{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:51:08Z","timestamp":1772121068473,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001506","name":"Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["BIO2011-25343"],"award-info":[{"award-number":["BIO2011-25343"]}],"id":[{"id":"10.13039\/501100001506","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001506","name":"Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["BIO2014-55462-R"],"award-info":[{"award-number":["BIO2014-55462-R"]}],"id":[{"id":"10.13039\/501100001506","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001506","name":"Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["SAF2012-39444-C02"],"award-info":[{"award-number":["SAF2012-39444-C02"]}],"id":[{"id":"10.13039\/501100001506","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondo de Investigaciones Sanitarias de la Seguridad Social","award":["PI11\/00763"],"award-info":[{"award-number":["PI11\/00763"]}]},{"name":"Ministry of Education","award":["FPU13\/02899"],"award-info":[{"award-number":["FPU13\/02899"]}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","journalofinfection.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.jinf.2016.04.010","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T17:28:02Z","timestamp":1462037282000},"page":"71-81","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["DiiA is a novel dimorphic cell wall protein of Streptococcus pneumoniae involved in invasive disease"],"prefix":"10.1016","volume":"73","author":[{"given":"Mar\u00eda S.","family":"Escolano-Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnau","family":"Domenech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Yuste","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda I.","family":"Cercenado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Ardanuy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josefina","family":"Li\u00f1ares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela G.","family":"de la Campa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio J.","family":"Martin-Galiano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jinf.2016.04.010_bib1","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1128\/IAI.00881-09","article-title":"The Streptococcus pneumoniae capsule inhibits complement activity and neutrophil phagocytosis by multiple mechanisms","volume":"78","author":"Hyams","year":"2010","journal-title":"Infect Immun"},{"key":"10.1016\/j.jinf.2016.04.010_bib2","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/1471-2180-10-190","article-title":"New adhesin functions of surface-exposed pneumococcal proteins","volume":"10","author":"Frolet","year":"2010","journal-title":"BMC Microbiol"},{"issue":"11","key":"10.1016\/j.jinf.2016.04.010_bib3","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0966-842X(01)02207-7","article-title":"Genome-based analysis of pneumococcal virulence factors: the quest for novel vaccine antigens and drug targets","volume":"9","author":"Paton","year":"2001","journal-title":"Trends Microbiol"},{"key":"10.1016\/j.jinf.2016.04.010_bib4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1128\/CVI.00647-14","article-title":"Genetic, biochemical, and serological characterization of a new pneumococcal serotype, 6H, and generation of a pneumococcal strain producing three different capsular repeat units","volume":"22","author":"Park","year":"2015","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.jinf.2016.04.010_bib5","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1086\/374624","article-title":"Clonal relationships between invasive and carriage Streptococcus pneumoniae and serotype- and clone-specific differences in invasive disease potential","volume":"187","author":"Brueggemann","year":"2003","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.010_bib6","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/nrmicro1871","article-title":"The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease","volume":"6","author":"Kadioglu","year":"2008","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jinf.2016.04.010_bib7","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0140-6736(09)61204-6","article-title":"Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates","volume":"374","author":"O'Brien","year":"2009","journal-title":"Lancet"},{"key":"10.1016\/j.jinf.2016.04.010_bib8","first-page":"144","article-title":"Invasive pneumococcal disease in children 5 years after conjugate vaccine introduction \u2013 eight states, 1998\u20132005","volume":"57","author":"Centers for Disease Control and Prevention (CDC)","year":"2008","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"10.1016\/j.jinf.2016.04.010_bib9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0966-842X(99)01493-6","article-title":"Pathoadaptive mutations: gene loss and variation in bacterial pathogens","volume":"7","author":"Sokurenko","year":"1999","journal-title":"Trends Microbiol"},{"issue":"5626","key":"10.1016\/j.jinf.2016.04.010_bib10","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1126\/science.1085371","article-title":"Evolution of the protein repertoire","volume":"300","author":"Chothia","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.jinf.2016.04.010_bib11","doi-asserted-by":"crossref","first-page":"4803","DOI":"10.1002\/j.1460-2075.1993.tb06169.x","article-title":"Cell wall sorting signals in surface proteins of gram-positive bacteria","volume":"12","author":"Schneewind","year":"1993","journal-title":"EMBO J"},{"key":"10.1016\/j.jinf.2016.04.010_bib12","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1046\/j.1365-2958.1999.01223.x","article-title":"High-frequency intracellular invasion of epithelial cells by serotype M1 group A streptococci: M1 protein-mediated invasion and cytoskeletal rearrangements","volume":"31","author":"Dombek","year":"1999","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.jinf.2016.04.010_bib13","doi-asserted-by":"crossref","first-page":"24714","DOI":"10.1074\/jbc.M402164200","article-title":"Alpha C protein of group B Streptococcus binds host cell surface glycosaminoglycan and enters cells by an actin-dependent mechanism","volume":"279","author":"Baron","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jinf.2016.04.010_bib14","doi-asserted-by":"crossref","first-page":"5216","DOI":"10.1128\/iai.65.12.5216-5221.1997","article-title":"Immunogenicity and protective efficacy of the alpha C protein of group B streptococci are inversely related to the number of repeats","volume":"65","author":"Gravekamp","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.jinf.2016.04.010_bib15","doi-asserted-by":"crossref","first-page":"D71","DOI":"10.1093\/nar\/gkr981","article-title":"Reorganizing the protein space at the Universal Protein Resource (UniProt)","volume":"40","author":"The Uniprot Consortium","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jinf.2016.04.010_bib16","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/10665270050081478","article-title":"A greedy algorithm for aligning DNA sequences","volume":"7","author":"Zhang","year":"2000","journal-title":"J Comput Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib18","doi-asserted-by":"crossref","first-page":"R22","DOI":"10.1186\/gb-2007-8-2-r22","article-title":"Rapid, accurate, computational discovery of Rho-independent transcription terminators illuminates their relationship to DNA uptake","volume":"8","author":"Kingsford","year":"2007","journal-title":"Genome Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib19","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1093\/nar\/gki232","article-title":"A novel method for accurate operon predictions in all sequenced prokaryotes","volume":"33","author":"Price","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jinf.2016.04.010_bib20","doi-asserted-by":"crossref","first-page":"D290","DOI":"10.1093\/nar\/gkr1065","article-title":"The Pfam protein families database","volume":"40","author":"Punta","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jinf.2016.04.010_bib21","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","article-title":"Protein secondary structure prediction based on position-specific scoring matrices","volume":"292","author":"Jones","year":"1999","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib22","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A combined transmembrane topology and signal peptide prediction method","volume":"338","author":"Kall","year":"2004","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib23","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1016\/j.str.2003.10.002","article-title":"Protein disorder prediction: implications for structural proteomics","volume":"11","author":"Linding","year":"2003","journal-title":"Structure"},{"key":"10.1016\/j.jinf.2016.04.010_bib24","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0097-8485(94)85023-2","article-title":"Non-globular domains in protein sequences: automated segmentation using complexity measures","volume":"18","author":"Wootton","year":"1994","journal-title":"Comput Chem"},{"key":"10.1016\/j.jinf.2016.04.010_bib25","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1126\/science.252.5009.1162","article-title":"Predicting coiled coils from protein sequences","volume":"252","author":"Lupas","year":"1991","journal-title":"Science"},{"key":"10.1016\/j.jinf.2016.04.010_bib26","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/bioinformatics\/bti797","article-title":"Paircoil2: improved prediction of coiled coils from sequence","volume":"22","author":"McDonnell","year":"2006","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jinf.2016.04.010_bib27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/0378-1119(90)90115-8","article-title":"Comparative expression of the pC194 cat gene in Streptococcus pneumoniae, Bacillus subtilis and Escherichia coli","volume":"86","author":"Ballester","year":"1990","journal-title":"Gene"},{"key":"10.1016\/j.jinf.2016.04.010_bib28","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1261\/rna.027359.111","article-title":"Identification of 88 regulatory small RNAs in the TIGR4 strain of the human pathogen Streptococcus pneumoniae","volume":"18","author":"Acebo","year":"2012","journal-title":"RNA"},{"key":"10.1016\/j.jinf.2016.04.010_bib29","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.4049\/jimmunol.175.3.1813","article-title":"Additive inhibition of complement deposition by pneumolysin and PspA facilitates Streptococcus pneumoniae septicemia","volume":"175","author":"Yuste","year":"2005","journal-title":"J Immunol"},{"key":"10.1016\/j.jinf.2016.04.010_bib30","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/S1286-4579(01)01496-4","article-title":"Use of mixed infections with Salmonella strains to study virulence genes and their interactions in\u00a0vivo","volume":"3","author":"Beuzon","year":"2001","journal-title":"Microbes Infect"},{"key":"10.1016\/j.jinf.2016.04.010_bib31","doi-asserted-by":"crossref","first-page":"e23626","DOI":"10.1371\/journal.pone.0023626","article-title":"Nasopharyngeal colonization and invasive disease are enhanced by the cell wall hydrolases LytB and LytC of Streptococcus pneumoniae","volume":"6","author":"Ramos-Sevillano","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.jinf.2016.04.010_bib32","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1471-2105-3-5","article-title":"Re-annotation of genome microbial coding-sequences: finding new genes and inaccurately annotated genes","volume":"3","author":"Bocs","year":"2002","journal-title":"BMC Bioinforma"},{"key":"10.1016\/j.jinf.2016.04.010_bib33","doi-asserted-by":"crossref","first-page":"e45103","DOI":"10.1371\/journal.pone.0045103","article-title":"Predicting statistical properties of open reading frames in bacterial genomes","volume":"7","author":"Mir","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jinf.2016.04.010_bib34","first-page":"1389","article-title":"Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors","volume":"45","author":"Hava","year":"2002","journal-title":"Mol Microbiol"},{"issue":"1","key":"10.1016\/j.jinf.2016.04.010_bib35","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1084\/jem.20071168","article-title":"Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of pneumococcus with human antibodies","volume":"205","author":"Giefing","year":"2008","journal-title":"J Exp Med"},{"key":"10.1016\/j.jinf.2016.04.010_bib36","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.ygeno.2012.08.001","article-title":"Protein domain repetition is enriched in Streptococcal cell-surface proteins","volume":"100","author":"Lin","year":"2012","journal-title":"Genomics"},{"key":"10.1016\/j.jinf.2016.04.010_bib37","doi-asserted-by":"crossref","first-page":"8186","DOI":"10.1128\/JB.00690-07","article-title":"Comparative genomic analyses of seventeen Streptococcus pneumoniae strains: insights into the pneumococcal supragenome","volume":"189","author":"Hiller","year":"2007","journal-title":"J Bacteriol"},{"key":"10.1016\/j.jinf.2016.04.010_bib38","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1128\/CVI.00271-10","article-title":"PsrP, a protective pneumococcal antigen, is highly prevalent in children with pneumonia and is strongly associated with clonal type","volume":"17","author":"Mu\u00f1oz-Almagro","year":"2010","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.jinf.2016.04.010_bib39","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1471-2180-9-80","article-title":"Diversity of pneumococcal surface protein A (PspA) among prevalent clones in Spain","volume":"9","author":"Rolo","year":"2009","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.jinf.2016.04.010_bib40","doi-asserted-by":"crossref","first-page":"e28738","DOI":"10.1371\/journal.pone.0028738","article-title":"Streptococcus pneumoniae in biofilms are unable to cause invasive disease due to altered virulence determinant production","volume":"6","author":"Sanchez","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.jinf.2016.04.010_bib41","first-page":"169","article-title":"Yersinia infection tools-characterization of structure and function of adhesins","volume":"2","author":"Mikula","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.jinf.2016.04.010_bib42","doi-asserted-by":"crossref","first-page":"e42595","DOI":"10.1371\/journal.pone.0042595","article-title":"Invasive pneumococcal disease in healthy adults: increase of empyema associated with the clonal-type Sweden(1)-ST306","volume":"7","author":"Grau","year":"2012","journal-title":"PLoS One"},{"issue":"9","key":"10.1016\/j.jinf.2016.04.010_bib43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1000933","article-title":"Plasmodium falciparum erythrocyte membrane protein 1 diversity in seven genomes\u2013divide and conquer","volume":"6","author":"Rask","year":"2010","journal-title":"PLoS Comput Biol"},{"key":"10.1016\/j.jinf.2016.04.010_bib44","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1128\/iai.64.10.4255-4260.1996","article-title":"A protective surface protein from type V group B streptococci shares N-terminal sequence homology with the alpha C protein","volume":"64","author":"Lachenauer","year":"1996","journal-title":"Infect Immun"},{"issue":"3","key":"10.1016\/j.jinf.2016.04.010_bib45","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.coi.2011.04.002","article-title":"Next generation pneumococcal vaccines","volume":"23","author":"Moffitt","year":"2011","journal-title":"Curr Opin Immunol"}],"container-title":["Journal of Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445316300378?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445316300378?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:23:01Z","timestamp":1759008181000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0163445316300378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["S0163445316300378"],"URL":"https:\/\/doi.org\/10.1016\/j.jinf.2016.04.010","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726307838.793522911","asserted-by":"object"}]},"ISSN":["0163-4453"],"issn-type":[{"value":"0163-4453","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"DiiA is a novel dimorphic cell wall protein of Streptococcus pneumoniae involved in invasive disease","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinf.2016.04.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The British Infection Association. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}