{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:13:18Z","timestamp":1791411198893,"version":"4.3.3"},"reference-count":45,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM81539"],"award-info":[{"award-number":["GM81539"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2010,1]]},"DOI":"10.1016\/j.jmb.2009.10.072","type":"journal-article","created":{"date-parts":[[2009,11,8]],"date-time":"2009-11-08T04:07:00Z","timestamp":1257653220000},"page":"430-443","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":84,"title":["Molecular Basis for the Recognition and Cleavages of IGF-II, TGF-\u03b1, and Amylin by Human Insulin-Degrading Enzyme"],"prefix":"10.1016","volume":"395","author":[{"given":"Qing","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marika","family":"Manolopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander B.","family":"Schilling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Jen","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2009.10.072_bib1","doi-asserted-by":"crossref","first-page":"2574","DOI":"10.1007\/s00018-008-8112-4","article-title":"Amyloid beta-degrading cryptidases: insulin degrading enzyme, presequence peptidase, and neprilysin","volume":"65","author":"Malito","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.jmb.2009.10.072_bib2","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S0083-6729(08)00622-5","article-title":"Structure, function, and regulation of insulin-degrading enzyme","volume":"80","author":"Hulse","year":"2009","journal-title":"Vitam. Horm."},{"key":"10.1016\/j.jmb.2009.10.072_bib3","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/nature05143","article-title":"Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism","volume":"443","author":"Shen","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.10.072_bib4","first-page":"608","article-title":"Insulin degradation: progress and potential","volume":"19","author":"Duckworth","year":"1998","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.jmb.2009.10.072_bib5","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1038\/sj.emboj.7601080","article-title":"The closed structure of presequence protease PreP forms a unique 10,000\u00a0\u00c53 chamber for proteolysis","volume":"25","author":"Johnson","year":"2006","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2009.10.072_bib6","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/S0969-2126(01)00621-9","article-title":"Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences","volume":"9","author":"Taylor","year":"2001","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2009.10.072_bib7","first-page":"190","article-title":"Pitrilysins\/inverzincins","volume":"vol. 3","author":"Maskos","year":"2004"},{"key":"10.1016\/j.jmb.2009.10.072_bib8","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.abb.2007.09.019","article-title":"Regulation of protein degradation by insulin-degrading enzyme: analysis by small interfering RNA-mediated gene silencing","volume":"468","author":"Fawcett","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.jmb.2009.10.072_bib9","first-page":"1","article-title":"The inactivation of insulin by tissue extracts; the distribution and properties of insulin inactivating extracts","volume":"20","author":"Mirsky","year":"1949","journal-title":"Arch. Biochem."},{"key":"10.1016\/j.jmb.2009.10.072_bib10","doi-asserted-by":"crossref","first-page":"4162","DOI":"10.1073\/pnas.0230450100","article-title":"Insulin-degrading enzyme regulates the levels of insulin, amyloid beta-protein, and the beta-amyloid precursor protein intracellular domain in vivo","volume":"100","author":"Farris","year":"2003","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.10.072_bib11","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1038\/nature05616","article-title":"A genome-wide association study identifies novel risk loci for type 2 diabetes","volume":"445","author":"Sladek","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.10.072_bib12","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/S0896-6273(03)00787-6","article-title":"Enhanced proteolysis of beta-amyloid in APP transgenic mice prevents plaque formation, secondary pathology, and premature death","volume":"40","author":"Leissring","year":"2003","journal-title":"Neuron"},{"key":"10.1016\/j.jmb.2009.10.072_bib13","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0968-0004(01)01876-X","article-title":"Insulin-degrading enzyme: embarking on amyloid destruction","volume":"26","author":"Kurochkin","year":"2001","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.jmb.2009.10.072_bib14","doi-asserted-by":"crossref","first-page":"7825","DOI":"10.1074\/jbc.M609168200","article-title":"Decreased catalytic activity of the insulin-degrading enzyme in chromosome 10-linked Alzheimer disease families","volume":"282","author":"Kim","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.072_bib15","first-page":"279","article-title":"Inhibition of insulin degradation by insulin-like growth factors I and II in human hepatoma (HepG2) cells","volume":"121","author":"Keller","year":"1989","journal-title":"Acta Endocrinol. (Copenh.)"},{"key":"10.1016\/j.jmb.2009.10.072_bib16","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1210\/endo-113-4-1525","article-title":"Inhibition of insulin degradation by insulin-like growth factors","volume":"113","author":"Misbin","year":"1983","journal-title":"Endocrinology"},{"key":"10.1016\/j.jmb.2009.10.072_bib17","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1210\/endo-128-3-1603","article-title":"Regulation of insulin, epidermal growth factor, and transforming growth factor-alpha levels by growth factor-degrading enzymes","volume":"128","author":"Gehm","year":"1991","journal-title":"Endocrinology"},{"key":"10.1016\/j.jmb.2009.10.072_bib18","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1432-1033.1991.tb16374.x","article-title":"Atrial natriuretic peptide (ANP) is a high-affinity substrate for rat insulin-degrading enzyme","volume":"202","author":"Muller","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jmb.2009.10.072_bib19","doi-asserted-by":"crossref","first-page":"25453","DOI":"10.1074\/jbc.M701590200","article-title":"Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE","volume":"282","author":"Im","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.072_bib20","doi-asserted-by":"crossref","first-page":"12822","DOI":"10.1021\/bi801192h","article-title":"Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme","volume":"47","author":"Malito","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.072_bib21","doi-asserted-by":"crossref","first-page":"14177","DOI":"10.1074\/jbc.M900068200","article-title":"Molecular basis of catalytic chamber-assisted unfolding and cleavage of human insulin by human insulin degrading enzyme","volume":"284","author":"Manolopoulou","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.072_bib22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3109\/07435808409035411","article-title":"Degradation of insulin-like growth factors I and II by a human insulin degrading enzyme","volume":"10","author":"Roth","year":"1984","journal-title":"Endocr. Res."},{"key":"10.1016\/j.jmb.2009.10.072_bib23","doi-asserted-by":"crossref","first-page":"5590","DOI":"10.1002\/j.1460-2075.1994.tb06896.x","article-title":"Solution structure of human insulin-like growth factor II; recognition sites for receptors and binding proteins","volume":"13","author":"Terasawa","year":"1994","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2009.10.072_bib24","doi-asserted-by":"crossref","first-page":"5484","DOI":"10.1021\/bi00236a022","article-title":"Solution structure of human insulin-like growth factor 1: a nuclear magnetic resonance and restrained molecular dynamics study","volume":"30","author":"Cooke","year":"1991","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.072_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/BJ20082102","article-title":"Insulin\/IGF-like signalling, the central nervous system and aging","volume":"418","author":"Broughton","year":"2009","journal-title":"Biochem. J."},{"key":"10.1016\/j.jmb.2009.10.072_bib26","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/nrc2536","article-title":"Insulin and insulin-like growth factor signalling in neoplasia","volume":"8","author":"Pollak","year":"2008","journal-title":"Nat. Rev., Cancer"},{"key":"10.1016\/j.jmb.2009.10.072_bib27","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1111\/j.1432-1033.1991.tb16050.x","article-title":"The solution structure of human transforming growth factor alpha","volume":"198","author":"Harvey","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jmb.2009.10.072_bib28","doi-asserted-by":"crossref","first-page":"11928","DOI":"10.1021\/bi00162a036","article-title":"Three-dimensional nuclear magnetic resonance structures of mouse epidermal growth factor in acidic and physiological pH solutions","volume":"31","author":"Kohda","year":"1992","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.072_bib29","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1002\/jcp.21635","article-title":"The epidermal growth factor receptor ligands at a glance","volume":"218","author":"Schneider","year":"2009","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.jmb.2009.10.072_bib30","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1210\/er.2007-0037","article-title":"Islet amyloid in type 2 diabetes, and the toxic oligomer hypothesis","volume":"29","author":"Haataja","year":"2008","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.jmb.2009.10.072_bib31","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1056\/NEJM200008103430607","article-title":"Islet amyloid and type 2 diabetes mellitus","volume":"343","author":"H\u00f6ppener","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jmb.2009.10.072_bib32","doi-asserted-by":"crossref","first-page":"10037","DOI":"10.1073\/pnas.181342398","article-title":"Electrostatics of nanosystems: application to microtubules and the ribosome","volume":"98","author":"Baker","year":"2001","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.10.072_bib33","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1038\/302500a0","article-title":"Transmission of conformational change in insulin","volume":"302","author":"Chothia","year":"1983","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.10.072_bib34","doi-asserted-by":"crossref","first-page":"7334","DOI":"10.1021\/bi00080a003","article-title":"Solution structure of human type-alpha transforming growth factor determined by heteronuclear NMR spectroscopy and refined by energy minimization with restraints","volume":"32","author":"Moy","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.10.072_bib35","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0022-2836(95)80058-1","article-title":"Solution structure of human insulin-like growth factor II. Relationship to receptor and binding protein interactions","volume":"248","author":"Torres","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.10.072_bib36","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0167-4838(96)00202-6","article-title":"Identification of the cleavage sites of transforming growth factor alpha by insulin-degrading enzymes","volume":"1338","author":"Hamel","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jmb.2009.10.072_bib37","doi-asserted-by":"crossref","first-page":"37314","DOI":"10.1074\/jbc.M305627200","article-title":"Kinetics of amyloid beta-protein degradation determined by novel fluorescence- and fluorescence polarization-based assays","volume":"278","author":"Leissring","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.10.072_bib38","doi-asserted-by":"crossref","first-page":"W512","DOI":"10.1093\/nar\/gkm307","article-title":"A server and database for dipole moments of proteins","volume":"35","author":"Felder","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jmb.2009.10.072_bib39","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1107\/S0907444996012255","article-title":"Refinement of macromolecular structures by the maximum-likelihood method","volume":"53","author":"Murshudov","year":"1997","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.10.072_bib40","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1107\/S0907444902015391","article-title":"The CCP4 molecular-graphics project","volume":"58","author":"Potterton","year":"2002","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.10.072_bib41","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.10.072_bib42","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: a new software suite for macromolecular structure determination","volume":"54","author":"Brunger","year":"1998","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.10.072_bib43","unstructured":"Delano, W. L. (2002). PyMOL. Pymol.sourceforge.net."},{"key":"10.1016\/j.jmb.2009.10.072_bib44","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1107\/S0907444993011333","article-title":"Detection, delineation, measurement and display of cavities in macromolecular structures","volume":"50","author":"Kleywegt","year":"1994","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.10.072_bib45","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1006\/jmbi.1993.1648","article-title":"Shape complementarity at protein\/protein interfaces","volume":"234","author":"Lawrence","year":"1993","journal-title":"J. Mol. Biol."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609013485?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609013485?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T06:10:09Z","timestamp":1762668609000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283609013485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["S0022283609013485"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2009.10.072","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2010,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular Basis for the Recognition and Cleavages of IGF-II, TGF-\u03b1, and Amylin by Human Insulin-Degrading Enzyme","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2009.10.072","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}