{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:25:31Z","timestamp":1791019531118,"version":"4.1.0"},"reference-count":42,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2010,1]]},"DOI":"10.1016\/j.jmb.2009.10.018","type":"journal-article","created":{"date-parts":[[2009,10,24]],"date-time":"2009-10-24T04:23:55Z","timestamp":1256358235000},"page":"89-104","source":"Crossref","is-referenced-by-count":43,"title":["DARPin-Assisted Crystallography of the CC2-LZ Domain of NEMO Reveals a Coupling between Dimerization and Ubiquitin Binding"],"prefix":"10.1016","volume":"395","author":[{"given":"Olivera","family":"Grubisha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Kaminska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Duquerroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Fontan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Cordier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Haouz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Raynal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne","family":"Chiaravalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Delepierre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Isra\u00ebl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"V\u00e9ron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Agou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2009.10.018_bib1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.cell.2008.01.020","article-title":"Shared principles in NF-kappaB signaling","volume":"132","author":"Hayden","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib2","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0092-8674(00)81466-X","article-title":"Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation","volume":"93","author":"Yamaoka","year":"1998","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib3","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/26261","article-title":"IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex","volume":"395","author":"Rothwarf","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.10.018_bib4","doi-asserted-by":"crossref","first-page":"54248","DOI":"10.1074\/jbc.M406423200","article-title":"Inhibition of NF-kappa B activation by peptides targeting NF-kappa B essential modulator (nemo) oligomerization","volume":"279","author":"Agou","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.018_bib5","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.str.2008.02.012","article-title":"Structure of a NEMO\/IKK-associating domain reveals architecture of the interaction site","volume":"16","author":"Rushe","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2009.10.018_bib6","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.molcel.2008.04.029","article-title":"Crystal structure of a vFlip\u2013IKKgamma complex: insights into viral activation of the IKK signalosome","volume":"30","author":"Bagneris","year":"2008","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib7","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1042\/BJ20090427","article-title":"NEMO oligomerization and its ubiquitin-binding properties","volume":"421","author":"Ivins","year":"2009","journal-title":"Biochem. J."},{"key":"10.1016\/j.jmb.2009.10.018_bib8","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.tcb.2006.09.004","article-title":"Posttranslational modifications of NEMO and its partners in NF-kappaB signaling","volume":"16","author":"Sebban","year":"2006","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/ncb1821","article-title":"Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation","volume":"11","author":"Tokunaga","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib10","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.molcel.2006.03.026","article-title":"Activation of IKK by TNFalpha requires site-specific ubiquitination of RIP1 and polyubiquitin binding by NEMO","volume":"22","author":"Ea","year":"2006","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib11","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/ncb1384","article-title":"Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]","volume":"8","author":"Wu","year":"2006","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib12","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/nature08247","article-title":"Direct activation of protein kinases by unanchored polyubiquitin chains","volume":"461","author":"Xia","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.10.018_bib13","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.molcel.2009.01.012","article-title":"Structural basis for recognition of diubiquitins by NEMO","volume":"33","author":"Lo","year":"2009","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib14","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/j.cell.2009.03.007","article-title":"Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation","volume":"136","author":"Rahighi","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib15","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1073\/pnas.0706552105","article-title":"Signal processing by its coil zipper domain activates IKK gamma","volume":"105","author":"Bloor","year":"2008","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.10.018_bib16","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0014-5793(03)00041-3","article-title":"Structure\u2013function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1","volume":"536","author":"Heyninck","year":"2003","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jmb.2009.10.018_bib17","doi-asserted-by":"crossref","first-page":"6131","DOI":"10.1038\/onc.2008.208","article-title":"ABINs inhibit EGF receptor-mediated NF-kappaB activation and growth of EGF receptor-overexpressing tumour cells","volume":"27","author":"Huang","year":"2008","journal-title":"Oncogene"},{"key":"10.1016\/j.jmb.2009.10.018_bib18","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1016\/j.cub.2007.07.041","article-title":"Optineurin negatively regulates TNFalpha-induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP","volume":"17","author":"Zhu","year":"2007","journal-title":"Curr. Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib19","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/humu.20739","article-title":"Alterations of the IKBKG locus and diseases: an update and a report of 13 novel mutations","volume":"29","author":"Fusco","year":"2008","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.jmb.2009.10.018_bib20","doi-asserted-by":"crossref","first-page":"6334","DOI":"10.1074\/jbc.M510118200","article-title":"A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor-mediated NF-kappa B activation","volume":"281","author":"Vinolo","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.018_bib21","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/j.str.2008.08.010","article-title":"Chaperone-assisted crystallography with DARPins","volume":"16","author":"Sennhauser","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2009.10.018_bib22","doi-asserted-by":"crossref","first-page":"27861","DOI":"10.1074\/jbc.M314278200","article-title":"The trimerization domain of NEMO is composed of the interacting C-terminal CC2 and LZ coiled-coil subdomains","volume":"279","author":"Agou","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.018_bib23","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1110\/ps.072924907","article-title":"Inhibition of NF-kappaB activation with designed ankyrin-repeat proteins targeting the ubiquitin-binding\/oligomerization domain of NEMO","volume":"16","author":"Wyler","year":"2007","journal-title":"Protein Sci."},{"key":"10.1016\/j.jmb.2009.10.018_bib24","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/prot.10286","article-title":"Structure validation by Calpha geometry: phi,psi and Cbeta deviation","volume":"50","author":"Lovell","year":"2003","journal-title":"Proteins"},{"key":"10.1016\/j.jmb.2009.10.018_bib25","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.jsb.2008.01.016","article-title":"Fifty years of coiled-coils and alpha-helical bundles: a close relationship between sequence and structure","volume":"163","author":"Parry","year":"2008","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib26","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1093\/hmg\/ddm237","article-title":"Identification of TRAF6-dependent NEMO polyubiquitination sites through analysis of a new NEMO mutation causing incontinentia pigmenti","volume":"16","author":"Sebban-Benin","year":"2007","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.jmb.2009.10.018_bib27","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.1038\/emboj.2009.241","article-title":"NEMO specifically recognizes K63-linked poly-ubiquitin chains through a new bipartite ubiquitin-binding domain","volume":"28","author":"Laplantine","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2009.10.018_bib28","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1074\/jbc.M806655200","article-title":"The zinc finger of NEMO is a functional ubiquitin-binding domain","volume":"284","author":"Cordier","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.018_bib29","doi-asserted-by":"crossref","first-page":"12482","DOI":"10.1021\/bi701137a","article-title":"Inhibitor kappaB kinase beta binding by inhibitor kappaB kinase gamma","volume":"46","author":"Drew","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.018_bib30","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1021\/bi702312c","article-title":"High-affinity interaction between IKKbeta and NEMO","volume":"47","author":"Lo","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.018_bib31","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1128\/MCB.23.6.2029-2041.2003","article-title":"Tetrameric oligomerization of IkappaB kinase gamma (IKKgamma) is obligatory for IKK complex activity and NF-kappaB activation","volume":"23","author":"Tegethoff","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib32","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1542\/peds.2004-1754","article-title":"NEMO mutations in 2 unrelated boys with severe infections and conical teeth","volume":"115","author":"Ku","year":"2005","journal-title":"Pediatrics"},{"key":"10.1016\/j.jmb.2009.10.018_bib33","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1084\/jem.20060085","article-title":"X-linked susceptibility to mycobacteria is caused by mutations in NEMO impairing CD40-dependent IL-12 production","volume":"203","author":"Filipe-Santos","year":"2006","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jmb.2009.10.018_bib34","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1016\/j.febslet.2009.09.028","article-title":"Crystal structure of the NEMO ubiquitin-binding domain in complex with Lys 63-linked di-ubiquitin","volume":"583","author":"Yoshikawa","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jmb.2009.10.018_bib35","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/embor.2009.55","article-title":"Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains","volume":"10","author":"Komander","year":"2009","journal-title":"EMBO Rep."},{"key":"10.1016\/j.jmb.2009.10.018_bib36","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/nsmb.1637","article-title":"Avid interactions underlie the Lys63-linked polyubiquitin binding specificities observed for UBA domains","volume":"16","author":"Sims","year":"2009","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib37","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1747-1028-2-11","article-title":"The ubiquitination code: a signalling problem","volume":"2","author":"Woelk","year":"2007","journal-title":"Cell Div."},{"key":"10.1016\/j.jmb.2009.10.018_bib38","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nsmb1064","article-title":"Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5","volume":"13","author":"Lee","year":"2006","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.10.018_bib39","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1016\/j.cell.2006.02.020","article-title":"Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin","volume":"124","author":"Penengo","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2009.10.018_bib40","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1038\/emboj.2009.160","article-title":"Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by tandem UIMs of RAP80","volume":"28","author":"Sato","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2009.10.018_bib41","doi-asserted-by":"crossref","first-page":"7055","DOI":"10.1074\/jbc.M309184200","article-title":"Solution conformation of Lys63-linked di-ubiquitin chain provides clues to functional diversity of polyubiquitin signaling","volume":"279","author":"Varadan","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.018_bib42","doi-asserted-by":"crossref","first-page":"2540","DOI":"10.1111\/j.1742-4658.2007.05788.x","article-title":"NEMO oligomerization in the dynamic assembly of the IkappaB kinase core complex","volume":"274","author":"Fontan","year":"2007","journal-title":"FEBS J."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609012583?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609012583?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,15]],"date-time":"2018-12-15T16:59:49Z","timestamp":1544893189000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283609012583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["S0022283609012583"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2009.10.018","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}