{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:13:44Z","timestamp":1790669624180,"version":"4.1.0"},"reference-count":32,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","journalofinfection.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1016\/j.jinf.2016.04.039","type":"journal-article","created":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T01:37:34Z","timestamp":1466213854000},"page":"123-128","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prospective evaluation of RT-PCR on sputum versus culture, urinary antigens and serology for Legionnaire's disease diagnosis"],"prefix":"10.1016","volume":"73","author":[{"given":"Elisabeth","family":"Botelho-Nevers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Grattard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Viallon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Allegra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jarraud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Verhoeven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Marcuccilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9deric","family":"Lucht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Pozzetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Berthelot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jinf.2016.04.039_bib1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/CMR.15.3.506-526.2002","article-title":"Legionella and Legionnaires' disease: 25 years of investigation","volume":"15","author":"Fields","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.jinf.2016.04.039_bib2","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1056\/NEJM199709043371006","article-title":"Legionellosis","volume":"337","author":"Stout","year":"1997","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jinf.2016.04.039_bib3","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1086\/342903","article-title":"Trends in legionnaires disease, 1980\u20131998: declining mortality and new patterns of diagnosis","volume":"35","author":"Benin","year":"2002","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.039_bib4","doi-asserted-by":"crossref","first-page":"20417","DOI":"10.2807\/ese.18.10.20417-en","article-title":"Legionnaires disease in Europe, 2009\u20132010","volume":"18","author":"Beaute","year":"2013","journal-title":"Euro Surveill"},{"key":"10.1016\/j.jinf.2016.04.039_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinf.2007.09.010","article-title":"Legionella spp. and Legionnaires' disease","volume":"56","author":"Diederen","year":"2008","journal-title":"J Infect"},{"key":"10.1016\/j.jinf.2016.04.039_bib6","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1086\/345529","article-title":"Diagnosis of Legionella infection","volume":"36","author":"Murdoch","year":"2003","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.039_bib7","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1128\/JCM.41.2.838-840.2003","article-title":"Clinical utility of urinary antigen detection for diagnosis of community-acquired, travel-associated, and nosocomial legionnaires' disease","volume":"41","author":"Helbig","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib8","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1093\/cid\/cit504","article-title":"Impact of routine systematic PCR testing on case finding for Legionnaires' disease: a pre-post comparison study","volume":"57","author":"Murdoch","year":"2013","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.039_bib9","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1183\/09031936.00191913","article-title":"Enhanced detection of Legionnaires' disease by PCR testing of induced sputum and throat swabs","volume":"43","author":"Maze","year":"2014","journal-title":"Eur Respir J"},{"key":"10.1016\/j.jinf.2016.04.039_bib10","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1128\/JCM.39.7.2618-2626.2001","article-title":"Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture","volume":"39","author":"Hayden","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib11","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/978-1-62703-161-5_3","article-title":"Sequence-based identification of legionella","volume":"954","author":"Ratcliff","year":"2013","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jinf.2016.04.039_bib12","doi-asserted-by":"crossref","first-page":"4016","DOI":"10.1128\/JCM.41.9.4016-4021.2003","article-title":"Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species","volume":"41","author":"Templeton","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib13","series-title":"Le risque li\u00e9 aux l\u00e9gionelles: Guide d'investigation et d'aide \u00e0 la gestion","author":"Conseil sup\u00e9rieur d'hygi\u00e8ne publique de France","year":"2005"},{"issue":"Suppl.","key":"10.1016\/j.jinf.2016.04.039_bib14","first-page":"25","article-title":"Pneumonia: review of guidelines","volume":"60","author":"Dhar","year":"2012","journal-title":"J Assoc Physicians India"},{"key":"10.1016\/j.jinf.2016.04.039_bib15","series-title":"Legionellose: Comment-signaler-et-notifier-cette-maladie","author":"Institut de veille Sanitaire","year":"2011"},{"key":"10.1016\/j.jinf.2016.04.039_bib16","unstructured":"Technical document. European Legionnaires' Disease Surveillance Network (ELDSNet) Operating procedures. http:\/\/ecdc.europa.eu\/en\/healthtopics\/legionnaires_disease\/ELDSNet\/Documents\/EWGLI-Technical-Guidelines.pdf. [accessed 12.03.16]."},{"key":"10.1016\/j.jinf.2016.04.039_bib17","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1128\/JCM.01808-15","article-title":"Utility of PCR, culture, and antigen detection methods for diagnosis of Legionellosis","volume":"53","author":"Chen","year":"2015","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib18","doi-asserted-by":"crossref","first-page":"3814","DOI":"10.1128\/JCM.40.10.3814-3817.2002","article-title":"Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis","volume":"40","author":"Reischl","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.07.002","article-title":"Development and evaluation of a Taqman duplex real-time PCR quantification method for reliable enumeration of Legionella pneumophila in water samples","volume":"68","author":"Behets","year":"2007","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.jinf.2016.04.039_bib20","series-title":"REMIC","author":"Groupe R\u00e9mic de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie","year":"2010"},{"key":"10.1016\/j.jinf.2016.04.039_bib21","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/S1473-3099(09)70148-3","article-title":"Atypical pneumonia\u2013time to breathe new life into a useful term?","volume":"9","author":"Murdoch","year":"2009","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.039_bib22","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1136\/thoraxjnl-2013-203551","article-title":"Community-acquired pneumonia in Chile: the clinical relevance in the detection of viruses and atypical bacteria","volume":"68","author":"Luchsinger","year":"2013","journal-title":"Thorax"},{"key":"10.1016\/j.jinf.2016.04.039_bib23","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1378\/chest.120.4.1049","article-title":"Can Legionnaires disease be diagnosed by clinical criteria? A critical review","volume":"120","author":"Mulazimoglu","year":"2001","journal-title":"Chest"},{"key":"10.1016\/j.jinf.2016.04.039_bib24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1128\/JCM.01196-07","article-title":"Utility of real-time PCR for diagnosis of Legionnaires' disease in routine clinical practice","volume":"46","author":"Diederen","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib25","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1469-0691.2009.02812.x","article-title":"Quantitative real-time PCR tests for diagnostic and prognostic purposes in cases of legionellosis","volume":"16","author":"Maurin","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jinf.2016.04.039_bib26","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1099\/jmm.0.05464-0","article-title":"Laboratory diagnosis of legionnaires' disease due to Legionella pneumophila serogroup 1: comparison of phenotypic and genotypic methods","volume":"53","author":"Lindsay","year":"2004","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.jinf.2016.04.039_bib27","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1556\/amicr.59.2012.3.6","article-title":"Hospital acquired pneumonia: comparison of culture and real-time PCR assays for detection of Legionella pneumophila from respiratory specimens at Tehran hospitals","volume":"59","author":"Fard","year":"2012","journal-title":"Acta Microbiol Immunol Hung"},{"key":"10.1016\/j.jinf.2016.04.039_bib28","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.mimet.2014.01.001","article-title":"Evaluation of BMPA, MWY, GVPC and BCYE media for the isolation of Legionella species from respiratory samples","volume":"98","author":"Descours","year":"2014","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.jinf.2016.04.039_bib29","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1183\/09031936.00076911","article-title":"Factors associated with hospital mortality in community-acquired legionellosis in France","volume":"39","author":"Chidiac","year":"2012","journal-title":"Eur Respir J"},{"key":"10.1016\/j.jinf.2016.04.039_bib30","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1093\/clinids\/20.5.1286","article-title":"Reevaluation of the definition of Legionnaires' disease: use of the urinary antigen assay. Community Based Pneumonia Incidence Study Group","volume":"20","author":"Plouffe","year":"1995","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jinf.2016.04.039_bib31","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1007\/s15010-006-6639-6","article-title":"Detection of legionella species in clinical samples: comparison of polymerase chain reaction and urinary antigen detection kits","volume":"34","author":"Koide","year":"2006","journal-title":"Infection"},{"key":"10.1016\/j.jinf.2016.04.039_bib32","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1007\/s00134-010-1859-8","article-title":"Septic shock due to Legionella pneumophila serogroup 2: usefulness of molecular biology for diagnosis, treatment and epidemiological investigation","volume":"36","author":"Grattard","year":"2010","journal-title":"Intensive Care Med"}],"container-title":["Journal of Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445316301281?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445316301281?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:23:04Z","timestamp":1759008184000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0163445316301281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["S0163445316301281"],"URL":"https:\/\/doi.org\/10.1016\/j.jinf.2016.04.039","relation":{},"ISSN":["0163-4453"],"issn-type":[{"value":"0163-4453","type":"print"}],"subject":[],"published":{"date-parts":[[2016,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Prospective evaluation of RT-PCR on sputum versus culture, urinary antigens and serology for Legionnaire's disease diagnosis","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinf.2016.04.039","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The British Infection Association. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}