{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:18:39Z","timestamp":1790507919036,"version":"4.1.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.jim.2026.114086","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T14:51:19Z","timestamp":1783781479000},"page":"114086","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Allotypes matter: Evaluation of twelve commercially available antihuman IgG2 antibodies for subclass specificity"],"prefix":"10.1016","volume":"549","author":[{"given":"Theo","family":"Rispens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ninotska","family":"Derksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"van Mierlo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Keijzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"de Taeye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jim.2026.114086_bb0005","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0014-5793(93)81108-C","article-title":"Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin","volume":"322","author":"Bagci","year":"1993","journal-title":"FEBS Lett."},{"issue":"6504","key":"10.1016\/j.jim.2026.114086_bb0010","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1126\/science.abc5902","article-title":"Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability","volume":"369","author":"Brouwer","year":"2020","journal-title":"Science"},{"issue":"2","key":"10.1016\/j.jim.2026.114086_bb0015","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0090-1229(87)90129-2","article-title":"IgG subclass potential of surface IgM-negative and surface IgM-positive human peripheral blood B cells","volume":"43","author":"Conley","year":"1987","journal-title":"Clin. Immunol. Immunopathol."},{"key":"10.1016\/j.jim.2026.114086_bb0020","article-title":"Impact of structural modifications of IgG antibodies on effector functions","volume":"14","author":"Damelang","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2026.114086_bb0025","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.00740","article-title":"FcgammaR binding and ADCC activity of human IgG allotypes","volume":"11","author":"de Taeye","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jim.2026.114086_bb0030","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1038\/s41590-024-01916-8","article-title":"A unique serum IgG glycosylation signature predicts development of Crohn\u2019s disease and is associated with pathogenic antibodies to mannose glycan","volume":"25","author":"Gaifem","year":"2024","journal-title":"Nat. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114086_bb0035","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0022-1759(93)90263-7","article-title":"Epitope mapping of human immunoglobulin-specific murine monoclonal antibodies with domain-switched, deleted and point-mutated chimeric antibodies","volume":"158","author":"Hamilton","year":"1993","journal-title":"J. Immunol. Methods"},{"issue":"7","key":"10.1016\/j.jim.2026.114086_bb0040","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1111\/j.1348-0421.1989.tb02008.x","article-title":"Evaluation of production and characterization of monoclonal antibodies to human IgG of four subclasses","volume":"33","author":"Harada","year":"1989","journal-title":"Microbiol. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114086_bb0045","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0022-1759(91)90213-Y","article-title":"Identification of epitopes recognized by a panel of six anti-human IgG2 monoclonal antibodies","volume":"141","author":"Harada","year":"1991","journal-title":"J. Immunol. Methods"},{"issue":"2","key":"10.1016\/j.jim.2026.114086_bb0050","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0161-5890(92)90095-F","article-title":"Hinge region of human IgG2 protein: conformational studies with monoclonal antibodies","volume":"29","author":"Harada","year":"1992","journal-title":"Mol. Immunol."},{"issue":"D1","key":"10.1016\/j.jim.2026.114086_bb0055","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkad1049","article-title":"Ensembl 2024","volume":"52","author":"Harrison","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"12","key":"10.1016\/j.jim.2026.114086_bb0060","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1111\/trf.13812","article-title":"Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent","volume":"56","author":"Howie","year":"2016","journal-title":"Transfusion (Paris)"},{"issue":"5","key":"10.1016\/j.jim.2026.114086_bb0065","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1111\/trf.15796","article-title":"Characterization and refinement of monoclonal anti-human globulins that lack reactivity with human IgG4","volume":"60","author":"Howie","year":"2020","journal-title":"Transfusion (Paris)"},{"issue":"3\u20134","key":"10.1016\/j.jim.2026.114086_bb0070","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0165-2478(85)90082-3","article-title":"Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS\/WHO collaborative study","volume":"10","author":"Jefferis","year":"1985","journal-title":"Immunol. Lett."},{"issue":"2","key":"10.1016\/j.jim.2026.114086_bb0075","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0165-2478(92)90141-A","article-title":"Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS\/WHO collaborative study","volume":"31","author":"Jefferis","year":"1992","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.jim.2026.114086_bb0080","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/S0076-6879(97)79049-9","article-title":"Preparation and properties of anti-biotin antibodies","volume":"279","author":"Kohen","year":"1997","journal-title":"Methods Enzymol."},{"issue":"9","key":"10.1016\/j.jim.2026.114086_bb0085","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines8090319","article-title":"Immunoglobulins or antibodies: IMGT((R)) bridging genes, structures and functions","volume":"8","author":"Lefranc","year":"2020","journal-title":"Biomedicines"},{"key":"10.1016\/j.jim.2026.114086_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2024.113766","article-title":"The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG1","volume":"534","author":"Moverare","year":"2024","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2026.114086_bb0095","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1021016","article-title":"Association of dengue infection with anti-alpha-gal antibodies, IgM, IgG, IgG1, and IgG2","volume":"13","author":"Olajiga","year":"2022","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114086_bb0100","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4049\/jimmunol.2101196","article-title":"At critically low antigen densities, IgM hexamers outcompete both IgM pentamers and IgG1 for human complement deposition and complement-dependent cytotoxicity","volume":"209","author":"Oskam","year":"2022","journal-title":"J. Immunol."},{"issue":"3","key":"10.1016\/j.jim.2026.114086_bb0105","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1089\/hyb.1984.3.263","article-title":"Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes","volume":"3","author":"Reimer","year":"1984","journal-title":"Hybridoma"},{"issue":"11","key":"10.1016\/j.jim.2026.114086_bb0110","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/s41577-023-00871-z","article-title":"The unique properties of IgG4 and its roles in health and disease","volume":"23","author":"Rispens","year":"2023","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jim.2026.114086_bb0115","doi-asserted-by":"crossref","unstructured":"Rispens T, Hart MH, Ooijevaar-de HP, van LA, Vennegoor A, Killestein J, et al. Drug interference in immunogenicity assays depends on valency. J. Pharm. Biomed. Anal. 2013;85:179\u201385.","DOI":"10.1016\/j.jpba.2013.07.022"},{"issue":"269","key":"10.1016\/j.jim.2026.114086_bb0120","doi-asserted-by":"crossref","first-page":"269ra1","DOI":"10.1126\/scitranslmed.3010524","article-title":"The human IgG anti-carbohydrate repertoire exhibits a universal architecture and contains specificity for microbial attachment sites","volume":"7","author":"Schneider","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"5","key":"10.1016\/j.jim.2026.114086_bb0125","doi-asserted-by":"crossref","DOI":"10.1002\/cti2.1285","article-title":"Dynamics of antibodies to SARS-CoV-2 in convalescent plasma donors","volume":"10","author":"Steenhuis","year":"2021","journal-title":"Clin. Transl. Immunol."},{"issue":"7","key":"10.1016\/j.jim.2026.114086_bb0130","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1111\/all.16089","article-title":"Suppressed IgG4 class switching in dupilumab- and TNF inhibitor-treated patients after mRNA vaccination","volume":"79","author":"Valk","year":"2024","journal-title":"Allergy"},{"key":"10.1016\/j.jim.2026.114086_bb0140","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3389\/fimmu.2014.00520","article-title":"IgG subclasses and allotypes: from structure to effector functions","volume":"5","author":"Vidarsson","year":"2014","journal-title":"Front. Immunol."},{"issue":"5","key":"10.1016\/j.jim.2026.114086_bb0145","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.jaci.2022.05.023","article-title":"Comprehensive overview of autoantibody isotype and subclass distribution","volume":"150","author":"Volkov","year":"2022","journal-title":"J. Allergy Clin. Immunol."},{"issue":"9","key":"10.1016\/j.jim.2026.114086_bb0150","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1084\/jem.20132444","article-title":"Increased severity of respiratory infections associated with elevated anti-LPS IgG2 which inhibits serum bactericidal killing","volume":"211","author":"Wells","year":"2014","journal-title":"J. Exp. Med."},{"issue":"3","key":"10.1016\/j.jim.2026.114086_bb0155","first-page":"180","article-title":"Clinical bacteriology and immunology in acute otitis media in children","volume":"14","author":"Yamanaka","year":"2008","journal-title":"J. Inf. Secur."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000621?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000621?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:55:07Z","timestamp":1785243307000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175926000621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":30,"alternative-id":["S0022175926000621"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2026.114086","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Allotypes matter: Evaluation of twelve commercially available antihuman IgG2 antibodies for subclass specificity","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2026.114086","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114086"}}