{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T18:05:43Z","timestamp":1770919543772,"version":"3.50.1"},"reference-count":60,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2010,2]]},"DOI":"10.1016\/j.jmb.2009.11.055","type":"journal-article","created":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T04:38:03Z","timestamp":1259815083000},"page":"634-645","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":26,"title":["Crystal Structures of Penicillin-Binding Proteins 4 and 5 from Haemophilus influenzae"],"prefix":"10.1016","volume":"396","author":[{"given":"Fumihiro","family":"Kawai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David I.","family":"Roper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gab-Jo","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang Yeon","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Unzai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Obayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam-Yong","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy R.H.","family":"Tame","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2009.11.055_bib1","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.mib.2003.10.002","article-title":"Role of penicillin-binding proteins in bacterial cell morphogenesis","volume":"6","author":"Popham","year":"2003","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib2","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/254516a0","article-title":"Penicillin-binding proteins and cell shape in E. coli","volume":"254","author":"Spratt","year":"1975","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.11.055_bib3","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1046\/j.1365-2958.2003.03607.x","article-title":"Bacterial shape","volume":"49","author":"Young","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0924-8579(96)00358-5","article-title":"Penicillin-binding proteins. Wall peptidoglycan assembly and resistance to penicillin: facts, doubts and hopes","volume":"8","author":"Ghuysen","year":"1997","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.jmb.2009.11.055_bib5","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1128\/jb.156.1.386-392.1983","article-title":"Evidence for multisite growth of Escherichia coli murein involving concomitant endopeptidase and transpeptidase activities","volume":"156","author":"Burman","year":"1983","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib6","doi-asserted-by":"crossref","first-page":"63","DOI":"10.2174\/1389201023378436","article-title":"Bifunctional penicillin-binding proteins: focus on the glycosyltransferase domain and its specific inhibitor moenomycin","volume":"3","author":"Di Guilmi","year":"2002","journal-title":"Curr. Pharm. Biotechnol."},{"key":"10.1016\/j.jmb.2009.11.055_bib7","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev.mi.45.100191.000345","article-title":"Serine beta-lactamases and penicillin-binding proteins","volume":"45","author":"Ghuysen","year":"1991","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib8","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1126\/science.1136611","article-title":"Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis","volume":"315","author":"Lovering","year":"2007","journal-title":"Science"},{"key":"10.1016\/j.jmb.2009.11.055_bib9","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.1128\/JB.188.7.2528-2532.2006","article-title":"Kinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus","volume":"188","author":"Terrak","year":"2006","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib10","doi-asserted-by":"crossref","first-page":"38096","DOI":"10.1074\/jbc.M508646200","article-title":"In vitro murein peptidoglycan synthesis by dimers of the bifunctional transglycosylase\u2013transpeptidase PBP1B from Escherichia coli","volume":"280","author":"Bertsche","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib11","doi-asserted-by":"crossref","first-page":"26985","DOI":"10.1074\/jbc.M604083200","article-title":"In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli","volume":"281","author":"Born","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib12","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/j.1574-6976.2008.00105.x","article-title":"The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis","volume":"32","author":"Sauvage","year":"2008","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jmb.2009.11.055_bib13","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1073\/pnas.54.4.1133","article-title":"Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-d-alanyl-d-alanine","volume":"54","author":"Tipper","year":"1965","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.11.055_bib14","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1111\/j.1365-2958.1991.tb00739.x","article-title":"Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition","volume":"5","author":"Korat","year":"1991","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib15","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.1021\/bi801993x","article-title":"Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4 (PBP4)","volume":"11","author":"Clarke","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib16","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1128\/JB.183.10.3055-3064.2001","article-title":"Contributions of PBP 5 and dd-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli","volume":"183","author":"Nelson","year":"2001","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib17","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1128\/JB.182.6.1714-1721.2000","article-title":"Penicillin binding protein 5 affects cell diameter, contour, and morphology of Escherichia coli","volume":"182","author":"Nelson","year":"2000","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib18","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1128\/JB.181.13.3981-3993.1999","article-title":"Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis","volume":"181","author":"Denome","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib19","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1086\/596317","article-title":"Penicillin-binding protein 7\/8 contributes to the survival of Acinetobacter baumannii in vitro and in vivo","volume":"199","author":"Russo","year":"2009","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jmb.2009.11.055_bib20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.tim.2008.04.006","article-title":"Physiological functions of d-alanine carboxypeptidases in Escherichia coli","volume":"16","author":"Ghosh","year":"2008","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib21","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jmb.2008.06.012","article-title":"Crystal structures of complexes of bacterial dd-peptidases with peptidoglycan-mimetic ligands: the substrate specificity puzzle","volume":"381","author":"Sauvage","year":"2008","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib22","doi-asserted-by":"crossref","first-page":"14614","DOI":"10.1021\/bi0350607","article-title":"Neisseria gonorrhoeae penicillin-binding protein 3 exhibits exceptionally high carboxypeptidase and beta-lactam binding activities","volume":"42","author":"Stefanova","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib23","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.jmb.2004.10.076","article-title":"Crystal structures of complexes between the R61 dd-peptidase and peptidoglycan-mimetic beta-lactams: a non-covalent complex with a \u201cperfect penicillin\u201d","volume":"345","author":"Silvaggi","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib24","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1021\/bi0268955","article-title":"The crystal structure of phosphonate-inhibited d-Ala-d-Ala peptidase reveals an analogue of a tetrahedral transition state","volume":"42","author":"Silvaggi","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib25","doi-asserted-by":"crossref","first-page":"7652","DOI":"10.1021\/ja036879a","article-title":"Mixed quantum mechanical\/molecular mechanical (QM\/MM) study of the deacylation reaction in a penicillin binding protein (PBP) versus in a class C beta-lactamase","volume":"126","author":"Gherman","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jmb.2009.11.055_bib26","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0167-4838(02)00311-4","article-title":"pH, inhibitor, and substrate specificity studies on Escherichia coli penicillin-binding protein 5","volume":"1597","author":"Stefanova","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jmb.2009.11.055_bib27","doi-asserted-by":"crossref","first-page":"8207","DOI":"10.1021\/bi0473004","article-title":"Crystal structure of Escherichia coli penicillin-binding protein 5 bound to a tripeptide boronic acid inhibitor: a role for Ser-110 in deacylation","volume":"44","author":"Nicola","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib28","doi-asserted-by":"crossref","first-page":"52826","DOI":"10.1074\/jbc.M310177200","article-title":"Crystal structure of wild-type penicillin-binding protein 5 from Escherichia coli: implications for deacylation of the acyl\u2013enzyme complex","volume":"278","author":"Nicholas","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib29","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1074\/jbc.M004471200","article-title":"Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-\u00c5 resolution","volume":"276","author":"Davies","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib30","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jmb.2008.12.001","article-title":"Structure of PBP-A from Thermosynechococcus elongatus, a penicillin-binding protein closely related to class A beta-lactamases","volume":"386","author":"Urbach","year":"2009","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib31","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/260451a0","article-title":"Fate of thiazolidine ring during fragmentation of penicillin by exocellular dd-carboxypeptidase\u2013transpeptidase of Streptomyces R61","volume":"260","author":"Frere","year":"1976","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2009.11.055_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.ppat.1000353","article-title":"Beta-lactam resistance response triggered by inactivation of a nonessential penicillin-binding protein","volume":"5","author":"Moya","year":"2009","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jmb.2009.11.055_bib33","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1574-6976.2007.00095.x","article-title":"Penicillin-binding proteins and beta-lactam resistance","volume":"32","author":"Zapun","year":"2008","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jmb.2009.11.055_bib34","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1021\/bi051533t","article-title":"Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics","volume":"45","author":"Kishida","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib35","doi-asserted-by":"crossref","first-page":"31249","DOI":"10.1074\/jbc.M503271200","article-title":"Crystal structure of the Actinomadura R39 dd-peptidase reveals new domains in penicillin-binding proteins","volume":"280","author":"Sauvage","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib36","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/protein\/8.2.127","article-title":"LIGPLOT: a program to generate schematic diagrams of protein\u2013ligand interactions","volume":"8","author":"Wallace","year":"1995","journal-title":"Protein Eng."},{"key":"10.1016\/j.jmb.2009.11.055_bib37","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.jmb.2005.10.030","article-title":"Crystal structure of penicillin-binding protein 1a (PBP1a) reveals a mutational hotspot implicated in beta-lactam resistance in Streptococcus pneumoniae","volume":"355","author":"Contreras-Martel","year":"2006","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib38","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1006\/jmbi.2000.3740","article-title":"The crystal structure of the penicillin-binding protein 2\u00d7 from Streptococcus pneumoniae and its acyl\u2013enzyme form: implication in drug resistance","volume":"299","author":"Gordon","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib39","doi-asserted-by":"crossref","first-page":"9532","DOI":"10.1021\/bi00029a030","article-title":"Binding of cephalothin and cefotaxime to d-ala-d-ala-peptidase reveals a functional basis of a natural mutation in a low-affinity penicillin-binding protein and in extended-spectrum beta-lactamases","volume":"34","author":"Kuzin","year":"1995","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2009.11.055_bib40","first-page":"870","article-title":"Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus","volume":"9","author":"Lim","year":"2002","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib41","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1073\/pnas.0407186102","article-title":"Active site restructuring regulates ligand recognition in class A penicillin-binding proteins","volume":"102","author":"Macheboeuf","year":"2005","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"10.1016\/j.jmb.2009.11.055_bib42","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1007\/s00018-002-8500-0","article-title":"The 2.4-\u00c5 crystal structure of the penicillin-resistant penicillin-binding protein PBP5fm from Enterococcus faecium in complex with benzylpenicillin","volume":"59","author":"Sauvage","year":"2002","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.jmb.2009.11.055_bib43","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/prot.340160406","article-title":"Crystal structure of Escherichia coli TEM1 beta-lactamase at 1.8\u00a0\u00c5 resolution","volume":"16","author":"Jelsch","year":"1993","journal-title":"Proteins"},{"key":"10.1016\/j.jmb.2009.11.055_bib44","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S0969-2126(01)00194-0","article-title":"Electrostatic analysis of TEM1 beta-lactamase: effect of substrate binding, steep potential gradients and consequences of site-directed mutations","volume":"3","author":"Swaren","year":"1995","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2009.11.055_bib45","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1007\/s00018-008-7591-7","article-title":"Substrate specificity of bacterial dd-peptidases (penicillin-binding proteins)","volume":"65","author":"Pratt","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.jmb.2009.11.055_bib46","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1128\/jb.174.10.3261-3269.1992","article-title":"Deletion of an additional domain located between SXXK and SXN active-site fingerprints in penicillin-binding protein 4 from Escherichia coli","volume":"174","author":"Mottl","year":"1992","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jmb.2009.11.055_bib47","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1042\/bj3030357","article-title":"Site-directed mutagenesis of proposed active-site residues of penicillin-binding protein 5 from Escherichia coli","volume":"303","author":"van der Linden","year":"1994","journal-title":"Biochem. J."},{"key":"10.1016\/j.jmb.2009.11.055_bib48","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1042\/BJ20050449","article-title":"A large displacement of the SXN motif of Cys115-modified penicillin-binding protein 5 from Escherichia coli","volume":"392","author":"Nicola","year":"2005","journal-title":"Biochem. J."},{"key":"10.1016\/j.jmb.2009.11.055_bib49","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.jmb.2009.02.024","article-title":"PBP active site flexibility as the key mechanism for beta-lactam resistance in pneumococci","volume":"387","author":"Contreras-Martel","year":"2009","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2009.11.055_bib50","doi-asserted-by":"crossref","first-page":"16463","DOI":"10.1074\/jbc.M313492200","article-title":"A PBP2x from a clinical isolate of Streptococcus pneumoniae exhibits an alternative mechanism for reduction of susceptibility to beta-lactam antibiotics","volume":"279","author":"Pernot","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib51","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s10156-008-0604-5","article-title":"Remarkably high prevalence of fts I gene mutations in Haemophilus influenzae isolates from upper respiratory tract infections in children of the Sapporo district, Japan","volume":"14","author":"Harimaya","year":"2008","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.jmb.2009.11.055_bib52","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.diagmicrobio.2008.12.015","article-title":"Canadian beta-lactamase-negative Haemophilus influenzae isolates showing decreased susceptibility toward ampicillin have significant penicillin binding protein 3 mutations","volume":"63","author":"Shuel","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jmb.2009.11.055_bib53","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.mib.2005.08.016","article-title":"Beta-lactam antibiotic resistance: a current structural perspective","volume":"8","author":"Wilke","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.jmb.2009.11.055_bib54","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1074\/jbc.M805761200","article-title":"Crystal structures of penicillin-binding protein 2 from penicillin-susceptible and-resistant strains of Neisseria gonorrhoeae reveal an unexpectedly subtle mechanism for antibiotic resistance","volume":"284","author":"Powell","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib55","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1074\/jbc.M706181200","article-title":"Common alterations in PBP1a from resistant Streptococcus pneumoniae decrease its reactivity toward beta-lactams: structural insights","volume":"283","author":"Job","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2009.11.055_bib56","doi-asserted-by":"crossref","first-page":"9293","DOI":"10.1021\/ja801727k","article-title":"Investigation of the mechanism of the cell wall dd-carboxypeptidase reaction of penicillin-binding protein 5 of Escherichia coli by quantum mechanics\/molecular mechanics calculations","volume":"130","author":"Shi","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jmb.2009.11.055_bib57","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jmb.2009.11.055_bib58","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1107\/S0907444905001617","article-title":"Likelihood-enhanced fast translation functions","volume":"61","author":"McCoy","year":"2005","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.11.055_bib59","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."},{"key":"10.1016\/j.jmb.2009.11.055_bib60","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1107\/S0907444996012255","article-title":"Refinement of macromolecular structures by the maximum-likelihood method","volume":"53","author":"Murshudov","year":"1997","journal-title":"Acta Crystallogr., Sect. D: Biol. Crystallogr."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609014491?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283609014491?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T06:10:09Z","timestamp":1762668609000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283609014491"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":60,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["S0022283609014491"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2009.11.055","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Crystal Structures of Penicillin-Binding Proteins 4 and 5 from Haemophilus influenzae","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2009.11.055","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}