{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:33:12Z","timestamp":1793075592799,"version":"4.5.0"},"reference-count":20,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA212097"],"award-info":[{"award-number":["R01CA212097"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UH2CA307747"],"award-info":[{"award-number":["UH2CA307747"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","journalofliquidbiopsy.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Liquid Biopsy"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.jlb.2026.100672","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:56:13Z","timestamp":1791320173000},"page":"100672","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A novel multiplex digital PCR assay for simultaneous quantification of androgen receptor amplification, mutations, and splice variants in cell-free nucleic acids from metastatic castration-resistant prostate cancer"],"prefix":"10.1016","volume":"14","author":[{"given":"Yijun","family":"Tian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodie","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Ionescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingsong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Soupir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish","family":"Kohli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jlb.2026.100672_bib1","first-page":"10","article-title":"Cancer statistics, 2025","volume":"75","author":"Siegel","year":"2025","journal-title":"CA Cancer J Clin"},{"issue":"2","key":"10.1016\/j.jlb.2026.100672_bib2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.eururo.2020.09.042","article-title":"EAU-EANM-ESTRO-ESUR-SIOG guidelines on prostate Cancer-2020 update. Part 1: screening, diagnosis, and local treatment with curative intent","volume":"79","author":"Mottet","year":"2021","journal-title":"Eur Urol"},{"issue":"17","key":"10.1016\/j.jlb.2026.100672_bib3","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1056\/NEJMc1803343","article-title":"Metastatic prostate cancer","volume":"378","author":"Sartor","year":"2018","journal-title":"N Engl J Med"},{"issue":"2","key":"10.1016\/j.jlb.2026.100672_bib4","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/14737159.2020.1707669","article-title":"Biomarkers of response to advanced prostate cancer therapy","volume":"20","author":"Iacovelli","year":"2020","journal-title":"Expert Rev Mol Diagn"},{"issue":"5","key":"10.1016\/j.jlb.2026.100672_bib5","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.cell.2015.05.001","article-title":"Integrative clinical genomics of advanced prostate cancer","volume":"161","author":"Robinson","year":"2015","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.jlb.2026.100672_bib6","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/s41588-018-0078-z","article-title":"The long tail of oncogenic drivers in prostate cancer","volume":"50","author":"Armenia","year":"2018","journal-title":"Nat Genet"},{"issue":"12","key":"10.1016\/j.jlb.2026.100672_bib7","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nrc4016","article-title":"Emerging mechanisms of resistance to androgen receptor inhibitors in prostate cancer","volume":"15","author":"Watson","year":"2015","journal-title":"Nat Rev Cancer"},{"issue":"4","key":"10.1016\/j.jlb.2026.100672_bib8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1634\/theoncologist.2019-0115","article-title":"Comprehensive analysis of AR alterations in circulating tumor DNA from patients with advanced prostate cancer","volume":"25","author":"Ledet","year":"2020","journal-title":"Oncologist"},{"issue":"11","key":"10.1016\/j.jlb.2026.100672_bib9","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1056\/NEJMoa1315815","article-title":"AR-V7 and resistance to enzalutamide and abiraterone in prostate cancer","volume":"371","author":"Antonarakis","year":"2014","journal-title":"N Engl J Med"},{"issue":"11","key":"10.1016\/j.jlb.2026.100672_bib10","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1001\/jamaoncol.2016.1828","article-title":"Association of AR-V7 on circulating tumor cells as a treatment-specific biomarker with outcomes and survival in castration-resistant prostate cancer","volume":"2","author":"Scher","year":"2016","journal-title":"JAMA Oncol"},{"issue":"2","key":"10.1016\/j.jlb.2026.100672_bib11","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cell.2018.05.036","article-title":"Structural alterations driving castration-resistant prostate cancer revealed by linked-read genome sequencing","volume":"174","author":"Viswanathan","year":"2018","journal-title":"Cell"},{"issue":"3","key":"10.1016\/j.jlb.2026.100672_bib12","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.cell.2018.06.039","article-title":"Genomic hallmarks and structural variation in metastatic prostate cancer","volume":"174","author":"Quigley","year":"2018","journal-title":"Cell"},{"issue":"10","key":"10.1016\/j.jlb.2026.100672_bib13","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1038\/nmeth.2633","article-title":"Absolute quantification by droplet digital PCR versus analog real-time PCR","volume":"10","author":"Hindson","year":"2013","journal-title":"Nat Methods"},{"issue":"6","key":"10.1016\/j.jlb.2026.100672_bib14","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1373\/clinchem.2013.206375","article-title":"The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments","volume":"59","author":"Huggett","year":"2013","journal-title":"Clin Chem"},{"key":"10.1016\/j.jlb.2026.100672_bib15","first-page":"56","article-title":"A prospective genome-wide study of prostate cancer metastases reveals association of wnt pathway activation and increased tumor copy number with metastatic castration-resistant prostate cancer","volume":"37","author":"Wang","year":"2018","journal-title":"EBioMedicine"},{"key":"10.1016\/j.jlb.2026.100672_bib16","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1472-6750-11-80","article-title":"RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers","volume":"11","author":"Dobosy","year":"2011","journal-title":"BMC Biotechnol"},{"key":"10.1016\/j.jlb.2026.100672_bib18","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1038\/s41598-019-40719-y","article-title":"Clinical utility of androgen receptor gene aberrations in circulating cell-free DNA as a biomarker for treatment of castration-resistant prostate cancer","volume":"9","author":"Sumiyoshi","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.jlb.2026.100672_bib19","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jmoldx.2024.01.014","article-title":"Assay validation of cell-free DNA shallow whole-genome sequencing to determine tumor fraction in advanced cancers","volume":"26","author":"Rickles-Young","year":"2024","journal-title":"J Mol Diagn"},{"issue":"21","key":"10.1016\/j.jlb.2026.100672_bib17","doi-asserted-by":"crossref","first-page":"5225","DOI":"10.3390\/cancers14215225","article-title":"A phase 1b adaptive androgen deprivation therapy trial in metastatic castration sensitive prostate cancer","volume":"14","author":"Zhang","year":"2022","journal-title":"Cancers (Basel)"},{"issue":"1","key":"10.1016\/j.jlb.2026.100672_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-73350-3","article-title":"Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples","volume":"10","author":"Wagner","year":"2020","journal-title":"Sci Rep"}],"container-title":["The Journal of Liquid Biopsy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2950195426002183?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2950195426002183?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:00:36Z","timestamp":1793073636000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2950195426002183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":20,"alternative-id":["S2950195426002183"],"URL":"https:\/\/doi.org\/10.1016\/j.jlb.2026.100672","relation":{},"ISSN":["2950-1954"],"issn-type":[{"value":"2950-1954","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel multiplex digital PCR assay for simultaneous quantification of androgen receptor amplification, mutations, and splice variants in cell-free nucleic acids from metastatic castration-resistant prostate cancer","name":"articletitle","label":"Article Title"},{"value":"The Journal of Liquid Biopsy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jlb.2026.100672","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V. on behalf of The International Society of Liquid Biopsy.","name":"copyright","label":"Copyright"}],"article-number":"100672"}}