{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T01:04:50Z","timestamp":1785287090028,"version":"3.55.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100010665","name":"Horizon 2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["101034407"],"award-info":[{"award-number":["101034407"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-22-44CE-0012"],"award-info":[{"award-number":["ANR-22-44CE-0012"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019125","name":"Sorbonne Universit\u00e9","doi-asserted-by":"publisher","award":["ANR-11-LABX-0011-01"],"award-info":[{"award-number":["ANR-11-LABX-0011-01"]}],"id":[{"id":"10.13039\/501100019125","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Inorganic Biochemistry"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.jinorgbio.2025.113064","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T23:49:33Z","timestamp":1757375373000},"page":"113064","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["The thiolation of U34 at carbon 2 in tRNA by Escherichia coli MnmA precedes modification at carbon 5 and is dependent on a [4Fe-4S] cluster"],"prefix":"10.1016","volume":"274","author":[{"given":"Sylvain","family":"Gervason","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sambuddha","family":"Sen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Podskoczyj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grazyna","family":"Leszczynska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Caillat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Ravanat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Fontecave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Golinelli-Pimpaneau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jinorgbio.2025.113064_bb0005","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.tim.2020.06.010","article-title":"Functions of bacterial tRNA modifications: from ubiquity to diversity","volume":"29","author":"de Cr\u00e9cy-Lagard","year":"2021","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107488","article-title":"RNA modifying enzymes shape tRNA biogenesis and function","volume":"300","author":"Schultz","year":"2024","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0015","article-title":"tRNA wobble modifications and protein homeostasis","volume":"4","author":"Ranjan","year":"2016","journal-title":"Translation (Austin)"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0020","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1080\/15476286.2017.1295204","article-title":"Wobble uridine modifications-a reason to live, a reason to die?!","volume":"14","author":"Schaffrath","year":"2017","journal-title":"RNA Biol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0025","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00294-019-01045-y","article-title":"tRNA wobble-uridine modifications as amino acid sensors and regulators of cellular metabolic state","volume":"66","author":"Gupta","year":"2020","journal-title":"Curr. Genet."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0030","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2312874121","article-title":"tRNA epitranscriptome determines pathogenicity of the opportunistic pathogen Pseudomonas aeruginosa","volume":"121","author":"Krueger","year":"2024","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0035","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/s41580-021-00342-0","article-title":"The expanding world of tRNA modifications and their disease relevance","volume":"22","author":"Suzuki","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0040","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/s41576-022-00501-9","article-title":"tRNA dysregulation and disease","volume":"23","author":"Orellana","year":"2022","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0045","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3390\/biom7010027","article-title":"Biosynthesis of sulfur-containing tRNA modifications: a comparison of bacterial, archaeal, and eukaryotic pathways","volume":"7","author":"Cavuzic","year":"2017","journal-title":"Biomolecules"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0050","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222111937","article-title":"Biosynthesis and degradation of sulfur modifications in tRNAs","volume":"22","author":"Shigi","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0055","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.sbi.2020.06.002","article-title":"Structure-based mechanistic insights into catalysis by tRNA thiolation enzymes","volume":"65","author":"Bimai","year":"2020","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0060","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/nature04896","article-title":"Snapshots of tRNA sulphuration via an adenylated intermediate","volume":"442","author":"Numata","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0065","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1093\/nar\/gkn1023","article-title":"Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions","volume":"37","author":"Noma","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0070","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1128\/JB.02625-14","article-title":"Abbreviated pathway for biosynthesis of 2-Thiouridine in Bacillus subtilis","volume":"197","author":"Black","year":"2015","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0075","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1261\/rna.072066.119","article-title":"An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons","volume":"26","author":"Shigi","year":"2020","journal-title":"RNA"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0080","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.1093\/nar\/gkab138","article-title":"Iron sulfur biology invades tRNA modification: the case of U34 sulfuration","volume":"49","author":"Zhou","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0085","doi-asserted-by":"crossref","first-page":"5351","DOI":"10.1038\/s41598-023-32423-9","article-title":"The thiolation of uridine 34 in tRNA, which controls protein translation, depends on a [4Fe-4S]-cluster in Methanococcus maripaludis","volume":"13","author":"Bimai","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0090","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.biochi.2012.02.019","article-title":"Enzymology of tRNA modification in the bacterial MnmEG pathway","volume":"94","author":"Armengod","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0095","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1093\/nar\/gkt1228","article-title":"The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species","volume":"42","author":"Moukadiri","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0100","doi-asserted-by":"crossref","first-page":"26947","DOI":"10.1021\/jacs.3c10182","article-title":"Insights into the mechanism of installation of 5-Carboxymethylaminomethyl uridine Hypermodification by tRNA-modifying enzymes MnmE and MnmG","volume":"145","author":"Bommisetti","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0105","doi-asserted-by":"crossref","first-page":"4094","DOI":"10.1038\/s41467-024-48251-y","article-title":"Cryo-EM structures of the human Elongator complex at work","volume":"15","author":"Abbassi","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0110","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1261\/rna.558707","article-title":"A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast","volume":"13","author":"Bj\u00f6rk","year":"2007","journal-title":"RNA"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175092","article-title":"An unmodified wobble uridine in tRNAs specific for glutamine, lysine, and glutamic acid from Salmonella enterica Serovar typhimurium results in nonviability-due to increased missense errors?","volume":"12","author":"Nilsson","year":"2017","journal-title":"PloS One"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0120","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.4161\/15476286.2014.992269","article-title":"Modification of the wobble uridine in bacterial and mitochondrial tRNAs reading NNA\/NNG triplets of 2-codon boxes","volume":"12","author":"Armengod","year":"2014","journal-title":"RNA Biol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0125","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1128\/JB.02180-12","article-title":"The tRNA thiolation pathway modulates the intracellular redox state in Escherichia coli","volume":"195","author":"Nakayashiki","year":"2013","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0130","article-title":"[4Fe-4S]-dependent enzymes in non-redox tRNA thiolation BBA Mol","volume":"2024","author":"Gervason","year":"1871","journal-title":"Cell Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0135","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1021\/bi026536+","article-title":"MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli","volume":"42","author":"Kambampati","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0140","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1074\/jbc.M409306200","article-title":"Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases","volume":"280","author":"Umeda","year":"2005","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0145","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3390\/inorganics12030067","article-title":"E. coli MnmA is an Fe-S cluster-independent 2-Thiouridylase","volume":"12","author":"Ogunkola","year":"2024","journal-title":"Inorganics"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0150","article-title":"2-Thiouridine formation in Escherichia coli: a critical review","author":"Leimk\u00fchler","year":"2024","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0155","doi-asserted-by":"crossref","first-page":"33588","DOI":"10.1074\/jbc.M104067200","article-title":"The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA","volume":"276","author":"Mueller","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0160","doi-asserted-by":"crossref","first-page":"16068","DOI":"10.1016\/S0021-9258(18)48580-8","article-title":"Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase","volume":"271","author":"Flint","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0165","series-title":"Revision (Accounts Chem Res)","article-title":"Dissecting the mechanism of biosynthesis of sulfurated biomolecules: The case of tRNA sulfuration","author":"Gervason","year":"2025"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0170","doi-asserted-by":"crossref","first-page":"23022","DOI":"10.1074\/jbc.M401757200","article-title":"Substrate specificity for 4-thiouridine modification in Escherichia coli","volume":"279","author":"Lauhon","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0175","doi-asserted-by":"crossref","first-page":"6820","DOI":"10.1128\/JB.184.24.6820-6829.2002","article-title":"Requirement for IscS in biosynthesis of all thionucleosides in Escherichia coli","volume":"184","author":"Lauhon","year":"2002","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0180","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.molcel.2005.11.001","article-title":"Mechanistic insights into multiple sulfur mediators sulfur relay by involved in thiouridine biosynthesis at tRNA wobble positions","volume":"21","author":"Ikeuchi","year":"2006","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0185","doi-asserted-by":"crossref","first-page":"7960","DOI":"10.1093\/nar\/gku508","article-title":"TtcA a new tRNA-thioltransferase with an Fe-S cluster","volume":"42","author":"Bouvier","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0190","doi-asserted-by":"crossref","first-page":"12969","DOI":"10.1093\/nar\/gkac1156","article-title":"A subclass of archaeal U8-tRNA sulfurases requires a [4Fe-4S] cluster for catalysis","volume":"50","author":"He","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0195","doi-asserted-by":"crossref","first-page":"7355","DOI":"10.1073\/pnas.1700902114","article-title":"Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster","volume":"114","author":"Arragain","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0200","doi-asserted-by":"crossref","first-page":"13264","DOI":"10.1074\/jbc.273.21.13264","article-title":"Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii","volume":"273","author":"Zheng","year":"1998","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0205","doi-asserted-by":"crossref","first-page":"4954","DOI":"10.1073\/pnas.1615585114","article-title":"Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA","volume":"114","author":"Chen","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0210","doi-asserted-by":"crossref","first-page":"12220","DOI":"10.1021\/bi049143e","article-title":"Mechanism of cysteine desulfurase Slr0387 from Synechocystis sp. PCC 6803: kinetic analysis of cleavage of the persulfide intermediate by chemical reductants","volume":"43","author":"Behshad","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0215","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.1093\/nar\/12.8.3521","article-title":"Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine","volume":"12","author":"Elseviers","year":"1984","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0220","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1128\/jb.161.1.368-376.1985","article-title":"Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine","volume":"161","author":"Sullivan","year":"1985","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0225","doi-asserted-by":"crossref","first-page":"19551","DOI":"10.1074\/jbc.M401261200","article-title":"Substitutions in an active site loop of Escherichia coli IscS result in specific defects in Fe-S cluster and thionucleoside biosynthesis in vivo","volume":"279","author":"Lauhon","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0230","doi-asserted-by":"crossref","first-page":"3888","DOI":"10.1016\/j.jmb.2013.05.018","article-title":"Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position","volume":"425","author":"Rodriguez-Hernandez","year":"2013","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0235","series-title":"PhD Thesis, Biogen\u00e8se des prot\u00e9ines \u00e0 centre [FeS] et leur r\u00f4le dans la thiolation des ARNts","author":"L\u00e9non","year":"2023"},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0240","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1042\/bst0300680","article-title":"Biosynthesis of iron-Sulphur clusters is a complex and highly conserved process","volume":"30","author":"Frazzon","year":"2002","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.jinorgbio.2025.113064_bb0245","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/0076-6879(88)58067-9","article-title":"Rapid colorimetric micromethod for the quantitation of complexed Iron in biological samples","volume":"158","author":"Fish","year":"1988","journal-title":"Methods Enzymol."}],"updated-by":[{"DOI":"10.1016\/j.jinorgbio.2025.113096","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000}}],"container-title":["Journal of Inorganic Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0162013425002442?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0162013425002442?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T14:29:44Z","timestamp":1780410584000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0162013425002442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":49,"alternative-id":["S0162013425002442"],"URL":"https:\/\/doi.org\/10.1016\/j.jinorgbio.2025.113064","relation":{},"ISSN":["0162-0134"],"issn-type":[{"value":"0162-0134","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The thiolation of U34 at carbon 2 in tRNA by Escherichia coli MnmA precedes modification at carbon 5 and is dependent on a [4Fe-4S] cluster","name":"articletitle","label":"Article Title"},{"value":"Journal of Inorganic Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinorgbio.2025.113064","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"113064"}}