{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:13:16Z","timestamp":1788556396217,"version":"build-2803163510"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408682\/2022-1"],"award-info":[{"award-number":["408682\/2022-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010629","name":"Fulbright Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010629","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-01499-21"],"award-info":[{"award-number":["APQ-01499-21"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-04272-25"],"award-info":[{"award-number":["APQ-04272-25"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jim.2026.114089","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:32:48Z","timestamp":1784763168000},"page":"114089","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Detection of SARS-CoV-2 epitope-specific CD8+ T cell responses in convalescent COVID individuals using HLA-A*02 dimers"],"prefix":"10.1016","volume":"550","author":[{"given":"\u00c1gata","family":"Lopes-Ribeiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00edza Aline","family":"Pereira-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eduarda Martuchele","family":"F\u00e9lix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Cardoso","family":"Corr\u00eaa-Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olindo Assis","family":"Martins-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Peruhype-Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a","family":"Teixeira-Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moriya","family":"Tsuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana Grazziela","family":"Coelho-dos-Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jim.2026.114089_bb0005","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2023.866407","article-title":"An HLA map of the world: a comparison of HLA frequencies in 200 worldwide populations reveals diverse patterns for class I and class II","volume":"14","author":"Arrieta-Bola\u00f1os","year":"2023","journal-title":"Front. Genet."},{"issue":"7","key":"10.1016\/j.jim.2026.114089_bb0010","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1038\/s41591-021-01386-7","article-title":"CD8+ T cells contribute to survival in patients with COVID-19 and hematologic cancer","volume":"27","author":"Bange","year":"2021","journal-title":"Nat. Med."},{"key":"10.1016\/j.jim.2026.114089_bb0015","unstructured":"BD Biosciences. (n.d.). \u201cDimerX I: Recombinant Soluble Dimeric Human HLA-A2:Ig Fusion Protein\u201d. Technical data sheet (Material no. 551263, Rev. 1). Retrieved December 15, 2025. Available from https:\/\/www.bdbiosciences.com\/content\/dam\/bdb\/product_assets\/product_pdf\/proteinproduct\/pdf_0\/551263.pdf."},{"issue":"1\u20132","key":"10.1016\/j.jim.2026.114089_bb0020","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.imlet.2013.11.005","article-title":"Proximity of TCR and its CD8 coreceptor controls sensitivity of T cells","volume":"157","author":"Borger","year":"2014","journal-title":"Immunol. Lett."},{"issue":"10","key":"10.1016\/j.jim.2026.114089_bb0025","doi-asserted-by":"crossref","first-page":"4866","DOI":"10.4049\/jimmunol.1200402","article-title":"Antigen-independent differentiation and maintenance of effector-like resident memory T cells in tissues","volume":"188","author":"Casey","year":"2012","journal-title":"The Journal of Immunology"},{"key":"10.1016\/j.jim.2026.114089_bb0030","article-title":"Immunology of viral infections with a high impact in Mexico: dengue, chikungunya, and Zika","author":"Castillo-Mac\u00edas","year":"2018","journal-title":"Med. Univ."},{"issue":"6","key":"10.1016\/j.jim.2026.114089_bb0035","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1002\/eji.201646837","article-title":"CD69: from activation marker to metabolic gatekeeper","volume":"47","author":"Cibri\u00e1n","year":"2017","journal-title":"Eur. J. Immunol."},{"issue":"6","key":"10.1016\/j.jim.2026.114089_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006462","article-title":"Multi-parameter approach to evaluate the timing of memory status after 17DD-YF primary vaccination","volume":"12","author":"Costa-Pereira","year":"2018","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"9","key":"10.1016\/j.jim.2026.114089_bb0045","doi-asserted-by":"crossref","first-page":"4771","DOI":"10.1128\/JVI.02892-15","article-title":"Immunodominant dengue virus-specific CD8 + T cell responses are associated with a memory PD-1 + phenotype","volume":"90","author":"de Alwis","year":"2016","journal-title":"J. Virol."},{"issue":"4","key":"10.1016\/j.jim.2026.114089_bb0050","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/imm.12992","article-title":"Human <scp>CD<\/scp> 8 T-cell activation in acute and chronic chikungunya infection","volume":"155","author":"Dias","year":"2018","journal-title":"Immunology"},{"issue":"2","key":"10.1016\/j.jim.2026.114089_bb0055","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/tan.12352","article-title":"The heterogeneous <scp>HLA<\/scp> genetic composition of the Brazilian population and its relevance to the optimization of hematopoietic stem cell donor recruitment","volume":"84","author":"Fabreti-Oliveira","year":"2014","journal-title":"Tissue Antigens"},{"issue":"6324","key":"10.1016\/j.jim.2026.114089_bb0060","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/351290a0","article-title":"Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules","volume":"351","author":"Falk","year":"1991","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0065","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1080\/14760584.2018.1406800","article-title":"The 17D-204 and 17DD yellow fever vaccines: an overview of major similarities and subtle differences","volume":"17","author":"Ferreira","year":"2018","journal-title":"Expert Rev. Vaccines"},{"issue":"4","key":"10.1016\/j.jim.2026.114089_bb0070","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/s41590-020-0607-7","article-title":"Developmental plasticity allows outside-in immune responses by resident memory T cells","volume":"21","author":"Fonseca","year":"2020","journal-title":"Nat. Immunol."},{"issue":"7","key":"10.1016\/j.jim.2026.114089_bb0075","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.chom.2021.05.010","article-title":"SARS-CoV-2 human T cell epitopes: adaptive immune response against COVID-19","volume":"29","author":"Grifoni","year":"2021","journal-title":"Cell Host Microbe"},{"issue":"27","key":"10.1016\/j.jim.2026.114089_bb0080","doi-asserted-by":"crossref","first-page":"4180","DOI":"10.3748\/wjg.v11.i27.4180","article-title":"Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes","volume":"11","author":"He","year":"2005","journal-title":"World J. Gastroenterol."},{"issue":"2","key":"10.1016\/j.jim.2026.114089_bb0085","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.cell.2005.05.013","article-title":"How the T cell repertoire becomes peptide and MHC specific","volume":"122","author":"Huseby","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.jim.2026.114089_bb0090","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1067463","article-title":"HLA variants have different preferences to present proteins with specific molecular functions which are complemented in frequent haplotypes","volume":"13","author":"Karnaukhov","year":"2022","journal-title":"Front. Immunol."},{"issue":"8","key":"10.1016\/j.jim.2026.114089_bb0095","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1177\/26.8.357645","article-title":"Steric hindrance as a factor in the reaction of labeled antibody with cell surface antigenic determinants","volume":"26","author":"Kent","year":"1978","journal-title":"Journal of Histochemistry & Cytochemistry"},{"issue":"3","key":"10.1016\/j.jim.2026.114089_bb0100","doi-asserted-by":"crossref","first-page":"361","DOI":"10.4049\/jimmunol.2300399","article-title":"CD8+ tissue-resident memory T cells: versatile guardians of the tissue","volume":"212","author":"Marchesini Tovar","year":"2024","journal-title":"J. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0105","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41514-019-0041-y","article-title":"CMV-independent increase in CD27\u2212CD28+ CD8+ EMRA T cells is inversely related to mortality in octogenarians","volume":"6","author":"Martin-Ruiz","year":"2020","journal-title":"npj Aging and Mechanisms of Disease"},{"key":"10.1016\/j.jim.2026.114089_bb0110","article-title":"Distinct CD8 + T-cell types associated with COVID-19 severity in unvaccinated HLA-A2 + patients","author":"Masuda","year":"2025","journal-title":"iScience"},{"issue":"11","key":"10.1016\/j.jim.2026.114089_bb0115","doi-asserted-by":"crossref","first-page":"7220","DOI":"10.4049\/jimmunol.179.11.7220","article-title":"Cutting edge: tissue-resident memory CTL down-regulate cytolytic molecule expression following virus clearance","volume":"179","author":"Mintern","year":"2007","journal-title":"The Journal of Immunology"},{"key":"10.1016\/j.jim.2026.114089_bb0120","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.86014","article-title":"Immune mechanisms underlying COVID-19 pathology and post-acute sequelae of SARS-CoV-2 infection (PASC)","volume":"12","author":"Mohandas","year":"2023","journal-title":"eLife"},{"key":"10.1016\/j.jim.2026.114089_bb0125","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2020.597627","article-title":"Coreceptors and TCR signaling \u2013 the strong and the weak of it","volume":"8","author":"M\u00f8rch","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"issue":"6","key":"10.1016\/j.jim.2026.114089_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.xcrm.2020.100081","article-title":"SARS-CoV-2-specific T cells exhibit phenotypic features of helper function, lack of terminal differentiation, and high proliferation potential","volume":"1","author":"Neidleman","year":"2020","journal-title":"Cell Reports Medicine"},{"issue":"13","key":"10.1016\/j.jim.2026.114089_bb0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.110167","article-title":"HLA-A\u221702:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses","volume":"37","author":"Nesterenko","year":"2021","journal-title":"Cell Rep."},{"issue":"11","key":"10.1016\/j.jim.2026.114089_bb0140","doi-asserted-by":"crossref","first-page":"5759","DOI":"10.1128\/JVI.00045-07","article-title":"Human immunodeficiency virus type 1 (HIV-1)-specific CD8 + T EMRA cells in early infection are linked to control of HIV-1 viremia and predict the subsequent viral load set point","volume":"81","author":"Northfield","year":"2007","journal-title":"J. Virol."},{"issue":"10","key":"10.1016\/j.jim.2026.114089_bb0145","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/icb.2016.60","article-title":"Distinct recirculation potential of CD69 + CD103 \u2212 and CD103 + thymic memory CD8 + T cells","volume":"94","author":"Park","year":"2016","journal-title":"Immunol. Cell Biol."},{"issue":"11","key":"10.1016\/j.jim.2026.114089_bb0150","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1038\/s41590-020-0782-6","article-title":"Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19","volume":"21","author":"Peng","year":"2020","journal-title":"Nat. Immunol."},{"issue":"65","key":"10.1016\/j.jim.2026.114089_bb0155","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abl9105","article-title":"SARS-CoV-2 infection generates tissue-localized immunological memory in humans","volume":"6","author":"Poon","year":"2021","journal-title":"Science Immunology"},{"issue":"4","key":"10.1016\/j.jim.2026.114089_bb0160","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.pathol.2021.09.005","article-title":"Cytotoxic CD8-positive T-lymphocyte infiltration in the lungs as a histological pattern of SARS-CoV-2 pneumonitis","volume":"54","author":"Puzyrenko","year":"2022","journal-title":"Pathology"},{"key":"10.1016\/j.jim.2026.114089_bb0165","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.764949","article-title":"CD8+ T-cell epitope variations suggest a potential antigen HLA-A2 binding deficiency for spike protein of SARS-CoV-2","volume":"12","author":"Qiu","year":"2022","journal-title":"Frontiers in Immunology"},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0170","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/JLB.5COVA0721-392RR","article-title":"Characterization of memory T cell subsets and common \u03b3\u2212chain cytokines in convalescent COVID-19 individuals","volume":"112","author":"Rajamanickam","year":"2022","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.jim.2026.114089_bb0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2021.112997","article-title":"A chimeric HLA-A2:\u03b22M:Ig fusion protein for the study of virus-specific CD8+ T-cells","volume":"492","author":"Ribeiro","year":"2021","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jim.2026.114089_bb0180","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1415914","article-title":"The role of circulating T cells with a tissue resident phenotype (ex-TRM) in health and disease","volume":"15","author":"Rodger","year":"2024","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0185","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1146\/annurev-immunol-032414-112334","article-title":"T cell antigen receptor recognition of antigen-presenting molecules","volume":"33","author":"Rossjohn","year":"2015","journal-title":"Annu. Rev. Immunol."},{"issue":"39","key":"10.1016\/j.jim.2026.114089_bb0190","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2411428121","article-title":"SARS-CoV-2-specific CD8 + T cells from people with long COVID establish and maintain effector phenotype and key TCR signatures over 2 years","volume":"121","author":"Rowntree","year":"2024","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0195","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-023-37368-1","article-title":"Post-acute sequelae of COVID-19 is characterized by diminished peripheral CD8+\u03b27 integrin+ T cells and anti-SARS-CoV-2 IgA response","volume":"14","author":"Santa Cruz","year":"2023","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.jim.2026.114089_bb0200","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/nri3152","article-title":"Expanding roles for CD4+ T cells in immunity to viruses","volume":"12","author":"Swain","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jim.2026.114089_bb0205","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2019.02125","article-title":"Human T cell response to dengue virus infection","volume":"10","author":"Tian","year":"2019","journal-title":"Front. Immunol."},{"issue":"2","key":"10.1016\/j.jim.2026.114089_bb0210","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.110214","article-title":"Recruitment of highly cytotoxic CD8+ T cell receptors in mild SARS-CoV-2 infection","volume":"38","author":"Wagner","year":"2022","journal-title":"Cell Rep."},{"issue":"11","key":"10.1016\/j.jim.2026.114089_bb0215","doi-asserted-by":"crossref","first-page":"5535","DOI":"10.1128\/JVI.78.11.5535-5545.2004","article-title":"Memory CD8 T-cell differentiation during viral infection","volume":"78","author":"Wherry","year":"2004","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.jim.2026.114089_bb0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2022.101206","article-title":"Production and purification of infectious diseases-associated tetramer for staining of CD8+ T cells from PBMCs","volume":"3","author":"Ye","year":"2022","journal-title":"STAR Protocols"},{"issue":"7685","key":"10.1016\/j.jim.2026.114089_bb0225","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/nature25144","article-title":"Effector CD8 T cells dedifferentiate into long-lived memory cells","volume":"552","author":"Youngblood","year":"2017","journal-title":"Nature"}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000657?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000657?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:27:34Z","timestamp":1788553654000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175926000657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":45,"alternative-id":["S0022175926000657"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2026.114089","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of SARS-CoV-2 epitope-specific CD8+ T cell responses in convalescent COVID individuals using HLA-A*02 dimers","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2026.114089","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114089"}}