{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:17:05Z","timestamp":1789060625482,"version":"build-2803163510"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon 2020","doi-asserted-by":"publisher","award":["825747"],"award-info":[{"award-number":["825747"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jim.2025.113998","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:45:37Z","timestamp":1761723937000},"page":"113998","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Advancing cervical cancer screening: A novel in-house ELISA assay for the simultaneous detection of p16 and Ki-67"],"prefix":"10.1016","volume":"545","author":[{"given":"Sallam","family":"Al-Madhagi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Khashayar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieselotte","family":"Volckaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Vanfleteren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieke","family":"Adriaens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2025.113998_bb0005","author":"Abbexa"},{"key":"10.1016\/j.jim.2025.113998_bb0010","author":"Abcam"},{"key":"10.1016\/j.jim.2025.113998_bb0015","author":"Abcam"},{"key":"10.1016\/j.jim.2025.113998_bb0020","author":"Antibodies-online"},{"issue":"11","key":"10.1016\/j.jim.2025.113998_bb0025","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1158\/1055-9965.EPI-09-0328","article-title":"Evaluation of an ELISA for p16INK4a as a screening test for cervical cancer","volume":"18","author":"Balasubramanian","year":"2009","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"issue":"6","key":"10.1016\/j.jim.2025.113998_bb0030","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/cncy.21542","article-title":"Prospective evaluation of p16\/Ki-67 dual-stained cytology for managing women with abnormal Papanicolaou cytology: PALMS study results","volume":"123","author":"Bergeron","year":"2015","journal-title":"Cancer Cytopathol."},{"key":"10.1016\/j.jim.2025.113998_bb0035","author":"Biomatik"},{"issue":"2","key":"10.1016\/j.jim.2025.113998_bb0040","doi-asserted-by":"crossref","DOI":"10.3802\/jgo.2016.27.e21","article-title":"Clinical significance of human papillomavirus genotyping","volume":"27","author":"Choi","year":"2016","journal-title":"J. Gynecol. Oncol."},{"key":"10.1016\/j.jim.2025.113998_bb0045","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.cca.2015.03.005","article-title":"Cervical cancer: biomarkers for diagnosis and treatment","volume":"445","author":"Dasari","year":"2015","journal-title":"Clin. Chim. Acta"},{"issue":"1","key":"10.1016\/j.jim.2025.113998_bb0050","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s13027-020-0278-x","article-title":"HPV E6\/E7 mRNA test for the detection of high grade cervical intraepithelial neoplasia (CIN2+): a systematic review","volume":"15","author":"Derbie","year":"2020","journal-title":"Infect. Agent Cancer."},{"issue":"2","key":"10.1016\/j.jim.2025.113998_bb0060","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/aogs.12032","article-title":"Enzyme-linked immunosorbent assay for p16INK4a - a new triage test for the detection of cervical intraepithelial neoplasia?","volume":"92","author":"Jentschke","year":"2013","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"10.1016\/j.jim.2025.113998_bb0065","series-title":"Human p16-INK4a-TAT","first-page":"19","author":"Kit EC","year":"2025"},{"issue":"1","key":"10.1016\/j.jim.2025.113998_bb0070","doi-asserted-by":"crossref","DOI":"10.1111\/aji.13394","article-title":"Triaging abnormal cervical cancer screening tests using p16INK4a detection by ELISA on fresh cervical samples","volume":"86","author":"Leung","year":"2021","journal-title":"Am. J. Reprod. Immunol."},{"issue":"3","key":"10.1016\/j.jim.2025.113998_bb0075","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.3892\/mmr.2014.2914","article-title":"Predic Ki67 is a promising molecular target in the diagnosis of cancer (review)","volume":"11","author":"Li","year":"2015","journal-title":"Mol. Med. Rep."},{"issue":"11","key":"10.1016\/j.jim.2025.113998_bb0080","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1002\/ijc.22612","article-title":"Evaluation of a new p16INK4a ELISA test and a high-risk HPV DNA test for cervical cancer screening: results from proof-of-concept study","volume":"120","author":"Mao","year":"2007","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.jim.2025.113998_bb0085","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.cca.2019.01.011","article-title":"Ki-67 protein as a tumour proliferation marker","volume":"491","author":"Menon","year":"2019","journal-title":"Clin. Chim. Acta"},{"issue":"3","key":"10.1016\/j.jim.2025.113998_bb0100","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1002\/cncy.22103","article-title":"Meta-analysis of the accuracy of p16 or p16\/Ki-67 immunocytochemistry versus HPV testing for the detection of CIN2+\/CIN3+ in triage of women with minor abnormal cytology","volume":"127","author":"Peeters","year":"2019","journal-title":"Cancer Cytopathol."},{"key":"10.1016\/j.jim.2025.113998_bb0105","doi-asserted-by":"crossref","first-page":"200215","DOI":"10.1016\/j.tvr.2021.200215","article-title":"Corrigendum to CINtec PLUS and cobas HPV testing for triaging Canadian women referred to colposcopy with a history of low-grade squamous intraepithelial lesion: Baseline findings [Papillomavirus Res. 10 (2020) 100206]","author":"Ratnam","year":"2021","journal-title":"Tumour Virus Res."},{"issue":"18","key":"10.1016\/j.jim.2025.113998_bb0110","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1038\/onc.2010.614","article-title":"p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors","volume":"30","author":"Romagosa","year":"2011","journal-title":"Oncogene"},{"key":"10.1016\/j.jim.2025.113998_bb0115","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/pst.62","article-title":"Defining the linear portion of a sigmoidal curve: bend points","volume":"2","author":"Sebaugh","year":"2003","journal-title":"Pharm. Stat."},{"issue":"7","key":"10.1016\/j.jim.2025.113998_bb0120","doi-asserted-by":"crossref","first-page":"C98","DOI":"10.12968\/hmed.2016.77.7.C98","article-title":"Enzyme-linked immunosorbent assay (ELISA): the basics","volume":"77","author":"Shah","year":"2016","journal-title":"Br. J. Hosp. Med."},{"issue":"2","key":"10.1016\/j.jim.2025.113998_bb0125","first-page":"319","article-title":"Cervical cancer prevalence, incidence and mortality in low- and middle-income countries: a systematic review","volume":"19","author":"Shrestha","year":"2018","journal-title":"Asian Pac. J. Cancer Prev."},{"issue":"12","key":"10.1016\/j.jim.2025.113998_bb0130","doi-asserted-by":"crossref","DOI":"10.1093\/jnci\/djv257","article-title":"p16\/Ki-67 dual stain cytology for detection of cervical precancer in HPV-positive women","volume":"107","author":"Wentzensen","year":"2015","journal-title":"J. Natl. Cancer Inst."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217592500198X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002217592500198X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:34:33Z","timestamp":1773308073000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002217592500198X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":23,"alternative-id":["S002217592500198X"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2025.113998","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Advancing cervical cancer screening: A novel in-house ELISA assay for the simultaneous detection of p16 and Ki-67","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2025.113998","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"113998"}}