{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T16:42:55Z","timestamp":1767890575148,"version":"3.49.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2017XQC-2"],"award-info":[{"award-number":["2017XQC-2"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2018ZX09J18105-003"],"award-info":[{"award-number":["2018ZX09J18105-003"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2023,2]]},"DOI":"10.1016\/j.jim.2023.113427","type":"journal-article","created":{"date-parts":[[2023,1,15]],"date-time":"2023-01-15T09:26:15Z","timestamp":1673774775000},"page":"113427","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin"],"prefix":"10.1016","volume":"513","author":[{"given":"Yi","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiazhen","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongqi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weicai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghua","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huipeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2023.113427_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxcx.2019.100006","article-title":"An oligoclonal combination of human monoclonal antibodies able to neutralize tetanus toxin in vivo","volume":"2","author":"Aliprandini","year":"2019","journal-title":"Toxicon X"},{"key":"10.1016\/j.jim.2023.113427_bb0010","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1128\/CVI.00763-14","article-title":"A combination of three fully human toxin A- and toxin B-specific monoclonal antibodies protects against challenge with highly virulent epidemic strains of Clostridium difficile in the hamster model","volume":"22","author":"Anosova","year":"2015","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0015","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1126\/science.1258138","article-title":"Nidogens are therapeutic targets for the prevention of tetanus","volume":"346","author":"Bercsenyi","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.jim.2023.113427_bb0020","doi-asserted-by":"crossref","first-page":"6883","DOI":"10.3390\/ijms13066883","article-title":"Fragment C of tetanus toxin: new insights into its neuronal signaling pathway","volume":"13","author":"Calvo","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jim.2023.113427_bb0025","first-page":"175","article-title":"Analysis of cases with adverse reactions caused by tetanus antitoxin","volume":"19","author":"Chen","year":"2019","journal-title":"Pract. Clin. J. Integr. Tradit. Chin. West. Med."},{"key":"10.1016\/j.jim.2023.113427_bb0030","doi-asserted-by":"crossref","first-page":"7179","DOI":"10.1021\/bi800640y","article-title":"Molecular basis for tetanus toxin coreceptor interactions","volume":"47","author":"Chen","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.jim.2023.113427_bb0035","doi-asserted-by":"crossref","first-page":"26569","DOI":"10.1074\/jbc.M109.027391","article-title":"Gangliosides as high affinity receptors for tetanus neurotoxin","volume":"284","author":"Chen","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jim.2023.113427_bb0040","first-page":"603","article-title":"Preparation and identification of monoclonal antibodies against tetanus toxin","volume":"45","author":"Chen","year":"2021","journal-title":"Mil. Med. Sci."},{"key":"10.1016\/j.jim.2023.113427_bb0045","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1017\/S1049023X17000012","article-title":"Tetanus: a potential public health threat in times of disaster","volume":"32","author":"Finkelstein","year":"2017","journal-title":"Prehosp. Disaster Med."},{"key":"10.1016\/j.jim.2023.113427_bb0050","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0264-410X(00)00115-8","article-title":"Inhibition of tetanus toxin fragment C binding to ganglioside GT1b by monoclonal antibodies recognizing different epitopes","volume":"19","author":"Fitzsimmons","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2023.113427_bb0055","doi-asserted-by":"crossref","first-page":"32274","DOI":"10.1074\/jbc.M103285200","article-title":"The crystal structure of tetanus toxin Hc fragment complexed with a synthetic GT1b analogue suggests cross-linking between ganglioside receptors and the toxin","volume":"276","author":"Fotinou","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jim.2023.113427_bb0060","first-page":"117","article-title":"Mortality and causes of death collaborators, 2015. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the global burden of disease study 2013","volume":"385","author":"GBD","year":"2013","journal-title":"Lancet"},{"key":"10.1016\/j.jim.2023.113427_bb0065","first-page":"137","article-title":"Comparison of the use of tetanus human immunoglobulin and horse serum tetanus antitoxin. Shenzhen","volume":"30","author":"Huang","year":"2020","journal-title":"J. Integr. Tradit. Chin. West. Med."},{"key":"10.1016\/j.jim.2023.113427_bb0070","first-page":"31","article-title":"Analysis of morbidity trend of infectious diseases in central and Eastern Europe along the China-Proposed Belt and road initiative","volume":"32","author":"Jiang","year":"2019","journal-title":"Med. Soc."},{"key":"10.1016\/j.jim.2023.113427_bb0080","doi-asserted-by":"crossref","first-page":"466","DOI":"10.4049\/jimmunol.151.1.466","article-title":"Immunotherapy with human monoclonal antibodies. Fragment a specificity of polyclonal and monoclonal antibodies is crucial for full protection against tetanus toxin","volume":"151","author":"Lang","year":"1993","journal-title":"J. Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0085","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1093\/ilar.46.3.258","article-title":"Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources","volume":"46","author":"Lipman","year":"2005","journal-title":"ILAR J."},{"key":"10.1016\/j.jim.2023.113427_bb0090","doi-asserted-by":"crossref","first-page":"13644","DOI":"10.1021\/bi020291j","article-title":"Identification of a binding site for ganglioside on the receptor binding domain of tetanus toxin","volume":"41","author":"Louch","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.jim.2023.113427_bb0095","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.toxicon.2015.06.025","article-title":"Key protection factors against tetanus: anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin \u2013 ganglioside interaction","volume":"103","author":"Luki\u0107","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.jim.2023.113427_bb0100","doi-asserted-by":"crossref","first-page":"3764","DOI":"10.1016\/j.vaccine.2018.05.058","article-title":"Cooperative binding of anti-tetanus toxin monoclonal antibodies: implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication","volume":"36","author":"Lukic","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2023.113427_bb0105","doi-asserted-by":"crossref","first-page":"6449","DOI":"10.1016\/j.vaccine.2012.08.002","article-title":"Identification of a novel linear epitope in tetanus toxin recognized by a protective monoclonal antibody: implications for vaccine design","volume":"30","author":"Luo","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.jim.2023.113427_bb0110","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.15252\/embr.201744198","article-title":"The structure of the tetanus toxin reveals pH-mediated domain dynamics","volume":"18","author":"Masuyer","year":"2017","journal-title":"EMBO Rep."},{"key":"10.1016\/j.jim.2023.113427_bb0115","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1006\/bbrc.2001.6031","article-title":"High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein","volume":"289","author":"Munro","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jim.2023.113427_bb0120","doi-asserted-by":"crossref","first-page":"5047","DOI":"10.1128\/AAC.02830-14","article-title":"Safety and pharmacokinetics of XOMA 3AB, a novel mixture of three monoclonal antibodies against botulinum toxin A","volume":"58","author":"Nayak","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jim.2023.113427_bb0125","doi-asserted-by":"crossref","first-page":"16584","DOI":"10.1038\/s41598-018-35027-w","article-title":"Tetanus toxin C-fragment protects against excitotoxic spinal motoneuron degeneration in vivo","volume":"8","author":"Netzahualcoyotzi","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jim.2023.113427_bb0130","first-page":"1","article-title":"Tetanus surveillance\u2014United States, 1998-2000","volume":"52","author":"Pascual","year":"2003","journal-title":"MMWR Surveill. Summ."},{"key":"10.1016\/j.jim.2023.113427_bb0135","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1046\/j.1365-3083.2003.01320.x","article-title":"Monoclonal antibodies inducing conformational changes on the antigen molecule","volume":"58","author":"Roguin","year":"2003","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0140","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1016\/S0140-6736(07)61261-6","article-title":"Maternal and neonatal tetanus","volume":"370","author":"Roper","year":"2007","journal-title":"Lancet"},{"key":"10.1016\/j.jim.2023.113427_bb0145","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.toxicon.2012.12.027","article-title":"Tetanus neurotoxin","volume":"66","author":"Rossetto","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.jim.2023.113427_bb0150","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0022-2836(02)01403-1","article-title":"Two carbohydrate binding sites in the HCC-domain of tetanus neurotoxin are required for toxicity","volume":"326","author":"Rummel","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jim.2023.113427_bb0155","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1016\/j.molimm.2010.02.020","article-title":"Characterisation of a panel of anti-tetanus toxin single-chain Fvs reveals cooperative binding","volume":"47","author":"Scott","year":"2010","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0160","first-page":"161","article-title":"Development of a monoclonal antibody to tetanus toxin C fragment","volume":"25","author":"Shan","year":"2009","journal-title":"Chin. J. Cell Mol. Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0165","doi-asserted-by":"crossref","first-page":"4467","DOI":"10.1111\/j.1742-4658.2011.08183.x","article-title":"Molecular structures and functional relationships in clostridial neurotoxins","volume":"278","author":"Swaminathan","year":"2011","journal-title":"FEBS J."},{"key":"10.1016\/j.jim.2023.113427_bb0170","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/S0140-6736(14)60236-1","article-title":"Maternal and neonatal tetanus","volume":"385","author":"Thwaites","year":"2015","journal-title":"Lancet"},{"key":"10.1016\/j.jim.2023.113427_bb0175","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1128\/iai.45.3.604-609.1984","article-title":"Neutralization of tetanus toxin by distinct monoclonal antibodies binding to multiple epitopes on the toxin molecule","volume":"45","author":"Volk","year":"1984","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jim.2023.113427_bb0180","doi-asserted-by":"crossref","first-page":"266","DOI":"10.3390\/toxins8090266","article-title":"Tetanus neurotoxin neutralizing antibodies screened from a human immune scFv antibody phage display library","volume":"8","author":"Wang","year":"2016","journal-title":"Toxins"},{"key":"10.1016\/j.jim.2023.113427_bb0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.109070","article-title":"Structural basis of tetanus toxin neutralization by native human monoclonal antibodies","volume":"35","author":"Wang","year":"2021","journal-title":"Cell Rep."},{"key":"10.1016\/j.jim.2023.113427_bb0190","first-page":"42","article-title":"Analysis of morbidity trend of infectious diseases in Southeast Asia along the China-Proposed Belt and road initiative","volume":"32","author":"Xie","year":"2019","journal-title":"Med. Soc."},{"key":"10.1016\/j.jim.2023.113427_bb0195","first-page":"4","article-title":"Expression, purification and functional identification of the humanized monoclonal antibody against ScFv of tetanuss toxin","volume":"37","author":"Xiong","year":"2009","journal-title":"Prog. Microbiol. Immunol."},{"key":"10.1016\/j.jim.2023.113427_bb0200","first-page":"29","article-title":"Preparation and identification of monoclonal antibody against botulinum toxin serotype F","volume":"30","author":"Xu","year":"2020","journal-title":"Lett. Biotechnol."},{"key":"10.1016\/j.jim.2023.113427_bb0205","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001207","article-title":"SV2 mediates entry of tetanus neurotoxin into central neurons","volume":"6","author":"Yeh","year":"2010","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jim.2023.113427_bb0210","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1016\/S0140-6736(18)33131-3","volume":"393","author":"Yen","year":"2019","journal-title":"Tetanus. Lancet"},{"key":"10.1016\/j.jim.2023.113427_bb0215","doi-asserted-by":"crossref","first-page":"344","DOI":"10.4161\/hv.26769","article-title":"Comparative in vitro and in vivo assessment of toxin neutralization by anti-tetanus toxin monoclonal antibodies","volume":"10","author":"Yousefi","year":"2014","journal-title":"Hum. Vaccin. Immunother."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175923000091?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175923000091?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:37:44Z","timestamp":1758299864000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175923000091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2]]},"references-count":42,"alternative-id":["S0022175923000091"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2023.113427","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2023,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2023.113427","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"113427"}}