in diagnosing celiac disease"],"prefix":"10.1016","volume":"489","author":[{"given":"Maria","family":"Infantino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Merone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariangela","family":"Manfredi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Grossi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Landini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Alessio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Previtali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Trevisan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brunetta","family":"Porcelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Fabris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donatella","family":"Macchia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danilo","family":"Villalta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Cinquanta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"D'Antoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulio","family":"Iannello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Soda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Bizzaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2020.112910_bb0005","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/cei.13394","article-title":"Antibodies against neo-epitope of microbial and human transglutaminase complexes as biomarkers of childhood celiac disease","volume":"199","author":"Agardh","year":"2020","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.jim.2020.112910_bb0010","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.cca.2013.03.024","article-title":"Chemiluminescence and ELISA-based serum assays for diagnosing and monitoring celiac disease in children: a comparative study","volume":"421","author":"Aita","year":"2013","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.jim.2020.112910_bb0015","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1006\/jaut.1999.0303","article-title":"One third of HLA DQ2 homozygous patients with type 1 diabetes express celiac disease-associated transglutaminase autoantibodies","volume":"13","author":"Bao","year":"1999","journal-title":"J. Autoimmun."},{"key":"10.1016\/j.jim.2020.112910_bb0020","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1023\/B:DDAS.0000007875.72256.e8","article-title":"IgA and IgG tissue transglutaminase antibody prevalence and clinical significance in connective tissue diseases, inflammatory bowel disease, and primary biliary cirrhosis","volume":"48","author":"Bizzaro","year":"2003","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.jim.2020.112910_bb0025","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s12016-010-8223-1","article-title":"Cutting-edge issues in celiac disease and in gluten intolerance","volume":"42","author":"Bizzaro","year":"2012","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"10.1016\/j.jim.2020.112910_bb0030","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1136\/gut.49.4.506","article-title":"Guinea pig transglutaminase immunolinked assay does not predict celiac disease in patients with chronic liver disease","volume":"49","author":"Carroccio","year":"2001","journal-title":"Gut"},{"key":"10.1016\/j.jim.2020.112910_bb0035","first-page":"31","article-title":"Antitissue transglutaminase antibodies outside celiac disease","volume":"34","author":"Clemente","year":"2002","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"10.1016\/j.jim.2020.112910_bb0040","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1136\/gut.35.9.1215","article-title":"Celiac disease-associated disorders and survival","volume":"35","author":"Collin","year":"1994","journal-title":"Gut"},{"key":"10.1016\/j.jim.2020.112910_bb0045","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1136\/gut.35.6.771","article-title":"Serological screening for Celiac disease: choosing the optimal procedure according to various prevalence values","volume":"35","author":"Corrao","year":"1994","journal-title":"Gut"},{"key":"10.1016\/j.jim.2020.112910_bb0050","doi-asserted-by":"crossref","first-page":"100045","DOI":"10.1016\/j.jtauto.2020.100045","article-title":"Testing for IgA anti-tissue transglutaminase in routine clinical practice: requesting behaviour in relation to prevalence of positive results","volume":"3","author":"Damoiseaux","year":"2020","journal-title":"J Transl Autoimmun."},{"key":"10.1016\/j.jim.2020.112910_bb0055","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jim.2016.01.002","article-title":"Fully-automated, chemiluminescence IgA and IgG anti-tissue transglutaminase (tTG) antibodies serum assays for the screening of celiac disease","volume":"429","author":"Daves","year":"2016","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2020.112910_bb0060","doi-asserted-by":"crossref","first-page":"102567","DOI":"10.1016\/j.ebiom.2019.11.028","article-title":"Diagnostic accuracy and applicability of intestinal auto-antibodies in the wide clinical spectrum of Celiac disease","volume":"51","author":"De Leo","year":"2020","journal-title":"EBioMedicine"},{"key":"10.1016\/j.jim.2020.112910_bb0065","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1038\/nm0797-797","article-title":"Identification of tissue transglutaminase as the autoantigen of celiac disease","volume":"3","author":"Dieterich","year":"1997","journal-title":"Nat. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0070","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1111\/j.1365-2796.2011.02380.x","article-title":"What is the use of biopsy and antibodies in Celiac disease diagnosis?","volume":"269","author":"Evans","year":"2011","journal-title":"J. Intern. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0075","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1001\/archinte.163.3.286","article-title":"Prevalence of celiac disease in at risk and not-at-risk groups in the United States: a large multicenter study","volume":"163","author":"Fasano","year":"2003","journal-title":"Arch. Intern. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0080","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/MPG.0b013e31821a23d0","article-title":"European Society for pediatric gastroenterology, hepatology, and nutrition guidelines for diagnosing Celiac disease 2019","volume":"54","author":"Husby","year":"2019","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"10.1016\/j.jim.2020.112910_bb0085","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1023\/A:1026612121041","article-title":"Celiac disease and autoimmune endocrinologic disorders","volume":"44","author":"Kaukinen","year":"1999","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.jim.2020.112910_bb0090","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1515\/CCLM.2010.241","article-title":"Celiac disease a diagnostic and therapeutic challenge","volume":"48","author":"Kaukinen","year":"2010","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0095","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1111\/j.1572-0241.2000.02086.x","article-title":"Detection of autoantibodies against tissue transglutaminase inpatients with celiac disease and dermatitis herpetiformis","volume":"95","author":"Koop","year":"2000","journal-title":"Am. J. Gastroenterol."},{"key":"10.1016\/j.jim.2020.112910_bb0100","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1053\/gast.2001.22161","article-title":"Limitations of anti-Guinea pig liver transglutaminase IgA in screening of celiac disease","volume":"120","author":"Leon","year":"2001","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jim.2020.112910_bb0105","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3109\/08830185.2011.595854","article-title":"An update on the diagnostics of celiac disease","volume":"30","author":"Lindfors","year":"2011","journal-title":"Int. Rev. Immunol."},{"key":"10.1016\/j.jim.2020.112910_bb0110","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1097\/00042737-199910000-00019","article-title":"The histopathology of Celiac disease: time for a standardised report scheme for pathologist","volume":"11","author":"Oberhuber","year":"1999","journal-title":"Eur. J. Gastroenterol. Hepatol"},{"key":"10.1016\/j.jim.2020.112910_bb0115","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1097\/00042737-200211000-00009","article-title":"Production of recombinant human tissue transglutaminase using the baculovirus expression system, and its application for serological diagnosis of Celiac disease","volume":"14","author":"Osman","year":"2002","journal-title":"Eur. J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.jim.2020.112910_bb0120","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s13631-015-0086-2","article-title":"Guidelines for laboratory and histological diagnosis of celiac disease revision 2015","volume":"11","author":"Porcelli","year":"2015","journal-title":"Riv. Ital. Med. Lab"},{"key":"10.1016\/j.jim.2020.112910_bb0125","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1097\/MPG.0000000000001773","article-title":"Celiac disease diagnosis without biopsy: is a 10x ULN anti-transglutaminase result suitable for a chemiluminescence method?","volume":"66","author":"Previtali","year":"2018","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"10.1016\/j.jim.2020.112910_bb0130","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/pat.32.3.181.185","article-title":"The measurement of IgA and IgG transglutaminase antibodies in celiac disease: a comparison with current diagnostic methods","volume":"32","author":"Reeves","year":"2000","journal-title":"Pathology"},{"key":"10.1016\/j.jim.2020.112910_bb0135","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1515\/CCLM.2011.164","article-title":"Use of a fully automated immunoassay for celiac disease screening in a pediatric population","volume":"49","author":"Ress","year":"2011","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0140","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/j.1749-6632.1998.tb11116.x","article-title":"Identification of the autoantigen of celiac disease","volume":"859","author":"Schuppan","year":"1998","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.jim.2020.112910_bb0145","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1006\/prep.2001.1587","article-title":"Expression in Escherichia coli and purification of hexahistidine-tagged human tissue transglutaminase","volume":"24","author":"Shi","year":"2002","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jim.2020.112910_bb0150","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1515\/cclm-2015-0348","article-title":"Immunoassays for the detection of IgA antibodies to tissue transglutaminase: significance of multiples of the upper limit of normal and inter-assay correlations","volume":"54","author":"Suh-Lailam","year":"2016","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.jim.2020.112910_bb0155","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1136\/jcp.56.5.389","article-title":"The role of anti-tissue transglutaminase assay for the diagnosis and monitoring of Celiac disease: a French-Italian multicentre study","volume":"56","author":"Tonutti","year":"2003","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.jim.2020.112910_bb0160","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S0022-3476(99)70410-5","article-title":"IgA antibodies to tissue transglutaminase: an effective diagnostic test for celiac disease","volume":"134","author":"Troncone","year":"1999","journal-title":"J. Pediatr."},{"key":"10.1016\/j.jim.2020.112910_bb0165","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1016\/j.cgh.2018.03.028","article-title":"Comparison of diagnostic performance of the IgA anti-tTG test vs IgA anti-native Gliadin antibodies test in detection of celiac disease in the general population","volume":"16","author":"Verma","year":"2018","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.jim.2020.112910_bb0170","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/cmi.2010.64","article-title":"Celiac disease: diagnostic criteria in progress","volume":"8","author":"Volta","year":"2011","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.jim.2020.112910_bb0175","first-page":"9","article-title":"Positive tissue transglutaminase antibodies with negative endomysial antibodies: low rate of celiac disease","volume":"6","author":"Weiss","year":"2004","journal-title":"Isr. Med. Assoc. J."},{"key":"10.1016\/j.jim.2020.112910_bb0180","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1001\/jama.2010.549","article-title":"Diagnostic testing for celiac disease among patientswith abdominal symptoms: a systematic review","volume":"303","author":"van der Windt","year":"2010","journal-title":"JAMA"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175920302040?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175920302040?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:37:29Z","timestamp":1758299849000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175920302040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":36,"alternative-id":["S0022175920302040"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2020.112910","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Positive tissue transglutaminase antibodies with negative endomysial antibodies: Unresolved issues in diagnosing celiac disease","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2020.112910","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112910"}}