{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:48:43Z","timestamp":1780408123782,"version":"3.54.1"},"reference-count":48,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011345","name":"Academy of Military Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011345","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015976","name":"State Key Laboratory of Pathogen and Biosecurity","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015976","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2605101"],"award-info":[{"award-number":["2023YFC2605101"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","journalofinfection.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.jinf.2025.106650","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T08:01:29Z","timestamp":1762416089000},"page":"106650","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["High-efficiency inhibition of human adenovirus type 55 replication by CRISPR-Cas12a"],"prefix":"10.1016","volume":"91","author":[{"given":"Lifang","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengwei","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuofan","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1908-2926","authenticated-orcid":false,"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yansong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.immuni.2009.04.015","article-title":"Virus binding to a plasma membrane receptor triggers interleukin-1 alpha-mediated proinflammatory macrophage response in vivo","volume":"31","author":"Di Paolo","year":"2009","journal-title":"Immunity"},{"issue":"4","key":"10.1016\/j.jinf.2025.106650_bib2","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1128\/CMR.00052-07","article-title":"Adenoviruses in immunocompromised hosts","volume":"21","author":"Echavarr\u00eda","year":"2008","journal-title":"Clin Microbiol Rev"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1128\/CMR.00116-13","article-title":"Adenovirus infections in immunocompetent and immunocompromised patients","volume":"27","author":"Lion","year":"2014","journal-title":"Clin Microbiol Rev"},{"issue":"12","key":"10.1016\/j.jinf.2025.106650_bib4","doi-asserted-by":"crossref","first-page":"6111","DOI":"10.1002\/jmv.28074","article-title":"An outbreak of acute respiratory disease caused by HAdV-55 in Beijing, China, 2020","volume":"94","author":"Liu","year":"2022","journal-title":"J Med Virol"},{"key":"10.1016\/j.jinf.2025.106650_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.606195","article-title":"Epidemiology and genetic variabilities of human adenovirus type 55 reveal relative genome stability across time and geographic space in China","volume":"11","author":"Chen","year":"2020","journal-title":"Front Microbiol"},{"issue":"7","key":"10.1016\/j.jinf.2025.106650_bib6","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.3201\/eid2607.191707","article-title":"Human adenovirus type 55 distribution, regional persistence, and genetic variability","volume":"26","author":"Hang","year":"2020","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib7","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1128\/JCM.01694-09","article-title":"Computational analysis identifies human adenovirus type 55 as a re-emergent acute respiratory disease pathogen","volume":"48","author":"Walsh","year":"2010","journal-title":"J Clin Microbiol"},{"issue":"4","key":"10.1016\/j.jinf.2025.106650_bib8","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.jcv.2012.04.009","article-title":"Adenovirus infections in Bordeaux University Hospital 2008-2010: clinical and virological features","volume":"54","author":"Berciaud","year":"2012","journal-title":"J Clin Virol"},{"issue":"9","key":"10.1016\/j.jinf.2025.106650_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00836-22","article-title":"Adenovirus infections: new insights for the clinical laboratory","volume":"62","author":"Kajon","year":"2024","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.jinf.2025.106650_bib10","first-page":"183","article-title":"Research progress in the treatment of human adenovirus infection","volume":"21","author":"Zhang","year":"2024","journal-title":"China Med Her"},{"issue":"9","key":"10.1016\/j.jinf.2025.106650_bib11","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.3201\/eid2509.181937","article-title":"Household transmission of human adenovirus type 55 in case of fatal acute respiratory disease","volume":"25","author":"Jing","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib12","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12879-018-2962-7","article-title":"Case report: a fatal case of disseminated adenovirus infection in a non-transplant adult haematology patient","volume":"18","author":"Joffe","year":"2018","journal-title":"BMC Infect Dis"},{"issue":"9","key":"10.1016\/j.jinf.2025.106650_bib13","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.3390\/microorganisms8091284","article-title":"Recent advances in novel antiviral therapies against human adenovirus","volume":"8","author":"Saha","year":"2020","journal-title":"Microorganisms"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib14","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jcv.2012.08.021","article-title":"Clinical features of radiologically confirmed pneumonia due to adenovirus in children","volume":"56","author":"Chen","year":"2013","journal-title":"J Clin Virol"},{"issue":"4","key":"10.1016\/j.jinf.2025.106650_bib15","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.2189","article-title":"Epidemiology of human adenovirus associated with respiratory infection in southern Brazil","volume":"31","author":"Pscheidt","year":"2021","journal-title":"Rev Med Virol"},{"issue":"5","key":"10.1016\/j.jinf.2025.106650_bib16","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cmi.2019.10.012","article-title":"Early cidofovir administration might be associated with a lower probability of respiratory failure in treating human adenovirus pneumonia: a retrospective cohort study","volume":"26","author":"Ko","year":"2020","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib17","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s13073-017-0502-5","article-title":"Genetic variation in human drug-related genes","volume":"9","author":"Sch\u00e4rfe","year":"2017","journal-title":"Genome Med"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib18","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/mt.2016.10","article-title":"Genome-editing technologies for gene and cell therapy","volume":"24","author":"Maeder","year":"2016","journal-title":"Mol Ther"},{"issue":"08","key":"10.1016\/j.jinf.2025.106650_bib19","first-page":"561","article-title":"Progress and prospects in the development of new nucleic acid drugs and drugs targeting nucleic acid","volume":"48","author":"Gubu","year":"2024","journal-title":"Prog Pharm Sci"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib20","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/s41392-024-02035-4","article-title":"Nucleic acid drugs: recent progress and future perspectives","volume":"9","author":"Sun","year":"2024","journal-title":"Signal Transduct Target Ther"},{"issue":"04","key":"10.1016\/j.jinf.2025.106650_bib21","first-page":"393","article-title":"Application of the CRISPR\/Cas system in antivirus research","volume":"35","author":"Nan-Nan","year":"2019","journal-title":"Chin J Biochem Mol Biol"},{"issue":"7","key":"10.1016\/j.jinf.2025.106650_bib22","doi-asserted-by":"crossref","first-page":"4008","DOI":"10.1093\/nar\/gkab148","article-title":"Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity","volume":"49","author":"Hu","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10.1016\/j.jinf.2025.106650_bib23","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.molcel.2014.03.011","article-title":"CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity","volume":"54","author":"Barrangou","year":"2014","journal-title":"Mol Cell"},{"issue":"2","key":"10.1016\/j.jinf.2025.106650_bib24","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02888-21","article-title":"CRISPR-Cas9 targeting of hepatitis B virus covalently closed circular DNA generates transcriptionally active episomal variants","volume":"13","author":"Martinez","year":"2022","journal-title":"mBio"},{"issue":"4","key":"10.1016\/j.jinf.2025.106650_bib25","doi-asserted-by":"crossref","first-page":"591","DOI":"10.15171\/apb.2018.067","article-title":"The impact of CRISPR-Cas system on antiviral therapy","volume":"8","author":"Bayat","year":"2018","journal-title":"Adv Pharm Bull"},{"issue":"4","key":"10.1016\/j.jinf.2025.106650_bib26","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.cell.2020.04.020","article-title":"Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza","volume":"181","author":"Abbott","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.jinf.2025.106650_bib27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.omtm.2022.02.010","article-title":"A combinatorial CRISPR-Cas12a attack on HIV DNA","volume":"25","author":"Fan","year":"2022","journal-title":"Mol Ther Methods Clin Dev"},{"issue":"10","key":"10.1016\/j.jinf.2025.106650_bib28","doi-asserted-by":"crossref","first-page":"5527","DOI":"10.1093\/nar\/gkaa226","article-title":"Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA","volume":"48","author":"Gao","year":"2020","journal-title":"Nucleic Acids Res"},{"issue":"37","key":"10.1016\/j.jinf.2025.106650_bib29","doi-asserted-by":"crossref","first-page":"22890","DOI":"10.1073\/pnas.2004655117","article-title":"Multiplexed conditional genome editing with Cas12a in Drosophila","volume":"117","author":"Port","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jinf.2025.106650_bib30","article-title":"A rapid and efficient platform for antiviral crRNA screening using CRISPR-Cas13a-based nucleic acid detection","volume":"14","author":"Yang","year":"2023","journal-title":"Front Immunol"},{"issue":"9","key":"10.1016\/j.jinf.2025.106650_bib31","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.molp.2017.06.004","article-title":"CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing","volume":"10","author":"Xie","year":"2017","journal-title":"Mol Plant"},{"issue":"5","key":"10.1016\/j.jinf.2025.106650_bib32","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.3390\/v15051192","article-title":"Generation and characterization of a replication-competent human adenovirus type 55 encoding EGFP","volume":"15","author":"Li","year":"2023","journal-title":"Viruses"},{"issue":"22","key":"10.1016\/j.jinf.2025.106650_bib33","doi-asserted-by":"crossref","first-page":"6994","DOI":"10.3390\/molecules26226994","article-title":"Antiviral therapeutic potential of curcumin: an update","volume":"26","author":"Ardebili","year":"2021","journal-title":"Molecules"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib34","doi-asserted-by":"crossref","first-page":"276","DOI":"10.11613\/BM.2012.031","article-title":"Interrater reliability: the kappa statistic","volume":"22","author":"McHugh","year":"2012","journal-title":"Biochem Med"},{"issue":"6","key":"10.1016\/j.jinf.2025.106650_bib35","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","article-title":"Development and applications of CRISPR-Cas9 for genome engineering","volume":"157","author":"Hsu","year":"2014","journal-title":"Cell"},{"issue":"6387","key":"10.1016\/j.jinf.2025.106650_bib36","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"6213","key":"10.1016\/j.jinf.2025.106650_bib37","doi-asserted-by":"crossref","DOI":"10.1126\/science.1258096","article-title":"Genome editing. The new frontier of genome engineering with CRISPR-Cas9","volume":"346","author":"Doudna","year":"2014","journal-title":"Science"},{"issue":"52","key":"10.1016\/j.jinf.2025.106650_bib38","doi-asserted-by":"crossref","first-page":"7673","DOI":"10.1038\/sj.onc.1209040","article-title":"Recent lessons in gene expression, cell cycle control, and cell biology from adenovirus","volume":"24","author":"Berk","year":"2005","journal-title":"Oncogene"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib39","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a013003","article-title":"Adenovirus DNA replication","volume":"5","author":"Hoeben","year":"2013","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"3","key":"10.1016\/j.jinf.2025.106650_bib40","doi-asserted-by":"crossref","first-page":"388","DOI":"10.3390\/v13030388","article-title":"Adenovirus core proteins: structure and function","volume":"13","author":"Kulanayake","year":"2021","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib41","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1128\/jvi.62.1.266-276.1988","article-title":"Nucleotide sequence and genome organization of canine parvovirus","volume":"62","author":"Reed","year":"1988","journal-title":"J Virol"},{"issue":"6","key":"10.1016\/j.jinf.2025.106650_bib42","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1039\/D0CS01118E","article-title":"Antiviral drug discovery: preparing for the next pandemic","volume":"50","author":"Adamson","year":"2021","journal-title":"Chem Soc Rev"},{"issue":"1","key":"10.1016\/j.jinf.2025.106650_bib43","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0166-3542(02)00098-0","article-title":"Sequence changes in the human adenovirus type 5 DNA polymerase associated with resistance to the broad spectrum antiviral cidofovir","volume":"56","author":"Kinchington","year":"2002","journal-title":"Antivir Res"},{"key":"10.1016\/j.jinf.2025.106650_bib44","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1146\/annurev.me.42.020191.001335","article-title":"Resistance of viruses to antiviral drugs","volume":"42","author":"Freifeld","year":"1991","journal-title":"Annu Rev Med"},{"key":"10.1016\/j.jinf.2025.106650_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2024.101053","article-title":"Target-based drug design strategies to overcome resistance to antiviral agents: opportunities and challenges","volume":"73","author":"Du","year":"2024","journal-title":"Drug Resist Updates"},{"issue":"2","key":"10.1016\/j.jinf.2025.106650_bib46","doi-asserted-by":"crossref","first-page":"215","DOI":"10.2174\/156652306776359478","article-title":"Current strategies and future directions for eluding adenoviral vector immunity","volume":"6","author":"Bangari","year":"2006","journal-title":"Curr Gene Ther"},{"issue":"12","key":"10.1016\/j.jinf.2025.106650_bib47","doi-asserted-by":"crossref","first-page":"7478","DOI":"10.1128\/JVI.79.12.7478-7491.2005","article-title":"Adenovirus binding to blood factors results in liver cell infection and hepatotoxicity","volume":"79","author":"Shayakhmetov","year":"2005","journal-title":"J Virol"},{"issue":"12","key":"10.1016\/j.jinf.2025.106650_bib48","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.3390\/biology10121253","article-title":"Animal models in human adenovirus research","volume":"10","author":"Bertzbach","year":"2021","journal-title":"Biology"}],"container-title":["Journal of Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445325002506?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0163445325002506?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:15:49Z","timestamp":1773879349000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0163445325002506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S0163445325002506"],"URL":"https:\/\/doi.org\/10.1016\/j.jinf.2025.106650","relation":{},"ISSN":["0163-4453"],"issn-type":[{"value":"0163-4453","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-efficiency inhibition of human adenovirus type 55 replication by CRISPR-Cas12a","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinf.2025.106650","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd on behalf of British Infection Association.","name":"copyright","label":"Copyright"}],"article-number":"106650"}}