{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:21Z","timestamp":1787458941588,"version":"build-2736575974"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Insect Physiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jinsphys.2026.105040","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:58:14Z","timestamp":1785340694000},"page":"105040","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Comprehensive proteomic analysis of honeybee (Apis mellifera L.) intestine reveals nutrient-dependent molecular adaptation"],"prefix":"10.1016","volume":"173","author":[{"given":"Valentina","family":"Ciaravolo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovica","family":"Cimmino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Santopietro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Scaloni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Arena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennaro","family":"Di Prisco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jinsphys.2026.105040_bb0005","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1023\/A:1006456720416","article-title":"Varroa jacobsoni (Acari: Varroidae) is more than one species","volume":"24","author":"Anderson","year":"2000","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0010","doi-asserted-by":"crossref","first-page":"3414","DOI":"10.1002\/pmic.201000321","article-title":"Modern proteomic methodologies for the characterization of lactosylation protein targets in milk","volume":"10","author":"Arena","year":"2010","journal-title":"Proteomics"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0015","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-53357-w","article-title":"Distribution of infectious and parasitic agents among three sentinel bee species across European agricultural landscapes","volume":"14","author":"Babin","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0020","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2013.00202","article-title":"Eat to reproduce: a key role for the insulin signaling pathway in adult insects","volume":"4","author":"Badisco","year":"2013","journal-title":"Front. Physiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0025","doi-asserted-by":"crossref","DOI":"10.3390\/insects16070741","article-title":"The role of heat shock proteins in insect stress response, immunity, and climate adaptation","volume":"16","author":"Banfi","year":"2025","journal-title":"Insects"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0030","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21144955","article-title":"Black soldier fly larvae adapt to different food substrates through morphological and functional responses of the midgut","volume":"21","author":"Bonelli","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0035","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1101\/gr.155994.113","article-title":"Honey bee protein atlas at organ-level resolution","volume":"23","author":"Chan","year":"2013","journal-title":"Genome Res."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0040","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1603\/022.038.0302","article-title":"Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France","volume":"38","author":"Chauzat","year":"2009","journal-title":"Environ. Entomol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0045","article-title":"Comparative transcriptome analysis reveals the effects of a high-protein diet on silkworm midgut","volume":"16","author":"Chen","year":"2025","journal-title":"Insects"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0050","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.1059001","article-title":"Changes in gut microbiota and metabolism associated with phenotypic plasticity in the honey bee Apis mellifera","volume":"13","author":"Copeland","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0055","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1093\/jee\/tow012","article-title":"Parasaccharibacter apium, gen. Nov., sp. Nov., improves honey bee (Hymenoptera: Apidae) resistance to Nosema","volume":"109","author":"Corby-Harris","year":"2016","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0060","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-07635-0","article-title":"Density of invasive western honey bee (Apis mellifera) colonies in fragmented woodlands indicates potential for large impacts on native species","volume":"12","author":"Cunningham","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0065","article-title":"Proteomic analysis in the Dufour\u2019s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)","volume":"12","author":"das Dores Teixeira","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0070","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3896\/IBRA.1.49.1.30","article-title":"Colony losses, managed colony population decline, and Colony collapse disorder in the United States","volume":"49","author":"Ellis","year":"2010","journal-title":"J. Apic. Res."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0075","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1111\/j.1365-2583.2006.00682.x","article-title":"Immune pathways and defence mechanisms in honey bees Apis mellifera","volume":"15","author":"Evans","year":"2006","journal-title":"Insect Mol. Biol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0080","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00253-010-2573-8","article-title":"Honey bee pathology: current threats to honey bees and beekeeping","volume":"87","author":"Genersch","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0085","doi-asserted-by":"crossref","unstructured":"Hoffmann, J.A., 1995. Innate immunity of insects. Curr. Opin. Immunol.. 7, 4\u201310. doi:10.1016\/0952-7915(95)80022-0.","DOI":"10.1016\/0952-7915(95)80022-0"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0090","first-page":"20172140","article-title":"The worldwide importance of honey bees as pollinators in natural habitats","volume":"285","author":"Hung","year":"2018","journal-title":"Proc. Biol. Sci."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0095","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.foodcont.2016.02.051","article-title":"Mixture-risk-assessment of pesticide residues in retail polyfloral honey","volume":"67","author":"Juan-Borr\u00e1s","year":"2016","journal-title":"Food Control"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0100","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-025-13615-x","article-title":"Dietary modulation of gut microbiota and functional enzymes in savannah honey bees (Apis mellifera scutellata Lepeletier)","volume":"109","author":"Khumalo","year":"2025","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0105","first-page":"303","article-title":"Importance of pollinators in changing landscapes for world crops","volume":"274","author":"Klein","year":"2007","journal-title":"Proc. Biol. Sci."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0110","doi-asserted-by":"crossref","first-page":"737","DOI":"10.3390\/nu15030737","article-title":"Determinants of honey and other bee products use for culinary, cosmetic, and medical purposes","volume":"15","author":"Kowalczuk","year":"2023","journal-title":"Nutrients"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0115","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1365-2583.2006.00677.x","article-title":"Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome","volume":"15","author":"Kunieda","year":"2006","journal-title":"Insect Mol. Biol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0120","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1038\/nrmicro.2016.43","article-title":"Gut microbial communities of social bees","volume":"14","author":"Kwong","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0125","doi-asserted-by":"crossref","DOI":"10.1093\/jisesa\/iead074","article-title":"Based proteomics analyses reveal response mechanisms of Apis mellifera (Hymenoptera: Apidae) against the heat stress","volume":"24","author":"Li","year":"2024","journal-title":"J. Insect Sci."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0130","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/wdev.51","article-title":"The Drosophila midgut: a model for stem cell driven tissue regeneration","volume":"1","author":"Lucchetta","year":"2012","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0135","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00018-005-5386-7","article-title":"The Nudix hydrolase superfamily","volume":"63","author":"McLennan","year":"2006","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0140","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.250507","article-title":"Moderate nutritional stress reprogrammes insulin responses to drive enhanced starvation tolerance in Drosophila melanogaster","volume":"229","author":"Menon","year":"2026","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.jinsphys.2026.105040_bib218","series-title":"Ecosystems and Human Well-being: Synthesis","author":"Millennium Ecosystem Assessment","year":"2005"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0145","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/s41579-023-00990-3","article-title":"The honeybee microbiota and its impact on health and disease","volume":"22","author":"Motta","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bib219","doi-asserted-by":"crossref","unstructured":"Neff, John & Simpson, Beryl. (2017). Vogel\u2019s Great Legacy: The Oil Flower and Oil-Collecting Bee Syndrome. Flora - Morphology, Distribution, Functional Ecology of Plants. 232. 10.1016\/j.flora.2017.01.003.","DOI":"10.1016\/j.flora.2017.01.003"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0150","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jip.2018.09.007","article-title":"Impact of the microsporidian Nosema ceranae on the gut epithelium renewal of the honeybee, Apis mellifera","volume":"159","author":"Panek","year":"2018","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0155","doi-asserted-by":"crossref","first-page":"9649","DOI":"10.1007\/s11357-014-9649-9","article-title":"The dietary proportion of essential amino acids and Sir2 influence lifespan in the honeybee","volume":"36","author":"Paoli","year":"2014","journal-title":"Age"},{"issue":"D1","key":"10.1016\/j.jinsphys.2026.105040_bb0160","doi-asserted-by":"crossref","first-page":"D543","DOI":"10.1093\/nar\/gkae1011","article-title":"The PRIDE database at 20 years: 2025 update","volume":"53","author":"Perez-Riverol","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0165","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tree.2010.01.007","article-title":"Global pollinator declines: trends, impacts and drivers","volume":"25","author":"Potts","year":"2010","journal-title":"Trends Ecol. Evol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0170","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/nature20588","article-title":"Safeguarding pollinators and their values to human well-being","volume":"540","author":"Potts","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0175","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1073\/pnas.1818371116","article-title":"Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph","volume":"116","author":"Ramsey","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1005180","article-title":"Windpipe controls drosophila intestinal homeostasis by regulating JAK\/STAT pathway via promoting receptor endocytosis and lysosomal degradation","volume":"11","author":"Ren","year":"2015","journal-title":"PLoS Genet."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0185","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.foodpol.2012.06.003","article-title":"Agricultural pesticides and land use intensification in high, middle and low income countries","volume":"37","author":"Schreinemachers","year":"2012","journal-title":"Food Policy"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0190","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-01547-1","article-title":"Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress","volume":"11","author":"Shih","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinsphys.2023.104601","article-title":"Honeybee symbiont Bombella apis could restore larval-to-pupal transition disrupted by antibiotic treatment","volume":"153","author":"Xiaowen","year":"2024","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0200","article-title":"Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides","volume":"10","author":"Zaluski","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0205","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2025.1617478","article-title":"Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells","volume":"13","author":"Zanotti","year":"2025","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.jinsphys.2026.105040_bb0210","article-title":"Insect behavior and physiological adaptation mechanisms under starvation stress","volume":"10","author":"Zhang","year":"2019","journal-title":"Front. Physiol."},{"key":"10.1016\/j.jinsphys.2026.105040_bib216","doi-asserted-by":"crossref","unstructured":"Zhang P, Edgar BA. Insect Gut Regeneration. Cold Spring Harb Perspect Biol. 2022 Feb 1; 14(2): a040915. doi: 10.1101\/cshperspect.a040915. PMID: 34312250; PMCID: PMC8805648.","DOI":"10.1101\/cshperspect.a040915"},{"key":"10.1016\/j.jinsphys.2026.105040_bb0215","article-title":"Current knowledge on bee innate immunity based on genomics and transcriptomics","volume":"23","author":"Zhao","year":"2022","journal-title":"Int. J. Mol. Sci."}],"container-title":["Journal of Insect Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022191026001137?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022191026001137?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:16:11Z","timestamp":1787458571000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022191026001137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":46,"alternative-id":["S0022191026001137"],"URL":"https:\/\/doi.org\/10.1016\/j.jinsphys.2026.105040","relation":{},"ISSN":["0022-1910"],"issn-type":[{"value":"0022-1910","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comprehensive proteomic analysis of honeybee (Apis mellifera L.) intestine reveals nutrient-dependent molecular adaptation","name":"articletitle","label":"Article Title"},{"value":"Journal of Insect Physiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jinsphys.2026.105040","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"105040"}}