{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:56:17Z","timestamp":1790006177678,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM55767"],"award-info":[{"award-number":["GM55767"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI064611"],"award-info":[{"award-number":["AI064611"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI55882"],"award-info":[{"award-number":["AI55882"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 GM07283"],"award-info":[{"award-number":["T32 GM07283"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2005,10]]},"DOI":"10.1016\/j.jmb.2005.08.041","type":"journal-article","created":{"date-parts":[[2005,9,7]],"date-time":"2005-09-07T15:11:20Z","timestamp":1126105880000},"page":"308-321","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":45,"title":["Characterization of T Cell Receptors Engineered for High Affinity Against Toxic Shock Syndrome Toxin-1"],"prefix":"10.1016","volume":"353","author":[{"given":"Rebecca A.","family":"Buonpane","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beenu","family":"Moza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Sundberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Kranz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2005.08.041_bib1","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/S0140-6736(78)92274-2","article-title":"Toxic-shock syndrome associated with phage-group-I Staphylococci","volume":"2","author":"Todd","year":"1978","journal-title":"Lancet"},{"key":"10.1016\/j.jmb.2005.08.041_bib2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/infdis\/143.4.509","article-title":"Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome","volume":"143","author":"Schlievert","year":"1981","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jmb.2005.08.041_bib3","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/S0140-6736(81)92186-3","article-title":"A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates","volume":"1","author":"Bergdoll","year":"1981","journal-title":"Lancet"},{"key":"10.1016\/j.jmb.2005.08.041_bib4","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1146\/annurev.micro.55.1.77","article-title":"Toxic shock syndrome and bacterial superantigens: an update","volume":"55","author":"McCormick","year":"2001","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.jmb.2005.08.041_bib5","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1126\/science.2524876","article-title":"V beta-specific stimulation of human T cells by staphylococcal toxins","volume":"244","author":"Kappler","year":"1989","journal-title":"Science"},{"key":"10.1016\/j.jmb.2005.08.041_bib6","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1126\/science.2185544","article-title":"The staphylococcal entertoxins and their relatives","volume":"248","author":"Marrack","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.jmb.2005.08.041_bib7","doi-asserted-by":"crossref","first-page":"13761","DOI":"10.1021\/bi00213a001","article-title":"Structure of toxic shock syndrome toxin 1","volume":"32","author":"Prasad","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2005.08.041_bib8","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/367094a0","article-title":"Structural basis of superantigen action inferred from crystal structure of toxic-shock syndrome toxin-1","volume":"367","author":"Acharya","year":"1994","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2005.08.041_bib9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S0952-7915(01)00296-5","article-title":"So many ways of getting in the way: diversity in the molecular architecture of superantigen-dependent T-cell signaling complexes","volume":"14","author":"Sundberg","year":"2002","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.jmb.2005.08.041_bib10","doi-asserted-by":"crossref","first-page":"8941","DOI":"10.1073\/pnas.86.22.8941","article-title":"Interaction of Staphylococcus aureus toxin \u201csuperantigens\u201d with human T cells","volume":"86","author":"Choi","year":"1989","journal-title":"Proc. Natl Acad. Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.08.041_bib11","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1084\/jem.172.3.981","article-title":"Selective expansion of T cells expressing V beta 2 in toxic shock syndrome","volume":"172","author":"Choi","year":"1990","journal-title":"J. Exp. Med"},{"key":"10.1016\/j.jmb.2005.08.041_bib12","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0969-2126(02)00759-1","article-title":"Structures of two streptococcal superantigens bound to TCR beta chains reveal diversity in the architecture of T cell signaling complexes","volume":"10","author":"Sundberg","year":"2002","journal-title":"Structure (Camb)"},{"key":"10.1016\/j.jmb.2005.08.041_bib13","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.4049\/jimmunol.171.3.1385","article-title":"Functional analysis of the TCR binding domain of toxic shock syndrome toxin-1 predicts further diversity in MHC class II\/superantigen\/TCR ternary complexes","volume":"171","author":"McCormick","year":"2003","journal-title":"J. Immunol."},{"key":"10.1016\/j.jmb.2005.08.041_bib14","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1038\/nbt0697-553","article-title":"Yeast surface display for screening combinatorial polypeptide libraries","volume":"15","author":"Boder","year":"1997","journal-title":"Nature Biotech."},{"key":"10.1016\/j.jmb.2005.08.041_bib15","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1006\/jmbi.2001.4560","article-title":"High affinity T cell receptors from yeast display libraries block T cell activation by superantigens","volume":"307","author":"Kieke","year":"2001","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.08.041_bib16","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.1073\/pnas.96.10.5651","article-title":"Selection of functional T cell receptor mutants from a yeast surface- display library","volume":"96","author":"Kieke","year":"1999","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.08.041_bib17","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/77325","article-title":"Directed evolution of a stable scaffold for T-cell receptor engineering","volume":"18","author":"Shusta","year":"2000","journal-title":"Nature Biotechnol."},{"key":"10.1016\/j.jmb.2005.08.041_bib18","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1006\/jmbi.1999.3130","article-title":"Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency","volume":"292","author":"Shusta","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.08.041_bib19","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1126\/science.7701320","article-title":"Crystal structure of the \u03b2 chain of a T cell antigen receptor","volume":"267","author":"Bentley","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.jmb.2005.08.041_bib20","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1126\/science.274.5285.209","article-title":"An \u03b1\u03b2 T cell receptor structure at 2.5 angstrom and its orientation in the TCR-MHC complex","volume":"274","author":"Garcia","year":"1996","journal-title":"Science"},{"key":"10.1016\/j.jmb.2005.08.041_bib21","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1084\/jem.182.6.1833","article-title":"Superantigen binding to a T cell receptor \u03b2 chain of known three-dimensional structure","volume":"182","author":"Malchiodi","year":"1995","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jmb.2005.08.041_bib22","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1038\/384188a0","article-title":"Crystal structure of a T-cell receptor \u03b2-chain complexed with a superantigen","volume":"384","author":"Fields","year":"1996","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2005.08.041_bib23","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S1074-7613(00)80646-9","article-title":"Three-dimensional structure of the complex between a T cell receptor beta chain and the superantigen staphylococcal enterotoxin B","volume":"9","author":"Li","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.jmb.2005.08.041_bib24","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.immunol.17.1.435","article-title":"The structural basis of T cell activation by superantigens","volume":"17","author":"Li","year":"1999","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.jmb.2005.08.041_bib25","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1021\/bp970144q","article-title":"Optimal screening of surface-displayed polypeptide libraries","volume":"14","author":"Boder","year":"1998","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jmb.2005.08.041_bib26","doi-asserted-by":"crossref","first-page":"10701","DOI":"10.1073\/pnas.170297297","article-title":"Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity","volume":"97","author":"Boder","year":"2000","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.08.041_bib27","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jmb.2004.11.057","article-title":"High-affinity, peptide-specific T cell receptors can be generated by mutations in CDR1, CDR2 or CDR3","volume":"346","author":"Chlewicki","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.08.041_bib28","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.jmb.2004.07.053","article-title":"Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display","volume":"342","author":"Chao","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2005.08.041_bib29","first-page":"455","article-title":"Human T-cell receptor variable gene segment families","volume":"42","author":"Arden","year":"1995","journal-title":"Immunogenetics"},{"key":"10.1016\/j.jmb.2005.08.041_bib30","doi-asserted-by":"crossref","first-page":"50412","DOI":"10.1074\/jbc.M306848200","article-title":"Dissecting cooperative and additive binding energetics in the affinity maturation pathway of a protein-protein interface","volume":"278","author":"Yang","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2005.08.041_bib31","first-page":"501","article-title":"Mouse T-cell receptor variable gene segment families","volume":"42","author":"Arden","year":"1995","journal-title":"Immunogenetics"},{"key":"10.1016\/j.jmb.2005.08.041_bib32","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/nrd1694","article-title":"The evolving field of biodefence: therapeutic developments and diagnostics","volume":"4","author":"Burnett","year":"2005","journal-title":"Nature Rev. Drug Discov."},{"key":"10.1016\/j.jmb.2005.08.041_bib33","doi-asserted-by":"crossref","first-page":"5387","DOI":"10.1073\/pnas.080078297","article-title":"In vitro evolution of a T cell receptor with high affinity for peptide\/MHC","volume":"97","author":"Holler","year":"2000","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2005.08.041_bib34","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2144\/99261rr02","article-title":"General method for plasmid construction using homologous recombination","volume":"26","author":"Raymond","year":"1999","journal-title":"Biotechniques"},{"key":"10.1016\/j.jmb.2005.08.041_bib35","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/proeng\/gzg018","article-title":"Directed evolution of a single-chain class II MHC product by yeast display","volume":"16","author":"Starwalt","year":"2003","journal-title":"Protein Eng."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283605009782?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283605009782?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:24:10Z","timestamp":1777418650000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283605009782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["S0022283605009782"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2005.08.041","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2005,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of T Cell Receptors Engineered for High Affinity Against Toxic Shock Syndrome Toxin-1","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2005.08.041","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2005 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}