{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:33:10Z","timestamp":1792665190326,"version":"4.5.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Insect Physiology"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.jinsphys.2019.103997","type":"journal-article","created":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T02:02:05Z","timestamp":1576288925000},"page":"103997","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Mechanisms of transport of H+, Na+ and K+, across the distal gastric caecum of larval Aedes aegypti"],"prefix":"10.1016","volume":"121","author":[{"given":"N.M.","family":"D'Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.J.","family":"O'Donnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jinsphys.2019.103997_b0005","doi-asserted-by":"crossref","first-page":"6632","DOI":"10.1021\/bi00395a011","article-title":"Subunit composition and ATP site labeling of the coated vesicle proton-translocating adenosinetriphosphatase","volume":"26","author":"Arai","year":"1987","journal-title":"Biochemistry"},{"key":"10.1016\/j.jinsphys.2019.103997_b0010","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.jinsphys.2011.11.015","article-title":"The blowfly salivary gland - a model system for analyzing the regulation of plasma membrane V-ATPase","volume":"58","author":"Baumann","year":"2012","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0015","first-page":"145","article-title":"Energizing epithelial transport with the vacuolar H+-ATPase","volume":"16","author":"Beyenbach","year":"2001","journal-title":"News Physiol Sci"},{"key":"10.1016\/j.jinsphys.2019.103997_b0020","doi-asserted-by":"crossref","first-page":"3845","DOI":"10.1242\/jeb.00639","article-title":"Transport mechanisms of diuresis in Malpighian tubules of insects","volume":"206","author":"Beyenbach","year":"2003","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0025","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1242\/jeb.203.9.1459","article-title":"Central role of the apical membrane H+-ATPase in electrogenesis and epithelial transport in Malpighian tubules","volume":"203","author":"Beyenbach","year":"2000","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0030","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1242\/jeb.204.4.691","article-title":"In situ analysis of pH gradients in mosquito larvae using non-invasive, self-referencing, pH-sensitive microelectrodes","volume":"204","author":"Boudko","year":"2001","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0035","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/BF01871190","article-title":"H+-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cells","volume":"94","author":"Bowman","year":"1986","journal-title":"J. Membr. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0040","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1074\/jbc.M109756200","article-title":"Mutations in subunit C of the vacuolar ATPase confer resistance to bafilomycin and identify a conserved antibiotic binding site","volume":"277","author":"Bowman","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0045","doi-asserted-by":"crossref","first-page":"C803","DOI":"10.1152\/ajpcell.1992.262.4.C803","article-title":"Chemical probes for anion transporters of mammalian cell membranes","volume":"262","author":"Cabantchik","year":"1992","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0050","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/0022-1910(95)00124-7","article-title":"Stimulation of Malpighian tubules from larval Aedes aegypti by secretagogues","volume":"42","author":"Clark","year":"1996","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0055","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/(SICI)1520-6327(1997)34:2<123::AID-ARCH1>3.0.CO;2-Y","article-title":"Malpighian tubules of larval Aedes aegypti are hormonally stimulated by 5-hydroxytryptamine in response to increased salinity","volume":"34","author":"Clark","year":"1997","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0060","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1242\/jeb.202.3.247","article-title":"The anterior and posterior \u2018stomach\u2019regions of larval Aedes aegypti midgut: regional specialization of ion transport and stimulation by 5-hydroxytryptamine","volume":"202","author":"Clark","year":"1999","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0065","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1242\/jeb.204.10.1795","article-title":"The Drosophila melanogaster homologue of an insect calcitonin-like diuretic peptide stimulates V-ATPase activity in fruit fly Malpighian tubules","volume":"204","author":"Coast","year":"2001","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0070","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.jinsphys.2017.01.019","article-title":"The roles of V-type H+-ATPase and Na+\/K+-ATPase in energizing K+ and H+ transport in larval Drosophila gut epithelia","volume":"98","author":"D'Silva","year":"2017","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0075","article-title":"The gastric caecum of larval Aedes aegypti: stimulation of epithelial ion transport by 5-hydroxytryptamine and cAMP","volume":"221","author":"D'Silva","year":"2018","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0080","first-page":"3172","article-title":"Effects of rearing salinity on expression and function of ion motive ATPases and ion transport across the gastric caecum of Aedes aegypti larvae","volume":"220","author":"D'Silva","year":"2017","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0085","doi-asserted-by":"crossref","first-page":"3926","DOI":"10.1073\/pnas.0600011103","article-title":"cAMP regulates plasma membrane vacuolar-type H+-ATPase assembly and activity in blowfly salivary glands","volume":"103","author":"Dames","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jinsphys.2019.103997_b0090","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1086\/501059","article-title":"Secretion of water and ions by malpighian tubules of larval mosquitoes: effects of diuretic factors, second messengers, and salinity","volume":"79","author":"Donini","year":"2006","journal-title":"Physiol. Biochem. Zool."},{"key":"10.1016\/j.jinsphys.2019.103997_b0095","doi-asserted-by":"crossref","first-page":"19769","DOI":"10.1016\/S0021-9258(19)88619-2","article-title":"A novel mechanism for regulation of vacuolar acidification","volume":"267","author":"Feng","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0100","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1046\/j.1365-2583.2003.00415.x","article-title":"Expression of an Aedes aegypti cation-chloride cotransporter and its Drosophila homologues","volume":"12","author":"Filippov","year":"2003","journal-title":"Insect Mol. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0105","doi-asserted-by":"crossref","first-page":"R828","DOI":"10.1152\/ajpregu.00561.2013","article-title":"Serotonin triggers cAMP and PKA-mediated intracellular calcium waves in Malpighian tubules of Rhodnius prolixus","volume":"307","author":"Gioino","year":"2014","journal-title":"Am. J. Physiol.-Regulatory Integrative Compar. Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0110","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/0006-291X(90)92172-V","article-title":"Kinetic studies of chromaffin granule H+-ATPase and effects of bafilomycin A1","volume":"170","author":"Hanada","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jinsphys.2019.103997_b0115","doi-asserted-by":"crossref","first-page":"40544","DOI":"10.1074\/jbc.M207345200","article-title":"Concanamycin A, the specific inhibitor of V-ATPases, binds to the V(o) subunit c","volume":"277","author":"Huss","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0120","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/0022-1910(68)90092-9","article-title":"The fine structure of the gastric caeca of Aedes aegypti larvae","volume":"14","author":"Jones","year":"1968","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0125","doi-asserted-by":"crossref","first-page":"F1501","DOI":"10.1152\/ajprenal.00487.2005","article-title":"NHE8 mediates amiloride-sensitive Na+\/H+ exchange across mosquito Malpighian tubules and catalyzes Na+ and K+ transport in reconstituted proteoliposomes","volume":"292","author":"Kang'ethe","year":"2007","journal-title":"Am. J. Physiol. Renal Physiol"},{"key":"10.1016\/j.jinsphys.2019.103997_b0130","doi-asserted-by":"crossref","first-page":"12850","DOI":"10.1016\/S0021-9258(18)67170-4","article-title":"Characterization of the subunit structure of the maize tonoplast ATPase. Immunological and inhibitor binding studies","volume":"261","author":"Mandala","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0135","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1146\/annurev.bi.55.070186.003311","article-title":"Acidification of the endocytic and exocytic pathways","volume":"55","author":"Mellman","year":"1986","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0140","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-3-540-37843-3_4","article-title":"Electrochemical sensor applications to the study of molecular physiology and analyte flux in plants","author":"Messerli","year":"2006","journal-title":"Plant Electrophysiol. Springer"},{"key":"10.1016\/j.jinsphys.2019.103997_b0145","doi-asserted-by":"crossref","first-page":"14723","DOI":"10.1016\/S0021-9258(18)47855-6","article-title":"Nucleotide binding sites and chemical modification of the chromaffin granule proton ATPase","volume":"262","author":"Moriyama","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0150","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1242\/jeb.057828","article-title":"Salt stress alters fluid and ion transport by Malpighian tubules of Drosophila melanogaster: evidence for phenotypic plasticity","volume":"214","author":"Naikkhwah","year":"2011","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0155","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1242\/jeb.199.5.1163","article-title":"Separate control of anion and cation transport in Malpighian tubules of Drosophila melanogaster","volume":"199","author":"O'Donnell","year":"1996","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0160","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1242\/jeb.01208","article-title":"The anterior stomach of larval mosquitoes (Aedes aegypti): effects of neuropeptides on transepithelial ion transport and muscular motility","volume":"207","author":"Onken","year":"2004","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0165","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1242\/jeb.00964","article-title":"The transepithelial voltage of the isolated anterior stomach of mosquito larvae (Aedes aegypti): pharmacological characterization of the serotonin-stimulated cells","volume":"207","author":"Onken","year":"2004","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1673\/031.008.4601","article-title":"Alkalinization in the isolated and perfused anterior midgut of the larval mosquito, Aedes aegypti","volume":"8","author":"Onken","year":"2008","journal-title":"J. Insect Sci."},{"key":"10.1016\/j.jinsphys.2019.103997_b0175","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jinsphys.2013.12.008","article-title":"Transport of H+, Na+ and K+ across the posterior midgut of blood-fed mosquitoes (Aedes aegypti)","volume":"61","author":"Pacey","year":"2014","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0180","doi-asserted-by":"crossref","first-page":"4638","DOI":"10.1242\/jeb.02551","article-title":"P-type Na+\/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti","volume":"209","author":"Patrick","year":"2006","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0185","doi-asserted-by":"crossref","first-page":"F730","DOI":"10.1152\/ajprenal.90564.2008","article-title":"NHE8 is an intracellular cation\/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti","volume":"296","author":"Piermarini","year":"2009","journal-title":"Am. J. Physiol. Renal Physiol"},{"key":"10.1016\/j.jinsphys.2019.103997_b0190","doi-asserted-by":"crossref","first-page":"3529","DOI":"10.1242\/jeb.02419","article-title":"Molecular characterization of sodium\/proton exchanger 3 (NHE3) from the yellow fever vector, Aedes aegypti","volume":"209","author":"Pullikuth","year":"2006","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0195","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1242\/jeb.27.2.145","article-title":"Osmotic regulation in mosquito larvae","volume":"27","author":"Ramsay","year":"1950","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0200","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1006\/bbrc.1993.1783","article-title":"Purification of vacuolar ATPase with bafilomycin C1 affinity chromatography","volume":"194","author":"Rautiala","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jinsphys.2019.103997_b0205","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1242\/jeb.007872","article-title":"Molecular cloning, phylogeny and localization of AgNHA1: the first Na+\/H+ antiporter (NHA) from a metazoan, Anopheles gambiae","volume":"210","author":"Rheault","year":"2007","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0210","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jinsphys.2015.09.007","article-title":"Secretion of Na+, K+ and fluid by the Malpighian (renal) tubule of the larval cabbage looper Trichoplusia ni (Lepidoptera: Noctuidae)","volume":"82","author":"Ruiz-Sanchez","year":"2015","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0215","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1242\/jeb.00932","article-title":"Mechanisms of K+ transport across basolateral membranes of principal cells in Malpighian tubules of the yellow fever mosquito, Aedes aegypti","volume":"207","author":"Scott","year":"2004","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0220","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1242\/jeb.008342","article-title":"Cloning and characterization of AgCA9, a novel \u03b1-carbonic anhydrase from Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) larvae","volume":"210","author":"Smith","year":"2007","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0225","doi-asserted-by":"crossref","first-page":"16618","DOI":"10.1074\/jbc.272.26.16618","article-title":"Reconstitution in Vitro of the V1 Complex from the Yeast Vacuolar Proton-translocating ATPase assembly recapitulates mechanism","volume":"272","author":"Tomashek","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jinsphys.2019.103997_b0230","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0040-8166(89)90069-4","article-title":"Investigations on the midgut caeca of mosquito larvae-I. Fine structure","volume":"21","author":"Volkmann","year":"1989","journal-title":"Tissue Cell"},{"key":"10.1016\/j.jinsphys.2019.103997_b0235","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0040-8166(89)90070-0","article-title":"Investigations on the midgut caeca of mosquito larvae-II","volume":"21","author":"Volkmann","year":"1989","journal-title":"Functional aspects. Tissue Cell"},{"key":"10.1016\/j.jinsphys.2019.103997_b0240","doi-asserted-by":"crossref","first-page":"C1047","DOI":"10.1152\/ajpcell.00328.2009","article-title":"Calcineurin activity augments cAMP\/PKA-dependent activation of V-ATPase in blowfly salivary glands","volume":"298","author":"Voss","year":"2010","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0245","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1023\/A:1005448614450","article-title":"The plasma membrane H+-V-ATPase from tobacco hornworm midgut","volume":"31","author":"Wieczorek","year":"1999","journal-title":"J. Bioenerg. Biomembr."},{"key":"10.1016\/j.jinsphys.2019.103997_b0250","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1242\/jeb.10.1.16","article-title":"The function of the anal gills of the mosquito larva","volume":"10","author":"Wigglesworth","year":"1933","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0255","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1242\/jeb.19.1.56","article-title":"The storage of protein, fat, glycogen and uric acid in the fat body and other tissues of mosquito larvae","volume":"1942","author":"Wigglesworth","year":"1942","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2019.103997_b0260","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.jinsphys.2011.12.009","article-title":"Localization of two Na+- or K+-H+ antiporters, AgNHA1 and AgNHA2, in Anopheles gambiae larval Malpighian tubules and the functional expression of AgNHA2 in yeast","volume":"58","author":"Xiang","year":"2012","journal-title":"J. Insect Physiol."}],"container-title":["Journal of Insect Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022191019304123?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022191019304123?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:47:31Z","timestamp":1758300451000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022191019304123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":52,"alternative-id":["S0022191019304123"],"URL":"https:\/\/doi.org\/10.1016\/j.jinsphys.2019.103997","relation":{},"ISSN":["0022-1910"],"issn-type":[{"value":"0022-1910","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mechanisms of transport of H+, Na+ and K+, across the distal gastric caecum of larval Aedes aegypti","name":"articletitle","label":"Article Title"},{"value":"Journal of Insect Physiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinsphys.2019.103997","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103997"}}