{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:04:11Z","timestamp":1792037051681,"version":"4.4.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T00:00:00Z","timestamp":1713571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Zhejiang Province Natural Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","clinicalkey.com","journalofinfection.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.jinf.2024.106166","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T12:26:00Z","timestamp":1713961560000},"page":"106166","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["Molecular rapid diagnostic testing for bloodstream infections: Nanopore targeted sequencing with pathogen-specific primers"],"prefix":"10.1016","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1892-8603","authenticated-orcid":false,"given":"Dongsheng","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dairong","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Lou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufa","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib1","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftac027","article-title":"Burden of bacterial bloodstream infections and recent advances for diagnosis","volume":"80","author":"Costa","year":"2022","journal-title":"Pathog Dis"},{"key":"10.1016\/j.jinf.2024.106166_bib2","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3389\/fcimb.2019.00148","article-title":"The healthy human blood microbiome: fact or fiction?","volume":"9","author":"Castillo","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"10.1016\/j.jinf.2024.106166_bib3","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1099\/jmm.0.001209","article-title":"Improving the recovery and detection of bloodstream pathogens from blood culture","volume":"69","author":"Falconer","year":"2020","journal-title":"J Med Microbiol"},{"issue":"2","key":"10.1016\/j.jinf.2024.106166_bib4","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.cmi.2019.11.017","article-title":"Bloodstream infections \u2013 standard and progress in pathogen diagnostics","volume":"26","author":"Lamy","year":"2020","journal-title":"Clin Microbiol Infect"},{"issue":"4","key":"10.1016\/j.jinf.2024.106166_bib5","doi-asserted-by":"crossref","first-page":"4","DOI":"10.3390\/antibiotics11040511","article-title":"Diagnosis of bloodstream infections: an evolution of technologies towards accurate and rapid identification and antibiotic susceptibility testing","volume":"11","author":"Tjandra","year":"2022","journal-title":"Antibiotics"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib6","doi-asserted-by":"crossref","DOI":"10.1186\/s13054-022-04116-8","article-title":"Clinical validation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections in ICU practice: a promising diagnostic tool","volume":"26","author":"Wu","year":"2022","journal-title":"Crit Care"},{"issue":"3","key":"10.1016\/j.jinf.2024.106166_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00095-17","article-title":"The cost-effectiveness of rapid diagnostic testing for the diagnosis of bloodstream infections with or without antimicrobial stewardship","volume":"31","author":"Pliakos","year":"2018","journal-title":"Clin Microbiol Rev"},{"issue":"11","key":"10.1016\/j.jinf.2024.106166_bib8","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1007\/s10096-015-2466-y","article-title":"Rapid molecular diagnostic tests in patients with bacteremia: evaluation of their impact on decision making and clinical outcomes","volume":"34","author":"Vardakas","year":"2015","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/s41591-020-1105-z","article-title":"Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids","volume":"27","author":"Gu","year":"2021","journal-title":"Nat Med"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib10","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01315-19","article-title":"Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing","volume":"58","author":"Petersen","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.jinf.2024.106166_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00270-22","article-title":"Clinical performance of nanopore targeted sequencing for diagnosing infectious diseases","volume":"10","author":"Fu","year":"2022","journal-title":"Microbiol Spectr"},{"issue":"32","key":"10.1016\/j.jinf.2024.106166_bib12","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202104078","article-title":"Nanopore targeted sequencing for the accurate and comprehensive detection of SARS-CoV-2 and other respiratory viruses","volume":"17","author":"Wang","year":"2021","journal-title":"Small"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib13","first-page":"bpaa26","article-title":"Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples","volume":"6","author":"D'Andreano","year":"2021","journal-title":"Biol Methods Protoc"},{"issue":"6","key":"10.1016\/j.jinf.2024.106166_bib14","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1007\/s40291-023-00669-8","article-title":"Application of nanopore sequencing in the diagnosis and treatment of pulmonary infections","volume":"27","author":"Chen","year":"2023","journal-title":"Mol Diagn Ther"},{"key":"10.1016\/j.jinf.2024.106166_bib15","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03983-22","article-title":"The real-world clinical impact of plasma mNGS testing: an observational study","author":"Han","year":"2023","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.jinf.2024.106166_bib16","article-title":"Applicability of bronchoalveolar lavage fluid and plasma mNGS assays in the diagnosis of pneumonia","author":"Han","year":"2023","journal-title":"Open Forum Infect Dis"},{"issue":"12","key":"10.1016\/j.jinf.2024.106166_bib17","doi-asserted-by":"crossref","first-page":"845","DOI":"10.7326\/M18-2772","article-title":"Performance of the T2Bacteria panel for diagnosing bloodstream infections: a diagnostic accuracy study","volume":"170","author":"Nguyen","year":"2019","journal-title":"Ann Intern Med"},{"issue":"7","key":"10.1016\/j.jinf.2024.106166_bib18","doi-asserted-by":"crossref","first-page":"e419","DOI":"10.1128\/JCM.00419-20","article-title":"Assessment of the clinical utility of plasma metagenomic next-generation sequencing in a pediatric hospital population","volume":"58","author":"Lee","year":"2020","journal-title":"J Clin Microbiol"},{"issue":"8","key":"10.1016\/j.jinf.2024.106166_bib19","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (Sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"key":"10.1016\/j.jinf.2024.106166_bib20","unstructured":"CLSI M100-ED30:2020 performance standards for antimicrobial susceptibility testing, 30th ed."},{"issue":"D1","key":"10.1016\/j.jinf.2024.106166_bib21","doi-asserted-by":"crossref","first-page":"D690","DOI":"10.1093\/nar\/gkac920","article-title":"CARD 2023: expanded curation, support for machine learning, and resistome prediction at the comprehensive antibiotic resistance database","volume":"51","author":"Alcock","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"10.1016\/j.jinf.2024.106166_bib22","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1093\/clinchem\/hvab061","article-title":"Clinical evaluation of an improved metagenomic next-generation sequencing test for the diagnosis of bloodstream infections","volume":"67","author":"Jing","year":"2021","journal-title":"Clin Chem"},{"issue":"4","key":"10.1016\/j.jinf.2024.106166_bib23","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41564-018-0349-6","article-title":"Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease","volume":"4","author":"Blauwkamp","year":"2019","journal-title":"Nat Microbiol"},{"issue":"6","key":"10.1016\/j.jinf.2024.106166_bib24","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/j.chest.2016.08.1460","article-title":"Culture-negative severe sepsis: nationwide trends and outcomes","volume":"150","author":"Gupta","year":"2016","journal-title":"Chest"},{"issue":"12","key":"10.1016\/j.jinf.2024.106166_bib25","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01729-20","article-title":"Retrospective review of clinical utility of shotgun metagenomic sequencing testing of cerebrospinal fluid from a U.S. tertiary care medical center","volume":"58","author":"Rodino","year":"2020","journal-title":"J Clin Microbiol"},{"issue":"7","key":"10.1016\/j.jinf.2024.106166_bib26","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/s41587-019-0156-5","article-title":"Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection","volume":"37","author":"Charalampous","year":"2019","journal-title":"Nat Biotechnol"},{"issue":"5","key":"10.1016\/j.jinf.2024.106166_bib27","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01779-22","article-title":"Background filtering of clinical metagenomic sequencing with a library concentration-normalized model","volume":"10","author":"Du","year":"2022","journal-title":"Microbiol Spectr"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib28","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev-pathmechdis-012418-012751","article-title":"Clinical metagenomic next-generation sequencing for pathogen detection","volume":"14","author":"Gu","year":"2019","journal-title":"Annu Rev Pathol Mech Dis"},{"issue":"12","key":"10.1016\/j.jinf.2024.106166_bib29","doi-asserted-by":"crossref","first-page":"5501","DOI":"10.7150\/thno.45554","article-title":"Liquid biopsy for infectious diseases: a focus on microbial cell-free DNA sequencing","volume":"10","author":"Han","year":"2020","journal-title":"Theranostics"},{"issue":"9","key":"10.1016\/j.jinf.2024.106166_bib30","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.cmi.2018.05.007","article-title":"Diagnosis of bloodstream infections from positive blood cultures and directly from blood samples: recent developments in molecular approaches","volume":"24","author":"Peker","year":"2018","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jinf.2024.106166_bib31","doi-asserted-by":"crossref","DOI":"10.2147\/IDR.S424061","article-title":"Secondary infection surveillance with metagenomic next-generation sequencing in COVID-19 patients: a cross-sectional study","author":"Chen","year":"2023","journal-title":"Infect Drug Resist"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib32","doi-asserted-by":"crossref","DOI":"10.1186\/s13073-022-01072-4","article-title":"Metagenomic prediction of antimicrobial resistance in critically ill patients with lower respiratory tract infections","volume":"14","author":"Serpa","year":"2022","journal-title":"Genome Med"},{"issue":"5","key":"10.1016\/j.jinf.2024.106166_bib33","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.01805-22","article-title":"Novel clinical mNGS-based machine learning model for rapid antimicrobial susceptibility testing of Acinetobacter baumannii","volume":"61","author":"Hu","year":"2023","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10.1016\/j.jinf.2024.106166_bib34","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/s41564-019-0656-6","article-title":"Rapid inference of antibiotic resistance and susceptibility by genomic neighbour typing","volume":"5","author":"B\u0159inda","year":"2020","journal-title":"Nat Microbiol"},{"key":"10.1016\/j.jinf.2024.106166_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/s41576-019-0108-4","article-title":"Sequencing-based methods and resources to study antimicrobial resistance","author":"Boolchandani","year":"2019","journal-title":"Nat Rev Genet"},{"issue":"3","key":"10.1016\/j.jinf.2024.106166_bib36","doi-asserted-by":"crossref","DOI":"10.1128\/msystems.00022-22","article-title":"Performance characteristics of next-generation sequencing for the detection of antimicrobial resistance determinants in Escherichia coli genomes and metagenomes","volume":"7","author":"Rooney","year":"2022","journal-title":"mSystems"},{"key":"10.1016\/j.jinf.2024.106166_bib37","first-page":"1","article-title":"mNGS in clinical microbiology laboratories: on the road to maturity","author":"Han","year":"2019","journal-title":"Crit Rev Microbiol"},{"issue":"1","key":"10.1016\/j.jinf.2024.106166_bib38","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-41903-x","article-title":"Genomic analysis and antimicrobial activity of \u03b2-lactam\/\u03b2-lactamase inhibitors and other agents against KPC-producing Klebsiella pneumoniae clinical isolates from Brazilian hospitals","volume":"13","author":"Camargo","year":"2023","journal-title":"Sci Rep"}],"container-title":["Journal of Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445324001002?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445324001002?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T10:17:50Z","timestamp":1741601870000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0163445324001002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S0163445324001002"],"URL":"https:\/\/doi.org\/10.1016\/j.jinf.2024.106166","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3840855\/v1","asserted-by":"object"}]},"ISSN":["0163-4453"],"issn-type":[{"value":"0163-4453","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular rapid diagnostic testing for bloodstream infections: Nanopore targeted sequencing with pathogen-specific primers","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinf.2024.106166","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Ltd on behalf of The British Infection Association.","name":"copyright","label":"Copyright"}],"article-number":"106166"}}