{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:30:16Z","timestamp":1790206216924,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,2,2]],"date-time":"2019-02-02T00:00:00Z","timestamp":1549065600000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM101135"],"award-info":[{"award-number":["GM101135"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI102724"],"award-info":[{"award-number":["AI102724"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI057083"],"award-info":[{"award-number":["AI057083"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.jmb.2017.12.008","type":"journal-article","created":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T03:10:07Z","timestamp":1513393807000},"page":"310-321","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"title":["Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding"],"prefix":"10.1016","volume":"430","author":[{"given":"Jamie A.","family":"Moroco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Jeff","family":"Alvarado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan P.","family":"Staudt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Smithgall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Engen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmb.2017.12.008_bb0005","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1128\/JVI.72.1.121-132.1998","article-title":"Transgenic mice expressing human immunodeficiency virus type 1 in immune cells develop a severe AIDS-like disease","volume":"72","author":"Hanna","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0010","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12977-015-0187-z","article-title":"In vivo analysis of Nef's role in HIV-1 replication, systemic T cell activation and CD4(+) T cell loss","volume":"12","author":"Watkins","year":"2015","journal-title":"Retrovirology"},{"key":"10.1016\/j.jmb.2017.12.008_bb0015","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/1742-4690-9-44","article-title":"Nef functions in BLT mice to enhance HIV-1 replication and deplete CD4+CD8+ thymocytes","volume":"9","author":"Zou","year":"2012","journal-title":"Retrovirology"},{"key":"10.1016\/j.jmb.2017.12.008_bb0020","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/0092-8674(91)90097-I","article-title":"Importance of the nef gene for maintenance of high virus loads and for development of AIDS","volume":"65","author":"Kestler","year":"1991","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2017.12.008_bb0025","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1056\/NEJM199501263320405","article-title":"Brief report: absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection","volume":"332","author":"Kirchhoff","year":"1995","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jmb.2017.12.008_bb0030","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1126\/science.270.5238.988","article-title":"Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients","volume":"270","author":"Deacon","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.jmb.2017.12.008_bb0035","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1086\/430739","article-title":"Identification of attenuated variants of HIV-1 circulating recombinant form 01_AE that are associated with slow disease progression due to gross genetic alterations in the nef\/long terminal repeat sequences","volume":"192","author":"Kondo","year":"2005","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jmb.2017.12.008_bb0040","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.1128\/JVI.72.5.3646-3657.1998","article-title":"Grossly defective nef gene sequences in a human immunodeficiency virus type 1-seropositive long-term nonprogressor","volume":"72","author":"Salvi","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0045","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1742-4690-4-66","article-title":"Pathogenicity and immunogenicity of attenuated, nef-deleted HIV-1 strains in vivo","volume":"4","author":"Gorry","year":"2007","journal-title":"Retrovirology"},{"key":"10.1016\/j.jmb.2017.12.008_bb0050","doi-asserted-by":"crossref","first-page":"e523","DOI":"10.1016\/j.ddtec.2013.07.002","article-title":"Small molecule inhibitors of the HIV-1 virulence factor, Nef","volume":"10","author":"Smithgall","year":"2013","journal-title":"Drug Discov. Today Technol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0055","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/tra.12412","article-title":"HIV-1 Nef: taking control of protein trafficking","volume":"17","author":"Pereira","year":"2016","journal-title":"Traffic"},{"key":"10.1016\/j.jmb.2017.12.008_bb0060","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1084\/jem.177.6.1561","article-title":"Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection","volume":"177","author":"Benson","year":"1993","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jmb.2017.12.008_bb0065","doi-asserted-by":"crossref","first-page":"2633","DOI":"10.1128\/JVI.03230-13","article-title":"Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity","volume":"88","author":"Veillette","year":"2014","journal-title":"J. Virol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0070","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1742-4690-11-15","article-title":"HIV Nef and Vpu protect HIV-infected CD4+ T cells from antibody-mediated cell lysis through down-modulation of CD4 and BST2","volume":"11","author":"Pham","year":"2014","journal-title":"Retrovirology"},{"key":"10.1016\/j.jmb.2017.12.008_bb0075","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/34929","article-title":"HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes","volume":"391","author":"Collins","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2017.12.008_bb0080","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.bbagen.2015.01.003","article-title":"HIV-1 Nef: a master manipulator of the membrane trafficking machinery mediating immune evasion","volume":"1850","author":"Pawlak","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jmb.2017.12.008_bb0085","doi-asserted-by":"crossref","first-page":"11081","DOI":"10.1128\/JVI.74.23.11081-11087.2000","article-title":"Lentivirus Nef specifically activates Pak2","volume":"74","author":"Arora","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0090","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/S0960-9822(00)80086-X","article-title":"Identification of the Nef-associated kinase as p21-activated kinase 2","volume":"9","author":"Renkema","year":"1999","journal-title":"Curr. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0095","doi-asserted-by":"crossref","first-page":"17899","DOI":"10.1074\/jbc.272.29.17899","article-title":"SH3-mediated Hck tyrosine kinase activation and fibroblast transformation by the Nef protein of HIV-1","volume":"272","author":"Briggs","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2017.12.008_bb0100","doi-asserted-by":"crossref","first-page":"15718","DOI":"10.1074\/jbc.M114.572099","article-title":"The accessory factor Nef links HIV-1 to Tec\/Btk kinases in an SH3 domain-dependent manner","volume":"289","author":"Tarafdar","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2017.12.008_bb0105","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.chembiol.2012.11.005","article-title":"Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity","volume":"20","author":"Emert-Sedlak","year":"2013","journal-title":"Chem. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0110","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.chom.2007.03.004","article-title":"HIV-1 Nef assembles a Src family kinase-ZAP-70\/Syk-PI3K cascade to downregulate cell-surface MHC-I","volume":"1","author":"Hung","year":"2007","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.jmb.2017.12.008_bb0115","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/S0092-8674(00)81276-3","article-title":"Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain","volume":"85","author":"Lee","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.jmb.2017.12.008_bb0120","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0014-5793(01)02394-8","article-title":"Domain assembly, surface accessibility and sequence conservation in full length HIV-1 Nef","volume":"496","author":"Geyer","year":"2001","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jmb.2017.12.008_bb0125","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1099\/vir.0.81200-0","article-title":"Role of myristoylation and N-terminal basic residues in membrane association of the human immunodeficiency virus type 1 Nef protein","volume":"87","author":"Bentham","year":"2006","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0130","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nchembio.268","article-title":"HIV-1 Nef membrane association depends on charge, curvature, composition and sequence","volume":"6","author":"Gerlach","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0135","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/j.1460-2075.1995.tb07024.x","article-title":"Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4","volume":"14","author":"Saksela","year":"1995","journal-title":"EMBO J."},{"key":"10.1016\/j.jmb.2017.12.008_bb0140","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1038\/385650a0","article-title":"Activation of the Src-family tyrosine kinase Hck by SH3 domain displacement","volume":"385","author":"Moarefi","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.jmb.2017.12.008_bb0145","doi-asserted-by":"crossref","first-page":"28539","DOI":"10.1074\/jbc.M114.600031","article-title":"Interaction with the Src homology (SH3\u2013SH2) region of the Src-family kinase Hck structures the HIV-1 Nef dimer for kinase activation and effector recruitment","volume":"289","author":"Alvarado","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2017.12.008_bb0150","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.01754","article-title":"How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4","volume":"3","author":"Ren","year":"2014","journal-title":"eLife"},{"key":"10.1016\/j.jmb.2017.12.008_bb0155","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nsmb.2328","article-title":"Structural basis of evasion of cellular adaptive immunity by HIV-1 Nef","volume":"19","author":"Jia","year":"2012","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0160","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.jmb.2009.09.047","article-title":"HIV-1 Nef dimerization is required for Nef-mediated receptor downregulation and viral replication","volume":"394","author":"Poe","year":"2009","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0165","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1091\/mbc.e10-05-0470","article-title":"Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action","volume":"21","author":"Dikeakos","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.jmb.2017.12.008_bb0170","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1007\/s13361-016-1365-5","article-title":"Hydrogen exchange mass spectrometry of related proteins with divergent sequences: a comparative study of HIV-1 Nef allelic variants","volume":"27","author":"Wales","year":"2016","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.jmb.2017.12.008_bb0175","doi-asserted-by":"crossref","first-page":"7030","DOI":"10.1021\/acs.analchem.5b01725","article-title":"Membrane-associated conformation of HIV-1 Nef investigated with hydrogen exchange mass spectrometry at a Langmuir monolayer","volume":"87","author":"Pirrone","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jmb.2017.12.008_bb0180","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1021\/bi200197e","article-title":"Effects of HIV-1 Nef on human N-myristoyltransferase 1","volume":"50","author":"Morgan","year":"2011","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2017.12.008_bb0185","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jmb.2007.09.009","article-title":"Allosteric loss-of-function mutations in HIV-1 Nef from a long-term non-progressor","volume":"374","author":"Trible","year":"2007","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0190","doi-asserted-by":"crossref","first-page":"7733","DOI":"10.1021\/bi060438x","article-title":"Conformational features of the full-length HIV and SIV Nef proteins determined by mass spectrometry","volume":"45","author":"Hochrein","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2017.12.008_bb0195","doi-asserted-by":"crossref","first-page":"6382","DOI":"10.1021\/acs.biochem.5b00875","article-title":"Subtle dynamic changes accompany Hck activation by HIV-1 Nef and are reversed by an antiretroviral kinase inhibitor","volume":"54","author":"Wales","year":"2015","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2017.12.008_bb0200","doi-asserted-by":"crossref","first-page":"2670","DOI":"10.1074\/jbc.M116.770016","article-title":"Cell-based fluorescence complementation reveals a role for HIV-1 Nef protein dimerization in AP-2 adaptor recruitment and CD4 co-receptor down-regulation","volume":"292","author":"Shu","year":"2017","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmb.2017.12.008_bb0205","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1016\/j.str.2013.08.008","article-title":"Conformational transition of membrane-associated terminally acylated HIV-1 Nef","volume":"21","author":"Akgun","year":"2013","journal-title":"Structure"},{"key":"10.1016\/j.jmb.2017.12.008_bb0210","doi-asserted-by":"crossref","first-page":"15775","DOI":"10.1021\/bi048712f","article-title":"Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck","volume":"43","author":"Ye","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.jmb.2017.12.008_bb0215","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1021\/cb900195c","article-title":"Chemical library screens targeting an HIV-1 accessory factor\/host cell kinase complex identify novel antiretroviral compounds","volume":"4","author":"Emert-Sedlak","year":"2009","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.jmb.2017.12.008_bb0220","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/1742-4690-10-135","article-title":"Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen","volume":"10","author":"Trible","year":"2013","journal-title":"Retrovirology"},{"key":"10.1016\/j.jmb.2017.12.008_bb0225","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.93684","article-title":"Pharmacologic HIV-1 Nef blockade promotes CD8 T cell-mediated elimination of latently HIV-1-infected cells in vitro","volume":"2","author":"Mujib","year":"2017","journal-title":"JCI Insight"}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283617305880?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283617305880?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T06:14:44Z","timestamp":1762668884000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283617305880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["S0022283617305880"],"URL":"https:\/\/doi.org\/10.1016\/j.jmb.2017.12.008","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding","name":"articletitle","label":"Article Title"},{"value":"Journal of Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmb.2017.12.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}