{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:54:55Z","timestamp":1785884095160,"version":"3.56.0"},"reference-count":11,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000016","name":"U.S. Department of Health and Human Services","doi-asserted-by":"publisher","award":["75N91019D00024"],"award-info":[{"award-number":["75N91019D00024"]}],"id":[{"id":"10.13039\/100000016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NIH NCI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.jim.2026.114075","type":"journal-article","created":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T22:04:41Z","timestamp":1780869881000},"page":"114075","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A semi-automated ELISA workflow for SARS-CoV-2 Nucleocapsid IgG detection significantly improves throughput and reduces hands-on time"],"prefix":"10.1016","volume":"549","author":[{"given":"Ruturaj R.","family":"Masvekar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Mansano","family":"Do Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliyah Hannah A.","family":"Remoroza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy J.","family":"Kemp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce K.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligia A.","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2026.114075_bb0005","series-title":"StatPearls","article-title":"Enzyme linked immunosorbent assay","author":"Alhajj","year":"2025"},{"key":"10.1016\/j.jim.2026.114075_bb0010","series-title":"Automated Avian Influenza ELISA with Agilent BioTek 406 FX Washer Dispenser","author":"Brescia","year":"2023"},{"issue":"5","key":"10.1016\/j.jim.2026.114075_bb0015","article-title":"Longitudinal assessment of BNT162b2- and mRNA-1273-induced anti-SARS-CoV-2 spike IgG levels and avidity following three doses of vaccination","volume":"12","author":"Bullock","year":"2024","journal-title":"Vaccines (Basel)"},{"issue":"2","key":"10.1016\/j.jim.2026.114075_bb0020","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.4301","article-title":"A high-throughput automated ELISA assay for detection of IgG antibodies to the SARS-CoV-2 spike protein","volume":"12","author":"Conkright-Fincham","year":"2022","journal-title":"Bio-Protoc."},{"issue":"11","key":"10.1016\/j.jim.2026.114075_bb0025","doi-asserted-by":"crossref","DOI":"10.1128\/msphere.00393-24","article-title":"Analytical measuring interval, linearity, and precision of serology assays for detection of SARS-CoV-2 antibodies according to CLSI guidelines","volume":"9","author":"Haynesworth","year":"2024","journal-title":"mSphere"},{"issue":"2","key":"10.1016\/j.jim.2026.114075_bb0030","doi-asserted-by":"crossref","first-page":"2215677","DOI":"10.1080\/21645515.2023.2215677","article-title":"SARS-CoV-2 IgG Spike antibody levels and avidity in natural infection or following vaccination with mRNA-1273 or BNT162b2 vaccines","volume":"19","author":"Hickey","year":"2023","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.jim.2026.114075_bb0035","article-title":"Enzyme-linked immunosorbent assays","author":"Hornbeck","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"issue":"1\u20132","key":"10.1016\/j.jim.2026.114075_bb0040","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0022-1759(92)90061-W","article-title":"Enzyme immunoassay techniques. An overview","volume":"150","author":"Porstmann","year":"1992","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2026.114075_bb0045","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.jpba.2012.05.027","article-title":"Full automation and validation of a flexible ELISA platform for host cell protein and protein A impurity detection in biopharmaceuticals","volume":"70","author":"Rey","year":"2012","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"1","key":"10.1016\/j.jim.2026.114075_bb0050","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.slast.2021.10.012","article-title":"A collaborative robotic solution to partly automate SARS-CoV-2 serological tests in small facilities","volume":"27","author":"Zanchettin","year":"2022","journal-title":"SLAS Technol."},{"issue":"33","key":"10.1016\/j.jim.2026.114075_bb0055","doi-asserted-by":"crossref","first-page":"11424","DOI":"10.1021\/acs.analchem.1c01231","article-title":"Sample-to-answer robotic ELISA","volume":"93","author":"Zhou","year":"2021","journal-title":"Anal. Chem."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000517?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000517?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:34:14Z","timestamp":1785882854000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175926000517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":11,"alternative-id":["S0022175926000517"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2026.114075","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A semi-automated ELISA workflow for SARS-CoV-2 Nucleocapsid IgG detection significantly improves throughput and reduces hands-on time","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2026.114075","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114075"}}