{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:32:48Z","timestamp":1793305968142,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UM1 CA182876"],"award-info":[{"award-number":["UM1 CA182876"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 CA144034"],"award-info":[{"award-number":["R01 CA144034"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001348","name":"A*STAR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jlr.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Lipid Research"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jlr.2026.101138","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:45:05Z","timestamp":1789119905000},"page":"101138","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["A fast method to quantify free and esterified cholesterol in human plasma and disease models"],"prefix":"10.1016","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2662-4941","authenticated-orcid":false,"given":"Michaela","family":"Chocholou\u0161kov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7413-1164","authenticated-orcid":false,"given":"Wai Kin","family":"Tham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5375-9454","authenticated-orcid":false,"given":"Menglan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5674-6341","authenticated-orcid":false,"given":"Woon-Puay","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew-Kiat","family":"Heng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7289-9890","authenticated-orcid":false,"given":"David L.","family":"Silver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Torta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jlr.2026.101138_bib1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nrm2330","article-title":"Membrane lipids: where they are and how they behave","volume":"9","author":"van Meer","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jlr.2026.101138_bib2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.trsl.2017.06.006","article-title":"Lipidomics in translational research and the clinical significance of lipid-based biomarkers","volume":"189","author":"Stephenson","year":"2017","journal-title":"Transl Res."},{"key":"10.1016\/j.jlr.2026.101138_bib3","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s42255-019-0094-z","article-title":"Lipidomics needs more standardization","volume":"1","author":"Liebisch","year":"2019","journal-title":"Nat. Metab."},{"key":"10.1016\/j.jlr.2026.101138_bib4","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1152\/physrev.1983.63.4.1343","article-title":"Transport of cholesterol","volume":"63","author":"Norum","year":"1983","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.jlr.2026.101138_bib5","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1242\/jcs.164715","article-title":"Complemedistrintary probes reveal that phosphatidylserine is required for the proper transbilayer distribution of cholesterol","volume":"128","author":"Maekawa","year":"2015","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jlr.2026.101138_bib6","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1002\/j.2040-4603.2013.tb00519.x","article-title":"Bile formation and secretion","volume":"3","author":"Boyer","year":"2013","journal-title":"Compr. Physiol."},{"key":"10.1016\/j.jlr.2026.101138_bib7","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/ng0208-129","article-title":"A treasure trove for lipoprotein biology","volume":"40","author":"Lusis","year":"2008","journal-title":"Nat. Genet."},{"key":"10.1016\/j.jlr.2026.101138_bib8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cccn.2005.04.003","article-title":"Cholesteryl esters in malignancy","volume":"359","author":"Tosi","year":"2005","journal-title":"Clinica Chim. Acta"},{"key":"10.1016\/j.jlr.2026.101138_bib9","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1161\/01.ATV.0000054658.91146.64","article-title":"Cholesteryl ester transfer protein: a novel target for raising HDL and Inhibiting atherosclerosis, Arteriosclerosis","volume":"23","author":"Barter","year":"2003","journal-title":"Thromb. Vasc. Biol."},{"key":"10.1016\/j.jlr.2026.101138_bib10","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1056\/NEJM199801083380203","article-title":"The role of a common variant of the cholesteryl ester transfer protein gene in the progression of coronary atherosclerosis","volume":"338","author":"Kuivenhoven","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jlr.2026.101138_bib11","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1194\/jlr.M500059-JLR200","article-title":"Aggregated LDL and lipid dispersions induce lysosomal cholesteryl","volume":"46","author":"Griffin","year":"2005","journal-title":"J Lipid Res"},{"key":"10.1016\/j.jlr.2026.101138_bib12","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.jfda.2018.09.001","article-title":"Analytical methods for cholesterol quantification","volume":"27","author":"Li","year":"2019","journal-title":"J. Food Drug Anal."},{"key":"10.1016\/j.jlr.2026.101138_bib13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0165-022X(98)00036-0","article-title":"Fluorometric method for the enzymatic determination of cholesterol","volume":"38","author":"Amundson","year":"1999","journal-title":"J. Biochem. Biophys. Meth"},{"key":"10.1016\/j.jlr.2026.101138_bib14","first-page":"470","article-title":"Enzymatic determination of total serum cholesterol","volume":"20","author":"Allain","year":"1974","journal-title":"Clin. Cem"},{"key":"10.1016\/j.jlr.2026.101138_bib15","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1507\/endocrj1954.29.429","article-title":"Lecithin: cholesterol acyl transfer rate in hypothyroidism and hyperthyroidism","volume":"29","author":"Kametani","year":"1982","journal-title":"Endocrinol. Jpn."},{"key":"10.1016\/j.jlr.2026.101138_bib16","doi-asserted-by":"crossref","first-page":"14764","DOI":"10.1038\/s41598-018-33116-4","article-title":"Cholesterol is inefficiently converted to cholesteryl esters in the blood of cardiovascular disease patients","volume":"8","author":"Gerl","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jlr.2026.101138_bib17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.5455\/medarh.2018.72.103-107","article-title":"The ratio of unesterified\/esterified cholesterol is the major determinant of atherogenicity of lipoprotein fractions","volume":"72","author":"Bagheri","year":"2018","journal-title":"Med. Arch."},{"key":"10.1016\/j.jlr.2026.101138_bib18","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1194\/jlr.D007336","article-title":"A simple and sensitive enzymatic method for cholesterol quantification in macrophages and foam cells","volume":"51","author":"Robinet","year":"2010","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jlr.2026.101138_bib19","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1373\/clinchem.2010.158766","article-title":"Proposed serum cholesterol reference measurement procedure by gas chromatography-isotope dilution Mass spectrometry","volume":"57","author":"Edwards","year":"2011","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib20","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.14233\/ajchem.2014.15780","article-title":"Determination of total cholesterol in serum by gas chromatography-mass spectrometry","volume":"26","author":"Lian","year":"2014","journal-title":"Asian J. Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib21","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1093\/oxfordjournals.jbchem.a127988","article-title":"Gas chromatographic separation of sterols and its clinical application","volume":"56","author":"Kanai","year":"1964","journal-title":"J. Biochem."},{"key":"10.1016\/j.jlr.2026.101138_bib22","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1002\/jssc.201700833","article-title":"Quantification of total cholesterol in human milk by gas chromatography","volume":"41","author":"Beggio","year":"2018","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jlr.2026.101138_bib23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.bbalip.2005.12.007","article-title":"High throughput quantification of cholesterol and cholesteryl esters by electrospray ionization tandem mass spectrometry (ESI-MS\/MS)","volume":"1761","author":"Liebisch","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jlr.2026.101138_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2025.465742","article-title":"Fast and comprehensive lipidomic analysis using supercritical fluid chromatography coupled with low and high resolution mass spectrometry","volume":"1745","author":"Chocholou\u0161kov\u00e1","year":"2025","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jlr.2026.101138_bib25","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1007\/s13361-017-1756-2","article-title":"Simultaneous quantification of free cholesterol, Cholesteryl esters, and triglycerides without ester hydrolysis by UHPLC separation and In-source collision induced dissociation coupled MS\/MS","volume":"28","author":"Michael","year":"2017","journal-title":"J. Am. Mass Spectrom."},{"key":"10.1016\/j.jlr.2026.101138_bib26","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1007\/s00216-020-02473-3","article-title":"Validation of lipidomic quantitative methods based on lipid class separation \u2013 mass spectrometry: Comparison of supercritical fluid chromatography and hydrophilic interaction liquid chromatography","volume":"412","author":"Wolrab","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib27","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1177\/2047487315626547","article-title":"C-reactive protein and serum creatinine, but not haemoglobin A1c, are independent predictors of coronary heart disease risk in non-diabetic Chinese","volume":"23","author":"Salim","year":"2016","journal-title":"Eur. J. Prev. Cardiol."},{"key":"10.1016\/j.jlr.2026.101138_bib28","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1177\/2047487316676609","article-title":"Utility of genetic and non-genetic risk factors in predicting coronary heart disease in Singaporean Chinese","volume":"24","author":"Chang","year":"2017","journal-title":"Eur. J. Prev. Cardiol."},{"key":"10.1016\/j.jlr.2026.101138_bib29","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.1021\/acs.analchem.8b05013","article-title":"Quantification of cholesterol and cholesteryl Ester by direct flow injection high-resolution fourier transform Mass spectrometry utilizing species-specific response factors","volume":"91","author":"Horing","year":"2019","journal-title":"Anal Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib30","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1007\/s00216-021-03364-x","article-title":"A normalized signal calibration with a long-term reference improves the robustness of RPLC-MRM\/MS lipidomics in plasma","volume":"413","author":"Babiy","year":"2021","journal-title":"Anal Bional Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib31","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1194\/jlr.M079012","article-title":"Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma","volume":"58","author":"Bowden","year":"2017","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jlr.2026.101138_bib32","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1194\/jlr.M009449","article-title":"Lipidomics reveals a remarkable diversity of lipids in human plasma","volume":"51","author":"Quehenberger","year":"2010","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jlr.2026.101138_bib33","doi-asserted-by":"crossref","first-page":"8562","DOI":"10.1038\/s41467-024-52087-x","article-title":"Concordant inter-laboratory derived concentration of ceramides in human plasma reference material via authentic standards","volume":"15","author":"Torta","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jlr.2026.101138_bib34","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1194\/jlr.D119000393","article-title":"Shared reference materials harmonize lipidomics across MS-based detection platforms and laboratories","volume":"61","author":"Triebl","year":"2020","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jlr.2026.101138_bib35","doi-asserted-by":"crossref","first-page":"122367","DOI":"10.1016\/j.talanta.2021.122367","article-title":"Intra-laboratory comparison of four analytical platforms for lipidomic quantitation using hydrophilic interaction liquid chromatography or supercritical fluid chromatography coupled to quadrupole - time-of-flight mass spectrometry","volume":"231","author":"Chocholou\u0161kov\u00e1","year":"2021","journal-title":"Talanta"},{"key":"10.1016\/j.jlr.2026.101138_bib36","doi-asserted-by":"crossref","first-page":"P1706","DOI":"10.1074\/jbc.TM118.004165","article-title":"NPC intracellular cholesterol transporter 1 (NPC1)-mediated cholesterol export from lysosomes","volume":"294","author":"Pfeffer","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jlr.2026.101138_bib37","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bbalip.2004.08.005","article-title":"Niemann-Pick C research from mouse to gene","volume":"1685","author":"Pentchev","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jlr.2026.101138_bib38","doi-asserted-by":"crossref","first-page":"15201","DOI":"10.1073\/pnas.1103746108","article-title":"The mammalian target of rapamycin regulates cholesterol biosynthetic gene expression and exhibits a rapamycin-resistant transcriptional profile","volume":"108","author":"Wang","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jlr.2026.101138_bib39","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/embor.2013.5","article-title":"The multifaceted role of mTORC1 in the control of lipid metabolism","volume":"14","author":"Ricoult","year":"2013","journal-title":"EMBO Rep."},{"key":"10.1016\/j.jlr.2026.101138_bib40","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1152\/physrev.00026.2020","article-title":"Regulation and metabolic functions of mTORC1 and mTORC2","volume":"101","author":"Szwed","year":"2021","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.jlr.2026.101138_bib41","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1186\/s13023-022-02385-8","article-title":"The long-term effect of mTOR inhibition on lipid and glucose metabolism in Tuberous sclerosis complex: data from the Dutch TSC registry","volume":"17","author":"Mulder","year":"2022","journal-title":"Orphanet J. Rare Dis."},{"key":"10.1016\/j.jlr.2026.101138_bib42","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1021\/ed079p377","article-title":"Cholesterol biosynthesis: lanosterol to cholesterol","volume":"79","author":"Risley","year":"2002","journal-title":"J. Chem. Education"},{"key":"10.1016\/j.jlr.2026.101138_bib43","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/S0022-2275(20)38456-X","article-title":"Serum lathosterol concentration is an indicator of whole-body cholesterol synthesis in humans","volume":"29","author":"Kempen","year":"1988","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jlr.2026.101138_bib44","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/s13361-017-1829-2","article-title":"Easy, fast, and reproducible quantification of cholesterol and other lipids in human plasma by combined high resolution MSX and FTMS analysis","volume":"29","author":"Gallego","year":"2018","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.jlr.2026.101138_bib45","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1093\/ajcn\/59.2.364","article-title":"Fatty acid composition of serum cholesterol esters, and erythrocyte and platelet membranes as indicators of long-term adherence to fat-modified diets","volume":"59","author":"Sarkkinen","year":"1994","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.jlr.2026.101138_bib46","doi-asserted-by":"crossref","first-page":"1114.e22","DOI":"10.1093\/oxfordjournals.aje.a009074","article-title":"Fatty acids in serum cholesteryl esters as quantitative biomarkers of dietary intake in humans","volume":"145","author":"Zock","year":"1997","journal-title":"Am. J. Epidemiol."}],"container-title":["Journal of Lipid Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022227526001689?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022227526001689?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:19:50Z","timestamp":1793305190000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022227526001689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S0022227526001689"],"URL":"https:\/\/doi.org\/10.1016\/j.jlr.2026.101138","relation":{},"ISSN":["0022-2275"],"issn-type":[{"value":"0022-2275","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A fast method to quantify free and esterified cholesterol in human plasma and disease models","name":"articletitle","label":"Article Title"},{"value":"Journal of Lipid Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jlr.2026.101138","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101138"}}