{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:39:26Z","timestamp":1787315966537,"version":"build-2736575974"},"reference-count":40,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T00:00:00Z","timestamp":1739664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072318"],"award-info":[{"award-number":["82072318"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272380"],"award-info":[{"award-number":["82272380"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2305301"],"award-info":[{"award-number":["2022YFC2305301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2301002"],"award-info":[{"award-number":["2021YFC2301002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection and Public Health"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.jiph.2025.102708","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T12:47:45Z","timestamp":1739969265000},"page":"102708","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Uncovering the unseen: Metagenomic next-generation sequencing improves liver abscess diagnostics"],"prefix":"10.1016","volume":"18","author":[{"given":"Jing","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4174-755X","authenticated-orcid":false,"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Ya","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Yu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ting","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Fei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Hua","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Yu","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Wen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.jiph.2025.102708_bib1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.4269\/ajtmh.16-0228","article-title":"Burden of liver abscess and survival risk score in Thailand: a population-based study","author":"Poovorawan","year":"2016","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.jiph.2025.102708_bib2","article-title":"The epidemiology of hip fracture among subjects with pyogenic liver abscess (PLA): a nationwide population-based study","author":"Hsu","year":"2020","journal-title":"Biomed Res Int"},{"key":"10.1016\/j.jiph.2025.102708_bib3","doi-asserted-by":"crossref","DOI":"10.1038\/srep35890","article-title":"Clinical charateristics and outcome of pyogenic liver abscess with different size: 15-year experience from a single center","author":"Du","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.jiph.2025.102708_bib4","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/4752880","article-title":"Pyogenic liver abscess: contemporary trends in a tertiary institute","author":"Boaz","year":"2022","journal-title":"Int J Clin Pract"},{"issue":"8","key":"10.1016\/j.jiph.2025.102708_bib5","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.4103\/jfmpc.jfmpc_927_20","article-title":"Pyogenic liver abscess: clinical features and microbiological profiles in tertiary care center","author":"Kumar","year":"2020","journal-title":"J Fam Med Prim Care"},{"key":"10.1016\/j.jiph.2025.102708_bib6","article-title":"Pyogenic liver abscess risk in patients with newly diagnosed type 2 diabetes mellitus: a nationwide, population-based cohort study","author":"Wang","year":"2021","journal-title":"Front Med (Lausanne)"},{"issue":"8","key":"10.1016\/j.jiph.2025.102708_bib7","doi-asserted-by":"crossref","DOI":"10.1177\/0300060520949404","article-title":"Analysis of the clinical characteristics of 202 patients with liver abscess associated with diabetes mellitus and biliary tract disease","author":"Song","year":"2020","journal-title":"J Int Med Res"},{"issue":"3","key":"10.1016\/j.jiph.2025.102708_bib8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.4254\/wjh.v16.i3.316","article-title":"Amebic liver abscess: an update","author":"Kumar","year":"2024","journal-title":"World J Hepatol"},{"issue":"2","key":"10.1016\/j.jiph.2025.102708_bib9","first-page":"158","article-title":"The evolving nature of hepatic abscess: a review","author":"Mavilia","year":"2016","journal-title":"J Clin Transl Hepatol"},{"issue":"11","key":"10.1016\/j.jiph.2025.102708_bib10","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1086\/425616","article-title":"Pyogenic liver abscess: recent trends in etiology and mortality","author":"Rahimian","year":"2004","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jiph.2025.102708_bib11","article-title":"Point-of-care ultrasound diagnosis of a pyogenic liver abscess in the emergency department","volume":"2","author":"Mcclure","year":"2021","journal-title":"J Am Coll Emerg Physicians Open"},{"issue":"2","key":"10.1016\/j.jiph.2025.102708_bib12","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.gtc.2020.01.013","article-title":"Pyogenic and Amebic Infections of the Liver","author":"Roediger","year":"2020","journal-title":"Gastroenterol Clin North Am"},{"issue":"3","key":"10.1016\/j.jiph.2025.102708_bib13","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s10140-020-01757-6","article-title":"Beyond pyogenic liver abscess: a comprehensive review of liver infections in emergency settings","author":"Nepal","year":"2020","journal-title":"Emerg Radio"},{"issue":"5","key":"10.1016\/j.jiph.2025.102708_bib14","first-page":"334","article-title":"Therapy of liver abscesses","author":"Lubbert","year":"2014","journal-title":"Viszeralmedizin"},{"issue":"3","key":"10.1016\/j.jiph.2025.102708_bib15","first-page":"224","article-title":"Antibiotic treatment durations for pyogenic liver abscesses: a systematic review","author":"Curran","year":"2023","journal-title":"J Assoc Med Microbiol Infect Dis Can"},{"issue":"4","key":"10.1016\/j.jiph.2025.102708_bib16","doi-asserted-by":"crossref","first-page":"343","DOI":"10.31486\/toj.23.0030","article-title":"Tuberculosis presenting as ruptured liver abscess and inferior vena cava thrombosis in a pediatric patient","author":"Jatoi","year":"2023","journal-title":"Ochsner J"},{"issue":"10","key":"10.1016\/j.jiph.2025.102708_bib17","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1007\/s10620-007-0194-6","article-title":"Microbiology and management of abdominal infections","author":"Brook","year":"2008","journal-title":"Dig Dis Sci"},{"issue":"3","key":"10.1016\/j.jiph.2025.102708_bib18","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.cmi.2018.05.016","article-title":"Impact of antibiotic administration on blood culture positivity at the beginning of sepsis: a prospective clinical cohort study","author":"Scheer","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jiph.2025.102708_bib19","article-title":"Diagnostic values of BALF metagenomic next-generation sequencing, BALF real-time PCR and serum BDG for Pneumocystis jirovecii pneumonia in HIV-infected patients","author":"Chen","year":"2024","journal-title":"Front Microbiol"},{"key":"10.1016\/j.jiph.2025.102708_bib20","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev-pathmechdis-012418-012751","article-title":"Clinical metagenomic next-generation sequencing for pathogen detection","author":"Gu","year":"2019","journal-title":"Annu Rev Pathol"},{"key":"10.1016\/j.jiph.2025.102708_bib21","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/j.ijid.2022.07.060","article-title":"Metagenomic next-generation sequencing for accurate diagnosis and management of lower respiratory tract infections","author":"Liang","year":"2022","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.jiph.2025.102708_bib22","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41564-018-0349-6","article-title":"Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease","volume":"4","author":"Blauwkamp","year":"2019","journal-title":"Nat Microbiol"},{"key":"10.1016\/j.jiph.2025.102708_bib23","first-page":"899","article-title":"The scheme for validation of clinical metagenomics sequencing assay","volume":"9","author":"Zhang","year":"2022","journal-title":"Chin J Lab Med"},{"key":"10.1016\/j.jiph.2025.102708_bib24","first-page":"65","article-title":"Suggestions for interpretation of pathogen metagenomic high-throughput sequencing reports","volume":"17","author":"Zhang","year":"2023","journal-title":"J. Clin.\u00a0Lab."},{"issue":"1","key":"10.1016\/j.jiph.2025.102708_bib25","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1080\/20009666.2020.1831745","article-title":"Epidemiological, clinical, microbiological, and risk factors of pyogenic liver abscess: an 18-years retrospective single-center analysis","author":"Alkomos","year":"2021","journal-title":"J Community Hosp Intern Med Perspect"},{"key":"10.1016\/j.jiph.2025.102708_bib26","first-page":"1092","article-title":"Construction and quality assurance of metagenomic next-generation sequencing platform in pathogen detection","author":"Zhang","year":"2023","journal-title":"Zhonghua Yi Xue Za Zhi"},{"issue":"6","key":"10.1016\/j.jiph.2025.102708_bib27","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1086\/313539","article-title":"Klebsiella pneumoniae liver abscess, endophthalmitis, and meningitis in a man with newly recognized diabetes mellitus","author":"Saccente","year":"1999","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jiph.2025.102708_bib28","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3389\/fped.2020.00111","article-title":"Pediatric liver abscess: trends in the incidence, etiology, and outcomes based on 20-years of experience at a tertiary center","author":"Yeh","year":"2020","journal-title":"Front Pediatr"},{"issue":"12","key":"10.1016\/j.jiph.2025.102708_bib29","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1097\/INF.0000000000003253","article-title":"Hypervirulent Klebsiella pneumoniae Infections in Pediatric Populations in Beijing (2017-2019): clinical characteristics, molecular epidemiology and antimicrobial susceptibility","author":"Li","year":"2021","journal-title":"Pediatr Infect Dis J"},{"issue":"1","key":"10.1016\/j.jiph.2025.102708_bib30","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1186\/s12879-024-09634-0","article-title":"Pyogenic liver abscess in pediatric populations in beijing (2008-2023)","author":"Xie","year":"2024","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.jiph.2025.102708_bib31","first-page":"606","article-title":"Analysis of clinical characteristics and pognostic factors in patients with bacterial liver abscess","volume":"05","author":"Chen","year":"2024","journal-title":"Chin J Clin"},{"issue":"5","key":"10.1016\/j.jiph.2025.102708_bib32","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.jmii.2014.08.028","article-title":"Epidemiology and clinical outcome of pyogenic liver abscess: an analysis from the National Health Insurance Research Database of Taiwan, 2000-2011","author":"Chen","year":"2016","journal-title":"J Microbiol Immunol Infect"},{"issue":"12","key":"10.1016\/j.jiph.2025.102708_bib33","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2023.48218","article-title":"Incidence of colorectal cancer in patients diagnosed with pyogenic liver abscess","author":"Suzuki","year":"2023","journal-title":"JAMA Netw Open"},{"issue":"5","key":"10.1016\/j.jiph.2025.102708_bib34","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1007\/s15010-022-01793-z","article-title":"Large retrospective study analysing predictive factors of primary treatment failure, recurrence and death in pyogenic liver abscesses","author":"Rossi","year":"2022","journal-title":"Infection"},{"issue":"1","key":"10.1016\/j.jiph.2025.102708_bib35","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/s12879-017-2543-1","article-title":"The role of Enterococcus spp. and multidrug-resistant bacteria causing pyogenic liver abscesses","author":"Mucke","year":"2017","journal-title":"BMC Infect Dis"},{"issue":"2","key":"10.1016\/j.jiph.2025.102708_bib36","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1002\/jhbp.174","article-title":"Predictors of therapy failure in a series of 741 adult pyogenic liver abscesses","author":"Lo","year":"2015","journal-title":"J Hepatobiliary Pancreat Sci"},{"issue":"5","key":"10.1016\/j.jiph.2025.102708_bib37","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1086\/590932","article-title":"Predictors of septic metastatic infection and mortality among patients with Klebsiella pneumoniae liver abscess","author":"Lee","year":"2008","journal-title":"Clin Infect Dis"},{"issue":"7","key":"10.1016\/j.jiph.2025.102708_bib38","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.joen.2007.03.008","article-title":"Comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of Fusobacterium nucleatum and Peptostreptococcus (micromonas) micros","author":"Ozok","year":"2007","journal-title":"J Endod"},{"issue":"4","key":"10.1016\/j.jiph.2025.102708_bib39","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.4269\/ajtmh.20-0074","article-title":"Factors associated with high rates of recurrence of amebic liver abscess (ALA) in North India","author":"Singh","year":"2021","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.jiph.2025.102708_bib40","doi-asserted-by":"crossref","DOI":"10.1590\/0037-8682-0665-2021","article-title":"Amoebic liver abscess","author":"Chang","year":"2022","journal-title":"Rev Soc Bras Med Trop"}],"container-title":["Journal of Infection and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1876034125000577?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1876034125000577?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T23:07:32Z","timestamp":1780873652000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1876034125000577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["S1876034125000577"],"URL":"https:\/\/doi.org\/10.1016\/j.jiph.2025.102708","relation":{},"ISSN":["1876-0341"],"issn-type":[{"value":"1876-0341","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Uncovering the unseen: Metagenomic next-generation sequencing improves liver abscess diagnostics","name":"articletitle","label":"Article Title"},{"value":"Journal of Infection and Public Health","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jiph.2025.102708","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences.","name":"copyright","label":"Copyright"}],"article-number":"102708"}}