{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:13:10Z","timestamp":1788556390759,"version":"build-2803163510"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunological Methods"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jim.2026.114105","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T23:38:13Z","timestamp":1786491493000},"page":"114105","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Isolation of region-specific factors driving antibody class-switch recombination from the immunoglobulin heavy chain locus"],"prefix":"10.1016","volume":"550","author":[{"given":"Santosh Kumar","family":"Gothwal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.jim.2026.114105_bb0005","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.1182\/blood.V98.10.3082","article-title":"Rearrangements of the c-myc oncogene are present in 15% of primary human multiple myeloma tumors","volume":"98","author":"Avet-Loiseau","year":"2001","journal-title":"Blood"},{"issue":"5967","key":"10.1016\/j.jim.2026.114105_bb0010","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1126\/science.1183439","article-title":"PRDM9 is a major determinant of meiotic recombination hotspots in humans and mice","volume":"327","author":"Baudat","year":"2010","journal-title":"Science"},{"issue":"7422","key":"10.1016\/j.jim.2026.114105_bb0015","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/nature11537","article-title":"In vivo genome editing using a high-efficiency TALEN system","volume":"491","author":"Bedell","year":"2012","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0020","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/emboj.2008.257","article-title":"Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites","volume":"28","author":"Borde","year":"2009","journal-title":"EMBO J."},{"issue":"5","key":"10.1016\/j.jim.2026.114105_bb0025","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/S1074-7613(00)00059-5","article-title":"DNA double-strand breaks in immunoglobulin genes undergoing somatic hypermutation","volume":"13","author":"Bross","year":"2000","journal-title":"Immunity"},{"issue":"20","key":"10.1016\/j.jim.2026.114105_bb0030","doi-asserted-by":"crossref","first-page":"12281","DOI":"10.1093\/nar\/gkae867","article-title":"Core enhancers of the 3\u2032RR optimize IgH nuclear position and loop conformation for successful oriented class switch recombination","volume":"52","author":"Bruzeau","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"10.1016\/j.jim.2026.114105_bb0035","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/0022-2836(89)90007-7","article-title":"An amino-terminal fragment of GAL4 binds DNA as a dimer","volume":"209","author":"Carey","year":"1989","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.jim.2026.114105_bb0040","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.cell.2011.07.049","article-title":"Genome-wide translocation sequencing reveals mechanisms of chromosome breaks and rearrangements in B cells","volume":"147","author":"Chiarle","year":"2011","journal-title":"Cell"},{"issue":"8","key":"10.1016\/j.jim.2026.114105_bb0045","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1038\/nmeth.2543","article-title":"TALE-mediated modulation of transcriptional enhancers in vivo","volume":"10","author":"Crocker","year":"2013","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.jim.2026.114105_bb0050","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.bbrc.2013.08.013","article-title":"Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR","volume":"439","author":"Fujita","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0055","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1534\/genetics.115.177287","article-title":"The double-strand break landscape of meiotic chromosomes is shaped by the Paf1 transcription elongation complex in Saccharomyces cerevisiae","volume":"202","author":"Gothwal","year":"2016","journal-title":"Genetics"},{"key":"10.1016\/j.jim.2026.114105_bb0060","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae204","article-title":"BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL","author":"Gothwal","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0065","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.molcel.2009.06.022","article-title":"Initial stages of V(D)J recombination: the organization of RAG1\/2 and RSS DNA in the postcleavage complex","volume":"35","author":"Grundy","year":"2009","journal-title":"Mol. Cell"},{"issue":"15","key":"10.1016\/j.jim.2026.114105_bb0070","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2019102931","article-title":"SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination","volume":"39","author":"Husain","year":"2020","journal-title":"EMBO J."},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0075","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.cell.2008.03.024","article-title":"The 3D structure of the immunoglobulin heavy-chain locus: implications for long-range genomic interactions","volume":"133","author":"Jhunjhunwala","year":"2008","journal-title":"Cell"},{"issue":"15","key":"10.1016\/j.jim.2026.114105_bb0080","doi-asserted-by":"crossref","first-page":"4162","DOI":"10.1093\/emboj\/cdf394","article-title":"Ordered assembly of the V(D)J synaptic complex ensures accurate recombination","volume":"21","author":"Jones","year":"2002","journal-title":"EMBO J."},{"issue":"1","key":"10.1016\/j.jim.2026.114105_bb0085","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/sj.leu.2403534","article-title":"Raji revisited: cytogenetics of the original Burkitt's lymphoma cell line","volume":"19","author":"Karpova","year":"2005","journal-title":"Leukemia"},{"issue":"21","key":"10.1016\/j.jim.2026.114105_bb0090","doi-asserted-by":"crossref","first-page":"3982","DOI":"10.1016\/j.febslet.2014.09.014","article-title":"Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases","volume":"588","author":"Kasparek","year":"2014","journal-title":"FEBS Lett."},{"issue":"3","key":"10.1016\/j.jim.2026.114105_bb0095","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0092-8674(00)81876-0","article-title":"Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family","volume":"88","author":"Keeney","year":"1997","journal-title":"Cell"},{"issue":"4","key":"10.1016\/j.jim.2026.114105_bb0100","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrc1589","article-title":"Mechanisms of B-cell lymphoma pathogenesis","volume":"5","author":"K\u00fcppers","year":"2005","journal-title":"Nat. Rev. Cancer"},{"issue":"6","key":"10.1016\/j.jim.2026.114105_bb0105","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nrc.2016.40","article-title":"Mechanisms of human lymphoid chromosomal translocations","volume":"16","author":"Lieber","year":"2016","journal-title":"Nat. Rev. Cancer"},{"issue":"10","key":"10.1016\/j.jim.2026.114105_bb0110","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1186\/gb-2004-5-10-242","article-title":"Hotspots of homologous recombination in the human genome: not all homologous sequences are equal","volume":"5","author":"Lupski","year":"2004","journal-title":"Genome Biol."},{"issue":"6368","key":"10.1016\/j.jim.2026.114105_bb0115","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/356408a0","article-title":"DNA recognition by GAL4: structure of a protein-DNA complex","volume":"356","author":"Marmorstein","year":"1992","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0120","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nbt.1755","article-title":"A TALE nuclease architecture for efficient genome editing","volume":"29","author":"Miller","year":"2011","journal-title":"Nat. Biotechnol."},{"issue":"5","key":"10.1016\/j.jim.2026.114105_bb0125","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/S0092-8674(00)00078-7","article-title":"Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme","volume":"102","author":"Muramatsu","year":"2000","journal-title":"Cell"},{"issue":"3","key":"10.1016\/j.jim.2026.114105_bb0130","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/s41580-019-0209-0","article-title":"Regulation of long non-coding RNAs and genome dynamics by the RNA surveillance machinery","volume":"21","author":"Nair","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"1","key":"10.1016\/j.jim.2026.114105_bb0135","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0167-4781(92)90134-L","article-title":"Somatic hypermutagenesis in immunoglobulin genes. II. Influence of neighbouring base sequences on mutagenesis","volume":"1171","author":"Rogozin","year":"1992","journal-title":"Biochim. Biophys. Acta"},{"issue":"51","key":"10.1016\/j.jim.2026.114105_bb0140","doi-asserted-by":"crossref","first-page":"22190","DOI":"10.1073\/pnas.1016923108","article-title":"Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination","volume":"107","author":"Stanlie","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"10.1016\/j.jim.2026.114105_bb0145","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.molcel.2014.05.018","article-title":"Chromatin reader Brd4 functions in Ig class switching as a repair complex adaptor of nonhomologous end-joining","volume":"55","author":"Stanlie","year":"2014","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jim.2026.114105_bb0150","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1146\/annurev.immunol.26.021607.090248","article-title":"Mechanism and regulation of class switch recombination","volume":"26","author":"Stavnezer","year":"2008","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.jim.2026.114105_bb0155","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.07424","article-title":"Transcription dynamically patterns the meiotic chromosome-axis interface","volume":"4","author":"Sun","year":"2015","journal-title":"Elife"},{"key":"10.1016\/j.jim.2026.114105_bb0160","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0070-2153(08)60171-4","article-title":"Meiotic recombination hotspots: shaping the genome and insights into hypervariable minisatellite DNA change","volume":"37","author":"Wahls","year":"1998","journal-title":"Curr. Top. Dev. Biol."},{"issue":"5575","key":"10.1016\/j.jim.2026.114105_bb0165","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1126\/science.1071556","article-title":"AID enzyme-induced hypermutation in an actively transcribed gene in fibroblasts","volume":"296","author":"Yoshikawa","year":"2002","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.jim.2026.114105_bb0170","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1002\/gcc.2870090408","article-title":"Breakpoints of Burkitt\u2019s lymphoma t(8;22) translocations map within a distance of 300 kb downstream of MYC","volume":"9","author":"Zeidler","year":"1994","journal-title":"Genes Chromosomes Cancer"},{"issue":"2","key":"10.1016\/j.jim.2026.114105_bb0175","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/nbt.1775","article-title":"Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription","volume":"29","author":"Zhang","year":"2011","journal-title":"Nat. Biotechnol."}],"container-title":["Journal of Immunological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000815?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022175926000815?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:27:26Z","timestamp":1788553646000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022175926000815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":35,"alternative-id":["S0022175926000815"],"URL":"https:\/\/doi.org\/10.1016\/j.jim.2026.114105","relation":{},"ISSN":["0022-1759"],"issn-type":[{"value":"0022-1759","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation of region-specific factors driving antibody class-switch recombination from the immunoglobulin heavy chain locus","name":"articletitle","label":"Article Title"},{"value":"Journal of Immunological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jim.2026.114105","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114105"}}