{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:38:13Z","timestamp":1790289493623,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2004,8]]},"DOI":"10.1016\/j.jmb.2004.06.067","type":"journal-article","created":{"date-parts":[[2004,7,13]],"date-time":"2004-07-13T19:02:11Z","timestamp":1089745331000},"page":"1189-1204","source":"Crossref","is-referenced-by-count":43,"title":["Structural Mechanism Governing Cis and Trans Isomeric States and an Intramolecular Switch for Cis\/Trans Isomerization of a Non-proline Peptide Bond Observed in Crystal Structures of Scorpion Toxins"],"prefix":"10.1016","volume":"341","author":[{"given":"Rong-Jin","family":"Guan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Lin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Guang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Sundberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da-Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2004.06.067_bib1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0065-3233(08)60402-7","article-title":"Conformation of polypeptides and proteins","volume":"23","author":"Ramachandran","year":"1968","journal-title":"Advan. Protein Chem."},{"key":"10.1016\/j.jmb.2004.06.067_bib2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0022-2836(76)80019-8","article-title":"An explanation for the rare occurrence of cis peptide units in proteins and polypeptides","volume":"107","author":"Ramachandran","year":"1976","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib3","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0022-2836(90)90159-J","article-title":"Occurrence and role of cis peptide bonds in protein structures","volume":"214","author":"Stewart","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib4","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/1368","article-title":"Peptide bonds revisited","volume":"5","author":"Weiss","year":"1998","journal-title":"Nature Struct. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib5","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1006\/jmbi.1998.2459","article-title":"Non-proline cis peptide bonds in proteins","volume":"286","author":"Jabs","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib6","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1006\/jmbi.1999.3217","article-title":"Cis peptide bonds in proteins: residues involved, their conformations, interactions and locations","volume":"294","author":"Pal","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib7","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/87624","article-title":"Nonprolyl cis peptide bonds in unfolded proteins cause complex folding kinetics","volume":"8","author":"Pappenberger","year":"2001","journal-title":"Nature Struct. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib8","doi-asserted-by":"crossref","first-page":"19778","DOI":"10.1074\/jbc.M001251200","article-title":"The structural features of concanavalin A governing non-proline peptide isomerization","volume":"275","author":"Bouckaert","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2004.06.067_bib9","doi-asserted-by":"crossref","first-page":"14485","DOI":"10.1021\/bi990507q","article-title":"Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex","volume":"38","author":"Heroux","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2004.06.067_bib10","doi-asserted-by":"crossref","first-page":"14495","DOI":"10.1021\/bi990508i","article-title":"Crystal structure of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase with XMP, pyrophosphate, and two Mg(2+) ions bound: insights into the catalytic mechanism","volume":"38","author":"Heroux","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2004.06.067_bib11","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1021\/bi962180o","article-title":"Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes","volume":"36","author":"Ludwig","year":"1997","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2004.06.067_bib12","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1006\/jmbi.1998.2210","article-title":"Conformational analysis of the first observed non-proline cis-peptide bond occurring within the complementarily determining region (CDR) of an antibody","volume":"284","author":"Bates","year":"1998","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0022-2836(95)80039-5","article-title":"Non-prolyl cis\u2013trans peptide bond isomerization as a rate-determining step in protein unfolding and refolding","volume":"245","author":"Odefey","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib14","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1002\/(SICI)1097-0134(199605)25:1<104::AID-PROT8>3.3.CO;2-H","article-title":"The rate-limiting step in the folding of the cis-Pro167Thr mutant of TEM-1 beta-lactamase is the trans to cis isomerization of a non-proline peptide bond","volume":"25","author":"Vanhove","year":"1996","journal-title":"Proteins: Struct. Funct. Genet."},{"key":"10.1016\/j.jmb.2004.06.067_bib15","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1002\/(SICI)1097-0134(19981201)33:4<550::AID-PROT7>3.0.CO;2-9","article-title":"The slow step of folding of Staphylococcus aureus PC1 beta-lactamase involves the collapse of a surface loop rate limited by the trans to cis isomerization of a non-proline peptide bond","volume":"33","author":"Wheeler","year":"1998","journal-title":"Proteins: Struct. Funct. Genet."},{"key":"10.1016\/j.jmb.2004.06.067_bib16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1085\/jgp.74.3.375","article-title":"Binding of scorpion toxin to receptor site associated with sodium channels in for muscle","volume":"74","author":"Catterall","year":"1979","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.jmb.2004.06.067_bib17","unstructured":"Rochat, H., Berhard, P. & Couraud, F. (1979). Scorpion toxin chemistry and mode of action. In Advances in Cylopharmacologyl (Cecarelli, B. & Chementi, F., eds), vol. 3, pp. 325\u2013334, Raven Press, New York."},{"key":"10.1016\/j.jmb.2004.06.067_bib18","doi-asserted-by":"crossref","first-page":"8034","DOI":"10.1074\/jbc.271.14.8034","article-title":"Scorpion toxins affecting sodium current inactivation bind to distinct homologous receptor sites on rat brain and insect sodium channels","volume":"271","author":"Gordon","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2004.06.067_bib19","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1006\/jmbi.1996.0473","article-title":"Crystal structure of an acidic neurotoxin from scorpion Buthus martensii Karsch at 1.85\u00c5 resolution","volume":"261","author":"Li","year":"1996","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib20","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1006\/jmbi.1999.3036","article-title":"Crystal structures of two alpha-like scorpion toxins: non-proline cis peptide bonds and implications for new binding site selectivity on the sodium channel","volume":"292","author":"He","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib21","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1006\/jmbi.1994.1270","article-title":"Crystal structure of toxin II from the scorpion Androctonus australis Hector refined at 1.3\u00c5 resolution","volume":"238","author":"Housset","year":"1994","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib22","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1006\/jmbi.1993.1648","article-title":"Shape complementarily at protein\/protein interfaces","volume":"234","author":"Lawrence","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2004.06.067_bib23","doi-asserted-by":"crossref","first-page":"24125","DOI":"10.1074\/jbc.M211931200","article-title":"Importance of the conserved aromatic residues in the scorpion alpha-like toxin BmK M1: the hydrophobic surface region revisited","volume":"278","author":"Sun","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2004.06.067_bib24","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/0041-0101(86)90199-6","article-title":"Approximate LD50 determination of snake venoms using eight to ten animals","volume":"24","author":"Meier","year":"1986","journal-title":"Toxicon"},{"key":"10.1016\/j.jmb.2004.06.067_bib25","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1002\/pro.5560031206","article-title":"A revised set of potentials for \u03b2-turn formation in proteins","volume":"3","author":"Hutchinson","year":"1994","journal-title":"Protein Sci."},{"key":"10.1016\/j.jmb.2004.06.067_bib26","doi-asserted-by":"crossref","first-page":"15950","DOI":"10.1074\/jbc.271.27.15950","article-title":"Molecular determinants of high affinity binding of \u03b1-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the sodium channel \u03b1 subunit","volume":"271","author":"Rogers","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2004.06.067_bib27","doi-asserted-by":"crossref","first-page":"131","DOI":"10.3109\/15569549809009247","article-title":"Functional anatomy of scorpion toxins, affecting sodium channels","volume":"17","author":"Gordon","year":"1998","journal-title":"Toxicol. Toxin Rev."},{"key":"10.1016\/j.jmb.2004.06.067_bib28","doi-asserted-by":"crossref","first-page":"4699","DOI":"10.1021\/bi0270438","article-title":"Exploration of the functional site of a scorpion \u03b1-like toxin by site-directed mutagenesis","volume":"42","author":"Wang","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2004.06.067_bib29","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2174\/0929866023408562","article-title":"Purification and crystallization of a new alpha-like toxin with cardiac toxicity from scorpion Buthus martensii Karsch","volume":"9","author":"Guan","year":"2002","journal-title":"Protein Pept. Letters"},{"key":"10.1016\/j.jmb.2004.06.067_bib30","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1006\/prep.1999.1127","article-title":"Expression and purification of the BmK M1 neurotoxin from the scorpion Buthus martensii Karsch","volume":"17","author":"Shao","year":"1999","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jmb.2004.06.067_bib31","unstructured":"Nielsen, C., Arvai, A., Szebenyi, D. M. E., Deacon, A., Thiel, D. J., Bolotovsky, R., Van Zandt, K. C. & Rossmann, M. (1998). DPS: a data processing system for oscillation method data from a 2\u00d72 mosaic CCD detector. Abstract 11.06.06, ACA Meeting July 18\u201323."},{"key":"10.1016\/j.jmb.2004.06.067_bib32","unstructured":"Leslie, A. G. W. (1990). Crystallographic Computing: Oxford University Press."},{"key":"10.1016\/j.jmb.2004.06.067_bib33","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: programs for protein crystallography","volume":"50","year":"1994","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1016\/j.jmb.2004.06.067_bib34","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jmb.2004.06.067_bib35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1107\/S0108767393007597","article-title":"AMoRe\u2014an automated package for molecular replacement","volume":"50","author":"Navaza","year":"1994","journal-title":"Acta Crystallog. sect. A"},{"key":"10.1016\/j.jmb.2004.06.067_bib36","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: a new software suite for macromolecular structure determination","volume":"54","author":"Br\u00fcnger","year":"1998","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1016\/j.jmb.2004.06.067_bib37","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1107\/S0108767390010224","article-title":"Improved methods for binding protein models in electron density maps and the location of errors in these models","volume":"47","author":"Jones","year":"1991","journal-title":"Acta Crystallog. sect. A"},{"key":"10.1016\/j.jmb.2004.06.067_bib38","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1107\/S0907444996012255","article-title":"Refinement of macromolecular structures by the maximum-likelihood method","volume":"53","author":"Murshudov","year":"1997","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1016\/j.jmb.2004.06.067_bib39","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1046\/j.1432-1327.1999.00625.x","article-title":"Scorpion toxins specific for Na+-channels","volume":"264","author":"Possani","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jmb.2004.06.067_bib40","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.jmb.2004.06.067_bib41","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/protein\/6.1.37","article-title":"ALSCRIPT: a tool to format multiple sequence alignments","volume":"6","author":"Barton","year":"1993","journal-title":"Protein Eng."},{"key":"10.1016\/j.jmb.2004.06.067_bib42","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1107\/S0021889891004399","article-title":"MOLSCRIPT: a program to produce both detailed and schematic plots for protein structures","volume":"24","author":"Kraulis","year":"1991","journal-title":"J. Appl. Crystallog."},{"key":"10.1016\/j.jmb.2004.06.067_bib43","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1107\/S0907444994006396","article-title":"Raster3D version 2.0: a program for potorealistic molecular graphics","volume":"50","author":"Merritt","year":"1994","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1016\/j.jmb.2004.06.067_bib44","series-title":"TURBO-FRODO","author":"Roussel","year":"1992"}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283604007727?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283604007727?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T22:26:09Z","timestamp":1549405569000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283604007727"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["S0022283604007727"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2004.06.067","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2004,8]]}}}