{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T19:54:00Z","timestamp":1782762840928,"version":"3.54.5"},"reference-count":31,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006264","name":"RIKEN","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006264","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2009,10]]},"DOI":"10.1016\/j.jmb.2009.07.054","type":"journal-article","created":{"date-parts":[[2009,7,24]],"date-time":"2009-07-24T04:51:37Z","timestamp":1248411097000},"page":"937-951","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Crystal Structure of the \u03b1 Subunit of Human Translation Initiation Factor 2B"],"prefix":"10.1016","volume":"392","author":[{"given":"Takuya B.","family":"Hiyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuhiro","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Imataka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeyuki","family":"Yokoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2009.07.054_bib1","series-title":"Translational Control in Biology and Medicine","first-page":"87","article-title":"The mechanism of translation initiation in eukaryotes","author":"Pestova","year":"2007"},{"key":"10.1016\/j.jmb.2009.07.054_bib2","series-title":"Translational Control in Biology and Medicine","first-page":"225","article-title":"The mechanism of translation initiation in the yeast Saccharomyces cerevisiae","author":"Hinnebusch","year":"2007"},{"key":"10.1016\/j.jmb.2009.07.054_bib3","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1016\/S0021-9258(18)60596-4","article-title":"The catalytic mechanism of guanine nucleotide exchange factor action and competitive inhibition by phosphorylated eukaryotic initiation factor 2","volume":"263","author":"Rowlands","year":"1988","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.07.054_bib4","doi-asserted-by":"crossref","first-page":"5018","DOI":"10.1128\/MCB.21.15.5018-5030.2001","article-title":"Tight binding of the phosphorylated alpha subunit of initiation factor 2 (eIF2alpha) to the regulatory subunits of guanine nucleotide exchange factor eIF2B is required for inhibition of translation initiation","volume":"21","author":"Krishnamoorthy","year":"2001","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib5","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1101\/gad.12.4.514","article-title":"eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange","volume":"12","author":"Pavitt","year":"1998","journal-title":"Genes Dev."},{"key":"10.1016\/j.jmb.2009.07.054_bib6","doi-asserted-by":"crossref","first-page":"5292","DOI":"10.1093\/emboj\/cdf515","article-title":"Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation","volume":"21","author":"Gomez","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2009.07.054_bib7","doi-asserted-by":"crossref","first-page":"12359","DOI":"10.1074\/jbc.272.19.12359","article-title":"Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells","volume":"272","author":"Fabian","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.07.054_bib8","doi-asserted-by":"crossref","first-page":"12841","DOI":"10.1074\/jbc.273.21.12841","article-title":"Regulation of guanine nucleotide exchange through phosphorylation of eukaryotic initiation factor eIF2alpha. Role of the alpha- and delta-subunits of eiF2b","volume":"273","author":"Kimball","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.07.054_bib9","first-page":"3208","article-title":"Mutations in the GCD7 subunit of yeast guanine nucleotide exchange factor eIF-2B overcome the inhibitory effects of phosphorylated eIF-2 on translation initiation","volume":"14","author":"Vazquez de Aldana","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib10","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1128\/MCB.17.3.1298","article-title":"Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2","volume":"17","author":"Pavitt","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib11","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/ng764","article-title":"Subunits of the translation initiation factor eIF2B are mutant in leukoencephalopathy with vanishing white matter","volume":"29","author":"Leegwater","year":"2001","journal-title":"Nat. Genet."},{"key":"10.1016\/j.jmb.2009.07.054_bib12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1042\/BST0340022","article-title":"The large spectrum of eIF2B-related diseases","volume":"34","author":"Fogli","year":"2006","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.jmb.2009.07.054_bib13","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S1474-4422(06)70440-9","article-title":"Vanishing white matter disease","volume":"5","author":"van der Knaap","year":"2006","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.jmb.2009.07.054_bib14","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1073\/pnas.95.7.3726","article-title":"Archaeal translation initiation revisited: the initiation factor 2 and eukaryotic initiation factor 2B alpha\u2013beta\u2013delta subunit families","volume":"95","author":"Kyrpides","year":"1998","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.07.054_bib15","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.bbrc.2004.05.045","article-title":"Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B","volume":"319","author":"Kakuta","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jmb.2009.07.054_bib16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/jmbi.1993.1489","article-title":"Protein structure comparison by alignment of distance matrices","volume":"233","author":"Holm","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib17","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1110\/ps.073169008","article-title":"Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism","volume":"17","author":"Tamura","year":"2008","journal-title":"Protein Sci."},{"key":"10.1016\/j.jmb.2009.07.054_bib18","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jmb.2009.07.054_bib19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/mrdd.20104","article-title":"Vanishing white matter disease: a review with focus on its genetics","volume":"12","author":"Pronk","year":"2006","journal-title":"Ment. Retard. Dev. Disabil. Res. Rev."},{"key":"10.1016\/j.jmb.2009.07.054_bib20","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1093\/genetics\/126.3.549","article-title":"The translational activator GCN3 functions downstream from GCN1 and GCN2 in the regulatory pathway that couples GCN4 expression to amino acid availability in Saccharomyces cerevisiae","volume":"126","author":"Hannig","year":"1990","journal-title":"Genetics"},{"key":"10.1016\/j.jmb.2009.07.054_bib21","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1128\/MCB.16.11.6603","article-title":"Identification of a regulatory subcomplex in the guanine nucleotide exchange factor eIF2B that mediates inhibition by phosphorylated eIF2","volume":"16","author":"Yang","year":"1996","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib22","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: a new software suite for macromolecular structure determination","volume":"54","author":"Brunger","year":"1998","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.07.054_bib23","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/j.str.2004.07.010","article-title":"Solution structure of human initiation factor eIF2alpha reveals homology to the elongation factor eEF1B","volume":"12","author":"Ito","year":"2004","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2009.07.054_bib24","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.cell.2005.06.044","article-title":"Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR","volume":"122","author":"Dar","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2009.07.054_bib25","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1128\/MCB.25.8.3063-3075.2005","article-title":"PKR and GCN2 kinases and guanine nucleotide exchange factor eukaryotic translation initiation factor 2B (eIF2B) recognize overlapping surfaces on eIF2alpha","volume":"25","author":"Dey","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib26","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1016\/j.bbrc.2005.10.150","article-title":"Interaction of recombinant human eIF2 subunits with eIF2B and eIF2alpha kinases","volume":"338","author":"Suragani","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jmb.2009.07.054_bib27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jmb.2009.07.054_bib28","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0022-2836(68)90205-2","article-title":"Solvent content of protein crystals","volume":"33","author":"Matthews","year":"1968","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib29","first-page":"215","article-title":"Automated structure solution with autoSHARP","volume":"364","author":"Vonrhein","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jmb.2009.07.054_bib30","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.07.054_bib31","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1006\/jmbi.1993.1351","article-title":"Main-chain bond lengths and bond angles in protein structures","volume":"231","author":"Laskowski","year":"1993","journal-title":"J. Mol. Biol."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609009085?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609009085?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T21:07:48Z","timestamp":1758316068000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283609009085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["S0022283609009085"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2009.07.054","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2009,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Crystal Structure of the \u03b1 Subunit of Human Translation Initiation Factor 2B","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2009.07.054","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}