{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:55:01Z","timestamp":1790304901133,"version":"4.1.0"},"reference-count":55,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2006,8]]},"DOI":"10.1016\/j.jmb.2006.06.028","type":"journal-article","created":{"date-parts":[[2006,7,4]],"date-time":"2006-07-04T11:13:10Z","timestamp":1152011590000},"page":"336-351","source":"Crossref","is-referenced-by-count":318,"title":["Location of High and Low Affinity Fatty Acid Binding Sites on Human Serum Albumin Revealed by NMR Drug-competition Analysis"],"prefix":"10.1016","volume":"361","author":[{"given":"Jeffrey R.","family":"Simard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Zunszain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Curry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2006.06.028_bib1","first-page":"152","article-title":"Structure of serum albumin","volume":"45","author":"Carter","year":"1994","journal-title":"Advan. Protein. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib2","series-title":"All About Albumin: Biochemistry, Genetics and Medical Applications","author":"Peters","year":"1995"},{"key":"10.1016\/j.jmb.2006.06.028_bib3","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1432-1033.1990.tb15495.x","article-title":"Multiple fatty acid binding to albumin in human blood plasma","volume":"189","author":"Brodersen","year":"1990","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jmb.2006.06.028_bib4","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/0076-6879(86)28077-5","article-title":"Structure and lipid binding properties of serum albumin","volume":"128","author":"Spector","year":"1986","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jmb.2006.06.028_bib5","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1748-1716.1991.tb09205.x","article-title":"Effect of exercise on recovery changes in plasma levels of FFA, glycerol, glucose and catecholamines","volume":"143","author":"Bahr","year":"1991","journal-title":"Acta Physiol. Scand."},{"key":"10.1016\/j.jmb.2006.06.028_bib6","first-page":"E382","article-title":"Role of triglyceride-fatty acid cycle in controlling fat metabolism in humans during and after exercise","volume":"258","author":"Wolfe","year":"1990","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.jmb.2006.06.028_bib7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1123\/ijsn.6.4.337","article-title":"Effects of active warm-down and carbohydrate feeding on free fatty acid concentrations after prolonged submaximal exercise","volume":"6","author":"El-Sayed","year":"1996","journal-title":"Int. J. Sport. Nutr."},{"key":"10.1016\/j.jmb.2006.06.028_bib8","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s001250051130","article-title":"Fatty acids, lipotoxicity and insulin secretion","volume":"42","author":"McGarry","year":"1999","journal-title":"Diabetologia"},{"key":"10.1016\/j.jmb.2006.06.028_bib9","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1007\/BF00422371","article-title":"A high concentration of fasting plasma non-esterified fatty acids is a risk factor for the development of NIDDM","volume":"38","author":"Paolisso","year":"1995","journal-title":"Diabetologia"},{"key":"10.1016\/j.jmb.2006.06.028_bib10","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0033-0620(71)80012-9","article-title":"Free fatty acids during acute myocardial infarction","volume":"13","author":"Kurien","year":"1971","journal-title":"Prog. Cardiovasc. Dis."},{"key":"10.1016\/j.jmb.2006.06.028_bib11","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0928-0987(01)00219-6","article-title":"Evaluation of the human serum albumin column as a discovery screening tool for plasma protein binding","volume":"15","author":"Buchholz","year":"2002","journal-title":"Eur. J. Pharm. Sci."},{"key":"10.1016\/j.jmb.2006.06.028_bib12","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/90761","article-title":"ADMET - turning chemicals into drugs","volume":"19","author":"Hodgson","year":"2001","journal-title":"Nature Biotechnol."},{"key":"10.1016\/j.jmb.2006.06.028_bib13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1006\/abio.2001.5314","article-title":"High-resolution and high-throughput protocols for measuring drug\/human serum albumin interactions using BIOCORE","volume":"296","author":"Rich","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jmb.2006.06.028_bib14","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1002\/jps.10494","article-title":"Fast gradient HPLC method to determine compounds binding to human serum albumin. Relationships with octanol\/water and immobilized artificial membrane lipophilicity","volume":"92","author":"Valko","year":"2003","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.jmb.2006.06.028_bib15","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/S0006-2952(02)01074-2","article-title":"Predicting plasma protein binding of drugs: a new approach","volume":"64","author":"Kratochwil","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.jmb.2006.06.028_bib16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1067\/mcp.2002.121829","article-title":"Changes in plasma protein binding have little clinical relevance","volume":"71","author":"Benet","year":"2002","journal-title":"Clin. Pharmacol. Ther."},{"key":"10.1016\/j.jmb.2006.06.028_bib17","doi-asserted-by":"crossref","first-page":"44","DOI":"10.2165\/00003088-199426010-00004","article-title":"Drug binding in plasma: A summary of recent trends in the study of drug and hormone binding","volume":"26","author":"Herve","year":"1994","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.jmb.2006.06.028_bib18","series-title":"Pharmacology: Drug Actions and Reactions","author":"Levine","year":"2000"},{"key":"10.1016\/j.jmb.2006.06.028_bib19","series-title":"Principles of Drug Action: The Basis of Pharmacology","author":"Pratt","year":"1990"},{"key":"10.1016\/j.jmb.2006.06.028_bib20","first-page":"1052","article-title":"Further characterization of specific drug binding sites on human serum albumin","volume":"12","author":"Sudlow","year":"1976","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.jmb.2006.06.028_bib21","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jmb.2005.07.075","article-title":"Structural basis of the drug-binding specificity of human serum albumin","volume":"353","author":"Ghuman","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2006.06.028_bib22","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/protein\/12.6.439","article-title":"Crystal structure of human serum albumin at 2.5\u00a0\u00c5 resolution","volume":"12","author":"Sugio","year":"1999","journal-title":"Protein Eng."},{"key":"10.1016\/j.jmb.2006.06.028_bib23","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/1869","article-title":"Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites","volume":"5","author":"Curry","year":"1998","journal-title":"Nature Struct. Biol."},{"key":"10.1016\/j.jmb.2006.06.028_bib24","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/358209a0","article-title":"Atomic structure and chemistry of human serum albumin","volume":"358","author":"He","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2006.06.028_bib25","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1006\/jmbi.2000.4158","article-title":"Crystallographic analysis reveals common modes of binding of medium and long-chain fatty acids to human serum albumin","volume":"303","author":"Bhattacharya","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2006.06.028_bib26","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S1388-1981(99)00148-1","article-title":"Fatty acid binding to human serum albumin: new insights from crystallographic studies","volume":"1441","author":"Curry","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jmb.2006.06.028_bib27","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1006\/jmbi.2000.5208","article-title":"Crystal structures of human serum albumin complexed with monounsaturated and polyunsaturated fatty acids","volume":"314","author":"Petitpas","year":"2001","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2006.06.028_bib28","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1006\/bbrc.2002.6540","article-title":"The atomic structure of human methemalbumin at 1.9\u00a0\u00c5","volume":"291","author":"Wardell","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jmb.2006.06.028_bib29","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1472-6807-3-6","article-title":"Crystal structural analysis of human serum albumin complexed with hemin and fatty acid","volume":"3","author":"Zunszain","year":"2003","journal-title":"BMC Struct. Biol."},{"key":"10.1016\/j.jmb.2006.06.028_bib30","doi-asserted-by":"crossref","first-page":"6440","DOI":"10.1073\/pnas.1137188100","article-title":"Structural basis of albumin-thyroxine interactions and familial dysalbuminemic hyperthyroxinemia","volume":"100","author":"Petitpas","year":"2003","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2006.06.028_bib31","doi-asserted-by":"crossref","first-page":"38731","DOI":"10.1074\/jbc.M005460200","article-title":"Binding of the general anesthetics propofol and halothane to human serum albumin: high-resolution crystal structures","volume":"275","author":"Bhattacharya","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib32","doi-asserted-by":"crossref","first-page":"22804","DOI":"10.1074\/jbc.M100575200","article-title":"Crystal structure analysis of warfarin binding to human serum albumin: anatomy of drug site I","volume":"276","author":"Petitpas","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib33","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1002\/prot.10068","article-title":"Probing the structure of the warfarin-binding site on human serum albumin using site-directed mutagenesis","volume":"47","author":"Petersen","year":"2002","journal-title":"Proteins: Struct. Funct. Genet."},{"key":"10.1016\/j.jmb.2006.06.028_bib34","doi-asserted-by":"crossref","first-page":"10971","DOI":"10.1016\/S0021-9258(18)60913-5","article-title":"Carbon 13 NMR studies of saturated fatty acids bound to bovine serum albumin: I. The filling of individual fatty acid binding sites","volume":"262","author":"Cistola","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib35","doi-asserted-by":"crossref","first-page":"3718","DOI":"10.1073\/pnas.81.12.3718","article-title":"Interactions of myristic acid with bovine serum albumin: A 13C NMR study","volume":"81","author":"Hamilton","year":"1984","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2006.06.028_bib36","doi-asserted-by":"crossref","first-page":"9262","DOI":"10.1016\/S0021-9258(17)44661-8","article-title":"Interactions of the carboxyl group of oleic acid with bovine serum albumin: A 13C NMR study","volume":"258","author":"Parks","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib37","doi-asserted-by":"crossref","first-page":"10980","DOI":"10.1016\/S0021-9258(18)60914-7","article-title":"Carbon 13 NMR studies of saturated fatty acids bound to bovine serum albumin: II. Electrostatic interactions in individual fatty acid binding sites","volume":"262","author":"Cistola","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2006.06.028_bib38","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1073\/pnas.88.6.2051","article-title":"Locations of the three primary binding sites for long-chain fatty acids on bovine serum albumin","volume":"88","author":"Hamilton","year":"1991","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2006.06.028_bib39","doi-asserted-by":"crossref","first-page":"17958","DOI":"10.1073\/pnas.0506440102","article-title":"Locating high-affinity fatty acid-binding sites on albumin by x-ray crystallography and NMR spectroscopy","volume":"102","author":"Simard","year":"2005","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2006.06.028_bib40","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.bbrc.2005.06.127","article-title":"Heme impairs allosterically drug binding to human serum albumin Sudlow's site I","volume":"334","author":"Ascenzi","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jmb.2006.06.028_bib41","doi-asserted-by":"crossref","first-page":"4672","DOI":"10.1111\/j.1742-4658.2005.04883.x","article-title":"Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin. Optical and NMR spectroscopy characterization","volume":"272","author":"Fanali","year":"2005","journal-title":"FEBS J."},{"key":"10.1016\/j.jmb.2006.06.028_bib42","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1194\/jlr.M200041-JLR200","article-title":"Interactions of very long chain fatty acids with serum albumin","volume":"43","author":"Choi","year":"2002","journal-title":"J. Lipid. Res."},{"key":"10.1016\/j.jmb.2006.06.028_bib43","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1073\/pnas.86.8.2663","article-title":"Medium-chain fatty acid binding to albumin and transfer to phospholipid bilayers","volume":"86","author":"Hamilton","year":"1989","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2006.06.028_bib44","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1172\/JCI118182","article-title":"Interactions of a very long chain fatty acid with model membranes and serum albumin","volume":"96","author":"Ho","year":"1995","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jmb.2006.06.028_bib45","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0022-2275(20)36723-7","article-title":"Fatty acid binding to plasma albumin","volume":"16","author":"Spector","year":"1975","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jmb.2006.06.028_bib46","first-page":"421","article-title":"The binding sites on human serum albumin for some nonsteroidal antiinflammatory drugs","volume":"18","author":"Kober","year":"1980","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.jmb.2006.06.028_bib47","first-page":"767","article-title":"Binding of dugs to human serum albumin: XI. The specificity of three binding sites as studied with albumin immobilized in microparticles","volume":"16","author":"Sj\u00f6holm","year":"1979","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.jmb.2006.06.028_bib48","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0009-2797(95)03613-Q","article-title":"Binding of coumarins to site I of human serum albumin. Effect of the fatty acids","volume":"97","author":"Zat\u00f3n","year":"1995","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.jmb.2006.06.028_bib49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0009-2797(99)00130-1","article-title":"Study of heterocycle rings binding to human serum albumin","volume":"124","author":"Zat\u00f3n","year":"2000","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.jmb.2006.06.028_bib50","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1054\/plef.2002.0400","article-title":"How fatty acids bind to proteins: the inside story from protein structures","volume":"67","author":"Hamilton","year":"2002","journal-title":"Prostaglandins Leukot. Essent. Fatty Acids"},{"key":"10.1016\/j.jmb.2006.06.028_bib51","doi-asserted-by":"crossref","first-page":"7574","DOI":"10.1021\/bi00080a032","article-title":"Interactions of long-chain fatty acids and albumin: Determination of free fatty acid levels using the fluorescent probe ADIFAB","volume":"32","author":"Richieri","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2006.06.028_bib52","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1159\/000137726","article-title":"Effect of in vivo increase of free fatty acids on human plasma protein binding of furosemide","volume":"25","author":"Colombo","year":"1982","journal-title":"Pharmacology"},{"key":"10.1016\/j.jmb.2006.06.028_bib53","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/clpt.1984.241","article-title":"Abnormal serum protein binding of acidic drugs in diabetes mellitus","volume":"36","author":"Ruiz-Cabello","year":"1984","journal-title":"Clin. Pharmacol. Ther."},{"key":"10.1016\/j.jmb.2006.06.028_bib54","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/BF02280763","article-title":"Drug-protein binding kinetics in patients with type I diabetes","volume":"43","author":"Worner","year":"1992","journal-title":"Eur. J. Clin. Pharmacol."},{"key":"10.1016\/j.jmb.2006.06.028_bib55","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ab.2005.09.039","article-title":"Determining molecular binding sites on human serum albumin by displacement of oleic acid","volume":"347","author":"Sarver","year":"2005","journal-title":"Anal. Biochem."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283606007509?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283606007509?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T04:53:01Z","timestamp":1627534381000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283606007509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["S0022283606007509"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2006.06.028","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2006,8]]}}}