{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:08:28Z","timestamp":1790712508440,"version":"4.1.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Zhejiang Province Natural Science Foundation","doi-asserted-by":"publisher","award":["LGC21C180002"],"award-info":[{"award-number":["LGC21C180002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Invertebrate Pathology"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.jip.2026.108757","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:11:34Z","timestamp":1790377894000},"page":"108757","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Highly sensitive detection and identification of six viruses in shrimps by universal primer based multiplex PCR"],"prefix":"10.1016","volume":"220","author":[{"given":"Jin-Yu","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu-Ya","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Hou","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jip.2026.108757_bb0005","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1007\/s10499-020-00568-3","article-title":"Major viral diseases in culturable penaeid shrimps: a review","volume":"28","author":"Arulmoorthy","year":"2020","journal-title":"Aquac. Int."},{"key":"10.1016\/j.jip.2026.108757_bb0010","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0167-5877(98)00131-7","article-title":"Comparison of methods for estimation of individual-level prevalence based on pooled samples","volume":"39","author":"Cowling","year":"1999","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.jip.2026.108757_bb0015","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0065-3527(04)63006-5","article-title":"Genomic organization, biology, and diagnosis of Taura syndrome virus and Yellowhead virus of Penaeid shrimp","volume":"63","author":"Dhar","year":"2004","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.jip.2026.108757_bb0020","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1007\/s13197-014-1459-7","article-title":"A higher sensitivity and efficiency of common primer multiplex PCR assay in identification of meat origin using NADH dehydrogenase subunit 4 gene","volume":"52","author":"Hanapi","year":"2015","journal-title":"J. Food Sci. Technol. Mysore"},{"key":"10.1016\/j.jip.2026.108757_bb0025","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.2307\/2533075","article-title":"Exact confidence intervals for proportions estimated by group testing","volume":"52","author":"Hepworth","year":"1996","journal-title":"Biometrics"},{"key":"10.1016\/j.jip.2026.108757_bb0030","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1007\/s00253-012-4277-8","article-title":"A universal primer multiplex PCR method for typing of toxinogenic Pseudomonas aeruginosa","volume":"95","author":"Hui","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jip.2026.108757_bb0035","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1111\/are.12045","article-title":"Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection","volume":"45","author":"Jeeva","year":"2014","journal-title":"Aquac. Res."},{"key":"10.1016\/j.jip.2026.108757_bb0040","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.3390\/v13071378","article-title":"Co-infection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) and white spot syndrome virus (WSSV) in the wild crustaceans of Andaman and Nicobar archipelago, India","volume":"13","author":"Kandasamy","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.jip.2026.108757_bb0045","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.mcp.2007.12.005","article-title":"Multiplex RT-PCR assay for simultaneous detection of six viruses of penaeid shrimp","volume":"22","author":"Khawsak","year":"2007","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jip.2026.108757_bb0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2004.02.008","article-title":"RT-PCR detection of yellow head virus (YHV) infection in Penaeus monodon using dried haemolymph spots","volume":"119","author":"Kiatpathomchai","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jip.2026.108757_bb0055","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1111\/jfd.13083","article-title":"To pool or not to pool? Guidelines for pooling samples for use in surveillance testing of infectious diseases in aquatic animals","volume":"42","author":"Laurin","year":"2019","journal-title":"J. Fish Dis."},{"key":"10.1016\/j.jip.2026.108757_bb0060","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.aca.2020.06.001","article-title":"Single universal primer recombinase polymerase amplification-based lateral flow biosensor (SUP-RPA-LFB) for multiplex detection of genetically modified maize","volume":"1127","author":"Li","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jip.2026.108757_bb0065","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jip.2012.03.006","article-title":"Historic emergence, impact and current status of shrimp pathogens in the Americas","volume":"110","author":"Lightner","year":"2012","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2026.108757_bb0070","series-title":"Establishment and application of real-time PCR assays for six viruses in shrimps","author":"Liu","year":"2023"},{"key":"10.1016\/j.jip.2026.108757_bb0075","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.jip.2013.09.007","article-title":"Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay","volume":"114","author":"Liu","year":"2013","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2026.108757_bb0080","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jviromet.2018.10.009","article-title":"Detection and differentiation of five diarrhea related pig viruses utilizing a multiplex PCR assay","volume":"263","author":"Liu","year":"2019","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jip.2026.108757_bb0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcp.2021.101747","article-title":"Novel universal primer-pentaplex PCR assay based on chimeric primers for simultaneous detection of five common pig viruses associated with diarrhea","volume":"58","author":"Nan","year":"2021","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jip.2026.108757_bb0090","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1007\/s11033-011-1155-x","article-title":"Detection and genetic profiling of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in wild berried freshwater prawn, Macrobrachium rosenbergii collected for hatchery production","volume":"39","author":"Nita","year":"2012","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.jip.2026.108757_bb0095","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s13337-013-0180-x","article-title":"Association of dual viral infection with mortality of Pacific white shrimp (Litopenaeus vannamei) in culture ponds in India","volume":"25","author":"Otta","year":"2014","journal-title":"Virusdisease"},{"key":"10.1016\/j.jip.2026.108757_bb0100","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0044-8486(02)00421-0","article-title":"Similarity between the histopathology of white spot syndrome virus and yellow head syndrome virus and its relevance to diagnosis of YHV disease in the Americas","volume":"218","author":"Pantoja","year":"2003","journal-title":"Aquaculture"},{"key":"10.1016\/j.jip.2026.108757_bb0105","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1099\/vir.0.81127-0","article-title":"Purification and characterization of infectious myonecrosis virus of penaeid shrimp","volume":"87","author":"Poulos","year":"2006","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jip.2026.108757_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.738145","article-title":"Evaluation of a commercial SPF shrimp breeding program: resistance to infectious myonecrosis virus (IMNV), Taura syndrome virus (TSV), and white spot syndrome virus (WSSV) from laboratory challenges","volume":"554","author":"Prochaska","year":"2022","journal-title":"Aquaculture"},{"key":"10.1016\/j.jip.2026.108757_bb0115","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jip.2012.03.013","article-title":"Disease will limit future food supply from the global crustacean fishery and aquaculture sectors","volume":"110","author":"Stentiford","year":"2012","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2026.108757_bb0120","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1111\/j.1365-2761.2009.01055.x","article-title":"Molecular detection of three shrimp viruses and genetic variation of white spot syndrome virus in Hainan Province, China, in 2007","volume":"32","author":"Tan","year":"2009","journal-title":"J. Fish Dis."},{"key":"10.1016\/j.jip.2026.108757_bb0125","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3354\/dao02293","article-title":"Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in","volume":"93","author":"Tang","year":"2011","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.jip.2026.108757_bb0130","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jviromet.2003.09.021","article-title":"Quantitation of Taura syndrome virus by real-time RT-PCR with a TaqMan assay","volume":"115","author":"Tang","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jip.2026.108757_bb0135","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.aquaculture.2015.10.028","article-title":"Review of current disease threats for cultivated penaeid shrimp in Asia","volume":"452","author":"Thitamadee","year":"2016","journal-title":"Aquaculture"},{"key":"10.1016\/j.jip.2026.108757_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2021.114362","article-title":"Rapid detection of (DIV1) by recombinase polymerase amplification","volume":"300","author":"Tong","year":"2022","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jip.2026.108757_bb0145","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1865-1682.2011.01249.x","article-title":"The infectious hypodermal and haematopoietic necrosis virus: a brief review of what we do and do not know","volume":"59","author":"Vega-Heredia","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.jip.2026.108757_bb0150","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/v8010023","article-title":"Molecular mechanisms of white spot syndrome virus infection and perspectives on treatments","volume":"8","author":"Verbruggen","year":"2016","journal-title":"Viruses"},{"key":"10.1016\/j.jip.2026.108757_bb0155","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3354\/dao063107","article-title":"Polychaete worms--a vector for white spot syndrome virus (WSSV)","volume":"63","author":"Vijayan","year":"2005","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.jip.2026.108757_bb0160","first-page":"1623","article-title":"Prevalence of three viruses in Pacific white leg shrimp in Jiangsu Province and the analysis of coding sequence of five strains of infectious hypodermal and hematopoietic necrosis virus","volume":"41","author":"Wang","year":"2017","journal-title":"J. Fish. China"},{"key":"10.1016\/j.jip.2026.108757_bb0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.jip.2025.108354","article-title":"Accurate and rapid detection of six viral pathogens in penaeid shrimp by using a novel multiplex PCR","volume":"211","author":"Wang","year":"2025","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.jip.2026.108757_bb0170","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3354\/dao076077","article-title":"A multiplex RT-PCR for simultaneous differentiation of three viral pathogens of penaeid shrimp","volume":"76","author":"Xie","year":"2007","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.jip.2026.108757_bb0175","doi-asserted-by":"crossref","first-page":"e22900","DOI":"10.1371\/journal.pone.0022900","article-title":"A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis","volume":"7","author":"Xu","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jip.2026.108757_bb0180","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.mcp.2015.07.006","article-title":"Development of SYBR green and TaqMan quantitative real-time PCR assays for hepatopancreatic parvovirus (HPV) infecting Penaeus monodon in India","volume":"29","author":"Yadav","year":"2015","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jip.2026.108757_bb0185","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s12250-011-3157-6","article-title":"Prevalence of three shrimp viruses in Zhejiang Province in 2008","volume":"26","author":"Yu","year":"2011","journal-title":"Virol. Sin."},{"key":"10.1016\/j.jip.2026.108757_bb0190","doi-asserted-by":"crossref","first-page":"114822","DOI":"10.1016\/j.jviromet.2023.114822","article-title":"Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses","volume":"322","author":"Zhang","year":"2023","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jip.2026.108757_bb0195","first-page":"17","article-title":"Detection and analysis on five kinds of pathogens in white shrimp (Penaeus Vannamei) in Zhejiang Province","volume":"35","author":"Zheng","year":"2018","journal-title":"China Animal Health Inspection."},{"key":"10.1016\/j.jip.2026.108757_bb0200","first-page":"756","article-title":"Analysis on presence of virus in Penaeus vannamei shrimp larvae in Zhejiang Province in 2013","volume":"27","author":"Zhu","year":"2015","journal-title":"Acta Agric. Zhejiang."},{"key":"10.1016\/j.jip.2026.108757_bb0205","doi-asserted-by":"crossref","first-page":"212","DOI":"10.3389\/fphys.2019.00212","article-title":"Identification of functional gene modules associated with STAT-mediated antiviral responses to white spot syndrome virus in shrimp","volume":"10","author":"Zhu","year":"2019","journal-title":"Front. Physiol."}],"container-title":["Journal of Invertebrate Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022201126002338?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022201126002338?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:23:52Z","timestamp":1790709832000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022201126002338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":41,"alternative-id":["S0022201126002338"],"URL":"https:\/\/doi.org\/10.1016\/j.jip.2026.108757","relation":{},"ISSN":["0022-2011"],"issn-type":[{"value":"0022-2011","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Highly sensitive detection and identification of six viruses in shrimps by universal primer based multiplex PCR","name":"articletitle","label":"Article Title"},{"value":"Journal of Invertebrate Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jip.2026.108757","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"108757"}}