{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:30:17Z","timestamp":1791307817211,"version":"4.3.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2005,7]]},"DOI":"10.1016\/j.jmb.2005.05.020","type":"journal-article","created":{"date-parts":[[2005,6,5]],"date-time":"2005-06-05T07:14:55Z","timestamp":1117955695000},"page":"735-743","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":95,"title":["Crystal Structure of a CRISP Family Ca2+-channel Blocker Derived from Snake Venom"],"prefix":"10.1016","volume":"350","author":[{"given":"Yasuo","family":"Shikamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Suto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mizuno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2005.05.020_bib1","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1073\/pnas.78.3.1675","article-title":"Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats","volume":"78","author":"Kierszenbaum","year":"1981","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.05.020_bib2","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1210\/mend-2-10-999","article-title":"Molecular cloning of complementary deoxyribonucleic acid for an androgen-regulated epididymal protein: sequence homology with metalloproteins","volume":"2","author":"Charest","year":"1988","journal-title":"Mol. Endocrinol."},{"key":"10.1016\/j.jmb.2005.05.020_bib3","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1095\/biolreprod67.4.1225","article-title":"Expression and structure\u2013function analysis of de, a sperm cysteine-rich secretory protein that mediates gamete fusion","volume":"67","author":"Ellerman","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.jmb.2005.05.020_bib4","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0888-7543(89)90019-0","article-title":"Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene. A testis-specific gene Tpx-1 maps between Pgk-2 and Mep-1 on mouse chromosome 17","volume":"5","author":"Kasahara","year":"1989","journal-title":"Genomics"},{"key":"10.1016\/j.jmb.2005.05.020_bib5","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/0014-5793(96)00030-0","article-title":"SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein","volume":"380","author":"Kjeldsen","year":"1996","journal-title":"FEBS Letters"},{"key":"10.1016\/j.jmb.2005.05.020_bib6","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0041-0101(90)90065-F","article-title":"Isolation and characterization of helothermine, a novel toxin from Heloderma horridum horridum (Mexican beaded lizard) venom","volume":"28","author":"Mochca-Morales","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.jmb.2005.05.020_bib7","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1016\/S0006-3495(95)80410-8","article-title":"Primary structure and properties of helothermine, a peptide toxin that blocks ryanodine receptors","volume":"68","author":"Morrissette","year":"1995","journal-title":"Biophys. J."},{"key":"10.1016\/j.jmb.2005.05.020_bib8","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/BF00232674","article-title":"The toxin helothermine affects potassium currents in newborn rat cerebellar granule cells","volume":"139","author":"Nobile","year":"1994","journal-title":"J. Membr. Biol."},{"key":"10.1016\/j.jmb.2005.05.020_bib9","first-page":"15","article-title":"Helothermine, a lizard venom toxin, inhibits calcium current in cerebellar granules","volume":"110","author":"Nobile","year":"1996","journal-title":"Expt. Brain Res."},{"key":"10.1016\/j.jmb.2005.05.020_bib10","doi-asserted-by":"crossref","first-page":"11205","DOI":"10.1073\/pnas.211316798","article-title":"Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins","volume":"98","author":"Olson","year":"2001","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.05.020_bib11","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S0960-9822(03)00108-8","article-title":"Identification of a DAF-16 transcriptional target gene, scl-1, that regulates longevity and stress resistance in Caenorhabditis elegans","volume":"13","author":"Ookuma","year":"2003","journal-title":"Curr. Biol."},{"key":"10.1016\/j.jmb.2005.05.020_bib12","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1006\/jmbi.1996.0772","article-title":"NMR solution structure of the pathogenesis-related protein P14a","volume":"266","author":"Fernandez","year":"1997","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.05.020_bib13","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/prot.1160","article-title":"Major venom allergen of yellow jackets. Ves v 5: structural characterization of a pathogenesis-related protein superfamily","volume":"45","author":"Henriksen","year":"2001","journal-title":"Proteins: Struct. Funct. Genet."},{"key":"10.1016\/j.jmb.2005.05.020_bib14","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.toxicon.2004.05.023","article-title":"Structure and function of snake venom cysteine-rich secretory proteins","volume":"44","author":"Yamazaki","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.jmb.2005.05.020_bib15","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0003-9861(03)00028-6","article-title":"Wide distribution of cysteine-rich secretory proteins in snake venoms: isolation and cloning of novel snake venom cysteine-rich secretory proteins","volume":"412","author":"Yamazaki","year":"2003","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.jmb.2005.05.020_bib16","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1046\/j.1432-1033.2002.02940.x","article-title":"Cloning and characterization of novel snake venom proteins that block smooth muscle contraction","volume":"269","author":"Yamazaki","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jmb.2005.05.020_bib17","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1073\/pnas.96.2.754","article-title":"Pseudechetoxin: a peptide blocker of cyclic nucleotide-gated ion channels","volume":"96","author":"Brown","year":"1999","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.05.020_bib18","doi-asserted-by":"crossref","first-page":"11331","DOI":"10.1021\/bi026132h","article-title":"Purification and cloning of toxins from elapid venoms that target cyclic nucleotide-gated ion channels","volume":"41","author":"Yamazaki","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.05.020_bib19","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1085\/jgp.200308823","article-title":"Pseudechetoxin binds to the pore turret of cyclic nucleotide-gated ion channels","volume":"122","author":"Brown","year":"2003","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.jmb.2005.05.020_bib20","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1006\/jmbi.2000.3609","article-title":"Solution structure of hanatoxin1, a gating modifier of voltage-dependent K(+) channels: common surface features of gating modifier toxins","volume":"297","author":"Takahashi","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.05.020_bib21","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1021\/bi0353373","article-title":"Solution structure and functional characterization of SGTx1, a modifier of Kv2.1 channel gating","volume":"43","author":"Lee","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.05.020_bib22","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nature02632","article-title":"A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom","volume":"430","author":"Lee","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2005.05.020_bib23","doi-asserted-by":"crossref","first-page":"6739","DOI":"10.1021\/bi00188a001","article-title":"Structural mechanisms for domain movements in proteins","volume":"33","author":"Gerstein","year":"1994","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.05.020_bib24","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1073\/pnas.95.5.2262","article-title":"Structure comparison of human glioma pathogenesis-related protein GliPR and the plant pathogenesis-related protein P14a indicates a functional link between the human immune system and a plant defense system","volume":"95","author":"Szyperski","year":"1998","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.05.020_bib25","doi-asserted-by":"crossref","first-page":"31105","DOI":"10.1074\/jbc.M304843200","article-title":"Isolation and characterization of a cone snail protease with homology to CRISP proteins of the pathogenesis-related protein superfamily","volume":"278","author":"Milne","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2005.05.020_bib26","first-page":"H74","article-title":"Phenylephrine contracts rat tail artery by one electromechanical and three pharmacomechanical mechanisms","volume":"268","author":"Chen","year":"1995","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.jmb.2005.05.020_bib27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1146\/annurev.physiol.61.1.85","article-title":"Excitation-contraction coupling in gastrointestinal and other smooth muscles","volume":"61","author":"Bolton","year":"1999","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.jmb.2005.05.020_bib28","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1042\/bj20020191","article-title":"Membrane depolarization-induced contraction of rat caudal arterial smooth muscle involves Rho-associated kinase","volume":"364","author":"Mita","year":"2002","journal-title":"Biochem. J."},{"key":"10.1016\/j.jmb.2005.05.020_bib29","doi-asserted-by":"crossref","first-page":"8748","DOI":"10.1021\/bi9602674","article-title":"A novel hydrophobic omega-conotoxin blocks molluscan dihydropyridine-sensitive calcium channels","volume":"35","author":"Fainzilber","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.05.020_bib30","doi-asserted-by":"crossref","first-page":"12014","DOI":"10.1074\/jbc.272.18.12014","article-title":"Structure\u2013function relationships of omega-conotoxin GVIA. Synthesis, structure, calcium channel binding, and functional assay of alanine-substituted analogues","volume":"272","author":"Lew","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2005.05.020_bib31","doi-asserted-by":"crossref","first-page":"12405","DOI":"10.1074\/jbc.M413566200","article-title":"Crystal structure of the cysteine-rich secretory protein stecrisp reveals that the cysteine-rich domain has a K+ channel inhibitor-like fold","volume":"280","author":"Guo","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2005.05.020_bib32","doi-asserted-by":"crossref","first-page":"11963","DOI":"10.1021\/bi026400b","article-title":"Mutating a critical lysine in ShK toxin alters its binding configuration in the pore-vestibule region of the voltage-gated potassium channel, Kv1.3","volume":"41","author":"Lanigan","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.05.020_bib33","doi-asserted-by":"crossref","first-page":"21885","DOI":"10.1074\/jbc.274.31.21885","article-title":"Structural conservation of the pores of calcium-activated and voltage-gated potassium channels determined by a sea anemone toxin","volume":"274","author":"Rauer","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2005.05.020_bib34","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0022-2836(68)90205-2","article-title":"Solvent content of protein crystals","volume":"33","author":"Matthews","year":"1968","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.05.020_bib35","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: programs for protein crystallography","volume":"50","year":"1994","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1016\/j.jmb.2005.05.020_bib36","series-title":"Methods Enzymology","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/S0076-6879(97)76073-7","article-title":"Maximum-likelihood heavy-atom parameter refinement for the multiple isomorphous replacement and multiwavelength anomalous diffraction methods","author":"de La Fortelle","year":"1997"},{"key":"10.1016\/j.jmb.2005.05.020_bib37","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography and NMR system: a new software suite for macromolecular structure determination","volume":"54","author":"Brunger","year":"1998","journal-title":"Acta Crystallog. sect. D"}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283605005516?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283605005516?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T06:04:18Z","timestamp":1762668258000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283605005516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["S0022283605005516"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2005.05.020","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2005,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Crystal Structure of a CRISP Family Ca2+-channel Blocker Derived from Snake Venom","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2005.05.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2005 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}