{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:58:15Z","timestamp":1791853095556,"version":"4.3.4"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:00:00Z","timestamp":1697587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017163","name":"Statens veterin\u00e4rmedicinska anstalt","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017163","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024104","name":"Veterin\u00e6rinstituttets","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Invertebrate Pathology"],"published-print":{"date-parts":[[2023,11]]},"DOI":"10.1016\/j.jip.2023.108008","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T11:59:28Z","timestamp":1697630368000},"page":"108008","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Molecular detection of Aphanomyces astaci \u2013 An improved species specific qPCR assay"],"prefix":"10.1016","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8804-7994","authenticated-orcid":false,"given":"David A.","family":"Strand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Jinnerot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Asp\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satu","family":"Viljamaa-Dirks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirpa","family":"Heinikainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elin","family":"Rol\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trude","family":"Vr\u00e5lstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jip.2023.108008_b0005","doi-asserted-by":"crossref","first-page":"603","DOI":"10.20506\/rst.15.2.943","article-title":"Geographical spread of bacterial and fungal diseases of crustaceans","volume":"15","author":"Alderman","year":"1996","journal-title":"Revue Sci. Et Tech. De L Office Int. Des Epizooties"},{"key":"10.1016\/j.jip.2023.108008_b0010","doi-asserted-by":"crossref","DOI":"10.1186\/s12898-019-0224-1","article-title":"Ecological plasticity and commercial impact of invasive marbled crayfish populations in Madagascar","volume":"19","author":"Andriantsoa","year":"2019","journal-title":"BMC Ecology"},{"key":"10.1016\/j.jip.2023.108008_b0257","unstructured":"Anonymous 2018. Year Report Health monitoring, freshwater crayfish and eel, n.d.. Swedish.Report. National Veterinary Institute, Sweden."},{"key":"10.1016\/j.jip.2023.108008_b0015","doi-asserted-by":"crossref","first-page":"149","DOI":"10.3354\/dao02571","article-title":"Self-limiting outbreak of crayfish plague in an Austropotamobius pallipes population of a river basin in the Abruzzi region (central Italy)","volume":"103","author":"Caprioli","year":"2013","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0020","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3354\/dao03275","article-title":"Aphanomyces astaci genotypes involved in recent crayfish plague outbreaks in central Italy","volume":"130","author":"Caprioli","year":"2018","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0025","doi-asserted-by":"crossref","DOI":"10.3389\/fevo.2021.597585","article-title":"Real-time PCR assays for rapid identification of common Aphanomyces astaci genotypes","volume":"9","author":"Di Domenico","year":"2021","journal-title":"Front. Ecol. Evol."},{"key":"10.1016\/j.jip.2023.108008_b0030","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/S0953-7562(09)80716-8","article-title":"Physiological adaptation of an Aphanomyces astaci strain isolated from the freshwater crayfish Procambarus clarkii","volume":"99","author":"Di\u00e9guez-Uribeondo","year":"1995","journal-title":"Mycol. Res."},{"key":"10.1016\/j.jip.2023.108008_b0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0070157","article-title":"Prevalence of the crayfish plague pathogen Aphanomyces astaci in populations of the signal crayfish Pacifastacus leniusculus in France: evaluating the threat to native crayfish","volume":"8","author":"Filipova","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jip.2023.108008_b0040","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.vetmic.2014.02.020","article-title":"Microsatellite markers for direct genotyping of the crayfish plague pathogen Aphanomyces astaci (Oomycetes) from infected host tissues","volume":"170","author":"Grandjean","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jip.2023.108008_b0045","doi-asserted-by":"crossref","first-page":"541","DOI":"10.3391\/ai.2017.12.4.10","article-title":"Status of Pacifastacus leniusculus and its role in recent crayfish plague outbreaks in France: improving distribution and crayfish plague infection patterns","volume":"12","author":"Grandjean","year":"2017","journal-title":"Aquat. Invasions"},{"key":"10.1016\/j.jip.2023.108008_b0050","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/1471-2180-9-184","article-title":"Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci","volume":"9","author":"Hochwimmer","year":"2009","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.jip.2023.108008_b0055","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1051\/kmae\/2009025","article-title":"A review of the ever increasing threat to European crayfish from non-indigenous crayfish species","volume":"394\u2013395","author":"Holdich","year":"2009","journal-title":"Knowl. Manag. Aquat. Ecosyst."},{"key":"10.1016\/j.jip.2023.108008_b0060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0044-8486(94)90243-7","article-title":"Analysis of genetic diversity in the crayfish plague fungus, Aphanomyces astaci, by random amplification of polymorphic DNA","volume":"126","author":"Huang","year":"1994","journal-title":"Aquaculture"},{"key":"10.1016\/j.jip.2023.108008_b0065","doi-asserted-by":"crossref","first-page":"31","DOI":"10.5869\/fc.2020.v25-1.031","article-title":"Prevalence of the Crayfish Plague, Aphanomyces astaci Schikora, in Alien Crayfish Species in Japan","volume":"25","author":"Kamimura","year":"2020","journal-title":"Freshwater Crayfish"},{"key":"10.1016\/j.jip.2023.108008_b0070","doi-asserted-by":"crossref","DOI":"10.1051\/kmae\/2014007","article-title":"Continental-wide distribution of crayfish species in Europe: update and maps","author":"Kouba","year":"2014","journal-title":"Knowl. Manag. Aquat. Ecosyst."},{"key":"10.1016\/j.jip.2023.108008_b0075","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jip.2011.08.002","article-title":"Spiny-cheek crayfish Orconectes limosus carry a novel genotype of the crayfish plague pathogen Aphanomyces astaci","volume":"108","author":"Kozub\u00edkov\u00e1","year":"2011","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2023.108008_b0080","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3354\/dao02411","article-title":"Re-examination of the prevalence of Aphanomyces astaci in North American crayfish populations in Central Europe by TaqMan MGB real-time PCR","volume":"97","author":"Kozub\u00edkov\u00e1","year":"2011","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0085","series-title":"100 of the World\u2019s Worst Invasive Alien Species a Selection from the Global Invasive Speices Database","author":"Lowe","year":"2004"},{"key":"10.1016\/j.jip.2023.108008_b0090","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3354\/dao02955","article-title":"Prevalence of the pathogen Aphanomyces astaci in freshwater crayfish populations in Croatia","volume":"118","author":"Maguire","year":"2016","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0095","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1017\/S0031182018000227","article-title":"MtDNA allows the sensitive detection and haplotyping of the crayfish plague disease agent Aphanomyces astaci showing clues about its origin and migration","volume":"145","author":"Makkonen","year":"2018","journal-title":"Parasitology"},{"key":"10.1016\/j.jip.2023.108008_b0100","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-88704-8","article-title":"Tracing the origin of the crayfish plague pathogen, Aphanomyces astaci, to the Southeastern United States","volume":"11","author":"Martin-Torrijos","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jip.2023.108008_b0105","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1017\/S0031182019000283","article-title":"Improved method for genotyping the causative agent of crayfish plague (Aphanomyces astaci) based on mitochondrial DNA","volume":"146","author":"Minardi","year":"2019","journal-title":"Parasitology"},{"key":"10.1016\/j.jip.2023.108008_b0110","doi-asserted-by":"crossref","DOI":"10.1051\/kmae\/2022011","article-title":"Investigation of the first recent crayfish plague outbreak in Ireland and its subsequent spread in the Bruskey River and surrounding areas","author":"Mirimin","year":"2022","journal-title":"Knowl. Manag. Aquat. Ecosyst."},{"key":"10.1016\/j.jip.2023.108008_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.jip.2020.107390","article-title":"Crayfish plague in Czechia: outbreaks from novel sources and testing for chronic infections","volume":"173","author":"Mojzisova","year":"2020","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2023.108008_b0120","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3897\/neobiota.74.79087","article-title":"Long-term changes in the prevalence of the crayfish plague pathogen and its genotyping in invasive crayfish species in Czechia","volume":"74","author":"Mojzisova","year":"2022","journal-title":"Neobiota"},{"key":"10.1016\/j.jip.2023.108008_b0125","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jip.2017.09.004","article-title":"Recent acute crayfish mortality reveals Aphanomyces astaci presence in Bosnia and Herzegovina","volume":"150","author":"Mrugala","year":"2017","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2023.108008_b0130","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.vetmic.2004.01.019","article-title":"Detection of genomic DNA of the crayfish plague fungus Aphanomyces astaci (Oomycete) in clinical samples by PCR","volume":"100","author":"Oidtmann","year":"2004","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.jip.2023.108008_b0256","doi-asserted-by":"crossref","first-page":"53","DOI":"10.3354\/dao072053","article-title":"Detection of Aphanomyces astaci in North American crayfish by polymerase chain reaction","volume":"72","author":"Oidtmann","year":"2006","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0135","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3354\/dao02432","article-title":"Invasive crayfish and crayfish plague on the move: first detection of the plague agent Aphanomyces astaci in the Romanian Danube","volume":"98","author":"P\u00e2rvulescu","year":"2012","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0140","doi-asserted-by":"crossref","first-page":"654","DOI":"10.3391\/bir.2021.10.3.16","article-title":"Distribution of Aphanomyces astaci Schikora, 1906, the causative agent of crayfish plague, in the Plitvice Lakes National Park, Croatia","volume":"10","author":"Pavic","year":"2021","journal-title":"Bioinvasions Rec"},{"key":"10.1016\/j.jip.2023.108008_b0145","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s10750-016-2841-4","article-title":"First detection of the crayfish plague pathogen Aphanomyces astaci in South America: a high potential risk to native crayfish","volume":"781","author":"Peiro","year":"2016","journal-title":"Hydrobiologia"},{"key":"10.1016\/j.jip.2023.108008_b0150","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1002\/aqc.2970","article-title":"Procambarus clarkii (Girard, 1852) and crayfish plague as new threats for biodiversity in Indonesia","volume":"28","author":"Putra","year":"2018","journal-title":"Aquat. Conserv. Mar. Freshwat. Ecosyst."},{"key":"10.1016\/j.jip.2023.108008_b0155","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.biocon.2018.04.009","article-title":"Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague pathogen using environmental DNA","volume":"222","author":"Robinson","year":"2018","journal-title":"Biol. Conserv."},{"key":"10.1016\/j.jip.2023.108008_b0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3897\/neobiota.58.49358","article-title":"Simultaneous detection of native and invasive crayfish and Aphanomyces astaci from environmental DNA samples in a wide range of habitats in Central Europe","author":"Rusch","year":"2020","journal-title":"Neobiota"},{"key":"10.1016\/j.jip.2023.108008_b0165","doi-asserted-by":"crossref","first-page":"503","DOI":"10.3391\/ai.2012.7.4.007","article-title":"Crayfish plague pathogen detected in the Danube Delta - a potential threat to freshwater biodiversity in southeastern Europe","volume":"7","author":"Schrimpf","year":"2012","journal-title":"Aquat. Invasions"},{"key":"10.1016\/j.jip.2023.108008_b0170","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1111\/fwb.12112","article-title":"Absence of the crayfish plague pathogen (Aphanomyces astaci) facilitates coexistence of European and American crayfish in central Europe","volume":"58","author":"Schrimpf","year":"2013","journal-title":"Freshw. Biol."},{"key":"10.1016\/j.jip.2023.108008_b0175","first-page":"11","article-title":"The crayfish plague fungus: history and recent advances","volume":"12","author":"S\u00f6derh\u00e4ll","year":"1999","journal-title":"Freshw. Crayfish"},{"key":"10.1016\/j.jip.2023.108008_b0180","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.vetmic.2012.05.008","article-title":"Monitoring the spore dynamics of Aphanomyces astaci in the ambient water of latent carrier crayfish","volume":"160","author":"Strand","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jip.2023.108008_b0185","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/1365-2664.12218","article-title":"Detection of crayfish plague spores in large freshwater systems","volume":"51","author":"Strand","year":"2014","journal-title":"J. Appl. Ecol."},{"key":"10.1016\/j.jip.2023.108008_b0190","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1111\/1365-2664.13404","article-title":"Monitoring a Norwegian freshwater crayfish tragedy - eDNA snapshots of invasion, infection and extinction","volume":"56","author":"Strand","year":"2019","journal-title":"J. Appl. Ecol."},{"key":"10.1016\/j.jip.2023.108008_b0195","unstructured":"Strand, D.A., Rusch, J., Johnsen, S.I., Tarpai, A., Vr\u00e5lstad, T., 2019b. The surveillance programme for Aphanomyces astaci in Norway 2018. Annual Report. Norwegian Veterinary Institute."},{"key":"10.1016\/j.jip.2023.108008_b0200","series-title":"The Surveillance Programme for Aphanomyces astaci in Norway 2019","author":"Strand","year":"2020"},{"key":"10.1016\/j.jip.2023.108008_b0205","series-title":"The Surveillance Programme for Aphanomyces astaci in Norway 2020","author":"Strand","year":"2021"},{"key":"10.1016\/j.jip.2023.108008_b0210","series-title":"The Surveillance Programme for Aphanomyces astaci in Norway 2021","author":"Strand","year":"2022"},{"key":"10.1016\/j.jip.2023.108008_b0215","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3354\/dao02445","article-title":"PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake Egirdir in Turkey","volume":"98","author":"Svoboda","year":"2012","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.jip.2023.108008_b0220","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.vetmic.2011.06.012","article-title":"A comparative study of molecular diagnostic methods designed to detect the crayfish plague pathogen, Aphanomyces Astaci","volume":"153","author":"Tuffs","year":"2011","journal-title":"Veterinary Microbiol."},{"key":"10.1016\/j.jip.2023.108008_b0225","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/jfd.12955","article-title":"A tentative new species Aphanomyces fennicus sp. nov. interferes with molecular diagnostic methods for crayfish plague","volume":"42","author":"Viljamaa-Dirks","year":"2019","journal-title":"J. Fish Dis."},{"key":"10.1016\/j.jip.2023.108008_b0230","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.vetmic.2008.12.022","article-title":"A quantitative TaqMan (R) MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci","volume":"137","author":"Vr\u00e5lstad","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jip.2023.108008_b0250","unstructured":"WOAH, 2021. Infection with Aphanomyces astasci (crayfish plague). Chapter 2.2.2. Manual of Diagnostic Tests for Aquatic Animal. World Organization for Animal Health, World Organization for Animal Health."},{"key":"10.1016\/j.jip.2023.108008_b0240","series-title":"The surveillance programme for Aphanomyces astaci in Norway 2017","author":"Vr\u00e5lstad","year":"2018"},{"key":"10.1016\/j.jip.2023.108008_b0235","series-title":"The surveillance programme for Aphanomyces astaci in Norway 2016","author":"Vr\u00e5lstad","year":"2017"},{"key":"10.1016\/j.jip.2023.108008_b0245","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1007\/s10530-018-1886-x","article-title":"eDNA-based crayfish plague detection as practical tool for biomonitoring and risk assessment of A. astaci-positive crayfish populations","volume":"21","author":"Wittwer","year":"2019","journal-title":"Biol. Invasions"},{"key":"10.1016\/j.jip.2023.108008_b0255","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.mcp.2006.03.003","article-title":"Evaluation of minor groove binding probe and Taqman probe PCR assays: influence of mismatches and template complexity on quantification","volume":"20","author":"Yao","year":"2006","journal-title":"Mol. Cell. Probes"}],"container-title":["Journal of Invertebrate Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022201123001258?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022201123001258?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T21:39:35Z","timestamp":1762205975000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022201123001258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":53,"alternative-id":["S0022201123001258"],"URL":"https:\/\/doi.org\/10.1016\/j.jip.2023.108008","relation":{},"ISSN":["0022-2011"],"issn-type":[{"value":"0022-2011","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular detection of Aphanomyces astaci \u2013 An improved species specific qPCR assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Invertebrate Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jip.2023.108008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108008"}}