{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T13:15:49Z","timestamp":1785244549492,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["101080692"],"award-info":[{"award-number":["101080692"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF22SA0081890"],"award-info":[{"award-number":["NNF22SA0081890"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF24OC0096734"],"award-info":[{"award-number":["NNF24OC0096734"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF23SA0088562"],"award-info":[{"award-number":["NNF23SA0088562"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.jim.2026.114084","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T14:17:26Z","timestamp":1782656246000},"page":"114084","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters"],"prefix":"10.1016","volume":"549","author":[{"given":"Tolunay","family":"Ata\u00e7","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Sid","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Krag","family":"Isling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Kristian","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"W\u00f8rzner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7818","key":"10.1016\/j.jim.2026.114084_bb0005","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1038\/s41586-020-2312-y","article-title":"The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice","volume":"583","author":"Bao","year":"2020","journal-title":"Nature"},{"issue":"4","key":"10.1016\/j.jim.2026.114084_bb0010","doi-asserted-by":"crossref","DOI":"10.1111\/pim.12823","article-title":"Establishment of monoclonal antibodies to evaluate the cellular immunity in a hamster model of L infantum infection","volume":"43","author":"Carvalho","year":"2021","journal-title":"Parasite Immunol."},{"issue":"9","key":"10.1016\/j.jim.2026.114084_bb0015","doi-asserted-by":"crossref","first-page":"2428","DOI":"10.1093\/cid\/ciaa325","article-title":"Simulation of the clinical and pathological manifestations of coronavirus disease 2019 (COVID-19) in a Golden Syrian hamster model: implications for disease pathogenesis and transmissibility","volume":"71","author":"Chan","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jim.2026.114084_bb0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2022.104248","article-title":"Protection against SARS-CoV-2 transmission by a parenteral prime-intranasal boost vaccine strategy","volume":"84","author":"Christensen","year":"2022","journal-title":"EBioMedicine"},{"issue":"11","key":"10.1016\/j.jim.2026.114084_bb0025","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1002\/cyto.a.20435","article-title":"TCR and CD3 antibody cross-reactivity in 44 species","volume":"71","author":"Conrad","year":"2007","journal-title":"Cytometry A"},{"issue":"1\u20132","key":"10.1016\/j.jim.2026.114084_bb0030","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.bbamem.2005.10.011","article-title":"Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. Tuberculosis (trehalose 6,6\u2032-dibehenate)-a novel adjuvant inducing both strong CMI and antibody responses","volume":"1718","author":"Davidsen","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"issue":"8","key":"10.1016\/j.jim.2026.114084_bb0035","doi-asserted-by":"crossref","DOI":"10.3390\/v14081629","article-title":"Influenza viruses suitable for studies in Syrian hamsters","volume":"14","author":"Fan","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0040","doi-asserted-by":"crossref","first-page":"5868","DOI":"10.1038\/s41467-021-26178-y","article-title":"ChAdOx1 nCoV-19 (AZD1222) protects Syrian hamsters against SARS-CoV-2 B.1.351 and B.1.1.7","volume":"12","author":"Fischer","year":"2021","journal-title":"Nat. Commun."},{"issue":"19","key":"10.1016\/j.jim.2026.114084_bb0045","doi-asserted-by":"crossref","first-page":"9929","DOI":"10.1128\/JVI.05356-11","article-title":"T cells are not required for pathogenesis in the Syrian hamster model of hantavirus pulmonary syndrome","volume":"85","author":"Hammerbeck","year":"2011","journal-title":"J. Virol."},{"issue":"66","key":"10.1016\/j.jim.2026.114084_bb0050","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abm3131","article-title":"Immune memory from SARS-CoV-2 infection in hamsters provides variant-independent protection but still allows virus transmission","volume":"6","author":"Horiuchi","year":"2021","journal-title":"Sci. Immunol."},{"issue":"28","key":"10.1016\/j.jim.2026.114084_bb0055","doi-asserted-by":"crossref","first-page":"16587","DOI":"10.1073\/pnas.2009799117","article-title":"Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development","volume":"117","author":"Imai","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"10.1016\/j.jim.2026.114084_bb0060","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01693-17","article-title":"Syrian Hamster as an animal model for the study of human influenza virus infection","volume":"92","author":"Iwatsuki-Horimoto","year":"2018","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.jim.2026.114084_bb0065","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.4049\/jimmunol.150.12.5429","article-title":"Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies","volume":"150","author":"Jones","year":"1993","journal-title":"J. Immunol."},{"issue":"11","key":"10.1016\/j.jim.2026.114084_bb0070","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1111\/pim.12358","article-title":"Induction of regulatory T cells by Opisthorchis viverrini","volume":"38","author":"Kaewraemruaen","year":"2016","journal-title":"Parasite Immunol."},{"issue":"7821","key":"10.1016\/j.jim.2026.114084_bb0075","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/s41586-020-2550-z","article-title":"SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS, and uninfected controls","volume":"584","author":"Le Bert","year":"2020","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0080","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/s41541-024-00992-z","article-title":"High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters","volume":"9","author":"Lett","year":"2024","journal-title":"NPJ Vaccines."},{"key":"10.1016\/j.jim.2026.114084_bb0085","series-title":"Flow Cytometry Protocols","first-page":"151","article-title":"Multiparameter intracellular cytokine staining","author":"Lovelace","year":"2018"},{"issue":"1\u20132","key":"10.1016\/j.jim.2026.114084_bb0090","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0022-1759(01)00416-1","article-title":"Use of overlapping peptide mixtures as antigens for cytokine flow cytometry","volume":"255","author":"Maecker","year":"2001","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2026.114084_bb0095","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.3389\/fimmu.2019.02329","article-title":"Syrian Hamster as an animal model for the study on infectious diseases","volume":"10","author":"Miao","year":"2019","journal-title":"Front. Immunol."},{"issue":"4","key":"10.1016\/j.jim.2026.114084_bb0100","first-page":"265","article-title":"Hamster IFN-\u03b3+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice","volume":"36","author":"Nakornpakdee","year":"2018","journal-title":"Asian Pac. J. Allergy Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0105","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ejpb.2010.10.001","article-title":"Incorporation of a synthetic mycobacterial monomycoloyl glycerol analogue stabilizes dimethyldioctadecylammonium liposomes and potentiates their adjuvant effect in vivo","volume":"77","author":"Nordly","year":"2011","journal-title":"Eur. J. Pharm. Biopharm."},{"issue":"5","key":"10.1016\/j.jim.2026.114084_bb0110","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/s41564-023-01352-8","article-title":"Live-attenuated vaccine sCPD9 elicits superior mucosal and systemic immunity to SARS-CoV-2 variants in hamsters","volume":"8","author":"Nouailles","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.jim.2026.114084_bb0115","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.smim.2018.10.003","article-title":"Immunocorrelates of CAF family adjuvants","volume":"39","author":"Pedersen","year":"2018","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.jim.2026.114084_bb0120","doi-asserted-by":"crossref","DOI":"10.1186\/s12951-026-04234-3","article-title":"A stable two-component cationic liposome platform for mRNA delivery induces CD8(+) T-cell responses and protection in a murine lymphoma model","volume":"24","author":"Pedersen","year":"2026","journal-title":"J. Nanobiotechnol."},{"issue":"2","key":"10.1016\/j.jim.2026.114084_bb0125","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/imm.12116","article-title":"The adaptive immune response does not influence hantavirus disease or persistence in the Syrian hamster","volume":"140","author":"Prescott","year":"2013","journal-title":"Immunology"},{"key":"10.1016\/j.jim.2026.114084_bb0130","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.vetimm.2016.12.003","article-title":"Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules","volume":"183","author":"Rees","year":"2017","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"10","key":"10.1016\/j.jim.2026.114084_bb0135","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.3201\/eid2910.230544","article-title":"Human-to-human transmission of Andes virus Modeled in Syrian hamsters","volume":"29","author":"Riesle-Sbarbaro","year":"2023","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0140","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1186\/s12903-025-06712-w","article-title":"Immunological balance skewing Th1,Th17 towards Th2,Treg in OSCC of hamster induced by 7,12- dimethylbenz[a]anthracene","volume":"25","author":"Shao","year":"2025","journal-title":"BMC Oral Health"},{"issue":"7818","key":"10.1016\/j.jim.2026.114084_bb0145","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1038\/s41586-020-2342-5","article-title":"Pathogenesis and transmission of SARS-CoV-2 in golden hamsters","volume":"583","author":"Sia","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jim.2026.114084_bb0150","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1514845","article-title":"Adjuvanted subunit intranasal vaccine reduces SARS-CoV-2 onward transmission in hamsters","volume":"16","author":"Sui","year":"2025","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0155","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.chom.2020.05.020","article-title":"A mouse model of SARS-CoV-2 infection and pathogenesis","volume":"28","author":"Sun","year":"2020","journal-title":"Cell Host Microbe"},{"issue":"3","key":"10.1016\/j.jim.2026.114084_bb0160","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.bpj.2017.12.011","article-title":"The effect of fluorophore conjugation on antibody affinity and the photophysical properties of dyes","volume":"114","author":"Szab\u00f3","year":"2018","journal-title":"Biophys. J."},{"key":"10.1016\/j.jim.2026.114084_bb0165","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.979641","article-title":"Intranasal delivery of a chimpanzee adenovirus vector expressing a pre-fusion spike (BV-AdCoV-1) protects golden Syrian hamsters against SARS-CoV-2 infection","volume":"12","author":"Wang","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.jim.2026.114084_bb0170","series-title":"Emerging and re-Emerging Viral Infections: Advances in Microbiology, Infectious Diseases and Public Health","first-page":"87","article-title":"Syrian hamsters as a small animal model for emerging infectious diseases: Advances in immunologic methods","volume":"6","author":"Warner","year":"2017"},{"issue":"12","key":"10.1016\/j.jim.2026.114084_bb0175","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0001432","article-title":"Nipah virus transmission in a hamster model","volume":"5","author":"de Wit","year":"2011","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.jim.2026.114084_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2025.105615","article-title":"Intranasal recombinant protein subunit vaccine targeting TLR3 induces respiratory tract IgA and CD8 T cell responses and protects against respiratory virus infection","volume":"113","author":"W\u00f8rzner","year":"2025","journal-title":"EBioMedicine"},{"issue":"1","key":"10.1016\/j.jim.2026.114084_bb0185","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009195","article-title":"K18-hACE2 mice develop respiratory disease resembling severe COVID-19","volume":"17","author":"Yinda","year":"2021","journal-title":"PLoS Pathog."},{"issue":"5\u20136","key":"10.1016\/j.jim.2026.114084_bb0190","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.cyto.2006.05.006","article-title":"Biological activity of hamster interferon-gamma is modulated by the carboxyl-terminal tail","volume":"34","author":"Zhao","year":"2006","journal-title":"Cytokine"},{"issue":"1\u20132","key":"10.1016\/j.jim.2026.114084_bb0195","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jim.2011.02.004","article-title":"Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus)","volume":"368","author":"Zivcec","year":"2011","journal-title":"J. Immunol. Methods"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000608?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000608?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:54:33Z","timestamp":1785243273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175926000608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":39,"alternative-id":["S0022175926000608"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2026.114084","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2026.114084","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114084"}}